JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2024
·
IF 5.7
Yihan Wang,Jie Zheng,Yubin Xue,Bo Yu
Dipicolinic acid (DPA), a cyclic diacid, has garnered significant interest due to its potential applications in antimicrobial agents, antioxidants, chelating reagents, and polymer precursors. However, its natural bioproduction is limited since DPA is only accumulated in Bacillus and Clostridium species during sporulation. Thus, heterologous production by engineered strains is of paramount importance for developing a sustainable biological route for DPA production. Pseudomonas putida KT2440 has emerged as a promising host for the production of various chemicals thanks to its robustness, metabolic versatility, and genetic tractability. The dominant Entner–Doudoroff (ED) pathway for glucose metabolism in this strain offers an ideal route for DPA production due to the advantage of NADPH generation and the naturally balanced flux between glyceraldehyde-3-phosphate and pyruvate, which are both precursors for DPA synthesis. In this study, DPA production via the ED pathway was in silico designed in P. putida KT2440. The systematically engineered strain produced dipicolinate with a titer of 11.72 g/L from glucose in a 5 L fermentor. This approach not only provides a sustainable green route for DPA production but also expands our understanding of the metabolic potential of the ED pathway in P. putida KT2440.
研究领域:
微生物学
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生物化学
·
代谢工程
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DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Yue-Yang Ma,Zi-Jian Wang,Dong-Jin Qian
Detection of anthrax biomarker dipicolinic acid (DPA) is of great importance upon the crisis of bioterrorism. Development of fluorescent materials for DPA detection, particularly one that fully depends on single luminescent response, faces the challenge of being susceptible to interferences. The accompanying accuracy problems offer great opportunities for the establishment of more reliable ratiometric analysis method. Herein, a ratiometric fluorescent probe based on terbium functionalized graphitic carbon nitride nanosheets (Tb-g-C 3 N 4 NS) is attempted for quantitative detection of DPA to address the distinct function of g-C 3 N 4 NS as both carrier and reference fluorophore, which is a so-far unexplored option in fluorescent detection approaches. We achieve the incorporation of Tb 3+ into framework of g-C 3 N 4 NS by using a simple synthetic strategy comprised of thermal pyrolysis and ultrasonic exfoliation. Combining the reference signal over g-C 3 N 4 NS at 440 nm (I 440 ) with the response signal of Tb 3+ at 546 nm (I 546 ), concentration of DPA can be easily calculated via its linear correlation with the intensity ratio (I 546 /I 440 ), giving a precise measurement towards DPA with a detection limit as low as 9.9 nM. Besides enabling an excellent self-calibrating detection of DPA, this work also inspires broader use of g-C 3 N 4 NS for relevant process.
研究领域:
生物化学
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分析化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
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2021
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IF 4.32
Yang Mu,Fuyin Zhang,Ning Li,Shanshan Pi,Ang Li,Qing Hong,Jian He,Jiguo Qiu,Jiandong Jiang
Dipicolinic acid (DPA) is a widely distributed pyridine derivative containing two carboxylate groups. However, the catabolism of DPA mediated by microorganisms are rarely reported. Alcaligenes faecalis strain JQ135, previously isolated in our lab, was able to degrade various pyridine derivatives, including nicotinic acid (NA). In this study, it was found that strain JQ135 could utilize DPA as the sole carbon and nitrogen source for growth and degrade 1.8 mM (300 mg/L) DPA to a non-detectable level within 12 h. The catabolism of DPA by strain JQ135 extracted two intermediates, i.e., 3-hydroxy-2,6-dipicolinic acid and 4-imino-pent-2-enedioic acid. Moreover, deletion of the key genes essential for NA catabolism (such as moaCDEBmoeA and maiA ) did not affect DPA catabolism, indicating that the maleamate pathway and molybdenum cofactor are not involved in the DPA catabolism by strain JQ135. This study will provide insights into the bacterial catabolism of DPA.
