Forensic Toxicology
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2018
·Vol. 37
·期 1
·121-131
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IF 3.924
Chen Lizhu,Yu Yingjia,Wang Yang,Xiang Ping,Duan Gengli
Purpose It is crucial for forensic analysis to differentiate clinical use from illegal abuse. Selegiline (SG) is mainly metabolized to desmethylselegiline (DM-SG), R -(−)-methamphetamine (R-MA) and R-(−)amphetamine (R-AM); while abused methamphetamine and amphetamine mainly contain the S -(+)-form. The aim of this study was to simultaneously determine SG, DM-SG, R / S -MA, and R / S -AM in human oral fluid (OF) and to differentiate clinical use from illegal use. We also aim to apply the present method to the OF samples from authentic cases in forensic toxicology. Methods Liquid–liquid extraction and liquid chromatography-tandem mass spectrometry operating in positive ion multiple-reaction monitoring mode were utilized. The chromatographic system consisted of a Chirobiotic TM V2 column (2.1 mm × 250 mm, 5 μm) and a mobile phase of methanol containing 0.1% (v/v) glacial acetic acid and 0.02% (v/v) ammonium hydroxide. Results and conclusions The calibration curves were linear from 1 to 100 ng/mL, and r > 0.995 for all analytes with imprecisions ≤ 15% and accuracy between 85 and 115%. Extraction recoveries ranged from 46.3 to 104.7% with coefficient of variation (CV) ≤ 10.3% and matrix effects (MEs) ranged from 47.4 to 114.8% with CV ≤ 12.9%. The lower limit of quantification was 0.2 ng/mL for SG and DM-SG and 1.0 ng/mL for S-MA, R-MA, S-AM and R-AM. The present method is simple, rapid (accomplished in 12 min), sensitive, and validated by authentic samples.
研究领域:
法医学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Chemistry
·
2019
·
IF 3.782
Chen Lizhu,Yu Yingjia,Duan Gengli,Wang Xin,Shen Baohua,Xiang Ping
Objective: Chiral analysis is a crucial method to differentiate selegiline intake from drug abuse. A dried urine spot (DUS) analytical method based on spotting urine samples (10 μL) onto dried spot collection cards, and followed by air-drying and extraction, was developed and validated for the determination of selegiline, desmethylselegiline, R/S-methamphetamine, and R/S-amphetamine.Methods: Methanol (0.5 mL) was found to be the ideal extraction solvent for target extraction from DUSs under orbital-horizontal stirring on a lateral shaker at 1,450 rpm for 30 min. Determinations were performed by direct electrospray ionization tandem mass spectrometry (ESI-MS/MS) under positive electrospray ionization conditions using multiple reaction monitoring mode. The chromatographic system consisted of a ChirobioticTM V2 column (2.1 × 250 mm, 5 μm) and a mobile phase of methanol containing 0.1% (v/v) glacial acetic acid and 0.02% (v/v) ammonium hydroxide.Results and conclusions: The calibration curves were linear from 50 to 5,000 ng/mL, with r > 0.995 for all analytes, imprecisions ≤ 15% and accuracies between −11.4 and 11.7%. Extraction recoveries ranged from 48.6 to 105.4% with coefficients of variation (CV) ≤ 13.7%, and matrix effects ranged from 45.4 to 104.1% with CV ≤ 10.3%. The lower limit of quantification was 50 ng/mL for each analyte. The present method is simple, rapid (accomplished in 12 min), sensitive, and validated by a pharmacokinetic study in human urine collected after a single oral administration of SG.
