LUMINESCENCE
·
2013
·Vol. 29
·期 3
·243-247
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IF 1.273
F Meng,P Liu,F Huang,L Wang,X Wu,L Shen
In this study, a synchronous fluorescence detection method for ferulic acid (FA) is proposed based on a redox reaction between FA and Ce(IV) sulfate in dilute sulfuric acid medium at room temperature. It was found that FA could reduce Ce(IV) to Ce(III) in acidic medium, and sodium tripolyphosphate could further enhance the intrinsic fluorescence of the Ce(III) produced. The enhanced extent of synchronous fluorescence intensity was in proportion to the concentration of FA over the range 3.0 × 10 -8 to 1.0 × 10 -5 mol/L. The corresponding limit of determination ( S/N = 3 ) was 1.3 × 10 -8 mol/L. The proposed method was applied to the determination of sodium ferulate for injection sample with satisfactory results. Copyright © 2013 John Wiley & Sons, Ltd.
研究领域:
分析化学
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药物分析
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2017
·
IF 2.536
Ji Yang,Zheng-Zhong Lin,A-Zha Nur,Yan Lu,Ming-Hui Wu,Jun Zeng,Xiao-Mei Chen,Zhi-Yong Huang
A novel fluorescence-based sensor combining synchronous fluorescence spectroscopy (SFS) with molecularly imprinted polymers (MIPs) was fabricated with reverse microemulsion method. Tetracycline (TC), (3-aminopropyl) triethoxysilane (APTES), tetraethyl orthosilicate (TEOS) and carbon quantum dots (CDs) were used as template, functional monomer , cross-linker and signal sources respectively in the probe preparation. A synchronous fluorescence emission (λ em ) at 355 nm was observed for the prepared MIP-coated CDs (MIP@CDs) particles when the wavelength interval (Δλ) was set as 70 nm, and the synchronous fluorescence intensity could be rapidly and efficiently quenched by TC based on inner filter effect (IFE). The quenching efficiencies of synchronous fluorescence intensity was linearly fitted with tetracycline (TC) concentrations ranging from 0.1 to 50 μmol L − 1 with a detection limit (DL) of 9 nmol L − 1 (3 σ , n = 9). The MIP@CDs was used as a probe to detect TC in fish samples with the recoveries ranging from 98.4% to 103.1% and the relative standard deviation less than 6.0%. The results illustrated that the as-prepared MIP@CDs could be applied to the detection of trace TC in fish samples with rapidity, high sensitivity and accuracy.
研究领域:
分析化学
·
食品安全
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PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2025
·
IF 3.1
Zhang Hongmei,Yang Yunxia,Wang Yanqing
Sudan dyes are recognized as carcinogens, which are strictly determined whether there are them in food for food safety. Hence, in order to understand the mechanism at the molecular level, this work investigated the binding interactions of Sudan I-IV with calfthy mus DNA. The synchronous fluorescence and UV–vis spectral results suggested the complex formation between Sudan I-IV and ct-DNA. EB and Hoechst 33258 displacement experimental results confirmed the binding sites of Sudan I-IV in ct-DNA and the minor groove-binding mode of Sudan I-IV with ct-DNA. The molecular modeling computational method also proved binding mode, which is consistent with the spectral experiments. In addition, the aromatic rings of Sudan I-IV play important roles in binding with DNA. Compared to Sudan I and II, the increase of molecular conjugation system enhances the binding abilities of Sudan III and Sudan IV with DNA. Vdm + Hbond + desolv energies of them are the main contribution of their combined energies.
研究领域:
生物化学
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食品安全
·
分子生物学
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高影响因子
LUMINESCENCE
·
2016
·Vol. 31
·期 6
·1187-1193
·
IF 1.452
Lei Wang,Wei Li,Feiyu Zhang,Hui Li,Guimin Cai,Bing Li,Guangbei Qian,Yiping Du
Normal methods for benzo[a]pyrene (BaP) determination, including gas chromatography–mass spectrometry and liquid chromatography with fluorescence detection, involve expensive instruments and complex separation and purification processes. Based on membrane enrichment, coupled with solid-phase constant-wavelength synchronous fluorescence spectrometry, a simple, fast, sensitive method was proposed for the determination of BaP in water samples. A Nylon membrane was used as the solid-phase extraction material for enrichment. After enrichment, a constant-wavelength synchronous fluorescence spectrum was scanned directly on the membrane-concentrated BaP without elution. Spectral measurement and enrichment conditions were optimized. Under optimum conditions, when using 150 mL sample solutions, the relationship between fluorescence intensity and BaP concentrations in the 0.05–10.00 μg L –1 range could be fitted by binomial function with an R 2 value of 0.9973. Limit of detection (LOD) was calculated to be 0.0137 μg L –1 . The volume of the sample solution was increased to 1000 mL to test if the method could be applied to determine lower BaP concentrations. A linear relationship still existed in the range 2.0–20.0 ng L –1 BaP with an R 2 value of 0.9895, and a LOD of 2.4 ng L –1 . The method was also used to measure the BaP concentration in several natural water samples, and recoveries were in the 90–110% range with relative standard deviations (RSDs) in the 0.58–7.93% range. Copyright © 2016 John Wiley & Sons, Ltd.