研究领域:
微生物学
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环境科学
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生物化学
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产品关联
DOI
高影响因子
Advanced Composites and Hybrid Materials
·
2025
·Vol. 8
·期 3
·1-11
·
IF 21.8
Lin Xiaodong,Cao Zhengyang,Zeng Shiyu,Zhu Haoyu,Zhai Kairui,Yin Binfeng,Zhang Cheng,Peng Tao,Cheng Teng,Zhang Biao
In this work, we developed a dual-mode portable device that integrated a 3D-printed microfluidic chip for detection of dipicolinic acid (DPA) on chip. The system uses a ratiometric fluorescence nanoprobe formed by embedding carbon dots (CDs) into an Eu 3 ⁺ metal–organic framework (Eu-MOF). Upon reaction with DPA in the microchannel, red fluorescence was enhanced and blue fluorescence suppressed, enabling sensitive ratiometric detection of DPA on chip with a detection limit (LOD) of 0.04 µM. Interestingly, the composite Eu MOF /CDs/DPA also exhibits peroxidase-like activity, catalyzing the oxidation of TMB into a blue-colored product (oxTMB), which allows for colorimetric detection with an LOD of 10.14 µM. To improve usability and reduce environmental or instrumental variability, incorporating a microfluidic chip into a semi-portable device and utilizing a smartphone, making the system portable and miniaturized for easy operation. In the smartphone-assisted mode, the LODs were 0.33 µM (ratiometric fluorescence) and 12.27 µM (colorimetry), determined by RGB signal analysis, respectively. Moreover, satisfactory recoveries (85–104.6%) were achieved in the spiked real samples. Overall, this platform offers a straightforward, cost-effective, and versatile approach for DPA detection, with promising applications in food safety, environmental monitoring, and clinical diagnostics.
研究领域:
分析化学
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生物医学工程
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环境科学
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产品关联
DOI
高影响因子
Biomolecules
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2020
·Vol. 10
·期 3
·398
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IF 4.082
Shan Shan Qi,Hong Xing Zheng,Hai Jiang,Li Ping Yuan,Le Chen Dong
Diabetic nephropathy (DN) is one of the most important complications of diabetes, and the leading cause of end-stage renal disease (ESRD). While Chromium picolinate (CrPic) supplementation has been found to be effective in treating diabetes, its effects on diabetic-induced nephropathy have not been studied. Therefore, in this study, CrPic (1 mg kg−1d−1) was administered to a DN rat model by oral gavage for eight weeks to investigate its effects. The results show that CrPic supplementation caused a decrease in levels of blood glucose, serum insulin, blood urea nitrogen (BUN), serum creatinine, and urinary albumin in DN rats. It also reversed renal pathological changes, including renal glomerular sclerosis and interstitial fibrosis. In addition, the oxidative defense system in the kidneys of DN rats was found to be improved; the biological activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX) increased; and the content of malondialdehyde (MDA) lowered. Immunohistochemical results reveal that the expression levels of renal transforming growth factor-β1 (TGF-β1), Smad 2, and Smad 3 decreased significantly in the kidneys of rats in the CrPic-treated group. CrPic administration was thus found to ameliorate diabetic nephropathy in SD rats via an antioxidative stress mechanism, as well the ability to inhibit TGF-β1/Smad2/3 expression. This study suggests that CrPic could be a potential renal-protective nutrient against diabetic nephropathy.Keywords:diabetic nephropathy;chromium picolinate;renal fibrosis;antioxidative stress
研究领域:
内分泌学
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肾病学
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药理学
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产品关联
DOI
PubMed
高影响因子
Journal of Energy Storage
·
2025
·
IF 9.8
Huaiyu Wang,Yanjun Cai,Zhiran Zhang,Yu Liu,Rui Zhang,Ruiqiang Huo,Ruiyao Wu,Huiyun Cheng,Jiao Yin,Jian Yin,Zhi Su,Hui Zhu
Zinc-ion hybrid supercapacitors (ZHSCs) not only combines the advantages of batteries and supercapacitors, but also features low cost and environmental friendliness. However, the poor active site density and sluggish diffusion kinetics of carbon cathode brings about low capacity and terrible rate capability of ZHSCs. In this work, an activation assisted template procedure is proposed to synthesize nitrogen and phosphorous dual-doped hierarchical porous carbon (NP-HPC), where phytic acid acts as both doing precursor and activation medium. Benefitting from the synergetic modulation in porosities and surface functionalities, the optimized NP-HPC-2 delivers high capacity (394.51 F g −1 at 0.2 A g −1 ), superior rate capability (200.21 F g −1 at 50 A g −1 ) and excellent durability (95.7 % retention over 10,000 cycles). Consequently, the assembled ZHSC exhibits a remarkable energy density of 140 Wh kg −1 and an impressive power density of 40 kW kg −1 . Density functional theory (DFT) calculations revealed that the introduction of P doping adjust the distribution of local charge density of edged N with a lowered adsorption energy barrier for Zn 2+ storage. This work not only sheds light on the integrative effect of structure modulation and functionality regulation but also provide a feasible solution for the preparation of carbon cathodes towards high energy and power density ZHSCs.
