JOURNAL OF ETHNOPHARMACOLOGY
·
2021
·
IF 4.36
Han Zhou,Tao Hou,Zhenhua Gao,Xiujie Guo,Chaoran Wang,Jixia Wang,Yanfang Liu,Xinmiao Liang
Ethnopharmacological relevance Dopamine receptors are long-standing primary targets in the treatment of mental diseases and there is growing evidence that suggests relationships between obesity and the dopamine system, especially dopamine D1 and D2 receptors. Leaves of Nelumbo nucifera Gaertn. (lotus leaves) have been medically used for helping long-term maintenance of weight loss. Whether and how components of lotus leaves function through the dopamine receptors remains unclear. Aim of the study This work aimed to discover dopamine receptor-active alkaloids isolated from the lotus leaves, to evaluate their potencies and to analyze their structure activity relationship . Materials and methods Dried lotus leaves were prepared and total extract was divided into alkaloids and flavones . Eight alkaloids were separated and characterized by a combination of high-performance liquid chromatography, quadrupole time-of-flight mass spectrometry and nuclear magnetic resonance, and assayed by a fluorometric imaging plate reader platform. Human embryonic kidney 239 cell lines expressing dopamine D1, D2 and serotonin 2A (5-HT2A) receptors, respectively, were cultured and used in the assay. Results Alkaloids in the lotus leaves were the bioactive phytochemicals and inhibited dopamine from accessing the D1 and D2 receptors. All eight compounds functioned as D1-receptor antagonists and except N-nornuciferine, seven alkaloids functioned as D2-receptor antagonists. (S)-coclaurine and (R)-coclaurine are optical isomers and antagonized both D1 and D2 with equivalent potencies, suggesting that the optical rotation of the methylene linker in the monobenzyl isoquinoline backbone did not influence their activity. Among the eight alkaloids, O-nornuciferine was the potent antagonist to both receptors (the lowest IC 50 values, D1: 2.09 ± 0.65 μM and D2: 1.14 ± 0.10 μM) while N-nornuciferine was found to be the least potent as it moderately antagonized D1 and was inactive on D2. O-nornuciferine was also a 5-HT2A antagonist (IC 50 ~20 μM) while N-nornuciferine had no activity. These hinted the importance of a methyl group attached to the nitrogen atom in the aporphine backbone. Armepavine showed a nearly 10-fold selectivity to D2. Conclusions In this work, eight alkaloids were isolated from the leaves of Nelumbo nucifera Gaertn. and assayed on the D1 and D2 receptors. They were D1/D2 antagonists with IC 50 values in the mid- to low-micromolar range and O-nornuciferine was the most potent alkaloid among the eight. This family of alkaloids was biochemically evaluated on the dopamine receptors by the same platform for the first time.
研究领域:
药理学
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植物化学
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神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
·
IF 5.279
Cong Qiu,Yang Liu,Yangbao Wu,Linguo Zhao,Jianjun Pei
Prenyl groups increase the lipophilicity of flavonoids, endowing them with a special activity, selectivity, and pharmacological properties by prenylation. Herein, a novel prenyltransferase (ShFPT) gene from Streptomyces sp. NT11 was expressed in Escherichia coli, and its biochemical characteristics were determined. ShFPT exhibited high selectivity to prenylate naringenin at C-6 to generate 6-prenylnaringenin. The optimal activity was observed at pH 6.0 and 55 °C. The Kcat and Km for naringenin were 0.0095 s–1 and 0.20 mM, respectively. Several promiscuous kinase and isopentenyl phosphate kinase genes were screened to develop the most efficient dimethylallyl diphosphate (DMAPP) synthesis pathway for 6-prenylnaringenin synthesis in E. coli. The 6-prenylnaringenin production was improved by changing the induction strategies and optimizing the bioconversion conditions. Finally, 6-prenylnaringenin production reached the highest yield of 69.9 mg/L with average productivity of 4.0 mg/L/h after 16 h incubation, which is the highest yield for any prenylated flavonoid reported to date in E. coli. Therefore, this study provides an efficient method for 6-prenylnaringenin production and reveals the DMAPP synthesis pathway.
