Food & Function
·
2023
·Vol. 14
·期 9
·4228-4241
·
IF 6.1
Tingting Li,Like Lin,Cong Li,Jingyi Zheng,Bang Chen,Yehua Shen,Difeng Ren
In this work, RLWPF (Arg-Leu-Trp-Pro-Phe) and VLRLF (Val-Leu-Arg-Leu-Phe) were investigated for the effects against D-galactose (D-gal) induced cognitive impairment by modulating the gut microbiota composition. The effects on serum metabolite production were further investigated. The two novel peptides derived from walnut protein alkaline protease hydrolysates were predicted by docking to acetylcholinesterase (AChE) with the highest binding affinities, −10.3 and −9.9 kcal mol−1, considered as the potential neuroprotective peptides. In behavioral experiments, RLWPF and VLRLF treatment significantly restored spatial learning and memory impairment induced by D-gal. The results showed that RLWPF and VLRLF could alleviate cholinergic dysfunction, oxidative stress, and inflammation to varying degrees caused by D-gal-induced aging. Furthermore, 16S rRNA analysis revealed that RLWPF and VLRLF treatment improved cognitive impairment by regulating the composition of the gut microbiota and the abundance of harmful bacteria, including the ratio of Firmicutes to Bacteroidetes, Helicobacter, Allobaculum, Alistipes, Mucispirillum, and Odoribacter. In addition to the same regulation, RLWPF and VLRLF had their marker and regulatory flora. Studies based on the gut microbiota would allow a better understanding of the neuroprotective effects of walnut-derived peptides, supporting that walnut-derived peptides could be potential functional ingredients in foods and nutraceuticals or drug candidates in the treatment of cognitive dysfunction.
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Drug Testing and Analysis
·
2020
·Vol. 13
·期 3
·720-728
·
IF 2.903
Cui-Mei Liu,Hong-Yuan He,Lin Xu,Zhen-Dong Hua
Performing fast qualitative identification of seized illegal drugs by Raman spectroscopy is challenging due to fluorescence interference as well as chemical complexity. Spectrometers with 785-nm excitation, 1,064-nm excitation, and sequentially shifted excitation (SSE) were compared for their effect on fluorescence reduction. The characteristic peaks method, which is independent of cutting agents, was tested as a new strategy to broaden the application of the Raman technique. The suitability of the characteristic peaks method was fully examined by analyzing a large amount of seized illegal drugs, including 72 methamphetamine hydrochloride (concentration range of 13.9%–99.4%), 68 ketamine hydrochloride (17.7%–99.8%), 176 heroin hydrochloride (5.2%–79.5%), 51 cocaine hydrochloride (21.1%–94.5%), and 33 cocaine base (30.9%–92.5%) samples. The results showed that seized methamphetamine, ketamine, and cocaine samples had no or little fluorescence. Hence, in regard to detection of these samples, the advantage of using 1,064-nm excitation and SSE compared with 785-nm excitation was quite limited. Regarding the heroin samples, a significant improvement of the “high” confident positive detected rate was evident for 1,064 nm excitation (60.8%) and SSE (61.4%), compared with 785-nm excitation (13.1%). However, it was also seen that even if 1,064-nm excitation and SSE were applied, the fluorescence of heroin samples was still unable to be fully overcome. By using the characteristic peaks method, low LOD results of 5%–20% were acquired for 40 types of drug mixtures, and lower LODs were obtained for the 60% of the drug mixtures compared with library searching method. Raman spectroscopy in conjunction with the characteristic peaks method was shown to be fast, simple, accurate, and sensitive in the qualitative analysis of seized drug samples.
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Journal of Functional Foods
·
2020
·
IF 3.701
Xiaomeng Xu,Rong Liang,Dongmei Li,Chengyao Jiang,Songyi Lin
To investigate the dose-effect relationship of sea cucumber peptides on memory function improvement, animal experiments, behavioral experiments, ACh content, AChE activity, hippocampal morphology, and protein modifications were detected. Sea cucumber peptides (650 mg/kg, 1300 mg/kg, 2600 mg/kg body weight) and piracetam (400 mg/kg) were gavaged into the mice. Scopolamine (5 mg/kg) were administered intraperitoneally to mice. In behavioral experiments, the escape latency and the shocked time of sea cucumber peptides (SCP) groups were significantly reduced ( P < 0.05). Determination of biochemical indicators found that ACh content and AChE activity of SCP groups were increased ( P < 0.05). The number of neurons in the hippocampus of SCP groups were larger, arranged neatly and intact. The histone acetyltransferases activity of SCP groups may be lower than that of the Scopolamine group, so the acetylation level is down-regulated and approached to dynamic equilibrium, thereby the memory activity of mice was improved.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Caiyun Li,Dingwei Zheng,Tianle Zhang,Siyuan Qian,Xinyao Liu,Zhengfu Yang,Kean-Jin Lim,Weihong Min,Jianqin Huang,Yiyi Yang,Zhengjia Wang
Cognitive decline associated with aging and neuroinflammation has been linked to gut microbiota dysbiosis and systemic inflammation, potentially mediated through the gut–brain axis. Bioactive peptides have shown potential as therapeutic candidates for neurodegenerative and neuroinflammatory disorders. While the walnut-derived peptide EVSGPGYSPN (EV-10) has demonstrated cognitive benefits, the therapeutic potential of its recombinant form, recombinant walnut-derived peptide (rWDP), expressed and purified from an Escherichia coli system, remains to be fully characterized. In this study, behavioral assessment using the Morris water maze revealed that rWDP significantly improved spatial learning and memory in d-galactose-induced aging mice. rWDP treatment was associated with changes in gut microbiota composition, including an increase in Akkermansia muciniphila and improvements in colon mucosal integrity. Systemic inflammation appeared to be attenuated, with reduced levels of IFN-γ and VCAM-1 and normalization of G-CSF and CXCL1 levels. In the brain, rWDP was associated with reduced microglial activation and preservation of hippocampal neuronal architecture. Notably, decreased p21 expression was observed in the hippocampus and cortex. In vitro, rWDP suppressed LPS-induced nitric oxide production and attenuated the expression of proinflammatory genes in BV2 microglial cells. Metabolomic analysis suggested a restoration of neurotransmitter homeostasis following rWDP treatment, with increased levels of 5-hydroxyindoleacetic acid, kynurenine, and glutathione observed. Overall, rWDP may alleviate cognitive deficits through multifaceted pathways involving the gut–brain axis and immune modulation, warranting further mechanistic investigation.
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