CHEMICAL PHYSICS
·
2024
·
IF 2
Zhifeng Cai,Qi Liang,Yutong Wang,Xiao Wang,Lulu Jing,Yajie Cheng,Jinqi Liu,Zixuan Jia
Bright, blue fluorescent carbon quantum dots ( N -CDs) were carried out utilizing phenylenediamine and ascorbic acid as raw precursor through the hydrothermal method. The as-designed N -CDs suggested excitation-dependent characteristic changing from 340 nm to 380 nm. Moreover, Folic acid could availably quench the fluorescence intensity of the N -CDs. According to this experimental phenomenon, the sensitivity and selectivity of N -CDs for folic acid detection were developed. Under optimal experimental conditions, the as-prepared probe displayed excellent linearity with the folic acid concentration changing from 0.5 to 200 μM, and the limit of detection was 0.079 μM. Furthermore, the as-prepared probe indicated good sensitivity towards folic acid sensing in glucose and bovine serum samples. The recoveries of 95.9–104.8 % and 98.6–106.9 % were obtained in glucose and bovine serum samples, respectively. The corresponding relative standard deviation was lower than 2.74 % and 2.91 %, respectively. With preeminent sensitivity and selectivity, this as-developed nanosensor afforded a prospective platform for measuring folic acid in real samples. Furthermore, this N -CDs could be applied for the temperature detection.
研究领域:
分析化学
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生物医学工程
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
Carbon Letters
·
2025
·
IF 5.8
Ding Lixin,Sun Na,Zuo He,Luan Fang,Hou Lin,Chen Zhiyou,Sun Xue,Zheng Yuting,Dai Zongyu,Lv Yuguang,Hou Xingyu
Waste utilization is not only a way to protect the environment and realize green chemistry, but also a means to create novel materials. In this study, based on waste grape seeds as the biowaste-derived carbon dots (G-BCDs), a straightforward one-pot green method was employed for the rapid detection of folic acid (FA). Owing to the internal filter effect and the static mixing quenching mechanism, the sensing principle of G-BCDs was effectively quenched by FA. The results showed fluorescence at an emission wavelength of 415 nm upon excitation at 330 nm with a quantum yield of 1.5%. Particularly, the FA sensing assay obtained a broad linear range of 2–220 μM and the limit of detection was 0.48 μM. In addition, the fluorescence probe was successfully utilized for detecting FA in tablets, blood, and urine samples, yielding desirable results, which indicated promising applications in the fields of biological and pharmaceutical analysis. Graphical abstract
研究领域:
分析化学
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环境科学
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生物医学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2022
·Vol. 69
·期 3
·486-496
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IF 1.753
Xiao-Ye Zhan,Bin Wang,Li-Ping Yu
Glycidol-modified polyethylenimine (PEI-OH)-capped carbon dots (PEI-OH-CDs) were prepared and applied for selectively and sensitively detecting folic acid in food samples. PEI-OH-CDs were firstly synthesized by a one-pot hydrothermal method using PEI-OH and citric acid as precursors, and the transmission electron microscopy characterization reveals that the PEI-OH-CDs were of spherical shape with diameters of 2–3 nm and well dispersed in solution. The PEI-OH-CDs have high fluorescence and exhibit ultrastrong stability against pH, ion strength, and UV light irradiation. In a wide pH range higher than pH 6, the fluorescence of PEI-OH-CDs can be effectively quenched by folic acid through static quenching process and inner filter effect but is not affected by many typical chemicals including cations, anions, amino acids, and vitamins. A good linear relationship was found between the F 0 /F and the concentration of folic acid in the range from 0.1 to 100 μM, and the detection limit of folic acid is 0.07 μM. The PEI-OH-CDs were successfully applied for sensitive determination of folic acid in orange juice, fresh pear, grape spinach, and celery samples at pH 7.2, which is helpful to the stability of folic acid and provides a new strategy for accuracy detection of folic acid in food samples.