研究领域:
生物化学
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微生物学
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药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2018
·
IF 4.244
Junli Zhang,Xuewei Zhao,Ming Xian,Chuan Dong,Shaomin Shuang
Early diagnosis is pivotal in subsequent prognosis and treatment of cancer. Herein, folic acid-conjugated carbon dots (FA-CDs) as a fluorescent nanoprobe were fabricated for identifying cancer cells visually. Green luminescent carbon dots (CDs) from active dry yeast (ADY) were readily prepared in scale-up to reach macroscopic production with a high yield of ~50% via a facile and rapid microwave approach. The as-prepared CDs were further combined with folic acid (FA) by covalent bonding to fabricate the FA-CDs for identification of cancer cells over-expressing folate receptor (FR). Experimental outcomes demonstrated that the resultant FA-CDs noninvasively entered into cancer cells via receptor-mediated endocytosis and could differentiate FR-positive HepG2 cells from a cell mixture by fluorescence imaging, which suggests a promising prospect of the FA-CDs as an efficient probe for cancer diagnosis and succeeding personalized therapy.
研究领域:
生物医学
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材料科学
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肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2023
·
IF 5.6
Zeng Jiajing,Yang Mengyu,Deng Jing,Zheng Dongyang,Lai Zhongxiong,Wang-Pruski Gefu,XuHan Xu,Guo Rongfang
XG Chinese kale (Brassica oleracea cv. ‘XiangGu’) is a variety of Chinese kale and has metamorphic leaves attached to the true leaves. Metamorphic leaves are secondary leaves emerging from the veins of true leaves. However, it remains unknown how the formation of metamorphic leaves is regulated and whether it differs from normal leaves. BoTCP25 is differentially expressed in different parts of XG leaves and respond to auxin signals. To clarify the function of BoTCP25 in XG Chinese kale leaves, we overexpressed BoTCP25 in XG and Arabidopsis, and interestingly, its overexpression caused Chinese kale leaves to curl and changed the location of metamorphic leaves, whereas heterologous expression of BoTCP25 in Arabidopsis did not show metamorphic leaves, but only an increase in leaf number and leaf area. Further analysis of the expression of related genes in Chinese kale and Arabidopsis overexpressing BoTCP25 revealed that BoTCP25 could directly bind the promoter of BoNGA3, a transcription factor related to leaf development, and induce a significant expression of BoNGA3 in transgenic Chinese kale plants, whereas this induction of NGA3 did not occur in transgenic Arabidopsis. This suggests that the regulation of Chinese kale metamorphic leaves by BoTCP25 is dependent on a regulatory pathway or elements specific to XG and that this regulatory element may be repressed or absent from Arabidopsis. In addition, the expression of miR319’s precursor, a negative regulator of BoTCP25, also differed in transgenic Chinese kale and Arabidopsis. miR319’s transcrips were significantly up-regulated in transgenic Chinese kale mature leaves, while in transgenic Arabidopsis, the expression of miR319 in mature leaves was kept low. In conclusion, the differential expression of BoNGA3 and miR319 in the two species may be related to the exertion of BoTCP25 function, thus partially contributing to the differences in leaf phenotypes between overexpressed BoTCP25 in Arabidopsis and Chinese kale.
