Results in Chemistry
·
2020
·
IF 0
Jiajia Fang,Beina Dong,Yingqiang Fu,Dingxing Tang
Thioglycolic acid-modified CuInS 2 quantum dots were prepared by a hydrothermal method. A method for the determination of sparfloxacin by a CuInS 2 ternary-quantum dot fluorescence probe was established based on the fact that the fluorescence of CuInS 2 quantum dots can be significantly quenched by sparfloxacin. The optimized experimental conditions are as follows: (1) the concentration of CuInS 2 quantum dots is 0.4 mol·L − 1 ; (2) the pH of the buffer solution is 6, and 1 mL of buffer was used; (3) the reaction time is 5 min. The concentration of sparfloxacin is linearly related to the fluorescence quenching intensity from 0.5 μg/mL to 1 mg/mL, and the maximum detection limit is 1 mg/mL.
研究领域:
分析化学
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纳米技术
·
药物分析
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DOI
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2019
·
IF 3.973
Kaiyu Wang,Yiteng Lei,Donglin Xia,Peipei Xu,Tao Zhu,Zhongying Jiang,Yuqiang Ma
Natural cellular membranes, with the outstanding qualities of biocompatibility and specificity, have gained growing attentions in the system of drug delivery. Nanoparticles coated with cellular membranes are starting to be applied as drug-loaded-vehicles to target tumors. Here, neutrophil membranes were selected to apply in the treatment of inflammation because neutrophils can participate in various inflammatory responses and accumulate at inflammatory sites to eliminate pathogens. Through extracting neutrophil membranes from natural neutrophils without affecting their biological properties, nanoparticles loaded with sparfloxacin (SPX) were coated with these membranes and disguised as neutrophils. Compared with traditional nano-medicines, the neutrophil membrane-coated nanoparticles (NM-NP-SPX) possessed precise targeting ability just like the neutrophils could accumulate at inflammatory sites when inflammation burst. In addition, NM-NP-SPX could prolong the circulation time and had the property of controlled-release. Through in vivo experiments, we found that the concentration of three representative inflammatory cytokines in blood, bacteria and inflammatory cells in lungs of the mice with pneumonia reduced significantly in the initial 24 h after the injection of NM-NP-SPX, which meant that NM-NP-SPX could greatly reduce the risk of death for the patients with inflammation. Moreover, the infected lungs could recover rapidly without any side effects to other organs due to the low cytotoxicity of NM-NP-SPX against normal cells. Therefore, our developed drug delivery system has enormous advantages in treating inflammations. Not only that, this kind of bionic method may have greater value and application prospects in curing the inflammations arisen from cancers.
研究领域:
药学
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纳米技术
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炎症研究
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药物递送系统
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Yuanyuan Geng,Manli Guo,Jiean Tan,Shuyi Huang,Youwen Tang,Lei Tan,Yong Liang
This paper describes an alternative strategy for the preparation of highly ordered molecularly imprinted mesoporous silica microspheres (MIMSM) for the selective fluorescence sensing of sparfloxacin in biological samples. We designed and synthesized a new functional monomer through the thiol-ene click chemistry reaction of 3-(methacryloyloxy) propyltrimethoxysilane and 3-mercaptopropionic acid. Sparfloxacin was selected as a template, and Mn-doped ZnS quantum dots served as the signal transducing element. This one-pot strategy was used to fabricate the MIMSM. Fourier transform infrared spectroscopy demonstrated successful embedding of a functional monomer in the mesoporous silica microspheres, and the highly ordered, periodic hexagonal mesoporous structure of MIMSM was confirmed with small angle X-ray diffraction, transmission electron microscopy, and N 2 adsorption-desorption measurements. The fluorescence intensity of the MIMSM had linear correlation with 0.05–2.0 μg/mL sparfloxacin. There was a rapid response time and excellent selectivity for structural analogues. Under optimal conditions, the limit of detection was 0.012 μg/mL for sparfloxacin. This method was then used with serum samples to achieve recoveries of 88.8–102%.
