TALANTA
·
2018
·
IF 4.244
Junli Zhang,Xuewei Zhao,Ming Xian,Chuan Dong,Shaomin Shuang
Early diagnosis is pivotal in subsequent prognosis and treatment of cancer. Herein, folic acid-conjugated carbon dots (FA-CDs) as a fluorescent nanoprobe were fabricated for identifying cancer cells visually. Green luminescent carbon dots (CDs) from active dry yeast (ADY) were readily prepared in scale-up to reach macroscopic production with a high yield of ~50% via a facile and rapid microwave approach. The as-prepared CDs were further combined with folic acid (FA) by covalent bonding to fabricate the FA-CDs for identification of cancer cells over-expressing folate receptor (FR). Experimental outcomes demonstrated that the resultant FA-CDs noninvasively entered into cancer cells via receptor-mediated endocytosis and could differentiate FR-positive HepG2 cells from a cell mixture by fluorescence imaging, which suggests a promising prospect of the FA-CDs as an efficient probe for cancer diagnosis and succeeding personalized therapy.
研究领域:
生物医学
·
材料科学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Chengxin Luan,Huan Wang,Qi Han,Xiaoyan Ma,Dagan Zhang,Yueshuang Xu,Baoan Chen,Minli Li,Yuanjin Zhao
Recovery of circulating tumor cells (CTCs) from cancer patients by an efficient CTCs capture and release method can greatly increase their application in diagnostics and treatment of cancers. In this paper, we presented a folic acid (FA)-functionalized hybrid photonic barcode for capture and release of CTCs. The hybrid photonic barcodes were formed by two nano-ordered parts, poly(ethylene glycol) diacrylate (PEGDA) inverse opal structure for sustaining integrity and methacrylated gelatin (GelMA) gel filler for conjugating FA molecules to mediate cell capture. The nano-ordered structures of the hybrid photonic barcodes not only increased contact area, but also decreased steric hindrance among FA molecules. Thus, the topographic interaction between the barcodes and CTCs was greatly enhanced. In addition, GelMA gel was soft and extracellular matrix (ECM) alike, which was beneficial to decrease impairment to CTCs during the CTCs-barcode interaction as well as preserving their viability. Demonstrated by four CTCs types, Hela (epithelial tissue, folate receptor positive, FR+), A02 (bone marrow, FR+), Raji (lymphoid tissue, FR+), and A549 (epithelial tissue, folate receptor negative, FR-), FR+ CTCs could be captured efficiently with reliability and specificity. The captured cells could be controllably released with high viability just by quick trypsinization. The whole processes were simple and efficient. These features indicated that the FA-functionalized hybrid photonic barcodes were promising for full recovery of CTCs from cancer patients, which was important for diagnosis and treatment of cancer.
研究领域:
生物医学工程
·
肿瘤学
·
材料科学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ORGANIC PROCESS RESEARCH & DEVELOPMENT
·
2024
·
IF 3.1
Yu Zhang,Minghui Xiao,Ranran Feng,Hong-Yu Zhang,Yuecheng Zhang,Jiquan Zhao
5-Methyltetrahydrofolate (5-MTHF) is an active form of folic acid (FA) and an essential micronutrient preventing anemia and numerous other adverse health conditions such as cardiovascular disease and cancer. Herein, a continuous flow sequence synthesis of 5-MTHF from FA was developed, involving three elementary steps: catalytic hydrogenation of FA to tetrahydrofolic acid (THFA), methylenation of THFA with formaldehyde, and hydrogenation of the methylenation intermediates to 5-MTHF. This process features an expeditious synthesis of 5-MTHF in an integrated flow platform with water as the sole solvent and without any intermediate purification. The overall yield of this three-step flow process was up to 53.54% in a total residence time of 71.4 min. The separated yield of 5-MTHF reached 49.3%.