研究领域:
植物生物学
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分子遗传学
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发育生物学
✓ 已验证
DOI
PubMed
高影响因子
Agronomy-Basel
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2022
·Vol. 13
·期 1
·121
·
IF 3.949
Xiaoyun Huang,Hongxing Chen,Hui Chen,Chengwu Fan,Yueying Tai,Xiaoran Chen,Wang Zhang,Tengbing He,Zhenran Gao
The spatiotemporal heterogeneity of rice canopy leaves at different leaf positions is very important for non-destructive monitoring under Cadmium (Cd) stress, and is one of the key problems that need to be solved urgently in modern agriculture. This study aims to explore the temporal and spatial heterogeneity of chlorophyll content and fluorescence in rice canopy leaves under different Cd stress concentrations. The responses of the relative chlorophyll content (SPAD) and Chlorophyll fluorescence (ChlF) parameters to Cd stress in the tillering, heading, and filling stages were analyzed through pot experiments with three Cd concentrations: 0.20 mg·kg−1(CK), 0.60 mg·kg−1(Cd1), and 1.60 mg·kg−1(Cd2). Results showed that the canopy leaf SPAD value increased with increasing Cd concentration. Compared with CK, the leaf SPAD value in the Cd1 and Cd2 treatments were 1.91–3.45% and 5.52–12.01% lower, respectively. The SPAD value of the lower leaves was higher in the tillering stage, while the SPAD value of the upper leaves was higher in the heading and filling stages. The non-photochemical quenching coefficient (NPQ) of the third leaf (D3), the initial fluorescence yield (Fo) of the second leaf (D2), and the maximum photochemical efficiency (Fv/Fm) of photosystem II (PSII) in the tillering stage; the NPQ, Fo, and Fv/Fm of the D3 in the heading stage; and the NPQ, Fo, Fv/Fm, and Fv/Fm of the D2 in the filling stage were more sensitive to Cd stress than the other leaves and ChlF parameters.Keywords:cadmium;rice;relative chlorophyll content;chlorophyll fluorescence;photosynthesis
研究领域:
植物生理学
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环境科学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2023
·Vol. 24
·期 5
·4640
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IF 5.6
Anqi Peng,Keke Yu,Shuwei Yu,Yingying Li,Hao Zuo,Ping Li,Juan Li,Jianan Huang,Zhonghua Liu,Jian Zhao
Tea plants have adapted to grow in tropical acidic soils containing high concentrations of aluminum (Al) and fluoride (F) (as Al/F hyperaccumulators) and use secret organic acids (OAs) to acidify the rhizosphere for acquiring phosphorous and element nutrients. The self-enhanced rhizosphere acidification under Al/F stress and acid rain also render tea plants prone to accumulate more heavy metals and F, which raises significant food safety and health concerns. However, the mechanism behind this is not fully understood. Here, we report that tea plants responded to Al and F stresses by synthesizing and secreting OAs and altering profiles of amino acids, catechins, and caffeine in their roots. These organic compounds could form tea-plant mechanisms to tolerate lower pH and higher Al and F concentrations. Furthermore, high concentrations of Al and F stresses negatively affected the accumulation of tea secondary metabolites in young leaves, and thereby tea nutrient value. The young leaves of tea seedlings under Al and F stresses also tended to increase Al and F accumulation in young leaves but lower essential tea secondary metabolites, which challenged tea quality and safety. Comparisons of transcriptome data combined with metabolite profiling revealed that the corresponding metabolic gene expression supported and explained the metabolism changes in tea roots and young leaves via stresses from high concentrations of Al and F. The study provides new insight into Al- and F-stressed tea plants with regard to responsive metabolism changes and tolerance strategy establishment in tea plants and the impacts of Al/F stresses on metabolite compositions in young leaves used for making teas, which could influence tea nutritional value and food safety.Keywords:aluminum and fluoride;organic acid;secondary metabolite;tea quality;tea safety
研究领域:
植物生理学
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代谢组学
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食品安全
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农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLANT CELL AND ENVIRONMENT
·
2021
·Vol. 44
·期 11
·3681-3699
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IF 7.228
Yushan Zhai,Quan Yuan,Shiyou Qiu,Saisai Li,Miaomiao Li,Hongying Zheng,Guanwei Wu,Yuwen Lu,Jiejun Peng,Shaofei Rao,Jianping Chen,Fei Yan
Chloroplasts play crucial roles in plant defence against viral infection. We now report that chloroplast NADH dehydrogenase-like (NDH) complex M subunit gene ( NdhM ) was first up-regulated and then down-regulated in turnip mosaic virus (TuMV)-infected N. benthamiana . NbNdhM -silenced plants were more susceptible to TuMV, whereas overexpression of NbNdhM inhibited TuMV accumulation. Overexpression of NbNdhM significantly induced the clustering of chloroplasts around the nuclei and disturbing this clustering facilitated TuMV infection, suggesting that the clustering mediated by NbNdhM is a defence against TuMV. It was then shown that NbNdhM interacted with TuMV VPg, and that the NdhMs of different plant species interacted with the proteins of different viruses, implying that NdhM may be a common target of viruses. In the presence of TuMV VPg, NbNdhM, which is normally localized in the nucleus, chloroplasts, cell periphery and chloroplast stromules, colocalized with VPg at the nucleus and nucleolus, with significantly increased nuclear accumulation, while NbNdhM-mediated chloroplast clustering was significantly impaired. This study therefore indicates that NbNdhM has a defensive role in TuMV infection probably by inducing the perinuclear clustering of chloroplasts, and that the localization of NbNdhM is altered by its interaction with TuMV VPg in a way that promotes virus infection.
