NANOTECHNOLOGY
·
2014
·Vol. 25
·期 41
·415501
·
IF 3.672
Zhao Sheng Qian,Xiao Yue Shan,Lu Jing Chai,Jian Rong Chen,Hui Feng
Convenient and simultaneous detection of multiple biomarkers such as DNA and proteins with biocompatible materials and good analytical performance still remains a challenge. Herein, we report the respective and simultaneous detection of DNA and bovine α-thrombin (thrombin) entirely based on biocompatible carbon materials through a specially designed fluorescence on-off-on process. Colorful fluorescence, high emission efficiency, good photostability and excellent compatibility enables graphene quantum dots (GQDs) as the best choice for fluorophores in bioprobes, and thus two-colored GQDs as labeling fluorophores were chemically bonded with specific oligonucleotide sequence and aptamer to prepare two probes targeting the DNA and thrombin, respectively. Each probe can be assembled on the graphene oxide (GO) platform spontaneously by π–π stacking and electrostatic attraction; as a result, fast electron transfer in the assembly efficiently quenches the fluorescence of probe. The presence of DNA or thrombin can trigger the self-recognition between capturing a nucleotide sequence and its target DNA or between thrombin and its aptamer due to their specific hybridization and duplex DNA structures or the formation of apatamer–substrate complex, which is taken advantage of in order to achieve a separate quantitative analysis of DNA and thrombin. A dual-functional biosensor for simultaneous detection of DNA and thrombin was also constructed by self-assembly of two probes with distinct colors and GO platform, and was further evaluated with the presence of various concentrations of DNA and thrombin. Both biosensors serving as a general detection model for multiple species exhibit outstanding analytical performance, and are expected to be applied in vivo because of the excellent biocompatibility of their used materials.
研究领域:
生物化学
·
纳米医学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Electrochemical Science
·
2023
·
IF 1.5
Mengting Zhu,Ruli Xu,Xueyang Wang,Jie Liu Qianli Zhang,Jie Wei
Four Fe-based metal-organic frameworks (MIL-101(Fe), MIL-53(Fe), MIL-88(Fe), and MIL-100(Fe)) were synthesized using a solvothermal method, and further characterized by X-ray diffraction, Fourier transforms infrared spectroscopy, Scanning electron microscopy, and X-ray photoelectron spectroscopy. The synthesized Fe-MOFs were used to fabricate the modified carbon paste electrodes (CPE) for simultaneous detection of hydroquinone and catechol. Among the MILs (Fe) modified CPEs, MIL-101(Fe)/CPE exhibited extraordinary electrochemical sensitivity because of MIL-101 (Fe)’s excellent conductivity. The oxidation potential separation of hydroquinone and catechol achieved 190 mV. Compared with CPE, the oxidation currents of hydroquinone and catechol at MIL-101/CPE were 10-fold higher and the reduction currents were 5-fold higher. The linear range was 2 - 90 μM for hydroquinone and 2 - 100 μM for catechol, and the detection limit was 0.58 μM and 0.62 (S/N = 3) μM for hydroquinone and catechol. Furthermore, the application of MIL-101 (Fe)/CPE has been validated in an actual river water sample with the satisfied recoveries of 90.21 - 102.36% and 91.11 - 102.19% for hydroquinone and catechol, respectively.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYST
·
2021
·Vol. 146
·期 24
·7593-7600
·
IF 4.616
Ling Lei,Caoling Li,Wensheng Huang,Kangbing Wu
Developing a sensitive and rapid detection method for 4-chlorophenol (4-CP) and 4-nitrophenol (4-NP) is very important due to their high toxicity. In this work, bulk Ti3AlC2 powder was etched to Ti3C2Tx for the first time through a hydrothermal reaction in NaF/HCl solution. After ultrasonication in N-methylpyrrolidone (NMP), Ti3C2Tx powder was successfully exfoliated into multilayered Ti3C2Tx nanosheets (i.e. Ti3C2Tx MXene). The prepared Ti3C2Tx MXene not only has a large electrochemical surface area for the oxidation of 4-CP and 4-NP, but also lowers their electron transfer resistance. As a result, the oxidation signals of 4-CP and 4-NP are significantly improved on the surface of the Ti3C2Tx MXene. Based on the remarkable signal amplification of the Ti3C2Tx MXene, a sensitive and rapid method was developed for the simultaneous detection of 4-CP and 4-NP. The linear range is from 0.1 to 20.0 μM for 4-CP, and from 0.5 to 25.0 μM for 4-NP, with detection limits of 0.062 μM (4-CP) and 0.11 μM (4-NP). This method was used in wastewater samples, and the accuracy was confirmed to be good by high-performance liquid chromatography.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2014