研究领域:
生物化学
·
制药工程
·
营养学
✓ 已验证
产品关联
DOI
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2017
·
IF 3.151
Chaoyue Hao,Jianping Gao,Yongli Wu,Xiaoxue Wang,Ruiru Zhao,Shunkang Mei,Jiangbing Yang,Xiangang Zhai,Haixia Qiu
Folic acid (FA) supramolecular gel was prepared in a mixture solvent of N -methylpyrrolidone (NMP)/H 2 O at a 1/1 volume ratio by using FA as gelator with a concentration of 0.4% (w/v). In the mixture solvent, NMP was first of time used as a good solvent to tune the solvent properties in order to be able to induce formation of FA supramolecular gel. The folic acid-gelatin (FA-GA) hybrid gels were synthesized by adding different amount of gelatin (GA) into FA gelator. The gelation temperature (T Gel ) of the FA-GA hybrid gel is 30 °C which is much higher than that of FA gel (10 °C). The gel to sol transition temperature (T GS ) is about 58 °C, which demonstrates that the hybrid gel has good stability at room temperature . The FT-IR and UV–vis spectra of FA-GA hybrid gel indicate that GA involves in the self-assembly process of FA via hydrogen bonding and π-π interactions with FA molecules. Owing to the π-π interaction happened between FA and GA molecules the fluorescence intensity of FA-GA gel is four times as high as that of FA gel. SEM images shows that the hybrid gel has a helical-fiber network structure which would enhance the gel strength to a great extent. Good stability, large specific surface and porosity in three-dimensional network structure of the FA-GA hybrid gel make it a highly promising adsorbent for dye adsorption. The FA-GA hybrid gel is able to absorb 63.5% of Congo red (CR), 68% of methyl blue (MB) and 44% of methyl orange (MO) respectively from their solutions.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
Science of Advanced Materials
·
2021
·Vol. 13
·期 7
·1295-1303
·
IF 1.474
Liu Guangheng,Yang Xiangfeng,Niu Qiming,Sun Wenkui
ABSTRACT
A new type of difolate targeting nano-level ultrasound contrast agent ((folate molecule, FOL)2-TUAs) was prepared, so as to investigate its targeted binding effect with human breast cancer mammary carcinoma cells (MCF-7) in vitro. L-2-aminoadipic
acid (L-2-AD) as a branch unit was inserted at the hydroxyl end of distearoyl phosphatidylethanolamine (DISP)-PEG2000-COOH to construct a tree structure. At this time, the free hydroxyl group in the distearoyl phosphatidylethanolamine (DISP)-PEG2000-COOH structure modified the FOL with the
help of N-Hydroxysuccinimide/N,N'-dicyclohexylcarbodiimide (NHS/DCC). Each 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DISP-PEG2000) connected two FOLs to generate difolate targeted nanomaterials. Nano laser particle size (PS)
and Zeta potential analyzer (ZPA) were applied to analyze the physical characteristics of the material such as PS and dispersion, and the enhanced development effect in vitro was detected by the ultrasonic diagnostic instrument. Besides, the targeted binding ability of the contrast
agent based on this material to folate receptor (FR) overexpressing MCF-7 cells was analyzed by flow cytometry (FCM) and fluorescence microscope. In the experiment, hydrogen-1 nuclear magnetic resonance (1H NMR) demonstrated that (FOL)2-TUAs was successfully synthesized.
The surface of this material was round and uniformly distributed without aggregation. According to the relative number of FOL molecules, non-targeted nano-agent (U-TUA), monofolate targeted nano-agent (FOL-TUA), and difolate targeted nano-agent ((FOL)2-TUA) were obtained. The in
vitro imaging showed that different materials exhibited enhanced imaging effects in ultrasonic diagnostic equipment. FCM and fluorescence microscopy both indicated that the difolate TUA could achieve a good binding to MCF-7 cells. Most of the nano-agents were attached to the cell membrane,
surrounded by red fluorophore, namely increasing the FOL content of DISP-PEG2000 chain could enhance the targeted binding ability of tumor cells.
研究领域:
肿瘤学
·
医学影像学
·
药剂学
✓ 已验证
DOI
高影响因子
CHEMICAL PHYSICS
·
2024
·
IF 2
Zhifeng Cai,Qi Liang,Yutong Wang,Xiao Wang,Lulu Jing,Yajie Cheng,Jinqi Liu,Zixuan Jia
Bright, blue fluorescent carbon quantum dots ( N -CDs) were carried out utilizing phenylenediamine and ascorbic acid as raw precursor through the hydrothermal method. The as-designed N -CDs suggested excitation-dependent characteristic changing from 340 nm to 380 nm. Moreover, Folic acid could availably quench the fluorescence intensity of the N -CDs. According to this experimental phenomenon, the sensitivity and selectivity of N -CDs for folic acid detection were developed. Under optimal experimental conditions, the as-prepared probe displayed excellent linearity with the folic acid concentration changing from 0.5 to 200 μM, and the limit of detection was 0.079 μM. Furthermore, the as-prepared probe indicated good sensitivity towards folic acid sensing in glucose and bovine serum samples. The recoveries of 95.9–104.8 % and 98.6–106.9 % were obtained in glucose and bovine serum samples, respectively. The corresponding relative standard deviation was lower than 2.74 % and 2.91 %, respectively. With preeminent sensitivity and selectivity, this as-developed nanosensor afforded a prospective platform for measuring folic acid in real samples. Furthermore, this N -CDs could be applied for the temperature detection.
