JOURNAL OF RAMAN SPECTROSCOPY
·
2020
·Vol. 51
·期 4
·642-648
·
IF 2
Wei Li,Xiaozhou Li,Tianyue Yang,Xingjia Guo,Youtao Song
An immunochromatographic assay (ICA) based on surface-enhanced Raman scattering (SERS) method was developed for the detection of morphine in this study. Indirect competitive immunoadsorption was adopted in the ICA due to the small molecular weight of morphine. Antibody modified gold nanoparticles in the ICA were labeled with Raman reporters and were used as the SERS enhancement substrate. By measuring the feature peaks of Raman reporter MBA at 1,078 cm −1 , a quantitative estimation of morphine can be obtained. The limit of detection of this ICA-SERS method was 2.4 × 10 −4 ng/ml for morphine, which was four orders of magnitude lower than the visual method. This study demonstrated that the proposed ICA-SERS method is a good candidate for the rapid and accurate detection of illicit drugs in saliva. All the analyses in this paper were carried out in a Microsoft (MS) Excel platform using embedded R functions through Excel BERT add-in.
研究领域:
分析化学
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2023
·
IF 2.9
Qinhong Yin,Xiaolan Yang,Lihua Yang,Dezhi Yang,Yaling Yang,Yanqin Zhu
As newly developed synthetic enzymes with exceptional catalytic capabilities and outstanding stability, nanozymes have drawn considerable interest in the realm of sensing. Using a simple hydrothermal process, iodine and copper-doped carbon dots (Cu,I-CDs) with simulated enzymes were fabricated in the current investigation. Cu,I-CDs demonstrate peroxidase-mimicking function together with high catalytic effectiveness due to aforementioned features. This led to generation of a colorimetric sensor for quick and accurate quantitative assessment of morphine (MOR). The outcomes showed the method's usefulness for the colorimetric detection of MOR. The linear range for MOR detection is 0.25–25 μg/mL having a reduced detection limit of 64 ng/mL. This sensor's successful use in the analysis of MOR in biological material is more noteworthy.
研究领域:
生物传感
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纳米酶研究
·
医学检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR
·
2012
·
IF 2.532
Haichen Niu,Yingwei Zheng,Tanzeel Huma,Joshua D. Rizak,Ling Li,Guimei Wang,He Ren,Liqi Xu,Jianzhen Yang,Yuanye Ma,Hao Lei
Previous studies have shown that olfactory impairment by disrupting the olfactory epithelium prior to morphine administration attenuated the development addiction-related behaviors . However, it is unclear whether olfactory impairment will affect the expression of already established addiction-related behaviors. To address this issue, mice were conditioned with morphine to induce behavioral sensitization and condition placed preference (CPP). After an abstinence period, the animals were subjected to either an intranasal ZnSO 4 effusion (ZnE) or sham treatment with saline. Behavioral sensitization and CPP reinstatement were evaluated 24 h later, as well as the expression of c-Fos protein, a marker of activated neural sites, in brain regions of interest. It was found that ZnE treatment attenuated morphine-induced behavioral sensitization and reinstatement of CPP. Compared to the saline-treated ones, the ZnE-treated animals showed reduced c-Fos expression in the nucleus accumbens (NAc) associated with behavioral sensitization, and in the NAc, cingulate cortex , dentate gyrus , amygdala , lateral hypothalamus and ventral tegmental area associated with CPP reinstatement. Together, these results demonstrated that acute olfactory impairment could attenuate already established addiction-related behaviors and expression of c-Fos in drug addiction related brain regions, perhaps by affecting the coordination between reward and motivational systems in the brain.