研究领域:
分析化学
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材料科学
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生物技术
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DOI
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2020
·
IF 7.727
Yang Yang,Yuan Ding,Bo Fan,Yi Wang,Zhengwei Mao,Weilin Wang,Jindan Wu
Sepsis is a complex disorder with very high morbidity and mortality; it can occur when an immune disorder triggers an invasion of pathogens in the host. Although many potential anti-infective and immunosuppressive treatments have been reported, we still do not have effective means of treating sepsis in clinic. The aim of this study is to develop a nanomaterial system that targets the site of inflammation and carries a combination of multiple drugs to better treat sepsis and alleviate its symptoms. We selected poly(lactide- co -glycolide acid) (PLGA) with good biocompatibility and degradability to prepare the nanoparticles (NPs) loaded with broad-spectrum antibiotic Sparfloxacin (SFX) and anti-inflammatory immunosuppressant Tacrolimus (TAC) by an emulsion-solvent evaporation method. The targeting ability of the NPs toward inflammatory sites is endowed by grafting of the γ3 peptide (NNQKIVNLKEKVAQLEA) that can specifically bind to the intercellular adhesion molecule-1 (ICAM-1), which is highly expressed on the surface of inflammatory endothelial cells. The drug loaded γ3-PLGA NPs have excellent cytocompatibility, low hemolysis ratio, and systemic toxicity. The drug loaded γ3-PLGA NPs also have excellent antibacterial property to both Gram-positive and Gram-negative bacteria and can effectively reduce the inflammation and immune response in acute lung infection mice. This study provides a simple and robust nanoplatform to treat lung infection induced sepsis, which may pave a way to design multifunctional nanomedicine for clinical translation.
研究领域:
药学
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纳米技术
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免疫学
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传染病学
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材料科学
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DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2024
·
IF 3.8
Tianci Lv,Fubin Liu,Mengqian Xiao,Ying Liu,Litao Wang,Guihua Gao
In this study, a novel hierarchical pore MIL-101(Cr) foam (HPF-MIL-101) was designed and prepared using the sacrificial template method with NaCl as the sacrificial template. This method involved grinding, heating, and washing the NaCl template to produce HPF-MIL-101, with PVDF as the binder and MIL-101(Cr) as the adsorbent. This preparation process is both straightforward and cost-effective, avoiding the use or generation of any organic reagents, thereby offering an environmentally sustainable approach for producing metal-organic framework (MOF) composites. The prepared HPF-MIL-101 exhibited excellent adsorption capabilities for both anionic dye (methyl orange, MO) and cationic dye (methylene blue, MB). The adsorption process followed a pseudo-second-order kinetic model and Friedrich isotherm model, indicating a multilayer adsorption. This is further supported by the Weber-Morris intraparticle diffusion model, which divided the adsorption process into three stages. Furthermore, the adsorption process was consistent with the Freundlich isotherm model, with a correlation coefficient ( r ) greater than 0.96. HPF-MIL-101 can also be used as an adsorbent for solid phase extraction (SPE). Therefore, an SPE method combined with high-performance liquid chromatography (HPLC) was developed using HPF-MIL-101 as the adsorbent to analyze five fluoroquinolones (FQs) in water samples. This analytical method showed good linearity in the range of 30–2000 ng·mL −1 , with excellent linear correlation coefficient ( r = 0.9991–0.9999), reasonable extraction recoveries ranging from 80.39 to 112.7 % (RSD ≤ 7.9 %), and low limits of detection (8–30 ng·mL −1 ). Overall, the results indicated that HPF-MIL-101 not only had a simple, environment-friendly, and pollution-free preparation process but also can be reused for enrichment and detection of trace FQs in water. Thus, HPF-MIL-101 exhibits immense application potential in environmental pollutant removal and also provides a valuable reference for the preparation and application of other MOF composites.
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Ruijie Tang,Lingfei Li,Yaowei Zhu,Minghui Zhan,Jun Zhao,Changlong Jiang,Bianhua Liu
Misuse of fluoroquinolone antibiotics (FQs) leads to environmental residues that threaten human health and ecosystems. Therefore, the development of efficient and sensitive micro-residue detection techniques for FQs is crucial for the environment and human health. Here, we developed a functionalised ratiometric fluorescence sensor for sensitive, rapid and intuitive detection and analysis of FQs residues in the environment. The sensor was constructed from green fluorescent carbon dots (GCDs) and red fluorescent europium metal–organic frameworks (Eu-MOFs). After the addition of FQs, the carboxyl group on the surface of FQs combined with the amino group on the surface of GCDs to form an amide bond, which was facilitated by the sensitisation effect and energy transfer, resulting in the fluorescence from green to red, and the detection limit of 4.3 nM, detection time within 1 s, which enables rapid micro-detection of FQs. Combined with 3D printing technology, we designed a hand-held fluorescent needle for direct quantification of FQs residues in real samples with a detection limit of 4.9 nM. This technology provides an innovative scientific approach to the rapid detection of trace levels of environmental contaminants, helping to reduce the health and environmental hazards of FQs.