研究领域:
分析化学
·
生物医学工程
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Jiali Qian,Feifei Quan,Fengjiao Zhao,Chengxin Wu,Zhaoyan Wang,Lei Zhou
Herein, we utilized aconitic acid (AA) as the precursor for preparing carbon dots (AA-CDs) by a facile hydrothermal reaction , and the resulting AA-CDs exhibited bright blue fluorescence with an absolute quantum yield of 56.5% in aqueous solution. Preliminary experiments showed that the prepared AA-CDs without further surface passivation or modification could selectively interact with folic acid (FA), resulting in significant fluorescence quenching. Based on this, a sensitive method for FA analysis was developed with a detection limit of 40 nM (S/N = 3), which was then successfully applied for the detection of FA in food and pharmaceutical samples with the average recoveries of 95.0–105.3%. Furthermore, a weak fluorescent nanoprobe (FA-AA-CDs) was fabricated based on the conjugated interaction of FA and AA-CDs, and its feasibility was evaluated as a fluorescence turn-on nanoprobe for targeted imaging of cancer cells using Hela, SMMC-7721, and A549 cells as models that expressed different levels of folate receptors (FRs) on the cell surface. The results exhibited that the fluorescence intensity of these cells was in accordance with their FRs expression levels, testifying its potential of FA-AA-CDs for targeted imaging of cancer cells. Subsequently, the internalization mechanism of FA-AA-CDs into cells was elucidated via receptor-mediated endocytosis using control experiments.
研究领域:
分析化学
·
纳米医学
·
肿瘤学
·
分子影像
·
药物递送
·
生物传感
·
细胞生物学
✓ 已验证
DOI
高影响因子
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
·
2018
·
IF 2.297
Yunfei Wang,Jin Ren,Yonghua Liu,Rong Liu,Liangliang Wang,Qinglan Yuan,Jianming He,Yamin Nie,Jing Xu,Jingmou Yu
In this study, novel folic acid modified succinylated gelatin (FA-SG) micelles were prepared and used as drug carrier in folate-receptor (FR) targeted antitumor therapy. FA-SG copolymer was synthesized and characterized by 1 H NMR and fluorescence spectroscopy. Doxorubicin-loaded FA-SG (DOX-FA-SG) micelles were prepared by a dialysis method. The shape of DOX-FA-SG micelles was almost spherical by transmission electron microscopy analysis. The mean diameter of DOX-FA-SG micelles was 277 nm , and the DOX-loading content was 12.4%. In vitro drug release of DOX-loaded micelles showed sustained release behavior for 168 h. DOX-FA-SG demonstrated significantly greater cellular uptake and cytotoxicity against FR-positive MCF-7 cells than the non-targeted DOX-loaded micelles. Importantly, DOX-FA-SG micelles exhibited higher antitumor activity in MCF-7 tumor-bearing mice than doxorubicin hydrochloride (DOX⋅HCl) and DOX-loaded micelles without FA modification. Taken together, FA-SG micelles could be an effective carrier for targeted drug delivery .
研究领域:
药剂学
·
药物递送系统
·
癌症研究
✓ 已验证
产品关联
DOI
高影响因子
CHEMBIOCHEM
·
2015
·Vol. 16
·期 11
·1622-1628
·
IF 3.088
Rongqiang Liao,Shouhui Yi,Manshuo Liu,Wenling Jin,Bo Yang
Graphical Get smart: A smart targeting gene carrier for cancer-specific delivery has been successfully prepared by a “multilayer bricks-and-mortar” strategy. Carriers produced by two different self-assembly schemes were investigated for their ability to compact pDNA into nanoparticles. Cell viability studies show that the most suitable (Method A) material has lower cytotoxicity than 25 kDa bPEI and that transfection efficiency is maintained. A targeting gene carrier for cancer-specific delivery was successfully developed through a “multilayer bricks-mortar” strategy. The gene carrier was composed of adamantane-functionalized folic acid (FA-AD), an adamantane-functionalized poly(ethylene glycol) derivative (PEG-AD), and β-cyclodextrin-grafted low-molecular-weight branched polyethylenimine (PEI-CD). Carriers produced by two different self-assembly schemes, involving either precomplexation of the PEI-CD with the FA-AD and PEG-AD before pDNA condensation (Method A) or pDNA condensation with the PEI-CD prior to addition of the FA-AD and PEG-AD to engage host–guest complexation (Method B) were investigated for their ability to compact pDNA into nanoparticles. Cell viability studies show that the material produced by the Method A assembly scheme has lower cytotoxicity than branched PEI 25 kDa (PEI-25KD) and that the transfection efficiency is maintained. These findings suggest that the gene carrier, based on multivalent host–guest interactions, could be an effective, targeted, and low-toxicity carrier for delivering nucleic acid to target cells.
