Cell Host & Microbe
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2026
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IF 23.2
Fengqing Lin,Lianglan Li,Yuanpeng He,Shujie Xu,Qiufen Mo,Wangsen Cao,Aikun Fu,Weiqin Li
The enteric microbiome and nutrient sensing within the small intestine play critical roles in maintaining host metabolic homeostasis. Although various bacteria and some fungi have established functions in nutrient metabolism, the role of the enteric virome remains poorly understood. Here, we demonstrate that the enteric virome significantly influences carbohydrate digestion and absorption independently of the bacteriome. Furthermore, the virome elicits distinct responses across different intestinal cell types. Specifically, it activates programs for carbohydrate digestion and absorption in intestinal epithelial cells while simultaneously stimulating antigen-presenting cells―Th17 cells―to produce interleukin-22, a cytokine that curbs excessive carbohydrate uptake. The virome’s effect on carbohydrate digestion and absorption—whether suppressive or stimulatory—depends on the presence or absence of immune surveillance. This intricate interplay between metabolic and immune pathways establishes the enteric virome as a pivotal regulator of metabolism and reveals the virome’s intrinsic capacity to autonomously modulate vertebrate intestinal physiology.
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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2023
·Vol. 24
·期 13
·10744
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IF 5.6
Mingyue Hao,Minghui Wang,Danyu Zhao,Yong Shi,Ye Yuan,Junmei Li,Yunyi Zhai,Xiaofang Liu,Dong Zhou,Huatao Chen,Pengfei Lin,Keqiong Tang,Wei Liu,Yaping Jin,Aihua Wang
Brucella suis, the causative agent of brucellosis, poses a significant public health and animal husbandry threat. However, the role of the alanine racemase (alr) gene, which encodes alanine racemase inBrucella, remains unclear. Here, we analyzed analrdeletion mutant and a complemented strain ofBrucella suisS2. The knockout strain displayed an unaltered, smooth phenotype in acriflavine agglutination tests but lacked the core polysaccharide portion of lipopolysaccharide (LPS). Genes involved in the LPS synthesis were significantly upregulated in the deletion mutant. Thealrdeletion strain exhibited reduced intracellular viability in the macrophages, increased macrophage-mediated killing, and upregulation of the apoptosis markers. Bcl2, an anti-apoptotic protein, was downregulated, while the pro-apoptotic proteins, Bax, Caspase-9, and Caspase-3, were upregulated in the macrophages infected with the deletion strain. The infected macrophages showed increased mitochondrial membrane permeability, Cytochrome C release, and reactive oxygen species, activating the mitochondrial apoptosis pathway. These findings revealed that alanine racemase was dispensable inB. suisS2 but influenced the strain’s rough features and triggered the mitochondrial apoptosis pathway during macrophage invasion. The deletion of thealrgene reduced the intracellular survival and virulence. This study enhances our understanding of the molecular mechanism underlyingBrucella’s survival and virulence and, specifically, howalrgene affects host immune evasion by regulating bacterial LPS biosynthesis.Keywords:Brucella suisS2;alanine racemase;alr;lipopolysaccharide;apoptosis;mitochondrial membrane potential;intracellular survival
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2023
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IF 4.4
Huajie Chen,Dai Li,Yutao Zheng,Kui wang,He zhang,Zhipeng Feng,Bolin Huang,Huiyun Wen,Jiyong Wu,Weiming Xue,Saipeng Huang
Hypochlorite (ClO - ) is an important redox regulator in reactive oxygen species, which play a considerable role in oxidative stress and related diseases. Hence, accurate and sensitive monitoring of ClO - concentration was urgently needed in the fields of life sciences, food and environment. Bright green fluorescent carbon quantum dots (G-CQDs) were synthesized utilizing one-step hydrothermal method with citric acid and acriflavine precursors. Through TEM, FTIR, XPS and zeta potential characterization procedures, G-CQDs illustrated uniformly dispersed and significant number of –NH 2 and –OH on the surface. Meanwhile, the fluorescence and colorimetric analysis exhibited wide linear range and low detection limit response to ClO - . The fluorescence changes of G-CQDs were identified via smartphone to realize mobile sensing of ClO - . Subsequently, G-CQDs was applied for visualization and quantitative detection of ClO - in drinking water samples with satisfactory recovery rate. More importantly, G-CQDs demonstrated good water solubility, optical stability and excellent biocompatibility, which offered a promising analysis approach in cell imaging and exogenous ClO - detection in living cells. G-CQDs illustrated bright prospect and great potential in practical application of ClO - associated disease prevention and early clinical diagnosis.