研究领域:
神经科学
·
药理学
✓ 已验证
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Lingyi Zhao,Yi Wei,Hao Fu,Ruiqin Yang,Qian Zhao,Hongwen Zhang,Weiping Cai
The accurate, sensitive and portable detection of morphine is important to handle judicial cases, but remains to be a great challenge. In this work, a flexible route is presented for the accurate identification and efficient detection of trace morphine in solutions based on surface-enhanced Raman spectroscopy (SERS) and a solid substrate/chip. A gold-coated jagged silicon nanoarray (Au-JSiNA) is designed and prepared via Si-based polystyrene colloidal template-reactive ion etching and sputtering deposition of Au. Such Au-JSiNA has three-dimensional nanostructure with good structural uniformity, high SERS activity and hydrophobic surface . Adopting this Au-JSiNA as SERS chip, trace morphine in solutions could be detected and identified in both dropping and soaking ways, and the limit of detection is below 10 -4 mg/mL. Importantly, such chip is especially suitable for the detection of trace morphine in aqueous solutions and even domestic sewage. The good SERS performance is attributed to the high-density nanotips and nanogaps on this chip as well as its hydrophobic surface. Additionally, the appropriate surface modification of this Au-JSiNA chip with 3-mercapto-1-propanol or 3-mercaptopropionic acid/1-(3-dimethylaminopropyl)-3-ethylcarbodiimide can further increase its SERS performances to morphine. This work provides a facile route and practical solid chip for SERS detection of trace morphine in solutions, which is significant to develop the portable and reliable instruments for on-site analysis of drugs in solutions.
研究领域:
分析化学
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法医学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2022
·
IF 9.231
Xuan Zhang,Jin Wang,Jianhao Liang,Zhiwei Liu,Xing Shen,Yingju Liu,Xiangmei Li,Zhenlin Xu,Yi Lei,Hongtao Lei
Opium poppy abused in food has aroused public concerns due to its serious side effects. Effective monitoring is essential to fight the abuse crisis. Herein, we synthesized an easily prepared, affordable, accessible highly aggregated gold nanoparticles (AGNPs) performing in lateral flow immunoassay (LFIA) for detection opium poppy in herbal teas. Simultaneously, a LFIA based on time-resolved fluorescent microspheres (TRFMs) was developed as contrastive method. In this study, morphine (MOR), codeine (COD) and thebaine (THE) were as the specific recognition markers of opium poppy. Results demonstrated the quantitative limits of detection were 0.0049/0.0053/0.084, 0.034/0.037/0.37 ng mL −1 for AGNPs/TRFMs-LFIA, respectively. The recoveries were 95%-107.5%/91%-106.7% with coefficient of variation was 1.6%-6.6%/1.8%-7.2%, indicating excellent accuracy and precision. Parallel experiments among AGNPs/TRFMs-LFIA and LC-MS/MS analysis showed good correlation. Overall, AGNPs-LFIA executed quantitative analysis within 15 min on the basis of simple treatment while providing a rapid and sensitive analysis strategy for illegal drugs abused.
研究领域:
分析化学
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毒理学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
RSC Advances
·
2021
·Vol. 11
·期 27
·16805-16813
·
IF 3.361
Qinhong Yin,Mengtao Wang,Dong Fang,Yanqin Zhu,Lihua Yang
In the present study, new N,Cl co-doped carbon dots (N,Cl-CDs) based on deep eutectic solvent (DES) were fabricated by a facile hydrothermal process. This fluorescent probe exhibited a good quantum yield of 14% and was applied for the sensitive and selective quantification of morphine in foods. In addition, the influence of solution pH, interaction time, system temperature, interfering substances and analogues on the determination was also investigated. Under the optimized conditions, the luminescence intensity of carbon dots increased linearly with the addition of morphine in the concentration range of (0.15–280.25) μg mL−1 (R2 > 0.9969) and the limit of detection (LOD) of 46.5 ng mL−1. Based on these results, it is suggested that N,Cl-CDs is a promising fluorescent probe for sensitive and selective quantification of morphine in foods.