研究领域:
生物医学工程
·
分子生物学
·
药物递送系统
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEUROPHARMACOLOGY
·
2025
·
IF 4.6
Wen-Hui Ni,Ke Wang,Yun Wang,Jia Lu,Chun-Ting Lu,Wen Rong,Yi-Feng Gu,Wen-Juan Qian,Hai-Long Zhang
Chronic inflammatory pain is a top priority for arthritis patients seeking medical care. Despite the availability of NSAIDs and glucocorticoids, pain management becomes increasingly challenging due to central and peripheral sensitization. Previous studies have shown that Matrix metalloproteinase 2 (MMP2) promotes neuroinflammation by cleaving extracellular matrix proteins and activating pro-inflammatory cytokines. Folic acid acts as a promising candidate for the treatment of neuroinflammatory diseases due to its neuroprotective effects. However, the role of folic acid in inflammatory pain remains unclear. This study investigated the analgesic mechanisms of folic acid in inflammatory pain. Adult rats underwent inflammatory pain by injecting complete freund's adjuvant (CFA) into the right hindpaw. Behavioral tests were used to assess the paw withdrawal threshold (PWT) and paw withdrawal latency (PWL). The results demonstrated that CFA injection induced abnormal mechanical and thermal pain and increased MMP2 expression in L3-L5 DRG and SDH of CFA rats. MMP2 was mainly expressed in neurons rather than glial cells in L3-L5 DRG of CFA rats. We further discovered that MMP2 inhibitor auraptene or knockdown alleviated inflammatory pain in CFA rats. Interestingly, we observed that long-term folic acid treatment reversed MMP2 overexpression, resulting in sustained relief of chronic inflammatory pain. Consistently, long-term folic acid treatment also relieved pain-induced anxiety. These results indicated that folic acid had a protective role in chronic inflammatory pain and pain-induced anxiety by repressing MMP2 expression. Folic acid or auraptene might be promising therapeutic options for the treatment of chronic inflammatory pain.
研究领域:
药理学
·
神经科学
·
炎症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Shanting Lin,Xiao Hu,Shengjun Chen,Hui Huang,Xiaoshan Long,Zhenxing Li
The aims of this study were to investigate the effect of precise phosphorylation of Ser in GL-Hyp-GPSGEEGKR on its Fe 2+ chelating capacity, and the effects and mechanisms of folic acid coupling to GL-Hyp-GPS (PO4) GEEGKR on its resistance against degradation by gastrointestinal enzymes. The results demonstrated that GL-Hyp-GPS (PO4) GEEGKR exhibited significantly enhanced Fe 2+ chelating capability compared to GL-Hyp-GPSGEEGKR. This was attributed to the phosphorylation of Ser introducing more Fe 2+ chelating sites. Furthermore, the results of UV–Vis, FTIR, and 1 H NMR showed that folic acid has successfully coupled with GL-Hyp-GPS (PO4) GEEGKR to generate folic acid-GL-Hyp-GPS (PO4) GEEGKR. Folic acid-GL-Hyp-GPS (PO4) GEEGKR demonstrated superior resistance against degradation by gastrointestinal enzymes. This may be attributed to the coupling of folic acid altering the original zeta potential of the peptide and the steric hindrance effect of folic acid hindering the interaction between enzymes and peptide. These findings can provide valuable insights for the production of high Fe 2+ chelating peptides that resist gastrointestinal enzyme degradation.
研究领域:
生物化学
·
药学
·
营养科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2024
·Vol. 149
·期 24
·5863-5870
·
IF 3.6
Jie Huang,Ruicheng Xu,Qiaoting Yang,Kang Tao,Dan Shan
The purpose of the present study is to develop an easy and effective one-step pyrolytic method for synthesizing carbon dots (CDs) derived from folic acid (FA), denoted herein as FACDs. This method is green, inexpensive, and simplifies the synthesis of fluorescent carbon nanomaterials with enhanced and stable fluorescence. Moreover, the as-prepared FACDs are effective in the sensitive and selective detection of cysteine (Cys) by such a “turn-on” mechanism of fluorescence. FACDs have been able to realize a low detection limit of 1.7 × 10−7 M and a wide linear range from 5.0 × 10−7 to 8.0 × 10−5 M, successfully restoring quenched fluorescence in the presence of Cys. Obtained results confirm FACDs as a trustful high-performance sensor for various biological and environmental applications and pave the way for facile and efficient fluorescence-based detection platforms.
研究领域:
材料科学
·
分析化学
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）