JOURNAL OF EXPERIMENTAL BOTANY
·
2021
·Vol. 72
·期 12
·4577-4589
·
IF 6.992
Li Na,Bo Cunpei,Zhang Yuanyuan,Wang Lei
Leaf senescence can be triggered by multiple abiotic stresses including darkness, nutrient limitation, salinity, and drought. Recently, heatwaves have been occurring more frequently, and they dramatically affect plant growth and development. However, the underlying molecular networks of heat stress-induced leaf senescence remain largely uncharacterized. Here we showed that PHYTOCHROME INTERACTING FACTOR 4 (PIF4) and PIF5 proteins could efficiently promote heat stress-induced leaf senescence in Arabidopsis. Transcriptomic profiling analysis revealed that PIF4 and PIF5 are likely to function through multiple biological processes including hormone signaling pathways. Further, we characterized NAC019, SAG113, and IAA29 as direct transcriptional targets of PIF4 and PIF5. The transcription of NAC019, SAG113, and IAA29 changes significantly in daytime after heat treatment. In addition, we demonstrated that PIF4 and PIF5 proteins were accumulated during the recovery after heat treatment. Moreover, we showed that heat stress-induced leaf senescence is gated by the circadian clock, and plants might be more actively responsive to heat stress-induced senescence during the day. Taken together, our findings proposed important roles for PIF4 and PIF5 in mediating heat stress-induced leaf senescence, which may help to fully illustrate the molecular network of heat stress-induced leaf senescence in higher plants and facilitate the generation of heat stress-tolerant crops.
研究领域:
植物学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Lvyun Zhuang,Xiaopeng Wu,Daizhu Lyu,Mingyue Wang,Ruohao Zhou,Jia Song,Yu Rong
An investigation of the metabolism and transfer of imidacloprid (IMI) in banana plants and soil was performed using high-resolution mass spectrometry. Results indicated the presence of eight IMI metabolites in soil and leaves that resulted from hydroxylation of the imidazolidine ring, the reduction and loss of nitro groups, and oxidative cleavage of methylene bridges. Six metabolites, including 4/5-hydroxy IMI (4/5-hydroxy), IMI olefin (olefin), and 6-chloronicotinic acid (6-CNA), were detected in the fruits following leaf treatment, while only three were detected after soil treatment. Quantitative analysis showed that the total amount of imidacloprid and its metabolites transferred from leaves to fruits was higher than that transferred from soil to fruits. Therefore, leaf transfer was considered the main means by which IMI and its metabolites transferred to banana fruits. We found that adjuvants tank-mixed with IMI could reduce the total concentration of pesticide transfer from leaves to fruits, especially reducing the amount of metabolites transformed from the reduction and loss of nitro groups and oxidative cleavage of methylene bridges, thus reducing the pesticide residue in fruits and achieving the purpose of reducing the safety risk.
研究领域:
植物学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 24
·8881
·
IF 4.927
Qiang Zhang,Lianxiang Lu,Yanfei Zheng,Chengrong Qin,Yuexin Chen,Zhongjie Zhou
The extraction, characterization and antioxidant activity of polysaccharides fromChoerospondias axillarisleaves were investigated in the present study. Two purified polysaccharide fractions, CALP-1 and CALP-2, were isolated from crudeChoerospondias axillarisleaf polysaccharides (CALP) by DEAE-52 cellulose chromatography and Sephadex G-100 column chromatography. The characteristics of CAL-1 and CALP-2 were determined by using High-performance Gel Permeation Chromatography (HPGPC), High-Performance Anion-Exchange Chromatography, HPAEC (HPAEC-PAD) and Fourier transform infrared spectroscopy (FTIR). CALP-1 with molecular weight of 11.20 KDa was comprised of Rhamnose, Arabinose, Galactose, Glucose, Xylose, Mannose and galacturonic acid in a molar ratio of 5.16:2.31:5.50:27.18:1.00:0.76:1.07. CAL-2 with molecular weight of 8.03 KDa consisted of Rhamnose, Arabinose, Galactose, Glucose, and galacturonic acid at a ratio of 1.38:3.63:18.84:8.28:1.45. FTIR revealed that CALP-1 and CALP-2 were acidic polysaccharides. The antioxidant activity of crude CALP, CALP-1 and CALP-2 was evaluated in vitro. The fraction CALP-2 was demonstrated to be of polysaccharide nature containing a large percentage of Galactose but no Xylose and Mannose. The antioxidant activity assays showed that CALP-1 and CALP-2 exhibited antioxidant and scavenging activities on hydroxyl and DPPH radicals in vitro. Compared with pure polysaccharide, crude CALP exhibited stronger anti-oxidant activities. These results will provide a better understanding ofChoerospondiasaxillaris leaf polysaccharide and promote the potential applications ofChoerospondiasaxillaris leaf polysaccharide in the pharmacological field and as a natural antioxidant.Keywords:polysaccharide;Choerospondias axillarisleaves;purification;characterization;antioxidant
研究领域:
植物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2022
·
IF 3.388
Rui Shi,Bingjie Xiong,Shu He,Can Liu,Jiftah Ben-Asher,Abraham Rami Horowitz,Shu Wang,Xiahong He
