MOLECULES
·
2018
·Vol. 23
·期 12
·3214
·
IF 3.098
Jun Cao,Huimin Wang,Wei Zhang,Fuliang Cao,Geli Ma,Erzheng Su
Ginkgo bilobaleaves have various health benefits due to the presence of bioactive compounds such as polyprenyl acetates, flavonoids, and terpene trilactones. However, there is little literature reported on the aromatic acids inGinkgo bilobaleaves. In this work, five aromatic acids including shikimic acid (SA), 6-hydroxykynurenic acid (6-HKA), protocatechuic acid (PA), gallic acid (GAA), andp-hydroxybenzoic acid (PHBA) were simultaneously extracted fromGinkgo bilobaleaves by employing the green deep eutectic solvents (DESs). A DES tailor-made from xylitol, glycolic acid and 1,5-pentanedioic acid at a molar ratio of 1:3:1 with 50% (w/w) water addition, named as NGG50, gave higher extraction yields for the five aromatic acids. Main factors affecting the extraction process were further optimized. The highest extraction yields of SA, GAA, 6-HKA, PA, and PHBA were 94.15 ± 0.96 mg/g, 332.69 ± 5.19 μg/g, 25.90 ± 0.61 μg/g, 429.89 ± 11.47 μg/g and 67.94 ± 0.37 μg/g, respectively. The NGG50-based extraction process developed here was a successful attempt of simultaneously extracting five aromatic acids fromGinkgo bilobaleaves for the first time, which could provide a new exploitation direction ofGinkgo bilobaleaves.Keywords:deep eutectic solvents;Ginkgo bilobaleaves;simultaneous extraction;shikimic acids;6-hydroxykynurenic acid
研究领域:
药学化学
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分析化学
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植物科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Di Chen,Jing-Xian Zhang,Wei-Qi Cui,Jun-Wei Zhang,De-Qiao Wu,Xin-Rui Yu,Yan-Bo Luo,Xing-Yi Jiang,Feng-Peng Zhu,Dilshad Hussain,Xia Xu
The current study presents a convenient, rapid and effective simultaneous extraction/derivatization (SEDP) strategy for effective pretreatment of catecholamines (CAs). Commercial zirconium oxide (ZrO 2 ) nanoparticles were employed for the selective capturing of cis-diol containing CAs to remove the biological interferences and phenyl isothiocyanate (PITC) was used for derivatization to improve the ionization and to improve the chromatographic separation. The extraction and derivatization procedures were integrated into one step to simplify the sample pretreatment. Excessive derivatization reagents were removed as well, reducing the degree of contaminations in mass spectrometry. The factors affecting the SEDP process were optimized and the results showed that the detection sensitivity and chromatographic separation of CAs greatly improved compared with underivatized CAs, during LC-MS/MS analysis. Combined with ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), quantifying the concentration of norepinephrine (NE), epinephrine (E) and dopamine (DA) in biological fluids was validated in ranges of 1-200.0 ng/mL with a satisfactory correlation coefficient (R 2 > 0.997). The obtained recoveries were in the range of 91.0-109.5% with RSDs less than 9.4%. Finally, significant changes in CAs levels in urine samples of healthy people and pheochromocytoma patients were detected. The developed method offers comparative advantages in terms of sensitivity, specificity and selectivity.
研究领域:
分析化学
·
医学检验
·
药物分析
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DOI
PubMed
高影响因子
MOLECULES
·
2021
·Vol. 26
·期 19
·6030
·
IF 4.412
Zhanglan He,Kangzhuo Yang,Zhipeng Liu,Mingzhe An,Zongwei Qiao,Dong Zhao,Jia Zheng,Michael C. Qian
Wuliangye baijiu is one of the most famous baijiu in China, with a rich, harmonic aroma profile highly appreciated by consumers. Thousands of volatiles have been identified for the unique aroma profile. Among them, fatty acid esters have been identified as the main contributors to the aroma profile. In addition, many non-ester minor compounds, many of which are more polar than the esters, have been identified to contribute to the characteristic aroma unique to Wuliangye baijiu. The analysis of these minor compounds has been challenging due to the dominance of esters in the sample. Thus, it is desirable to fractionate the aroma extract into subgroups based on functional group or polarity to simplify the analysis. This study attempts a new approach to achieve simultaneous volatile extraction and fractionation using tandem LiChrolut EN and silica gel solid-phase extraction (SPE) columns. A baijiu sample (10 mL, diluted in 40 mL of water) was first passed through the LiChrolut EN (1.0 g) column. The loaded LiChrolut EN column was then dried with air and coupled with a silica gel (5.0 g) SPE column with anhydrous Na2SO4(10.0 g) in between. The volatile compounds were eluted from the LiChrolut EN column and simultaneously fractionated on the silica gel column based on polarity. The simultaneous extraction and fractionation technique enabled the fractionations of all fatty acid esters into less polar fractions. Fatty acids, alcohols, pyrazines, furans, phenols, hydroxy esters, and other polar compounds were collected in more polar fractions. This technique was used to study the volatile compounds in Wuliangye, Moutai, and Fengjiu baijiu. In addition to fatty acid esters, many minor polar compounds, including 2,6-dimethylpyrazine, 2-ethyl-6-methylpyrazine, 2-ethyl-3,5-dimethylpyrazine,p-cresol, and 2-acetylpyrrole, were unequivocally identified in the samples. The procedure is fast and straightforward, with low solvent consumption.Keywords:Wuliangye baijiu;simultaneous extraction and fractionation;tandem SPE columns;fatty acid esters;polar compounds
研究领域:
食品科学
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分析化学
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风味化学
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产品关联
DOI
PubMed
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Xinyu Yang,Tingting Liu,Mengxia Wei,Ru Zhao,Huiyan Gu,Jialei Li,Fengli Chen,Lei Yang
An improved approach was developed to obtain essential oil, rosmarinic acid , and polysaccharides with sucrose stearate as a green additive from Perilla frutescens (L.) Britt. Leaves using microwave-induced hydrodistillation and simultaneous extraction (MHDE). In this process, we realize the rotation of the material through a rotary motor so that the material is uniformly irradiated by microwaves. The influencing factors on MHDE were systematically optimized by single factor and central composite design. Under the obtained optimization conditions, the yields of essential oil, rosmarinic acid, and polysaccharides were 0.64% ± 0.03%, 0.18% ± 0.01%, and 4.35% ± 0.19%, respectively. GC-MS analysis results showed that the main component of essential oil prepared by MHDE was perillaldehyde , which accounted for 51.91% of the total essential oil, followed by caryophyllene , accounting for 15.79%. However, the ratio of perillaldehyde in the essential oil obtained by traditional hydrodistillation and extraction (HDE) was only 40.69%, and the proportion of caryophyllene was only 12.50%. MHDE uses sucrose stearate with a long hydrocarbon chain structure as an additive, which reduces the nonterpene aliphatics in the obtained essential oil.
研究领域:
化学工程
·
植物化学
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食品科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）