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Molecular Catalysis
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2020
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IF 3.687
Xuyang Chen,Yu Wei,Wenxuan Sun,Xiangfen Meng,Shengjie Hao,Yan Gao
Achieving selective photocatalytic conversion of CO 2 in an aqueous solution is challenging owing to the generation of hydrogen as a typical by-product. Herein, a visible light-driven CO 2 conversion system was developed using Co(bpy) 2 Cl 2 as the catalyst and Acriflavine (Acr) as the photosensitizer (PS). At the optimal ratio, under visible light irradiation, the system achieved high selective CO 2 conversion to CO (∼100 %) in an aqueous acetonitrile solution, with a turnover number (TON) of 100 and a maximal turnover frequency (TOF) of 90 h −1 . Deeply studies reveal that the -NH 2 of Acr plays a vital effect on the selectivity of this photocatalytic CO 2 conversion system. The unique combination of Co(bpy) 2 Cl 2 and Acr provides an ideal platform for designing highly selective photocatalytic systems for CO 2 reduction.
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JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
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2019
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IF 2.606
Zhi-Cheng Yao,Jun-Chuan Wang,Baolin Wang,Zeeshan Ahmad,Jing-Song Li,Ming-Wei Chang
Recent explorations have shown potential drug delivery applications of random and aligned fibrous dosage forms (e.g. patches, dressings, films and inserts). In this study, novel controlled delivery dosage forms, comprising Janus fibers, are designed and fabricated using electrohydrodynamic (EHD) engineering. Model probe and active compound release is shown from dissimilar polymer systems (non-soluble and soluble in nature) which constitute towards Janus systems. Micron scaled aligned Janus fibers comprising polycaprolactone (PCL) and polyvinyl pyrrolidone (PVP) were engineered to host rhodamine B and acriflavine , respectively. The impact of various operating parameters (e.g. flow rate, collector speed, working distance and applied voltage) on resulting mean fiber diameter and shape were optimized. Tensile strain decreased from 116.5 ± 10.2% to 81.8 ± 9.5% upon the inclusion of the model compounds, while both PCL/PVP and PCL/PVP/dyes samples exhibited similar tensile strength (∼0.65 MPa). FTIR analyses demonstrates PCL/PVP blend behavior and successful incorporation of rhodamine B and acriflavine. Acriflavine release from PCL fibers spanned over a 4-day period, and release of rhodamine B from PVP fibers was just over 6 h. The results indicate the EHD direct-jetting technique enables the precise construction of drug loaded and aligned fibers in a single-step with minimal excipient usage for potential customized drug delivery.
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MICROCHEMICAL JOURNAL
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2019
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IF 3.206
Juan Yue,Li Li,Peng Miao,Zheng Wang,Zhimin Chang,Dan Shao,Haohua Shao,Qian Mei,Shi-zhong Luo,Wen-Fei Dong
Adenine detection is important in biomedicine. Here, we report acriflavine-based carbon dots prepared via a one-step synthesis that can detect adenine in aqueous solution via fluorescence. Titration experiments show that the fluorescence intensity of carbon dots increases by up to 35% as the concentration of adenine increases. High-resolution transmission electron microscopy images show that carbon dots have a recognizable lattice structure. Based on this, we built a theoretical model using the DFT method to explain the mechanism by which adenine enhances fluorescence on the carbon dots.
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TALANTA
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2018
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IF 4.244
Xiu-Fang Yan,Zeng-Ping Chen,Ying Huang,Chao Kang,Ru-Qin Yu
Probe encapsulated by biologically localized embedding (PEBBLE) has emerged as a new type of sensing technique for complex systems. Generalized ratiometric PEBBLE nanosensors prepared by encapsulating an intensity-based probe and an inert reference dye inside the pores of stable matrix possess advantages of easy synthesis, immunity to interference, lower toxicity, and robustness to variations in probe loading. However, the selection of appropriate reference dyes used in generalized ratiometric PEBBLE nanosensors is a rather difficult task since they should satisfy some stringent requirements. In this contribution, the feasibility of using carbon dots (C-dots) as generic inert references in synthesizing PEBBLE nanosensors was first investigated in detail. And a dual-wavelength monitoring strategy and the quantitative fluorescence model for generalized ratiometric probes (QFM GRP ) were adopted to solve the problems brought by the use of carbon dots as inert references. C-dots doped PEBBLE nanosensors (C-PEBBLE nanosensors) for the quantification of NO 2 − and free Ca 2+ were synthesized by encapsulating C-dots and intensity based fluorescence probes (i.e., acriflavine for NO 2 − , and Rhod-2 for Ca 2+ , respectively) inside the pores of stable matrix. Experimental results showed that the combination of C-PEBBLEs, the QFM GRP model and the dual-wavelength monitoring strategy achieved accurate quantification of NO 2 − and the free Ca 2+ in real-world samples. Their quantitative results were in good consistence with those determined by HPLC and atomic absorption spectrophotometer, respectively. The strategies proposed in this contribution have generic applicability in the synthesis of PEBBLE nanosensors and their quantitative applications.