研究领域:
植物病毒学
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分子植物病理学
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叶绿体生物学
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植物免疫学
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分子病毒学
✓ 已验证
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yingjuan Chen,Yuxin Han,Shiqiang He,Qinghua Cheng,Huarong Tong
Albino tea cultivars with distinct leaf colors have rarely been used to explore the metabolic mechanism of leaf color formation. Carotenoids and chlorophyll a re primary pigments affecting leaf color with differential metabolic mechanisms in albino tea resources. TEM analysis showed that in albino stage, much less chloroplasts with abnormal structure were observed in white and yellow tea leaves, which corresponded to the significant reduction of total chlorophyll, Chl a and Chl b, and increase of Chl a/b, resulting in tea leaves to lose their green color. Compared to green tea ‘Fudingdabaicha’ (FD), three albino tea cultivars had higher number of carotenoids, but much lower contents. For albino mutants, significant lower α-Carotene, α-cryptoxanthin and lutein involved in lutein biosynthesis and remarkable higher zeaxanthin ( P < 0.05) were responsible for the leaf yellowing of ‘Huangjinya’ (HJY), while zeaxanthin degradation and accumulation of antheraxanthin, violaxanthin and neoxanthin contribute to the white appearance of ‘Jibai’ (JB).
研究领域:
植物生理学
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代谢组学
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茶叶科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Insects
·
2022
·Vol. 13
·期 11
·1056
·
IF 3.139
Nanxia Fu,Fida Hussain Magsi,Yingjie Zhao,Xiaoming Cai,Zhaoqun Li,Lei Bian,Chunli Xiu,Zongmao Chen,Zongxiu Luo
Simple SummaryThe tea tortrixArchips strojnyis a major pest of spring tea in China. Using sex pheromone-based mass trapping or mating disruption techniques to control this pest can minimize excessive pesticide usage, thereby maintaining pesticide residues within the safety standard for spring tea products. However, the sex pheromone components of this species remain elusive. In this study, we identified these components: laboratory assays showed that two bioactive compounds in female sex pheromone gland extracts, namely, (Z)-11-tetradecenyl acetate (Z11-14:Ac) and (Z)-11-tetradecenyl alcohol (Z11-14:OH), could elicit male moth antennal responses. A field trapping assay demonstrated that Z11-14:Ac was the attractant in the sex pheromone, while Z11-14:OH seemed to be an antagonist. Overall, our results indicated that lures baited with 1 mg of Z11-14:Ac could be used as a monitoring or mass trapping tool forA. strojnymanagement in Chinese tea plantations. Furthermore, Z11-14:Ac was found to be a common attractant in the sex pheromones of nine leafroller species in the genusArchips. Thus, Z11-14:Ac may be used to develop a mating disruption technique targeting multiple leafroller species.AbstractPesticide application is the only known control method for the tea tortrixArchips strojny(Lepidoptera: Tortricidae), which is a major pest of spring tea in China. To develop sex pheromone-based, environmentally safe control strategies, here we identified the sex pheromone components of this species. The male moths’ antennae responded electrophysiologically to two compounds in female pheromone gland extracts. Gas chromatography–mass spectrometry analysis indicated that the two bioactive compounds were (Z)-11-tetradecenyl acetate (Z11-14:Ac) and (Z)-11-tetradecenyl alcohol (Z11-14:OH). Field trapping assays showed that lures baited with only the major component Z11-14:Ac were the most attractive to male moths, and the attractiveness decreased significantly when the lure was impregnated with increased relative ratios of the minor component Z11-14:OH. Our study demonstrated that Z11-14:Ac was the major attractant in theA. strojnysex pheromone, and the minor component Z11-14:OH seemed to serve as an antagonist. The results indicate that lures baited with 1 mg of Z11-14:Ac could be used as a monitoring or mass trapping tool forA. strojnymanagement in Chinese tea plantations. Furthermore, Z11-14:Ac was identified as a common sex pheromone attractant of nineArchipsspecies; these results lay the foundation for developing mating disruption techniques that target multiple leafroller pests.Keywords:female sex pheromone;Archips strojny;(Z)-11-tetradecenyl acetate;male attraction;tea pest