·
IF 3.259
Meiling Wang,Yunqiao Gao,Qian Sun,Jianwei Zhao
In the present work, a novel strategy based on new carbon nanotube (CNT) and polypyrrole (ppy) composite modified electrode for the simultaneous determination of the isomers of Amaranth (E123) and Ponceau 4R (E124) was proposed. Due to the increasing dispersibility and effective surface area of the CNT caused by decoration of ppy to the CNT, the proposed electrode exhibited excellent electrochemical sensitivity and high selectivity toward the oxidation of Amaranth and Ponceau 4R. The linear response ranges for Amaranth and Ponceau 4R were 5.0 × 10 −9 M–5.0 × 10 −7 M, and 8 × 10 −9 M–1 × 10 −6 M and corresponding detection limits as low as 5.0 × 10 −10 M and 1.0 × 10 −9 M, respectively. This proposed method was successfully applied in the detection of Ponceau 4R and Amaranth in fruit drink samples.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2013
·
IF 3.535
Wen Zhang,Yaqin Chai,Ruo Yuan,Jing Han,Shihong Chen
This work described a two-step conjugate synthesis of a porous nanostructured materials (PTCA–Cys) composed of 3,4,9,10-perylenetetracarboxylic acid (PTCA) and l -cysteine (Cys) and then the sensor prepared by the deposited gold nanocrystals (DpAu) immobilized in this nanostructure film was developed. The interesting PTCA–Cys layer not only could provide SH-functionalized interface but also easily forms a stable thin film on the electrode surface with the porous nanostructure , thus, it is very beneficial to electrode modification. Furthermore, with the DpAu film onto PTCA–Cys modified electrode , the proposed sensors exhibited the excellent catalytic activity and the high selectivity toward the oxidation of AA , DA and UA . Moreover, scanning electron microscopy, cyclic voltammetry and different pulse voltammetry were employed to characterize the sensors. Under the optimal conditions, the linear response range for simultaneous detection of AA, DA, and UA were 20–700 μM, 2–402 μM and 0.4–252.4 μM, respectively, and the detection limits were 6.40 μM, 0.67 μM and 0.12 μM (S/N = 3). More importance, the proposed method offers promise for simple, rapid and cost-effective analysis of small biomolecules .
研究领域:
生物传感器
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2021
·
IF 4.464
Yu Tian,Jie Zhao,Dongxue Han,Shifan Zhao,Yuanwei Zhang,Guofeng Cui
In this paper, a novel two-step facile strategy was first proposed to fabricate nanoporous Ag (NP-Ag) and nanoporous Ag/AgCl (NP-Ag/AgCl) materials to simplify manufacturing procedure, shorten processing time, and optimize experiment condition. Co-electrodeposition of Ag Sn alloy on the interdigital gold electrode (IGE) and subsequent electrochemical dealloying in HCl solution by applying different potentials. The surface morphologies as well as the electrochemical characterizations of the fabricated NP-Ag and NP-Ag/AgCl IGEs were investigated. The fabricated NP-Ag IGEs were evaluated as biomolecules simultaneous electrochemical sensors for determination of acetaminophen (AC) and dopamine (DA). In addition, the performance of the fabricated NP-Ag/AgCl IGEs, as micro-size reference electrodes , were studied. The NP-Ag electrodes were found promising linear current response to AC (correlation coefficient, R 2 = 0.9906) and DA (correlation coefficient, R 2 = 0.9961), under the concentration ranges from 0.5 to 290 μM and 0.25 to 500 μM, respectively. Moreover, the fabricated NP-Ag/AgCl IGEs were possessed of enhanced stability and miniature size as pseudo-reference electrodes (p-REs) in contrast with commercial p-REs, which benefiting from abundant ion-exchange sites and quantities of nanochannels on the sponge-like surface. Results demonstrated the innovative surface modification of as-synthesized NP-Ag and NP-Ag/AgCl have positive implication for massive production of nanoporous Ag-based materials and the utilization of prospective point-of-care testing.