研究领域:
分析化学
·
药理学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD PROPERTIES
·
2020
·
IF 1.808
Di Hu,Mengmeng Jiang,Ting Ge,Xu Liu,Ziqi Li,Jinhong Liu,Ketong Zhu
A study of pesticide residues was investigated in vegetable samples to highlight the trend of drugs obtained from the Jilin Province, China. Total of 230 samples obtained from four regions over the period 2016–2017 were screened for 18 pesticides that were analyzed using gas chromatography with electron capture (GC-ECD). The results showed that 45 samples (19.6%) were positive including 17 samples (7.39%) exceeded the national maximum residue limit (MRL). Pesticides positive samples were detected in 32 leafy vegetables, 14 of which exceeded the MRLs (6.07%). There are 12 samples detected in celery that its detection rate was 5.22%, five of which exceeded the MRLs at the same time. In contrast, no pesticide residues were detected in beans or cauliflower. In the four types of pesticides, organophosphorus pesticides had the highest detection rate, which was 12.6% (29 samples). On the other hand, detectable residues of organochlorine pesticides were found, but it did not exceed the standard. Regional variations in pesticide occurrence were observed: fifteen, seven, and ten samples were positive from Ji’an, Siping City, and Tonghua City, respectively, six samples from the last city exceeded MRLs. There were 30 samples tested for pesticides at the farmers’ market and its detection rate was 13.04%, while only 15 samples contain pesticides that the samples (6.5%) purchased in the supermarket. The results indicated that high levels of the occurrence of pesticide residues in vegetables was an important source of exposure to pesticides. Therefore, we should pay attention to the consumers and their impact of pesticide residues on food and human health should be considered.
研究领域:
食品安全
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环境科学
·
公共卫生
✓ 已验证
DOI
高影响因子
JOURNAL OF FOOD PROTECTION
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2019
·
IF 1.559
Xinxin Liu,Liwu Wang,Hao Li,Juxin Yin,Shaowu Lv,Ketong Zhu
ABSTRACT The purpose of this study was to investigate the status of organochlorine pesticide residues in 186 ginseng samples collected in Jilin, People's Republic of China. Based on the Chinese Pharmacopoeia 2015 method for detection of organochlorine pesticide residues in ginseng, 22 organochlorine pesticide residues were identified. Chlordane, aldrin, epichlorohydrin, and dieldrin and their isomers were not detected in ginseng from this region. Heptachlor was detected in only one ginseng sample, and the concentration did not exceed the maximum residual limit (MRL) prescribed in the Pharmacopoeia (0.05 mg/kg). Benzene hexachloride was detected in two samples, one of which was above the MRL. Hexachlorobenzene and pentachloronitrobenzene (quintozene) were found in 11.8 and 52.1% of the samples, respectively, and the residues in these samples exceeded the MRL by 4.3 and 8.6%, respectively. HIGHLIGHTS Eight organochlorine pesticides and their isomers were detected in ginseng from Jilin, China. The most common organochlorine pesticide, quintozene, was detected in 52.15% of the samples. Only 9% of ginseng samples contained organochlorine pesticides above the MRL.
研究领域:
药学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Xiaodong Guo,Jundan Yi,Zhuqing Wan,Xin Wang,Zhiruo Yuan,Waisze Cheung,Xiaoqiang Bai,Yang Fu,Mo Zhai,Longwei Lv,Yongsheng Zhou
4D-printing offers great potentials for fabricating scaffolds with dynamic shapes and functions, which are desirable for the repair of complex bone defects (such as those in oral and maxillofacial region). However, most current 4D-printed scaffolds are far from ideal for clinical applications due to their unsuitable deformation conditions, inherent hydrophobicity, and lack of bone regenerative function. The design of a morphology and function dual-adjustable 4D-printed scaffold with biomimetic “hard-soft” compositions is proposed. The “hard” 4D-printed framework, fabricated from the composite of poly (l-lactide-co-trimethylene carbonate) (PLMC) and nano-hydroxyapatite (nHA) via extrusion-based 3D printing, is demonstrated to possess suitable transition temperature (T trans ) slightly above the physiological level, along with favorable mechanical and shape memory properties. Furthermore, the “soft” hydrogel layer containing nHA@polydopamine (PDA) nanoparticles loaded with pargyline (PGL) (nHA@PDA-PGL) is integrated with the framework to enhance hydrophilicity and cytocompatibility, as well as to offer near-infrared (NIR) light-responsive photothermal effect and sustained release of PGL to promote osteogenesis. This composite system exhibited unique NIR light-triggered shape recovery capability, morphological adaptation to the defects, along with significantly improved bone regeneration efficacy. Overall, these findings underscore the remarkable potential of the present 4D-printed scaffold for the regenerative restoration of complex bone defects.
研究领域:
生物材料学
·
组织工程
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口腔颌面外科学
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再生医学
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生物医学工程
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高分子科学
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纳米医学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）