研究领域:
分析化学
·
环境科学
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2019
·Vol. 43
·期 46
·18092-18097
·
IF 3.069
Muhammad Ajmal,Ali Abbas Falih Shindi,Yi-Hong Liu,Yan Zhao,Ping-Ping Wu,Jia-Wen Wei,Shyamal Kr Ghorai,Shuo Hui Cao,Yao-Qun Li
δ-Aminolevulinic acid (δ-ALA) plays a vital role in the diagnosis and clinical screening of diseases like lead poisoning, hepatic porphyria, and hereditary tyrosinemia type-1. However, because of the drawbacks of the existing methods, such as tedious pre-separations and analytical interference, it poses a serious challenge for an efficient, specific, and simple δ-ALA detection strategy. In this work, matrix-isopotential synchronous fluorescence spectrometry (MISFS) and the first derivative technique are combined for the direct determination of δ-ALA in urine samples. The proposed approach promises the detection of δ-ALA in urine samples with unknown background fluorescence, alleviating complicated and tedious pre-separation processes. Here, the fluorescent derivatization of δ-ALA was based on the Hantzsch reaction. MISF spectra were scanned along a matrix-isopotential trajectory, which combines the points of equal intensity in theoretical 3D contours. The contours were constructed from the excitation and emission spectra of δ-ALA and urine. The unknown background signal of the urine was suppressed by the first order derivative technique. Maximum sensitivity and appropriate selectivity were ensured by the study of the experimental variables in the δ-ALA bands, centered at its excitation and emission maxima (392.6 nm and 464.5 nm). The limits of detection and quantification, calculated according to IUPAC definitions, were 5.4 ppb and 18 ppb, respectively. The average recoveries were 97.2% ± 2.6%. For within-run precisions, the inter-day and intra-day CVs were 5.3% and 3.6%, respectively. The results achieved by the proposed method were consistent with those obtained by the conventional HPLC-FLD method.
研究领域:
分析化学
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医学诊断
·
生物化学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Shasha Cheng,Qianqian Kong,Xinyu Hu,Cuiling Zhang,Yuezhong Xian
Small extracellular vesicles (sEVs), often known as exosomes, are expected to be a promising biomarker for the early diagnosis of cancer because they carry enriched proteins that originated from parent cells. Profiling surface proteins of sEVs offers non-invasive access for the early diagnosis of cancer. However, it remains challenging to simultaneously detect surface proteins of sEVs with desired sensitivity. Herein, a dual color DNA nanodevice based on toehold-mediated DNA strand displacement signal amplification and the synchronous fluorescence technique has been developed for simultaneous analysis of surface proteins of sEVs with high sensitivity. As for the DNA nanodevice-based system, the nanoconjugates of aptamer-magnetic beads can recognize surface proteins of sEVs and lead to the release of single-stranded DNA. Then, the released DNA can trigger toehold-mediated DNA strand displacement for signal amplification. In this system, a CD63 aptamer and MUC1 aptamer were used as recognition elements for the detection of surface proteins of sEVs isolated from cancer cells. Under the optimal conditions, the corresponding proteins of sEVs were simultaneously determined with ultrasensitivity by the synchronous fluorescence method. Also, the detection limits of sEVs by two surface proteins were 67 particles/μL by CD63 and 37 particles/μL by MUC1. Of note, the as-constructed method can be applied to recognize sEVs from different tumor cell lines (SGC7901, HepG2, and MCF-7 cells). Furthermore, the system has been successfully applied to precisely identify cancer patients from healthy people by serum analysis. The strategy demonstrates great potential applications in the early diagnosis of cancer.