Panax notoginseng is highly used in traditional Chinese medicine, and its root is valued for ginsenoside contents. However, evidence suggests that other plant parts such as fruit, leaves, and stem are also potential sources of bioactive compounds. Therefore, this study aimed at providing insight into the differential accumulation of metabolites in fruit, leaf, root, and stem tissues collected from P. notoginseng. A total of 808 metabolites from 11 major metabolite classes were identified. Furthermore, 32 Ginsenosides with six conserved Ginsenosides Rg1, Rf, St-3, R1, Ro, Rc, and five conserved notoginsenosides R1, K, M, E, Rb1, were identified with differential accumulation in fruit, leaf, stem, and root tissues. Several other metabolites known for their potential roles in human health were also identified as differentially accumulated in the tissues. Furthermore, the accumulation pattern of ginsenosides in different tissues is highly suggestive of utilizing fruit, leaf, and stem tissues along with roots for value addition of P. notoginseng.
研究领域:
植物学
·
药用植物研究
·
代谢组学
✓ 已验证
产品关联
DOI
高影响因子
Plant Signaling & Behavior
·
2022
·
IF 2.734
Rui Zhou,Menglong Fan,Mei Zhao,Xinqiang Jiang,Qinghua Liu
Leaves are the main vegetative organs of the aboveground part of plants and play an important role in plant morphogenesis. KNOTTED-LIKE HOMEOBOX (KNOX) plays a crucial role in regulating leaf cell fate and maintaining leaf development. In this study, we analyzed LtKNOX1 from Lilium tsingtauense and illustrated its function in transgenic plants. Tissue-specific expression analysis indicated that LtKNOX1 was highly expressed in stems, young flower buds, and shoot apical meristems (SAMs). Ectopic overexpression of LtKNOX1 in Nicotiana benthamiana suggested that transformants with mild phenotypes were characterized by foliar wrinkles and mildly curled leaves; transformants with intermediate phenotypes showed severely crimped blades and narrow leaf angles, and the most severe phenotypes lacked normal SAMs and leaves. Moreover, the expression levels of genes involved in the regulation of KNOX in transgenic plants were detected, including ASYMMETRIC LEAVES1, PIN-FORMED 1, GA20-oxidase, CUP-SHAPED COTYLEDON 2, CLAVATA 1 and WUSCHEL(WUS), and the expression of other genes were down-regulated except WUS. This study contributes to our understanding of the LtKNOX1 function.
研究领域:
植物学
·
遗传学
✓ 已验证
DOI
PubMed
高影响因子
CHEMISTRY & BIODIVERSITY
·
2024
·
IF 2.3
Zhengming Qian,Qi Huang,Mengqi Wu,Weiqi Yang,Zhixiong Wei,Xinjie Cheng,Deqiang Li
In the current study, a rapid online extraction combined with high-performance liquid chromatography (HPLC) antioxidant analysis approach was developed to explore the antioxidant ingredients in three different parts of Polygonum Chinense . A total of 22 chromatographic peaks were found, among which 8 components were found for the first time. And among these 22 peaks, 19 of them were demonstrated as antioxidants. Furthermore, all three parts from Polygonum Chinense exhibited antioxidant activity (13.19, 3.89, and 19.85 mg CE/g extract, for the leaf, stem, and flower, respectively) and the antioxidant activity values of the leaf and flower were more than 3 times that of the stem. The leaf, flower, and stem of Polygonum Chinense had 17, 13, and 15 antioxidant components, respectively. Among them, neochlorogenic acid (2) and quercetin-3-O-malonylglucoside (18) were considered as the main antioxidants in the leaf of Polygonum Chinense . In terms of flower, geraniin (10) and quercetin-3-O-malonylglucoside (18) were proved as the major antioxidants. This is the first report on the antioxidant components from different parts of Polygonum Chinense . It provides foundational scientific data for the continued exploration of chemical and pharmacological study of Polygonum Chinense , which is beneficial for the future product development and quality evaluation improvement of Polygonum Chinense . Graphical
研究领域:
植物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Chenyang Zhang,Xuewei Zhang,Xuerui Yang,Guangli Xiu,JM CHOVELON,Lei Zhou
The photochemical behaviors of fungicides under irradiation differ significantly between leaf surfaces and aqueous solutions. However, few studies have compared the photolysis of fungicides between these two environmental media. In this study, three typical fungicides—kresoxim-methyl (KM), pyraclostrobin (PAS), and cyprodinil (CRL)—were examined using carnauba wax to simulate leaf surfaces The photolysis kinetics, products, and pathways were systematically compared between aqueous solutions and wax films. On wax film, the photolysis rate constants of selected fungicides were observed to be significantly faster than in aqueous solution, with increases of 44, 5 and 36-fold for KM, PAS and CRL, respectively. The primary photolysis pathways on wax films involved photoisomerization and ether cleavage; while the hydroxylation process, commonly observed in aqueous systems, was absent. Toxicity assessments demonstrated that photolysis effectively reduced their ecotoxicities in both systems, with the photolysis on wax film documented as a more profound detoxification process. Moreover, wax film thickness and pesticide additives significantly influenced photodegradation kinetics of all the three fungicides. The present study highlighted the significant influence of environmental media on the photodegradation pathways of pesticides.
