Marine Drugs
·
2019
·Vol. 17
·期 8
·438
·
IF 3.772
Xiaoyang Liu,Zixu Wang,Fawen Yin,Yuxin Liu,Ningbo Qin,Yoshimasa Nakamura,Fereidoon Shahidi,Chenxu Yu,Dayong Zhou,Beiwei Zhu
In this study, three synthetic zinc-chelating peptides (ZCPs) derived from sea cucumber hydrolysates with limited or none of the common metal-chelating amino-acid residues were analyzed by flame atomic absorption spectroscopy, circular dichroism spectroscopy, size exclusion chromatography, zeta-potential, Fourier transform infrared spectroscopy, Raman spectroscopy and nuclear magnetic resonance spectroscopy. The amount of zinc bound to the ZCPs reached maximum values with ZCP:zinc at 1:1, and it was not further increased by additional zinc presence. The secondary structures of ZCPs were slightly altered, whereas no formation of multimers was observed. Furthermore, zinc increased the zeta-potential value by neutralizing the negatively charged residues. Only free carboxyl in C-terminus of ZCPs was identified as the primary binding site of zinc. These results provide the theoretical foundation to understand the mechanism of zinc chelation by peptides.Keywords:zinc-chelating peptide;sea cucumber;chelation mechanism
研究领域:
生物化学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TREES-STRUCTURE AND FUNCTION
·
2021
·Vol. 36
·期 2
·711-721
·
IF 2.529
Xu Chenyi,Ma Yuandan,Tian Zhengfeng,Luo Qingyun,Zheng Tiefeng,Wang Bin,Zuo Zhaojiang
Key message The four chemotypes of adult C. camphora released different monoterpenes which protected these plants against high temperature by lowering ROS levels and maintaining photosynthetic abilities. Abstract High temperature seriously impacts plant growth and development, and terpenes are believed to improve plant thermotolerance through indoor experiments using seedlings. To uncover the actual protection effects of monoterpenes on outdoor adult plants, we investigated the monoterpene emissions, reactive oxygen species (ROS) metabolism, and photosynthetic abilities in four chemotypes of adult Cinnamomum camphora that were blocked monoterpene synthesis by fosmidomycin (Fos) under high temperature in summer (the mean highest temperature at about 38.2 °C). Eucalyptol (EuL), linalool (Lnl), borneol (BeL), and camphor (CmR) chemotypes of C. camphora released an abundance of monoterpenes, with eucalyptol, linalool, endo-borneol, and camphor as the typical monoterpene, respectively. When the four chemotypes of C. camphora were treated with Fos, their monoterpene emission was inhibited remarkably, with disappearance of some monoterpenes. In that case, the content of O 2 − , H 2 O 2 , and thiobarbituric acid reactive substance (TBARS) in the four chemotypes of C. camphora significantly increased although antioxidant enzyme activities remarkably increased, and the photosynthetic abilities significantly decreased by declining the quantum production and electron transport in photosystem II and increasing the dissipation of absorbed light energy. Among the four chemotypes, monoterpenes might play the maximum roles in CmR lowering ROS levels and in EuL maintaining photosynthesis. It can be speculated that monoterpenes can protect adult C. camphora against high temperature by lowering ROS levels and maintaining photosynthetic abilities.
研究领域:
植物生理学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMISTRY AND ECOLOGY
·
2022
·
IF 2.381
Yang Cai,Weijie Mu,Kun Jia,Di Huo,Yuhang Wang,Yawen Fan
Harmful effect of nanomaterials on aquatic organisms has accelerated in recent decades due to the growth emission of nanomaterials into water. In the present study, we analysed the toxic effects of TiO2, ZnO and MWCTN on diatoms Nitzschia palea in both individual and a combined way. After a short-term (96 h) exposure, the changes of cell density, chlorophyll a, MDA, SOD, CAT, total protein content and morphological structure were determined. Our results showed that: (1) The growth of N. palea was inhibited with the increase of concentration of nanomaterials; (2) The chlorophyll a content of N. palea was sensitive to these three individual nanomaterials; (3) Low concentration of nanomaterials promoted the MDA content in N. palea, but high concentration inhibited it; (4) The protein content of N. palea increased first and then decreased, indicating that nanomaterial stress inhibited diatom protein synthesis and showed dose-effect relationship; (5) After nanomaterial exposure, the SOD enzyme activity of N. palea was significantly inhibited, the CAT enzyme activity showed significantly decreased after exposure, comparing with control. The results of this study provide data for the study of algae nano-toxicology and provide a theoretical basis for the study of the toxicity of nanomaterials to freshwater microalgae.