研究领域:
生物化学
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医学诊断
·
分子生物学
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PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Jian Wei,Jianjun Sun,Yucui Hou,Zhuosen He,He Li,Shuhang Ren,Weize Wu
The structural analysis of lignin is highly significant in practical terms. We investigated the presence of fused aromatic multirings (FARs) distinct from conventional phenylpropane units in seven lignin types using synchronous fluorescence spectroscopy (SFS) and quantified their relative proportions. Our findings reveal that FARs are present in all lignin samples, primarily composed of 2–6 rings. The relative distribution of FARs in lignin is ranked as follows: 2 rings > 1 ring > 4 rings > 3 rings > 6 rings > 5 rings. The relative contents of these FARs (1–6 rings) exhibit slight variations across all lignin samples. Notably, FARs with 2 rings are the highest at 35 %−53 %, while those with 5 and 6 rings are the lowest, collectively accounting for less than 1.37 %. Moreover, the relative contents of FARs with 1 and 2–6 rings range approximately 24 %−32 % and 68 %−76 %, respectively. Furthermore, the catalytic hydro-depolymerization of lignin was performed and the experimental results demonstrate that the hydro-liquefied oil from lignin contains aromatic compounds with 1–7 rings, and the relative contents of aromatic compounds with 1 ring and 2–7 rings in the oil are 29 % and 71 %, respectively, which is consistent with that of lignin SFS. These findings are also consistent with the existing literature and experimental results. This study offers an explanation for the low yields observed in current depolymerization processes targeting small molecules within lignin and establishes a theoretical foundation for achieving high yields in the preparation of multiring aromatic hydrocarbons.
研究领域:
化学工程
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分析化学
·
生物技术
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高影响因子
WATER RESEARCH
·
2024
·
IF 11.4
Xiaodan Zheng,Menglin Wang,Shiqi Zhang,Zhima Yangcuo,Lifang He,Lingfang Xie,Yurou Ye,Guifen Xu,Zhonghui Chen,Qihong Cai
Antibiotics are a new type of environmental pollutants. Due to its wide application in many fields, antibiotic residues are ubiquitous in the wastewater environments. Given their potential threat on water ecosystem functioning and public health, the detection of antibiotic residues in wastewater environments has become very necessary. Based on the complexation of Al 3+ with flumequine (FLU), ciprofloxacin (CIP) and doxycycline hydrochloride (DOX), their molecular conjugated area were increased and fluorescence intensity were enhanced, combined with synchronous fluorescence spectrometry (SFS) had good selectivity and high sensitivity, a novel method of Al 3+ sensitized synchronous fluorescence spectrometry for the determination of FLU , CIP and DOX residues in wastewater was established. When the wavelength difference (Δλ) was selected 115.0 nm, synchronous fluorescence spectra of the three antibiotics could be well separated and the interference of wastewater matrix were eliminated primely. The new SFS made good use of spectral separation instead of conventional chemical separation, and the actual wastewater sample could be directly determined after simple filtration. The experiment results showed that the concentrations of FLU, CIP and DOX in the range of 0.5000–800.0 ng·mL -1 , 0.5000–640.0 ng·mL -1 and 10.00–3500 ng·mL -1 had a good linear relationship with fluorescence intensity. The detection limits of three antibiotics were 0.02054 ng·mL -1 , 0.03956 ng·mL -1 and 0.8524 ng·mL -1 , respectively. Recovery rates of three antibiotics in wastewater samples were 90.72%-98.23%, 88.68%-95.08% and 85.94%-96.70%. The new SFS established in this experiment had the advantages of simple, rapid, sensitive, accurate and good selectivity. Simultaneous and rapid detection of FLU, CIP and DOX residues in wastewater was successfully realized. It had good application prospects in real-time water quality monitoring.
研究领域:
环境科学
·
分析化学
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PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 3
·1-12
·
IF 5.3
Li Lingling,Zhang Xinru,Chang Xianglei,Meng Xianhua,Wang Weifeng,Zhang Ying,Yang Aimei,Yang Junli
A highly selective and sensitive silicon nanoparticles (Si NPs) biosensor was developed for dopamine (DA) detection utilizing synchronous fluorescence spectroscopy (SFS). Si NPs were synthesized via a one-step hydrothermal method, utilizing 3-aminopropyltriethoxysilane (APTES) as the silane precursor and polyethyleneimine (PEI) as the reducing agent. The Si NPs exhibited a spherical morphology, with an average diameter of 3.7 nm. Additionally, the Si NPs demonstrated remarkable fluorescent properties. When integrated into the DA detection process utilizing SFS, the synchronous fluorescence spectrum of the DA system exclusively exhibits a peak at 530 nm, whereas systems involving epinephrine and norepinephrine both display peaks at 484 nm and 530 nm. Consequently, this detection system exhibits a high capacity for effectively differentiating between DA and other catecholamines (epinephrine and norepinephrine), ensuring a high degree of specificity in the detection process. Additionally, this combined approach also presents a strong linear relationship between the enhancement of synchronous fluorescence intensity (F 530 /F 484 ) and DA concentrations within the concentration ranges 1–10 μ M and 10–50 μ M, respectively. The limit of detection (LOD) was 0.22 μ M (3 σ / k ). Finally, the biosensor was successfully employed for the detection of DA in the spiked serum samples. This work introduced a novel approach for DA detection and provided a valuable technical platform for rapid monitoring of DA levels in biological samples. Graphical abstract