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REACTIVE & FUNCTIONAL POLYMERS
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2017
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IF 3.151
Zehu Wang,Teng Qiu,Longhai Guo,Jun Ye,Lifan He,Xiaoyu Li
The adsorptive materials which have the function of molecular recognition are becoming important as the fast emerging environmental requirements for the analysis and repair of target contaminants present at low concentrations in aqueous matrices. Here, we demonstrate a Pickering emulsion polymerization strategy to build bisphenol A (BPA) imprinted poly(methacrylic acid) microspheres without the traditional organic surfactant . In the synthesis, the only stabilizer is silica particles derived from Stöber process. The molecularly imprinted polymer microspheres were prepared by radical polymerization in the Pickering oil/water emulsion. Both the solid stabilizers and the molecular templates were conveniently removed from the microspheres. The obtained microspheres are of regularly spherical structures and hydrophilic surfaces . The formation of molecularly imprinted sites on the microspheres was validated by the excellent recognition capability toward BPA in the rebinding and competitive binding experiments.
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Journal of Inorganic and Organometallic Polymers and Materials
·
2024
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IF 3.9
Li Mengke,Cao Panpan,Dong Wenwen,Zhao Jun,Li Dongsheng
Organic dyes pollution has caused significant threat to flora, fauna and humans because the toxic of dyes. In this work, with the help of acetic acid as a defect initiator, the hierarchical porous MIL-101(HP-MIL-101) has been synthesized and used for removing anionic dyes Eosin y (EY), Methyl orange (MO), Congo red (CR) and Acid fuchsin (AF) from water. Due to its positive surface charge, larger specific surface area, well-matched pore structure, and the charge, size, and diameter of dyes, HP-MIL-101 showed rapid adsorption and an excellent maximum adsorption capacity of 425 and 336 mg⋅g − 1 for CR and AF, respectively. A series of batch experiments were carried out to investigate the impact of various key factors, including adsorption duration, initial dye concentration, and temperature. In addition, the kinetics, isotherm, and thermodynamics about the adsorption properties were also discussed. In addition, HP-MIL-101 showed stable and high reusability for at least three cycles, with an efficiency of over 95% without significant structural changes. This study highlights the potential of hierarchical porous MOF for mitigating dye pollution and advancing sustainable water treatment methodologies.
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FOOD CHEMISTRY
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2024
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IF 8.5
Yuandong Lin,Ji Ma,Da-Wen Sun,Jun-Hu Cheng,Chenyue Zhou
A fluorescent sensor array (FSA) combined with deep learning (DL) techniques was developed for meat freshness real-time monitoring from development to deployment. The array was made up of copper metal nanoclusters (CuNCs) and fluorescent dyes, having a good ability in the quantitative and qualitative detection of ammonia, dimethylamine, and trimethylamine gases with a low limit of detection (as low as 131.56 ppb) in range of 5 ∼ 1000 ppm and visually monitoring the freshness of various meats stored at 4 °C. Moreover, SqueezeNet was applied to automatically identify the fresh level of meat based on FSA images with high accuracy (98.17 %) and further deployed in various production environments such as personal computers, mobile devices, and websites by using open neural network exchange (ONNX) technique. The entire meat freshness recognition process only takes 5 ∼ 7 s. Furthermore, gradient-weighted class activation mapping (Grad-CAM) and uniform manifold approximation and projection (UMAP) explanatory algorithms were used to improve the interpretability and transparency of SqueezeNet. Thus, this study shows a new idea for FSA assisted with DL in meat freshness intelligent monitoring from development to deployment.
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危险品化学品经营许可证（带存储）