研究领域:
材料科学
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生物医学工程
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分析化学
✓ 已验证
DOI
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 58
·35007-35016
·
IF 3.119
Guo-Cheng Han,Huifang Li,Annaleizle Ferranco,Tao Zhan,Yunyun Cheng,Zhencheng Chen,Mingyue Xue,Xiao-Zhen Feng,Heinz-Bernhard Kraatz
Several simple sensors were fabricated through a one-step method. By depositing electro-active compounds, such as β-cyclodextrins (β-CD), heme, dopamine (DA), or Fc-ECG, onto a screen-printed electrode (SPE), the successful simultaneous detection of nitrite (NO2−) and thiosulfate (S2O32−) ions was observed. Under optimal operating conditions, the notable electrocatalytic abilities of a Heme/SPE sensor were detected for the oxidation of NO2− and S2O32−, with remarkable peak potential differences, after characterization via SEM, CV, and DPV. Linear relationships were obtained in the ranges of 5.0–200.0 μmol L−1 and 1.0–100.0 μmol L−1 for the current response versus concentration of NO2− and S2O32−, respectively. The limits of detection were determined to be 1.67 and 0.33 μmol L−1 while the sensitivities of detection were noted to be 0.43 and 1.43 μA μM−1 cm−2, respectively. During the detection of NO2− and S2O32−, no interfering common ions were observed. Furthermore, average recoveries from 96.0 to 104.3% and a total R.S.D. of less than 3.1% were found for the detection of NO2− and S2O32− in pickled juice and tap water using the simple sensor. These results showed that rapid and precise measurements for actual application in NO2− and S2O32− detection could be conducted in food samples, indicating a potential use in food safety.
研究领域:
分析化学
·
食品安全
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Chemosensors
·
2023
·Vol. 11
·期 9
·502
·
IF 4.2
Xinyu Gao,Siyu Chen,Xiaolei Wang,Honglei Liu,Xia Wang
Despite being present in minimal amounts, vitamin B2 (VB2), vitamin C (VC), and vitamin B6 (VB6) each play indispensable roles in human metabolisms. Given that VB2, VC, and VB6 cannot be synthesized by the human body, detections of these three vitamins both in fermentation liquid where vitamins are industrially manufactured and in human serum where vitamin concentrations could be clinically controlled are of significant importance. Here, a nanoporous gold (NPAu) modified screen-printed electrode (NPAu/SPE) was fabricated to detect VB2, VC, and VB6 based on NPAu’s electro-oxidation towards vitamins. Owing to the wide separation of peak potentials among VB2, VC, and VB6, the simultaneous detection of these three vitamins was achieved by the NPAu/SPE within a potential range from −0.8 V to 0.8 V. The achieved limits of detection (LOD) for VB2, VC, and VB6 were 0.46, 6.44, and 1.92 μM, with sensitivities of 68.58, 4.77, and 15.94 μA/μM, respectively. Subsequent reliability experiments suggested that the NPAu/SPE exhibited solid anti-interference capability and repeatability. Additionally, the real-sample detection of the NPAu/SPE towards VB2, VC, and VB6 was achieved both in human serum and in fermentation liquid with comparable accuracy (the recovery rates were from 89.8% to 111.7%) as high-performance liquid chromatography (HPLC). Moreover, the portable NPAu/SPE showed comparable performance in terms of the LOD and linear dynamic range when compared to glassy carbon electrodes (GCE) limited to laboratory detection. The proposed NPAu/SPE possesses various advantageous properties including portability, easy fabrication, high sensitivity, and cost-efficiency, making it a potential candidate for clinical and industrial multi-vitamins analysis.Keywords:screen-printed electrode;simultaneous detection;vitamin B2;vitamin C;vitamin B6
研究领域:
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
BioChip Journal
·
2020
·Vol. 14
·期 4
·429-437
·
IF 2.309
Xiong Xiaolu,Zhang Junlin,Wang Zhou,Liu Chenchen,Xiao Wende,Han Junfeng,Shi Qingfan
In order to improve the efficiency of disease diagnosis and environmental monitoring, it is desirable to detect the concentration of proteins and metal ions simultaneously, since the current popular diagnostic platform can only detect proteins or metal ions independently. In this work, we developed a colorimetric microfluidic paper-based analytical device (µPAD) for simultaneous determination of protein (bovine serum albumin, BSA) and metal ions [Fe(III) and Ni(II)]. The µPAD consisted of one central zone, ten reaction zones and ten detection zones in one device, in which reaction solutions were effectively optimized for different types of chromogenic reactions. Fe(III), Ni(II) and BSA can be easily identified by the colored products, and their concentrations are in good accordance with color depth based on the established standard curves. The detection limits are 0.1 mM for Fe(III), 0.5 mM for Ni(II) and 1µM for BSA, respectively. Best of all, we demonstrated the efficiency of the µPAD with accurate detection of Fe(III), Ni (II) and BSA from river water samples within 15 minutes. The µPAD detection is efficient, instrument-free, and easy-to-use, holding great potential for simultaneous detection of cross type analytes in numerous diagnostic fields.