研究领域:
分析化学
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生物医学工程
·
纳米技术
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高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Qihong Cai,Menglin Wang,Zhima Yangcuo,Xiaodan Zheng,Xinyi Zheng,Yaning Qin,Zhexiang Zou,Shiqi Zhang,Zhonghui Chen,Zhangxu Chen
In this study, a new first derivative synchronous fluorescence spectrometry (SFS) for the accurate and sensitive determination of pefloxacin (PFX) and fleroxacin (FLX) in wastewater was established. PFX and FLX were complexed with Al 3+ to significantly enhance their fluorescence intensity. Because the conventional fluorescence spectra of PFX-Al 3+ and FLX-Al 3+ overlap significantly, the wavelength difference (Δλ = 155 nm) was chosen for SFS scanning to narrow spectral band. And the narrowed synchronous fluorescence spectra was subjected to first derivative processing. Finally, the zero intersections of the first derivative synchronous fluorescence spectra at 281.6 nm and 273.6 nm were chosen for the determination of PFX and FLX, cleverly used of spectral parameters to eliminate the serious interference between PFX-Al 3+ and FLX-Al 3+ and the interference of wastewater background. The limits of detection of PFX and FLX were 0.0086 ng·mL −1 and 0.0068 ng·mL −1 with linear ranges from 0.1–40 ng·mL −1 and 0.2–80 ng·mL −1 , respectively. Recoveries of PFX and FLX in wastewater were 88.60 %–98.60 % and 90.00 %–95.60 %. The first derivative SFS established in this study used spectral separation instead of chromatographic separation, so that sample pretreatment was simple and the detection speed was fast. Compared the conventional method of HPLC, the test results of above two methods were close. And the new method had been successfully used for the determination of PFX and FLX residues in wastewater with good results. Therefore, the new first derivative SFS was expected to become a routine detection technology for PFX and FLX residues in wastewater and widely used.
研究领域:
分析化学
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环境科学
·
药物分析
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高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 4
·2640-2648
·
IF 3.201
Ji Yang,Ming-Hui Wu,Zheng-Zhong Lin,Zhi-Yong Huang
A novel fluorescence nanoprobe using CdTe quantum dots as signal sources coated with a molecularly imprinted polymer was synthesized by a reverse microemulsion method. The nanoprobe was designed for the detection of leucomalachite green (LMG) in fish samples via synchronous fluorescence quenching based on the inner filter effect. LMG, (3-aminopropyl) triethoxysilane and tetraethyl orthosilicate were used as the template, functional monomer and cross-linker respectively in the probe. A synchronous fluorescence emission of the probe at 260 nm was observed when the wavelength interval was set as 340 nm, and the synchronous fluorescence could be linearly quenched with LMG at concentrations ranging from 0.1 to 20 μmol L−1. The as-prepared nanoprobes were nearly spherical with a diameter of about 50 nm. The detection limit was 23 nmol L−1 (3σ, n = 9) and the recoveries ranged from 96.2% to 104.9% for the fish samples. The results illustrated that the nanoprobe could be used for trace LMG measurement in fish samples.
研究领域:
分析化学
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环境科学
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食品科学
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高影响因子
CHEMISTRY & BIODIVERSITY
·
2024
·Vol. 21
·期 7
·e202400634
·
IF 2.3
Yongshan Ma,Qingxiang Guan,Jingcheng Cui,Tianyi Jiang,Yanyan Zhu,Fengxia Zhang,Guangxiang Ma
Molybdenum disulfide nanoflowers (MoS 2 NFs) were prepared by hydrothermal method. The prepared MoS 2 NFs was characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD), specific surface areas, Raman and X-ray photoelectron spectroscopy (XPS). The characterization results show that the flower-like spherical MoS 2 is composed of many ultra-thin nanosheets with an average diameter of about 300–400 nm. MoS 2 NFs also exhibits excellent UV–vis absorption and high fluorescence intensity. In order to explore the biological behavior of MoS 2 NFs, the interaction between MoS 2 NFs and bovine serum albumin (BSA) was studied by UV–Vis absorption, fluorescence, synchronous fluorescence spectra, and cyclic voltammetry. The results of absorption and fluorescence show that MoS 2 NFs and BSA interact strongly through the formation of complexes in the ground state, and the static quenching is the main mechanism. The Stern–Volmer constant and the quenching constant was calculated about 3.79×10 7 L mol −1 and 3.79×10 15 L mol −1 s −1 , respectively. The synchronous fluorescence implied that MoS 2 in the complex may mainly bind to tryptophan residues of BSA. The cyclic voltammograms indicated that the addition of BSA makes electron reduction of MoS 2 NFs more difficult than the corresponding free state. The results show that hydrophobic forces play a major role in the binding interaction between BSA and MoS 2 NFs. Graphical
研究领域:
材料科学
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生物化学
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分析化学
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PubMed
高影响因子
危险品化学品经营许可证（带存储）