研究领域:
材料科学
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能源存储
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电化学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2018
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IF 6.434
Peipei Yang,Qi Liu,Jingyuan Liu,Rongrong Chen,Rumin Li,Xuefeng Bai,Jun Wang
A novel phosphonate assisted fabrication dendritic fibrous nanosilica (DFNS)-based adsorbing material was successfully synthesis via organic modification with 3-aminopropyltriethoxysilane (KH550), epichlorohydrin (ECH) and phytic acid (PA) on the grounds of hard-soft-acid-base theory, in which organic phosphorous can be applied for efficient chelating uranium(VI) (U(VI)). The adsorption properties can be evaluated by setting a series parameters (pH, adsorbent dose, contact time, initial U(VI) concentration). It is clear that uranium as a “hard” lewis acid can easily form chelating bond with “hard” donor-ligands so that as-papered PA-DFNS has an excellent adsorption capacity (q m = 1106 mg g −1 , 298.15 K), which exhibits a 163% increment compared with that of original DNFS under the same condition. In the meantime, the adsorption equilibrium time of PA-DNFS (t = 60 min) was shortened by 33% compared with that of pristine DNFS. Besides, the PA-DFNS exhibited good removal efficiency and stability under the 0.1 M HNO 3 after 6 cycles, extending the application of PA-DFNS in the field of adsorption.
研究领域:
材料科学
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环境科学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 12
·1-9
·
IF 6.232
Yang Dan,Mei Shiliang,Wen Zhuoqi,Wei Xian,Cui Zhongjie,Yang Bobo,Wei Chang,Qiu Yi,Li Min,Li Hui,Zhang Wanlu,Xie Fengxian,Wang Le,Guo Ruiqian
Dipicolinic acid (DPA) is employed as a significant biomarker to detect Bacillus anthracis , which can do serious damages to the health of human beings. Hence, it is crucial to develop a fast and highly efficient strategy for DPA monitoring. In this work, based on silicon nanoparticles (Si NPs) and terbium metal-organic frameworks (Tb-MOFs), a hybrid structure (Si NPs/Tb-MOFs) as a novel dual-emitting fluorescence probe was fabricated for ratiometric detection of DPA, where blue light-emitting Si NPs (Ex: 280 nm; Em: 422 nm) are encapsulated into green light-emitting Tb-MOFs (Ex: 280 nm; Em: 547 nm). The optical properties and chemical composition of the as-obtained Si NPs/Tb-MOFs were characterized in detail. The Si NPs/Tb-MOFs probe not merely possesses the merits of a facile synthesis method but also is an excellent fluorescence probe. The response time towards DPA is less than 30 s, revealing that the process of detecting DPA can be completed in such a short time. The limit of detection for DPA is 5.3 nM, which is four orders of magnitude lower than an infectious dosage of anthrax spores for human beings (60 μM). This dual-emitting Si NPs/Tb-MOFs probe with interference-free and self-calibrating properties may be a potential candidate for further development in medical diagnosis.
