JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
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2020
·Vol. 20
·期 9
·5305-5312
·
IF 1.134
Wang Zian,Liu Yang,Jiang Yifei,Wang Peng,Ji Min
Although the preparation of Indocyanine Green (ICG) liposomes obtained stronger performance than free ICG. With increase in depth of tissue, ICG exhibits limited background (SBR) and blurs structure characteristics. In this research, a Stearylamine-Bearing cationic liposome was prepared
for improved fluorescence performance (higher SBR and deeper imaging depth). In addition, the effect of ICG and lipid interactions was explored. Hyaluronic acid is subsequently modified on the liposomes for prolonging blood circulation time and active tumor targeting. In vitro study
confirmed that the liposome (HA-ICG-SA-LP) was capable of reversing surface zeta potential under acidic conditions in the presence of HAase which might enhance cellular uptake. Additionally, the photothermal heating of liposomes was investigated. The MTT assay showed that the liposome has
strong cancer cell inhibition ability. In summary, HA-ICG-SA-LP exhibited a great potential for high sensitivity imaging and tumor hyperthermia.
研究领域:
药剂学
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肿瘤学
·
生物医学工程
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DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
·
2025
·
IF 12.6
Kaiming He,Desheng Chen,Dongzi Zhu,Wenjie Zheng,Lei Lyu,Mingshen Zhang,Zeping Chen,Xiaowen Wang,Yongwei Hu,Binsheng Fu
Hepatocellular carcinoma (HCC) poses a significant therapeutic challenge, driving the need for novel treatment strategies. This study investigates the combination of photothermal therapy (PTT) and metalloimmunotherapy for HCC treatment using Co + diABZI@J-dICG nanoparticles. Indocyanine green (ICG), an FDA-approved near-infrared (NIR) dye, is dimerized into J-aggregates to enhance PTT by improving light absorption and photothermal efficiency. The cGAS-STING pathway, a key mediator of innate immunity, is activated by the STING agonist diABZI, while cobalt ions (Co 2+ ) further enhance immune responses. The Co + diABZI@J-dICG nanoparticles take advantage of ICG’s hepatotropic properties for sustained tumor accumulation and immune activation, resulting in significant tumor growth inhibition and reduced HCC recurrence following hepatectomy.
研究领域:
肿瘤学
·
免疫学
·
纳米医学
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产品关联
DOI
PubMed
高影响因子
Journal of Pharmaceutical Analysis
·
2013
·
IF 0
Zheng-Jun Cheng,Hong-Mei Zhao,Qian-Yong Xu,Rong Liu
The binding characteristics of indigotin with human serum albumin (HSA) and bovine serum albumin (BSA) have been investigated by various spectroscopic techniques. Spectroscopic analysis revealed that the quenching mechanism between indigotin and HSA/BSA belonged to the static quenching. The displacement experiments suggested that indigotin primarily bound to tryptophan residues on proteins within site I. The thermodynamic parameters indicated that the binding of indigotin–HSA/BSA mainly depended on the hydrophobic interaction. The binding distance of indigotin to HSA/BSA was evaluated. The results by synchronous fluorescence, three-dimensional fluorescence, Fourier Transform Infrared spectroscopy (FT-IR) and circular dichroism (CD) spectra showed that the conformation of proteins altered in the presence of indigotin.