研究领域:
分析化学
·
环境科学
·
医学诊断
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Gaosheng Zhao,Maolin Yang,Tingting Zhang,Bin Jia,Li Xu,Ping Cheng
Background The global release of organic and heavy metal components into natural water bodies is a major concern for the environment and human health. The assessment of water quality relies on analyzing organic and heavy metal components qualitatively and quantitatively. Real-time identification of organic and metal components in water systems requires different analytical techniques due to varying measurement requirements. Thus, on-line detecting both organic compounds and heavy metals in ambient water systems simultaneously using a single instrumentation setup presents a significant challenge. Results In this study, an analytical technique of nebulization-assisted injection plasma ionization mass spectrometry (NI-PIMS) was developed. This novel method enables the simultaneous detection of heavy metals and organic compounds in water system with high sensitivity, which has been demonstrated by the limit of quantification (LOQ) values below 1.0 μg/L for the three sterols (Enrofloxacin, ciprofloxacin , and clenbuterol) and three heavy metals (Pb, Ba, and Cd). Moreover, the method was successfully applied to rapidly analyze real water samples from urban and rural areas in China. The analytical results are available in less than 0.5 min, and only a few microliters of sample are required for each analysis. Significance and novelty As far as we know, this is the first report of on-line simultaneous analysis of organic compounds and heavy metals in a water system using a single mass spectrometry instrument. Compared to traditional methods, NI-PIMS demonstrates higher efficiency, sensitivity, no or lower sample preparation, and less sample consumption. The advancement and widespread use of this technology are expected to enhance the effectiveness of mass spectrometers , broaden the applications, and play an important role in complex sample analysis in fields such as atmospheric science , environmental science, and earth science.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2021
·Vol. 11
·期 11
·462
·
IF 5.519
Yangguang Zhu,Qichen Tian,Xiufen Li,Lidong Wu,Aimin Yu,Guosong Lai,Li Fu,Qiuping Wei,Dan Dai,Nan Jiang,He Li,Chen Ye,Cheng-Te Lin
Considering the vital physiological functions of dopamine (DA) and uric acid (UA) and their coexistence in the biological matrix, the development of biosensing techniques for their simultaneous and sensitive detection is highly desirable for diagnostic and analytical applications. Therefore, Ti3C2Tx/rGO heterostructure with a double-deck layer was fabricated through electrochemical reduction. The rGO was modified on a porous Ti3C2Txelectrode as the biosensor for the detection of DA and UA simultaneously. Debye length was regulated by the alteration of rGO mass on the surface of the Ti3C2Txelectrode. Debye length decreased with respect to the rGO electrode modified with further rGO mass, indicating that fewer DA molecules were capable of surpassing the equilibrium double layer and reaching the surface of rGO to achieve the voltammetric response of DA. Thus, the proposed Ti3C2Tx/rGO sensor presented an excellent performance in detecting DA and UA with a wide linear range of 0.1–100 μM and 1–1000 μM and a low detection limit of 9.5 nM and 0.3 μM, respectively. Additionally, the proposed Ti3C2Tx/rGO electrode displayed good repeatability, selectivity, and proved to be available for real sample analysis.
研究领域:
生物传感技术
·
材料科学
·
分析化学
✓ 已验证
DOI
高影响因子
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
·
2016
·Vol. 33
·期 7
·388-395
·
IF 4.367
Wenbin Wang,Liqiang Liu,Liguang Xu,Hua Kuang,Jianping Zhu,Chuanlai Xu
Staphylococcal enterotoxins (SEs) in food samples can cause severe gastroenteritis even at low doses. Detection of SEs with a single assay would not only improve reliability and portability, but would also save time. In this paper, for the first time, a multiplexed gold-nanoparticle-based immunochromatographic (ICT) strip that allows the simultaneous detection of the five main SEs has been reported: SEA, SEB, SEC, SED, and SEE. Based on the sandwich immunoassay of each SE, a multiplexed ICT strip with five test lines is developed. Sensitive and specific paired monoclonal antibodies facilitate the detection, allowing for high sensitivity and good specificity. The portable biosensor platform can simultaneously detect SEA, SEB, SEC, SED, and SEE at concentrations as low as 2.5, 2.5, 2.5, 1, and 5 ng mL −1 , respectively. The entire test can be performed within 15 min and requires no sophisticated instruments except a portable strip reader. To evaluate the multiplexed strip, naturally SEA and SEC contaminated milk samples and pure milk samples spiked with the five SEs are analyzed.
研究领域:
食品安全检测
·
免疫学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）