研究领域:
分析化学
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食品科学
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材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROANALYSIS
·
2021
·Vol. 33
·期 6
·1471-1483
·
IF 3.223
Shufang Ren,Runyan Feng,Shounian Cheng,Qingtao Wang,Zhixiang Zheng
Ti 3 C 2 T X MXene was synthesized by exfoliating pristine Ti 3 AlC 2 phase with hydrofluoric acid. The simple methods of mechanical mixing and drop-casting of Ti 3 C 2 T X and MWCNTs were carried out to prepare sensing electrode of Ti 3 C 2 T X /MWCNTs/GCE. The composite and topography, especially the surface functional groups of Ti 3 C 2 T X /MWCNTs were analyzed by XRD, SEM, FTIR, XPS, and Raman spectrum. The results turned out that Ti 3 C 2 T X was characteristic by accordion-like 2D nanostructure with the surfaces terminated with −OH, −F, and =O. When combining with acid pretreated, the interaction between the functional groups of Ti 3 C 2 T X and MWCNTs facilitated the convenience and reproducibility of the robust modified electrodes and could make Ti 3 C 2 T X /MWCNTs/GCE possess good synergistic catalytic acceleration by increasing the electron transfer efficiency as well as adsorption and aggregation of MOP analyte onto the electrode surface. Versatile electrochemical measurements of CV, DPV and EIS were used to investigate the electrochemical performance of Ti 3 C 2 T X /MWCNTs/GCE sensing platform. The linear detection range is 0.01–100 μM with the limit of detection of 0.0092 μM (S/N=3). The sensor has good stability, repeatability, reproducibility and anti-interference. In the detection of serum and urine samples, it has a good recovery rate.
研究领域:
材料科学
·
分析化学
·
医学检测
✓ 已验证
产品关联
DOI
高影响因子
ACS Sensors
·
2020
·
IF 7.333
Wei Chen,Lijun Yang,Chao Yan,Bangben Yao,Jianfeng Lu,Jianguo Xu,Guodong Liu
Overuse and abuse of morphine (MOP), one of the main components of pericarpium papaveris, have attracted increasing attention in the medical field owing to its pharmacological and toxicological activity. Herein, we proposed a new electrochemical nano-biosensor for MOP detection based on surface-confined building of Au@Pt-centered and multi-G-quadruplex/hemin wire-surrounded electroactive super-nanostructures. The center Au@Pt was flower-shaped and irregularly protruded, allowing substantial loading of multiple G-quadruplex wire/hemin complexes on its surface to accomplish the assembly of electroactive super-nanostructures. Interestingly, as the super-nanostructures were closely confined on the electrode surface, a significantly amplified electrochemical signal was thus obtained in the absence of MOP. In contrast, the introduction of target MOP can induce an intense competitive effect and strongly destroy the assembly process, resulting in the reduction of the electrochemical response that is correlated with the logarithmic concentration of MOP. Under optimal conditions, the electrochemical nano-biosensor is capable of highly sensitive detection of MOP in a dynamic concentration range from 1 ppt to 500 ppb. The limit of detection is achieved as low as 0.69 ppt, and the practical application was confirmed by examining MOP from chafing dish condiments. We expect the electrochemical platform utilizing this unique nanoarchitecture to provide rational guidelines to design high-performance analytical tools.
研究领域:
分析化学
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医学检测
·
纳米技术
✓ 已验证
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2016
·
IF 3.926
Shuangling Xi,Kai Zhang,Deli Xiao,Hua He
In this work, a novel magnetic ultra-thin dummy molecularly imprinted polymer (MMIP) for morphine (MO) was prepared. In order to obtain highly selective recognition cavities, the MMIP has been designed using semi-flexible docking to screen the optimal monomer and its ratio to morphine from six representative monomers. Furthermore, the dummy template was creatively screened by semi-flexible docking method from opioid drugs. The system of dihydrocodeine (DI) as dummy template, methacrylamide (MAC) as founctional monomer, ethyleneglycol dimethacrylate (EGDMA) as crosslinker was chosen for MO imprinting. The morphological and magnetic properties of MMIP were characterized by FT-IR, TEM and VSM. The results suggested that molecularly imprinted polymer (MIP) was synthesized evenly on Fe 3 O 4 surface. The adsorption experiments revealed that MMIP showed better extraction capacity and selectivity toward MO and its analogues than the non-imprinted polymer (NIP). The MMIP possessed adsorption capacity of 14.71 mg/g for MO and the imprinting factor was 2.10 at separate adsorption and 1.87 at competitive adsorption. A magnetic molecularly imprinted solid phase extraction coupled with HPLC method (M-MISPE-HPLC) has been established for the analysis of MO in urine sample. The developed method was validated for its linearity (0.038–100 mg L −1 R 2 = 0.9937), precision (1.07%–3.72%) and accuracy (83.62%–100.37%).