研究领域:
环境科学
·
毒理学
✓ 已验证
DOI
高影响因子
MARINE ENVIRONMENTAL RESEARCH
·
2023
·
IF 3.3
Meng Cheng,Yong Luo,Xiao-Lei Yu,Lin-Tao Huang,Jian-Sheng Lian,Hui Huang
The co-occurrence of elevated seawater temperature and local stressors (heavy metal contamination) affects the ecophysiology of phototrophic species, and represents a risk to the environmental quality of coral reefs. Therefore, we investigated the effects of both Cu alone and Cu in combination with elevated temperature (ET) on the physiology of the coral Galaxea fascicularis , and measured the parameters related to the photo-physiology and oxidative state. G.fascicularis is one of the dominant coral species in the South China Sea which exhibits strong adaptability to environmental stress. We exposed the common coral species G.fascicularis to a series of environmentally relevant concentrations of Cu at 29 °C (normal temperature, NT) and 32 °C (elevated temperature, ET) for 96 h. Single polyps were used in the experiments, which reduced individual variability when compared to the coral colonies. The results suggested that: i) Cu or ET had significant negative effects on the actual operating ability of photosystem Ⅱ (PSII), but not on the maximal chlorophyll fluorescence in darkness (Fv/Fm). ii) Symbiodiniaceae density was significantly reduced by high Cu concentrations, for Cu-NT and Cu-ET, a high concentration of Cu (40 μg/L) significantly impacted Symbiodiniaceae density, causing a 75.4% and 81.0% decrease, respectively. iii) the content of malondialdehyde (MDA) in coral tissues increased significantly under Cu-ET. iv) a certain range of copper concentration (25–30 μg/L) increased the pigment content of the Symbiodiniacea. Our results indicated that the combined stressors of Cu and ET made the coral tissue sloughed, caused the coral tissue damaged by lipid oxidation, reduced the photosynthetic capacity of the Symbiodiniacea, and led to the excretion of Symbiodiniacea.
研究领域:
海洋生物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Environmental Science-Processes & Impacts
·
2024
·
IF 4.3
Chang Xu,Bo Gong,Shan Zhao,Xiao-Min Sun,Shu-Guang Wang,Chao Song
Tetracycline (TC) and Cu(II) coexist commonly in various waters, which may infiltrate into the subterranean environment through runoff and leaching, resulting in substantial ecological risks. However, the underlying mechanisms why Cu(II) affects the transport of TC in porous media remain to be further explored and supported by more evidence, especially the role of complexation. In this study, the transport of TC with coexisting Cu(II) was comprehensively explored with column experiments and density functional theory (DFT) calculation. At natural environmental concentrations, Cu(II) significantly inhibited the transport of TC in the quartz sand column. Cu(II) augmented the retention of TC in the column mainly via electrostatic force and complexation. The interaction between TC and TC–Cu complexes on the surface of SiO2 was investigated with first-principles calculations for the first time. There were strong van der Waals forces and coordination bonds on the surface of complexes and SiO2, leading to higher adsorption energy than that of TC and inhibiting its penetration. This study offers novel insights and theoretical framework for the transport of antibiotics in the presence of metal ions to better understand the fate of antibiotics in nature.
研究领域:
环境科学
·
理论化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROPHORESIS
·
2024
·
IF 3
Deyu Li,Rui Jiang,Jun Li,Zhen Liu,Yasmeen Zamir Ahmed,Qiankun Zhao,Yongxin Song,Mengqi Li
The viability detection of microalgae with the electrokinetic (EK) technique shows vast applications in the biology and maritime industry. However, due to the slight variations in the EK properties between alive and dead microalgae cells, the accuracy and practicability of this technique is limited. In this paper, the light illumination pretreatment was conducted to modify the EK velocity of microalgae for enhancing the EK difference. The effects of the illumination time and light color on the EK velocities of Chlorella vulgaris and Isochrysis galbana were systematically measured, and the EK differences between alive and dead cells were calculated and compared. The results indicate that under light illumination, the photosynthesis of the alive cells leads to the amplification of the zeta potential, leading toward increase in the EK difference along with the illumination time. By using light with different color spectra to treat the microalgae, it was found that the EK difference changes with the light color according to the following order: white light > red light > blue light > green light. The difference in EK potential with exposure to white light treatment surpasses over 10-fold in comparison to those without such treatment. The light pretreatment technique, as illustrated in this study, offers an advantageous strategy to enhance the EK difference between living and dead cells, proving beneficial in the field of microalgae biotechnology.