研究领域:
环境科学
·
农药化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2020
·Vol. 12
·期 33
·4130-4138
·
IF 2.596
Jingzhou Hou,Xianfeng Wang,Shiyu Lan,Chao Zhang,Changjun Hou,Qiang He,Danqun Huo
Carbon dots (CDs) having low cost and low toxicity and synthesized via a green route were applied to establish a fluorescent nanoprobe for the measurement of glyphosate. The synthesis was realized via a one-pot hydrothermal procedure using Sophora japonica leaves as the carbon source. It was found that electron transfer occurred between Fe3+ and the as-prepared CDs. Therefore, Fe3+ exhibited a specific dynamic-quenching toward CDs. However, the electron transfer process was inhibited by glyphosate. The fluorescence of the quenched CDs/Fe3+ system was recovered by the addition of glyphosate. It resulted from the strong complexation between Fe3+ and the functional groups (like –PO3H2 and –COOH) in the glyphosate molecule. These functional groups captured Fe3+ from the CD/Fe3+ system to reduce the electron transfer. With such a design, the rapid detection of glyphosate could be realized by this turn-on fluorescent sensor based on the CD/Fe3+ system. Under optimal conditions, the CD/Fe3+ system showed a concentration-dependent fluorescent response toward glyphosate in the linear range from 0.1 to 16 ppm. The limit of detection was calculated to be as low as 8.75 ppb (3σ/S). In addition, the successful detection of glyphosate in real samples with satisfactory recoveries exhibited a practical application of the CD/Fe3+ nanoprobe in food safety and environmental monitoring.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2017
·
IF 0.795
Xing-Li PEI,Yu-Yu HUANG,Can GONG,Xu XU
The ionic liquid butylamine 2,5-dihydroxybenzoate (DHB-BuN) was chosen as the matrix for improving reproducibility of oligosaccharides quantification, and imaging oligosaccharides in soybean and leaf by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS). In the experiment, ionic liquid matrix of methanol solution (20%, V/V ) was simply covered onto the sample slice, and MS imaging experiment was thus performed to analyze oligosaccharides and their distribution in soybean and soybean leaf in positive ion mode with laser power of 70%. As a result, the MALDI-MS analysis of three oligosaccharides including sucrose, raffinose and stachyose was performed using the ionic liquid matrix, and the reproducibility RSD was less than 3% for in-spot and less than 4% for spot-to-spot. The linear correlation coefficients ( R 2 ) of standard curves between MS peak intensity and oligosaccharides concentrations raging from 0.062 mg mL −1 to 1.00 mg mL −1 were all no less than 0.996, which showed a great potential in quantitative analysis of oligosaccharides by MALDI-MS. The matrix was then used for the imaging analysis of disaccharide, trisaccharide and tetrasaccharide on the surfaces of soybean chip and soybean leaf by MALDI-MS, and the mass spectrometric imaging of the three kinds of oligosaccharides was obtained with a spatial resolution of 150 μm. The result indicated that the distribution of three kinds of oligosaccharides in the soybean was roughly homogeneous, and mostly at its tip part in the leaf. The contents of the three oligosaccharides could be estimated by their MS peak intensity and above standard curve. This study suggested that the ionic liquid DHB-BuN matrix could be well applied in MALDI-MS imaging analysis of oligosaccharides in different samples.