研究领域:
分析化学
·
药物分析
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BEHAVIOURAL BRAIN RESEARCH
·
2012
·
IF 3.417
Haichen Niu,Yingwei Zheng,Joshua D. Rizak,Yaodong Fan,Wei Huang,Yuanye Ma,Hao Lei
Animals attain information about their environment through different sense organs. For example, the dominant external resource about the environment for rodents is obtained through olfaction. Many environmental conditions (stress or enriched environment) are known to affect an animal's susceptibility to drug addiction. However, it is not known how external information is integrated and paired with drug stimuli to develop into addictive behavior. Here, we investigated the effects of olfactory epithelium lesions induced with ZnSO4 effusion (ZnE) on morphine-induced sensitization and conditioned place preference in mice. We found that the lesion of the olfactory epithelium attenuated the repeated morphine (40 mg/kg)-induced behavioral sensitization and morphine-induced conditioned place preference (CPP) behaviors, such as hyper-locomotion during morphine (40 mg/kg) conditioned training. Additionally, the expression of FosB-like proteins, transcription factors associated with behavioral alterations, in the nucleus accumbens of the brain was attenuated in morphine administered mice treated by ZnE. Taken together, these results indicated that lesion of the olfactory epithelium lead to a decrease in morphine sensitization and CPP behavior in mice as well as modulate specific molecular markers of neuroadaption to drugs of abuse. These findings also suggest that olfaction plays an important role in the development of addictive behaviors that can be modulated by external actions.
研究领域:
神经科学
·
药理学
·
行为学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Yaqin Han,Jiajing Zhang,Junan Fang,Weizhen Xia,Jia An,Yufei Liu
To ensure public health and safety, it is crucial to develop highly sensitive, rapid, and visually interpretable platforms for detecting prohibited drugs and psychoactive substances. This study presents an innovative multimodal fluorescence immunosensor (MSN-MAb-AuNCs) based on a gated nanostructure, using morphine as a model antigen for trace detection. The platform employs mesoporous silica nanoparticles (MSN) for internal loading and external coupling of signaling molecules. A competitive immunoassay mechanism precisely activates the gated system, while fluorescence resonance energy transfer (FRET) ensures efficient signal transduction. A key innovation is the use of polyethyleneimine (PEI) as a "pseudo-hapten", which enhances the fluorescence of gold nanoclusters (AuNCs) and prevents premature FRET through its long-chain structure. This design enables accurate gating responses and seamless integration of multimodal detection. The immunosensor achieves an ultralow detection limit of 0.086 ng/mL for morphine and supports a one-step protocol for both visual semi-quantification and precise quantification. Additionally, the versatility of the gated system allows adaptation to diverse analytes and detection modes, providing a robust strategy for rapid and visual trace substance detection. By overcoming limitations of conventional methods, this study makes a significant contribution to advancing biosensing and analytical chemistry.
研究领域:
分析化学
·
生物传感
·
药物检测
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Guopan Sun,Shanfu Sun,Yinglin Wang,Xidong Hao,Yue Hao,Chao Liu,Caili Gong,Qianyong Yang,Weian Du,Pengfei Cheng
Developing efficient drug-detecting technologies is critical to the fight against their related abuse and trafficking crimes. In the present work, a novel morphine detection device based on metal-oxide-semiconductor (MOS) gas sensors with low-cost, highly sensitive, and portable is designed and fabricated. The exclusive sensitive material, ZnO nanoparticles self-assembly porous networks (ZnO-N) with ample oxygen vacancies (O V ) defects prepared by PVP-chelated precipitation followed by a chemical blowing calcination method, is decisive to morphine drug identification. O V defects can modulate the intrinsic electronic structure and endow ZnO-N with more suitable band structures for enhancing oxygen species adsorption and morphine molecules oxidation. The optimal ZnO-N-2 sensor exhibits positive linear relationship response values of 1.1–8.8 in the concentrations of 2–200 ppm toward the standard morphine drug sample at the optimal operating temperature of 250°C. More importantly, the homemade portable device equipped with the ZnO-N-2 sensor can accurately identify the morphine drug and perform early warning. This work innovatively demonstrates the feasibility of MOS gas sensors for the detection of stashing morphine drugs in methanol, which is of great significance for effectively combating global drug-related crimes.
研究领域:
材料科学
·
药物检测技术
·
传感器技术
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）