研究领域:
生物技术
·
海洋科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Microbiology
·
2025
·
IF 4.5
Ting-Bin Hao,Peng-Yu Lai,Zhan Shu,Ran Liang,Zhi-Yun Chen,Ren-Long Huang,Yang Lu,Adili Alimujiang
Water scarcity is an escalating environmental concern. The model diatom, Phaeodactylum tricornutum, holds promise as a potential cell factory for the production of high-value natural compounds. However, its dependence on saline water cultivation restricts its use in areas facing water shortages. Although numerous studies have delved into the metabolic mechanisms of plants under water stress, there is a limited understanding when it comes to microalgae. In our study, we employed polyethylene glycol (PEG) to simulate water scarcity conditions, and assessed a range of parameters to elucidate the metabolic responses of P. tricornutum. Water stress induced the generation of reactive oxygen species (ROS), curtailed the photosynthetic growth rate, and amplified lipid content. Our insights shed light on the physiology of P. tricornutum when subjected to water stress, setting the stage for potential applications of microalgae biotechnology in regions grappling with water scarcity.
研究领域:
藻类生物学
·
代谢组学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACTA PHYSIOLOGIAE PLANTARUM
·
2016
·Vol. 39
·期 1
·1-14
·
IF 1.563
Wang Yang,Ren Tao,Lu Jianwei,Cong Rihuan,Hou Wenfeng,Liu Tao,Hussain Saddam,Li Xiaokun
Inferior tillers typically provide a minimal contribution to the grain yields of rice; however, with the proper regulation of light and nutrients, their yields may be significantly enhanced. The exogenous introduction of gibberellic acid (GA 3 ) may assist with extending the culm lengths of inferior tillers, the sequestration of additional sunlight, and the accumulation of further nutrients. In an attempt to verify our assumption while enhancing the grain yield of inferior tillers, GA 3 was applied to inferior tillers under different nitrogen (N) levels. Morphological alterations, photosynthetic parameters, endogenous GA content, N accumulation, and soluble sugars within the leaves of inferior tillers were assessed, as was the activity of sucrose synthase (SuSase) in the cleavage direction. Our results revealed that the belated emergence and low concentration of endogenous GA 1+3 translated to the stunted growth of inferior tillers, which ultimately had a reduced capacity to harvest sunlight. Furthermore, insufficient sunlight due to asymmetric competition might be the source of the reduction of inferior tiller source and sink activities. Foliar GA 3 spray enhanced the intercepted photosynthetically active radiation (IPAR) and SuSase activity in inferior tillers considerably; however, dry matter and N accumulation provided adequate resources to grains only when the tillers obtained sufficient N. The alterations in soluble sugars indicated that inferior tillers did not respond well to the application of GA 3 under an insufficient N supply. In contrast, when sufficient N was made available, GA 3 -treated inferior tillers received high-intensity light, and additional energy was allocated to the photochemical reactions of photosystem II (PSII). The high quantum efficiency of PSII contributed to the transportation of C- and N-assimilates from the source organ to the sink under adequate N conditions, and eventually increased the grain yields of the inferior tillers.
研究领域:
植物生理学
·
农学
✓ 已验证
产品关联
DOI
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2021
·
IF 4.401
Pingkang Qian,Kun Gao,Zheng Gao,Chunye Xue,Xiaoli Hu,Qiaoqiao Liu,Jingwei Dong,Xiangyuan Deng
The aim of this work was to investigate the effects of myo -inositol (MI) on growth, photosynthetic activity , and chemical compositions of Chlorella sorokiniana . Results showed that a maximum value of cell density was obtained when this alga was cultivated in BG11 media with 1000 mg L −1 MI, which was 1.37 times greater than that in the controls. Moreover, it was observed that MI had a negative effect on algal photosynthetic activity, which could be alleviated by regulating the contents of chemical compositions, such as chlorophyll a (Chl a ). In the BG11 media with MI, contents of Chl a in the algal cells were in the range of 0.30–0.35 μg 10 −6 cells, which were about 1.75 times greater than that in the controls during microalgae cultivation. The accumulated Chl a would be used to capture and deliver more photons during algal photosynthesis . In addition, contents of lipids , carbohydrates, and proteins varied with the changes in MI concentrations. Thus, it is concluded that MI could promote the algal growth and play an important role in regulating the biochemical compositions in algal cells. These data would provide basic theory and technical support for large-scale cultivation and biomass production of microalgae by adding MI to culture media.