研究领域:
环境科学
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分析化学
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生物医学工程
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 8
·1-12
·
IF 5.3
Huang Xiao-Qing,Wang Mao-Yu,Wang Xiao-Chen,Shi Xin,Li Rui-Wen,Wu Shuang,Li Ying
A surface-imprinted polymer SiO 2 @Eu(DBM) 3 phen/SMIP has been synthesized using enrofloxacin (ENFX) as the template molecule, methacrylic acid as the functional monomer, and the complex Eu(DBM)₃phen as a fluorescent moiety. The structure and the fluorescence sensing properties of SiO₂@Eu(DBM)₃phen/SMIP have been investigated in detail. The resulting material exhibited high selectivity, a low detection limit of 3.07 µM, and a linear working range from 20 to 100 µM for ENFX. The obtained fluorescence probe demonstrates good selectivity and sensitivity toward ENFX and has the potential for significant application in ENFX detection by simplifying procedures and reducing interference from impurities. In addition, the detection mechanism of the fluorescent probe was also discussed. The results indicate that it is feasible to use SiO 2 @Eu(DBM) 3 phen/SMIP for the detection of ENFX as well as its effective adsorption. This study explores the self-assembly of rare-earth complexes on silica via a surface imprinting technique, which provides a new solution for the subsequent development of the surface blotting technique. Graphical
研究领域:
分析化学
·
材料科学
·
荧光光谱学
·
表面印迹技术
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环境监测
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Shuai Han,Liang Yang,Zhigang Wen,Suyun Chu,Mei Wang,Zhenyang Wang,Changlong Jiang
Residues in animal food and drinking water caused by the abuse of antibiotics lead to cell resistance and many chronic diseases in the human body. Therefore, it has become an inevitable trend to develop a fast, easy-to-use, on-site/real-time visualization method for the detection of antibiotics. Herein, we report a dual-response ratiometric fluorescence sensor which is fabricated by chelating europium ions (Eu 3+ ) onto cadmium telluride quantum dots (CdTe QDs) for real-time and visible detection of tetracycline (TC). With the TC addition, the fluorescence of probe can be seen by the naked eye, from green to yellow and finally to red, exhibiting a dosage-sensitive and broad-chromatic detection strategy for TC. The fluorescence intensity ratio of I 616 /I 512 of Eu/CdTe QDs sensor displays a good linear relation to TC concentrations in the range of 0–80 μM with a limit of detection (LOD) of 2.2 nM. In addition, the sensor can visually detect 200 nM TC in actual samples, which is lower than the maximum residue limit (MRL) of the safety standard. The methodology reported here opens a window toward the real applications of fluorescent and shows the wide applicability in pursuing the concepts simple, rapid, visual, and real-time for food safety and environmental protection.
研究领域:
分析化学
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食品安全
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环境科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2020
·
IF 6.306
Kaili Yu,Mei-E. Yue,Jie Xu,Ting-Fu Jiang
A simple and sensitive salting out-assisted dispersive liquid-liquid microextraction method using deep eutectic solvent combined with back extraction and micellar electrokinetic capillary chromatography (SO-DLLME-DES-BE-MECC) was developed for the determination of fluoroquinolones in milk, honey and water samples. Several parameters affecting the extraction efficiency including DES volume, vortex time, centrifugation time, salt type and amount, sample pH and volume, etc. were investigated. Good linearity were obtained for fluoroquinolones in a range of 0.020–3.200 μg mL −1 and 0.030–4.800 μg mL −1 with LODs less than 0.010 μg mL −1 . The recoveries were in the ranges of 95.0–104.9%, 90.1–110.2% and 87.8–114.1% for water, honey and milk samples, respectively. The relative standard deviations for reproducibility were all below 7.6%. Under the optimized conditions, the enrichment factors for analytes were achieved in the range from 531 to 858 folds. The presented method was successfully applied for the determination of fluoroquinolones in milk, honey and water samples.