研究领域:
分析化学
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食品科学
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生物医学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Zhao,Jianhao,Yaling
The application of fluorescent carbon dots in bio-imaging has huge positive significance in the field of biomedicine. By taking this advantage, herein we prepared nitrogen, sulfur and iodine doped carbon dots (N,S,I-CDs) by a facile hydrothermal reaction using C 3 N 3 S 3 , potassium iodate (KIO 3 ) and ethylenediamine (EDA), and the obtained N,S,I-CDs show bright blue fluorescence with a high fluorescence quantum yield of about 32.4%. The prepared N, S, I-CDs could interact with the folic acid (FA) with high selectivity, lead to development of a high sensitive method for the FA detection from 0.1 to 175 μM wide linear range with a detection limit of 84 nM (S/N = 3) and also applied them in U-2 OS cells imaging. Moreover, this sensor possessed a good sensitivity, linearity and reversibility in the temperature range of 10–80 °C, and successfully applied for the temperature sensing in cell HT-29 samples. This investigation illustrates that as-prepared N, S, I-CDs probe may have great potential as a high-performance platform for the accurate recognition of temperature in cells and could provide a new tool for the detection of FA in cells.
研究领域:
生物医学
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分析化学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Jiali Qian,Feifei Quan,Fengjiao Zhao,Chengxin Wu,Zhaoyan Wang,Lei Zhou
Herein, we utilized aconitic acid (AA) as the precursor for preparing carbon dots (AA-CDs) by a facile hydrothermal reaction , and the resulting AA-CDs exhibited bright blue fluorescence with an absolute quantum yield of 56.5% in aqueous solution. Preliminary experiments showed that the prepared AA-CDs without further surface passivation or modification could selectively interact with folic acid (FA), resulting in significant fluorescence quenching. Based on this, a sensitive method for FA analysis was developed with a detection limit of 40 nM (S/N = 3), which was then successfully applied for the detection of FA in food and pharmaceutical samples with the average recoveries of 95.0–105.3%. Furthermore, a weak fluorescent nanoprobe (FA-AA-CDs) was fabricated based on the conjugated interaction of FA and AA-CDs, and its feasibility was evaluated as a fluorescence turn-on nanoprobe for targeted imaging of cancer cells using Hela, SMMC-7721, and A549 cells as models that expressed different levels of folate receptors (FRs) on the cell surface. The results exhibited that the fluorescence intensity of these cells was in accordance with their FRs expression levels, testifying its potential of FA-AA-CDs for targeted imaging of cancer cells. Subsequently, the internalization mechanism of FA-AA-CDs into cells was elucidated via receptor-mediated endocytosis using control experiments.
研究领域:
分析化学
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纳米医学
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肿瘤学
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分子影像
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药物递送
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生物传感
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细胞生物学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2016
·Vol. 6
·期 83
·79805-79810
·
IF 3.289
Zheng Juan Sun,Zhong Wei Jiang,Yuan Fang Li
In this contribution, a new in situ strategy for the preparation of AgNPs/MIL-101(Cr) hybrids as SERS substrates for folic acid detection was reported. In Tris–HCl buffer solution (pH = 8.5), poly(dopamine) (PDA) could be formed by self-polymerization of dopamine (DA), and was then effectively attached onto the surface of MIL-101(Cr) for further reduction of Ag+. By this strategy, AgNPs could be efficiently anchored on MIL-101(Cr) to form AgNPs/MIL-101(Cr) hybrids, which exhibited strong enrichment of folic acid (FA) due to electrostatic interaction, and the anchored AgNPs greatly enhanced the Raman signals of FA. Thus, the as-prepared hybrids have been used as excellent surface enhanced Raman scattering (SERS) substrates for the sensitive and selective detection of FA. Under the optimal experimental conditions, the SERS method shows good linearity in the range from 0.5 μM to 25 μM for FA with a correlation coefficient of 0.990, the detection limit was calculated to be 0.3 μM ± 0.02 μM. Moreover, it was successfully applied to detect FA in real samples with satisfactory results. These results demonstrated that the proposed SERS system has great practicability for chemical and biological assay applications, and the AgNPs/MIL-101(Cr) hybrids also offers potential for targeting cancer cells.
研究领域:
分析化学
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材料科学
·
生物医学
✓ 已验证
产品关联
DOI
高影响因子
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