研究领域:
昆虫学
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农业科学
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化学生态学
✓ 已验证
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2024
·
IF 5.7
Haichun Zeng,Jiatong Zeng,Beilin Meng,Jianmei Peng,Li Rao
Geraniol, an acyclic monoterpenoid of substantial value extracted from the essential oils of various aromatic plants, holds significant commercial and industrial importance in the realms of food, cosmetics, medicine, and bioenergy. Geraniol synthase, which is responsible for geraniol production, has been identified in only several plant species to date. Here, we present the first cloning and characterization of a geraniol synthase (PgfTPS) from Penicillium griseofulvum. This enzyme demonstrates pronounced specificity in catalyzing the conversion of geranyl diphosphate into geraniol. Moreover, through protein modeling and site-directed mutagenesis, we have identified key active-site residues crucial for the catalytic function of PgfTPS. Finally, we utilized engineered Saccharomyces cerevisiae as a host for PgfTPS expression to facilitate geraniol production. Our findings not only advance the development of efficient biocatalysts for geraniol generation but also establish a fundamental basis for further exploration into fungal monoterpene biosynthesis.
研究领域:
生物化学
·
微生物学
·
植物代谢工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2025
·
IF 2.8
Xiuhong Duan,Zimeng Wang,Handa Liu,Hua Ma,Xuepeng Zhang,Lihua Liu,Hongzhou Shang,Ning Qiao
A predrug delivery system combining tumor-targeting and pH-responsive features was synthesized using baicalin (BAI), polyethylene glycol (PEG), and folic acid (FA). BAI and PEG were linked via esterification to create pH-sensitive ester bonds ( COO ) that facilitate drug release in acidic environments, while FA was attached to PEG through substitution. This system, named FA-PEG-COO-BAI (FPB), encapsulated curcumin (CUR) to form drug-loaded micelles. The impact of drug-loading conditions, such as sonication duration and initial drug dosage, on CUR/FA-PEG-COO-BAI encapsulation efficiency and drug release was investigated. In vitro studies demonstrated a maximum encapsulation rate of (73.09 ± 3.31)% and a drug-loading rate of (11.90 ± 0.69)%. Drug release from CUR/FA-PEG-COO-BAI micelles accelerated as pH decreased, confirming pH-responsive behavior. Cellular uptake and antitumor assays suggested effective tumor targeting and inhibition of proliferation. Hemolysis and cell viability assays indicated low toxicity. These findings underscore the potential of CUR/FA-PEG-COO-BAI micelles for controlled drug release and targeted therapy.
研究领域:
药剂学
·
肿瘤学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Chengshun Liu,Jianlong Li,Miao Wang,Guotai Jian,Chen Zhu,Hanxiang Li,Yongxia Jia,Jinchi Tang,Lanting Zeng
Camellia sinensis var. assamica is a major variety used to produce black tea. However, the core compounds influencing its aroma quality are unknown. In this study, Yinghong No. 9 black tea (YJBT), a distinctive black tea in South China, was analyzed. We detected 44 aroma compounds and identified seven key contributors, namely linalool, geraniol, trans -linalool oxide (furanoid), hexanal, methyl jasmonate, phenylacetaldehyde, and β-ionone. Linalool, which was generally more abundant in spring tea than in summer tea, contributed significantly to sweet and floral aromas. ( R )-Linalool was the primary linalool form in Yinghong No. 9, wherein it was highly abundant in high-quality tea leaves. The substantial expression of a linalool synthase gene and the relatively high catalytic efficiency of the encoded enzyme likely contributed to the accumulation of ( R )-linalool. This study identified the primary aroma compounds in YJBT, providing a foundation for advancing our knowledge of the aromatic quality of black tea.
研究领域:
植物学
·
食品科学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）