研究领域:
分析化学
·
食品科学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Qi Xue,Cong Wang,Yao Lin,Ting-Fu Jiang,Zhihua Lv
We optimized the combination of deep eutectic solvent (DES)-based ultrasonic-assisted dispersive liquid-liquid microextraction method and 19 F qNMR for quantitation of 5 kinds of fluoroquinolone antibiotics illegally added to drugs for prostatitis treatments (Ciprofloxacin, Levofloxacin, Sparfloxacin, Gatifloxacin, Enrofloxacin). In this study, methyltrioctylammonium bromide and n-capric acid were mixed at a 2:1 M ratio to prepare hydrophobic DES. The newly prepared DES was used as the extract. Fluoroquinolone antibiotics were extracted from saturated ammonium sulfate solution and detected by 19 F qNMR. In this study, the effects of extraction conditions such as DES volume, salt dosage, ultrasonic time, centrifugal time, and vortex time on extraction efficiency were systematically investigated. Under the optimal conditions, good linearity (correlation coefficient r: 0.9993–0.9998) was obtained with a detection limit of 0.1 μg mL −1 . The extraction reproducibility (RSD) of intra-day and inter-day were below 5.01 and 5.98%, respectively. This study showed feasibility of detection of fluoroquinolone antibiotics illegally added in traditional medicines for prostatitis treatments by DES-based ultrasonic-assisted dispersion-liquid microextraction combined with 19 F qNMR method.
研究领域:
分析化学
·
药学
·
医学检测
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产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2020
·
IF 7.489
Gongxi Qiao,Gui Chen,Qin Wen,Wanqiang Liu,Jinwei Gao,Zhiqiang Yu,Qianming Wang
The commercial production of carbon dots will be concerned with the simplicity and energy consumption. Herein, maleic acid and m-phenylenediamine form elegantly simple sources for carbon dots. The two precursors are dissolved in formamid (abbreviated as FA) or N, N -dimethylformamide (abbreviated as DMF) and the dehydration-condensation processes have been performed at 30 min or 120 min under room temperature. No external energy/irradiations, reactants or high temperature will be required and the afforded carbon dots (abbreviated as CDs) are collected by extraction, centrifugation, dialysis and column chromatography. It has been found for the first time the choice of organic solvents has been correlated with emission color. The blue-emitting CDs (abbreviated as B-CDs) and green-emitting CDs (abbreviated as G-CDs) are yielded in FA and DMF respectively. Facts support that the increase of –CONH- units causes red-shift in emissions. The optical sensing of tetracycline has been explored and the detection limit of blue-emitting CDs is as low as 25 nM. Live cells exposed to B-CDs and G-CDs (0.5 mg/ml) show no apparent changes via both Cell Counting Kit-8 and Annexin V/7-AAD analysis. The blue and green fluorescent signals can be easily tracked in cells. It has been demonstrated that the two carbon dots can be fabricated as multiple-color light-emitting diodes (abbreviated as LEDs).
研究领域:
材料科学
·
化学
·
纳米技术
·
光学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Yan-Fei Wang,Yang-Guang Wang,Xiao-Kun Ouyang,Li-Ye Yang
We herein describe a novel adsorbent based on molecularly imprinted polymers (MIPs) on the surface of magnetic carboxylated cellulose nanocrystals (Fe3O4@CCNs@MIPs) for the separation and purification of six fluoroquinolones (FQs) from egg samples. The obtained Fe3O4@CCNs@MIPs not only exhibited a large surface area and specific recognition toward FQs, but also were easily gathered and separated from the egg samples using an external magnetic field. The morphologies and surface groups of the Fe3O4@CCNs@MIPs were assessed by X-ray photoelectron spectroscopy, transmission electron microscopy, vibrating sample magnetometry, X-ray diffraction, thermogravimetric analysis, Fourier transform infrared spectroscopy, and Brunauer–Emmett–Teller surface area analysis. The Fe3O4@CCNs@MIPs exhibited high selectivity toward six structurally similar FQs. An enrichment approach was established for the measurement of six FQs from egg samples using Fe3O4@CCNs@MIPs coupled to high-performance liquid chromatography. The recovery of spiked FQs ranged from 75.2–104.9% and limit of detection was in the range of 3.6–18.4 ng g–1 for the six FQs. Therefore, the proposed method is a promising technique for the enrichment, separation, and determination of FQs from biomatrices.
研究领域:
分析化学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）