研究领域:
水生生物学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-11
·
IF 3.8
Chen Hongxing,Huang Xiaoyun,Chen Hui,Zhang Song,Fan Chengwu,Fu Tianling,He Tengbing,Gao Zhenran
Cadmium (Cd) pollution is a serious threat to food safety and human health. Minimizing Cd uptake and enhancing Cd tolerance in plants are vital to improve crop yield and reduce hazardous effects to humans. In this study, we designed three Cd concentration stress treatments (Cd1: 0.20 mg·kg −1 , Cd2: 0.60 mg·kg −1 , and Cd3: 1.60 mg·kg −1 ) and two foliar silicon (Si) treatments (CK: no spraying of any material, and Si: foliar Si spraying) to conduct pot experiments on soil Cd stress. The results showed that spraying Si on the leaves reduced the Cd content in brown rice by 4.79–42.14%. Si application increased net photosynthetic rate (Pn) by 1.77–4.08%, stomatal conductance (Gs) by 5.27–23.43%, transpiration rate (Tr) by 2.99–20.50% and intercellular carbon dioxide (CO 2 ) concentration (Ci) by 6.55–8.84%. Foliar spraying of Si significantly increased the activities of superoxide dismutase (SOD) and peroxidase (POD) in rice leaves by 9.84–14.09% and 4.69–53.09%, respectively, and reduced the content of malondialdehyde (MDA) by 7.83–48.72%. In summary, foliar Si spraying protects the photosynthesis and antioxidant system of rice canopy leaves, and is an effective method to reduce the Cd content in brown rice.
研究领域:
植物学
·
环境科学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Sustainability
·
2022
·Vol. 14
·期 5
·2614
·
IF 3.889
Jieming Zhu,Chen Chen,Lie You
Serious farmland scarcities make smallholders a default mode for China’s agriculture, which makes efficient and equitable rural development a great challenge. Tensions lead to alternation between autonomous family farming and coordinated collective agriculture. Rapid urbanization since the 1980s has strongly stimulated flower-growing and agribusiness in Yunnan, China. The organization for commercial flower-farming is, however, an issue. Officially promoted for collective farming, voluntary cooperatives are wrecked by the free-riding problem. Grower associations nevertheless spontaneously emerge, with the flexible entry and exit of members without binding joint-assets and joint-ownership, which is facilitated by technological changes to the transaction. Empirical investigation in Tonghai, Yunnan, unveiled the institution of agribusiness–smallholder partnership for inclusive rural development. Smallholders have actively participated in flower agriculture, which has contributed significantly to the development of rural economies. The high casualty of micro-smallholders suggests that farm size is an important and crucial factor for sustainable farming. Effective rural development has to be supported by endogenous non-agricultural jobs so that farm size can be increased.
研究领域:
农业经济学
·
社会学
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2025
·
IF 7.7
Zeliang Liu,Xuanlin Cao,Manli Wu,Wenjie Huang,Xia Dong,Xing Chen,Chun Zhang
Perfluorobutanoic acid (PFBA), an emerging alternative to perfluorooctanoic acid (PFOA), has become increasingly prevalent in aquatic ecosystems, yet its ecotoxicological impacts remain poorly understood. This study investigated the aquatic toxicity of PFBA using the freshwater algae Chlorella vulgaris ( C . vulgaris ) as a model organism, employing a 96h pre-exposure assay to determine the median effective concentration followed by acute toxicity experiments analyzing multiple endpoints including growth, photosynthetic parameters, oxidative stress markers, and antioxidant enzyme activities. Computer simulation techniques were utilized to illustrate the underlying molecular mechanisms of PFBA toxicity. The results showed that the 96h-EC 50 value of PFBA was 154.88 mg/L, which is comparable to conventional per- and polyfluoroalkyl substances (PFAS). Acute toxicity experiments revealed a biphasic dose-response relationship to the algal growth with the hormetic effects at the lower concentrations (30.97–92.93 mg/L) but inhibition at the higher levels (123.91–185.86 mg/L) of PFBA. High dosages of PFBA significantly decreased the maximum photosynthetic yield (Fv/Fm) and relative electron transfer rate (rETR), while inducing oxidative stress and inhibiting superoxide dismutase (SOD) and catalase (CAT) activities. Future AlphaFold2 modeling and molecular docking simulations demonstrated the potential binding of PFBA to photosystem II D1 C-terminal processing protease (PSII D1 protein), SOD, and CAT. These findings reveal a complex toxicity mechanism of PFBA on C . vulgaris involving photosynthetic disruption, oxidative stress, and antioxidant system impairment, contributing to the understanding of short-chain PFAS alternative ecotoxicity in aquatic ecosystems.
研究领域:
环境科学
·
毒理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）