研究领域:
分析化学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Plant Science
·
2018
·
IF 3.677
Guo Honggang,Sun Yucheng,Yan Hongyu,Li Chuanyou,Ge Feng
Elevated ozone (O3) can alter the phenotypes of host plants particularly in induction of leaf senescence, but few reports examine the involvement of phytohormone in O3-induced changes in host phenotypes that influence the foraging quality for insects. Here, we used an ethylene (ET) receptor mutant Nr and its wild-type to determine the function of the ET signaling pathway in O3-induced leaf senescence, and bottom-up effects on the performance of Bemisia tabaci in field open-top chambers (OTCs). Our results showed that elevated O3 reduced photosynthetic efficiency and chlorophyll content and induced leaf senescence of plant regardless of plant genotype. Leaf senescence in Nr plants was alleviated relative to wild-type under elevated O3. Further analyses of foliar quality showed that elevated O3 had little effect on phytohormone-mediated defenses, but significantly increased the concentration of amino acids in two plant genotypes. Furthermore, Nr plants had lower amino acid content relative to wild-type under elevated O3. These results provided an explanation of O3-induced increase in abundance of B. tabaci. We concluded that O3-induced senescence of plant was ET signal-dependent, and positive effects of O3-induced leaf senescence on the performance of B. tabaci largely resulted from changes of nutritional quality of host plants.
研究领域:
植物学
·
生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Runping Wang,Zaibo Yang,Wenzhi Lv,Zhijian Tan,Hongfu Zhang
The choline tryptophan ionic liquid (IL)-based aqueous biphasic system (ABS) was designed and applied to extract and separate flavonoids and iridoids from Eucommia ulmoides leaves. This ABS possessed a unique viscous layer enriching iridoids that can be directly removed at the interface between the IL-rich and salt-rich phases. The extraction yield of total flavonoids was optimized by RSM to obtain a predicted maximum of 2.91% at the IL concentration of 36.73%, liquid to solid ratio of 38, extraction temperature of 44 °C and extraction time of 105 min. The determined extraction yield of total flavonoids is 2.78% ± 0.12% under revised optimization conditions. The distribution coefficient K and extraction rate E of total flavonoids in the IL-rich phase reach the maximum of 129% and 98.53% at the K 3 PO 4 content of 26% in the ILABS, respectively. The HPLC analysis shows a high content of rutin in the flavonoids precipitate induced by water as an anti-solvent. Aucubin content reaches 15.19% in the intermediate viscous layer enriching iridoids. Hence, the designed process has the potential to be applied to the separation and enrichment of rutin and aucubin from other industrial crops.
研究领域:
植物化学
·
天然产物分离
✓ 已验证
DOI
高影响因子
Frontiers in Chemistry
·
2021
·
IF 5.221
Wu Panpan,Tang Xiaowen,Jian Rongchao,Li Jiahao,Lin Maoyu,Dai Huachao,Wang Kangpeng,Sheng Zhaojun,Chen Baizhong,Xu Xuetao,Li Chen,Lin Zhongze,Zhang Qingmin,Zheng Xi,Zhang Kun,Li Dongli,Hong Weiqian David
Two essential oils were isolated from discarded perfume lemon and leaves (Citrus limon (L.) Burm. F.) by hydro-distillation with good yield (0.044% for perfume lemon and 0.338% for leaves). Their biological activities were evaluated against five selected bacterial strains and Aedes albopictus (Ae. albopictus, Diptera: Culicidae). Chemical composition indicated that both essential oils were rich in essential phytochemicals including hydrocarbons, monoterpenes and sesquiterpene. These constituents revealed some variability among the oils displaying interesting chemotypes (R)-(+)-limonene (12.29–49.63%), citronellal (5.37–78.70%) and citronellol (2.98–7.18%). The biological assessments proved that the two essential oils had similar effect against bacterial (inhibition zones diameter ranging from 7.27 ± 0.06 to 10.37 ± 0.15 mm; MICs and MBCs ranging from 1.6 to 6.4 mg/mL); against Ae. albopictus larvae (LC50 ranging from 384.81 to 395.09 ppm) and adult mosquito (LD50 ranging from 133.059 to 218.962 μg/cm2); the activity of the two chemotypes ((R)-(+)-limonene and citronellal): larvae (LC50 ranging from 267.08 to 295.28 ppm), which were all presented in dose-dependent manners. Through this work, we have showcased that recycling and reusing of agriculture by-products, such as discarded perfume lemon and leaves can produce eco-friendly alternatives in bacterial disinfectants and mosquito control product.
研究领域:
植物化学
·
生物活性研究
·
农业废弃物利用
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）