Graphical abstract
研究领域:
生物化学
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医学诊断
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材料科学
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2020
·
IF 5.339
Qiuju Zhou,Yajun Fang,Jiayu Li,Dan Hong,Panpan Zhu,Shihong Chen,Kejun Tan
In this work, a novel dual-ratiometric optical probe based on europium-doped carbon dots (Eu-CDs) was developed for colorimetric and fluorescent visual sensing dipicolinic acid (DPA), a anthrax biomarker. Eu-CDs with recessive signals were prepared via thermal pyrolysis method. And the magenta appears when Eu-CDs coordinates with Eriochrome Black T (EBT). Based on the ligand displacement strategy, DPA reduces magenta and makes blue appear, which develop ratiometric colorimetric visual detection methods of DPA. Under 270 nm excitation, DPA make the red fluorescence signal of Eu in EBT-CDs@Eu appear, based on the absorbance-energy transfer-emission effect, the ratiometric fluorescent visual detection assay of DPA has been developed. Based above, dual-ratiometric optical probe was designed successfully. The limit of detection (LOD) is 10.6 nM for ratio colorimetric assay . More prominently, naked-eye determination of DPA without instrument is as low as 1.0 μM. Furthermore, its practicability was verified by quantifying the DPA in Bacillus subtilis spores and human urine (with RSD≤2.35%). The sensor shows great superiority in on-site analysis of DPA, especially in limited instruments conditions.
研究领域:
分析化学
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生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FUEL
·
2021
·
IF 6.609
Guokai Cui,Shuzhen Lyu,Huiyong Wang,Zhiyong Li,Ruina Zhang,Jianji Wang
A series of pyridinolate anion-functional ionic liquids (ILs) containing different kinds of cations were designed and prepared, including tri- n -hexyltetradecylphosphonium [P 66614 ], tri- n -butylethylphosphonium [P 4442 ], and 1,1,3,3-tetramethylguandinium [TMG] cations and pyridinolate [PyO] anions with different N positions. Densities and viscosities at different temperatures were measured because of their importance for the absorption of SO 2 . Effect of different structures on the absorption of SO 2 was studied, including the type and the alkyl chain length of cations, and the N positions of [PyO] anions (2-, 3-, and 4-). Additionally, effects of SO 2 concentration and temperature for absorption were investigated. Especially, up to 6.00 and 2.53 mol SO 2 per mole IL could be obtained by [P 66614 ][4-PyO] at 1 bar and 0.1 bar of SO 2 , respectively, when absorptions performed at 20 °C. The results indicate that (1) the aprotic cations weakened the cation⋯anion interaction and resulted in the released active sites on the anions, and (2) N position has an influence on charge distribution of [PyO] anions and resulted in the tunable absorption of SO 2 . Reversible SO 2 absorption and [P 66614 ][4-PyO] regeneration could be performed several times without losing efficiency. Furthermore, the mechanism of absorption was studied through FT-IR and NMR methods and the results showed that the absorption of SO 2 by [P 66614 ][4-PyO] includes physisorption and chemisorption.
研究领域:
化学工程
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环境科学
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材料科学
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产品关联
DOI
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2021
·Vol. 40
·期 3
·337-342
·
IF 6
Xiaoxia Le,Hui Shang,Shirong Gu,Guangqiang Yin,Fuqing Shan,Danyang Li,Tao Chen
Comprehensive Summary Smart fluorescent patterns enable dynamic color variation under external stimuli, showing a much higher security level in the field of anti-counterfeiting. However, there is still lacking of a simple and convenient way to achieve dynamic fluorescence changes. Herein, a fluorescent organohydrogel made up of a poly( N , N -dimethylacrylamide- co -isopropylacrylamide) (p(DMA-NIPAM)) hydrogel network and a poly(lauryl methacrylate) (PLMA) organogel network was fabricated via a two-step interpenetrating technique. The former network bears naphthalimide moieties (DEAN, green fluorescent monomer) and the later contains 6-acrylamidopicolinic acid (6APA, fluorescent ligand), leading to emitting green fluorescence. When Eu 3+ was introduced and coordinated with 6APA, the organohydrogel displays red fluorescence, which can further emit yellow after applying thermal stimulus. Furthermore, by adjusting the proportion of comonomers, various organohydrogels can be obtained, which can be programmed and act as an effective platform for the encryption and decryption of secret information.
研究领域:
材料科学
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化学
·
防伪技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）