研究领域:
生物化学
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药物化学
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分子生物学
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产品关联
DOI
PubMed
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
·
2021
·Vol. 49
·期 8
·2605-2617
·
IF 9.236
Zhang Yang,Cheng Hongwei,Chen Hu,Xu Peiyao,Ren En,Jiang Yonghe,Li Dengfeng,Gao Xing,Zheng Yating,He Pan,Lin Huirong,Chen Biaoqi,Lin Gan,Chen Aizheng,Chu Chengchao,Mao Jingsong,Liu Gang
Purpose To surmount the critical issues of indocyanine green (ICG), and thus achieving a precise surgical navigation of primary liver cancer after long-term transcatheter arterial embolization. Methods In this study, a facile and green pure-nanomedicine formulation technology is developed to construct carrier-free indocyanine green nanoparticles (nanoICG), and which subsequently dispersed into lipiodol via a super-stable homogeneous lipiodol formulation technology (SHIFT nanoICG) for transcatheter arterial embolization combined near-infrared fluorescence-guided precise hepatectomy. Results SHIFT nanoICG integrates excellent anti-photobleaching capacity, great optical imaging property, and specific tumoral deposition to recognize tumor regions, featuring entire-process enduring fluorescent-guided precise hepatectomy, especially in resection of the indiscoverable satellite lesions (0.6 mm × 0.4 mm) in rabbit bearing VX2 orthotopic hepatocellular carcinoma models. Conclusion Such a simple and effective strategy provides a promising avenue to address the clinical issue of clinical hepatectomy and has excellent potential for a translational pipeline.
研究领域:
肿瘤学
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生物医学工程
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影像医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CONTROLLED RELEASE
·
2020
·
IF 7.727
Hu Chen,Hongwei Cheng,Qixuan Dai,Yi Cheng,Yang Zhang,Dengfeng Li,Yang Sun,Jingsong Mao,Ke Ren,Chengchao Chu,Gang Liu
Accurate identification of surgical margins for malignancy remains a challenge in the surgical therapy of cancer, and this encountered interoperative difficulties which directly contribute to the prognosis of patients. In recent years, indocyanine green (ICG) has been approved and applied in clinical settings for lesions detection, especially for the precise surgical resection. However, rapid clearance and poor stability greatly limit its clinical practicality. Herein, a super-stable homogeneous iodinated formulation technology (SHIFT) is designed to realize sufficient dispersion of ICG into lipiodol (SHIFTs) for transcatheter embolization (TAE) synergistic fluorescence-guided resection. Particularly, SHIFTs is prepared in a green physical mixture via a carrier-free manner, which possesses controlled morphology, long-term stability, and improved optical characteristics of ICG (fluorescence/photoacoustic/photothermal activities). Furthermore, the viscosity of the synthetic solvent is comparable to lipiodol, and further assessment demonstrated the same efficacy in computed tomography . The performance of SHIFTs in the fluorescence navigation was further evaluated in vivo by TAE therapy to the rabbit VX2 tumor model for a two-week monitor. The integration of near-infrared fluorescence surgery navigation and TAE could effectively guarantee the precise resection for hepatocellular carcinoma . This SHIFT system provides good potentials for ameliorating the dilemma of precise fluorescent navigation for surgical resection after arterial embolization in clinical practice.
研究领域:
医学影像学
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肿瘤学
·
外科手术
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DOI
PubMed
高影响因子
Stem Cells International
·
2018
·
IF 3.989
Yong-Heng Luo,Juan Chen,En-Hua Xiao,Qiu-Yun Li,Yong-Mei Luo
Demethylating agent zebularine is reported to be capable of inducing differentiation of stem cells by activation of methylated genes, though its function in hepatocyte differentiation is unclear. p38 signal pathway is involved in differentiation of hepatocytes and regulating of DNA methyltransferases 1 (DNMT1) expression. However, little is known about the impact of zebularine on bone marrow mesenchymal stem cells (BMMSCs) and p38 signaling during hepatic differentiation. The present study investigated the effects of zebularine on hepatic differentiation of rabbit BMMSCs, as well as the role of p38 on DNMT1 and hepatic differentiation, with the aim of developing a novel strategy for improving derivation of hepatocytes. BMMSCs were treated with zebularine at concentrations of 10, 20, 50, and 100 μM in the presence of hepatocyte growth factor; changes in the levels of hepatic-specific alpha-fetoprotein and albumin were detected and determined by RT-PCR, WB, and immunofluorescence staining. Expression of DNMT1 and phosphorylated p38 as well as urea production and ICG metabolism was also analyzed. Zebularine at concentrations of 10, 20, and 50 μM could not affect cell viability after 48 h. Zebularine treatment leads to an inhibition of DNMT activity and increase of hepatic-specific proteins alpha-fetoprotein and albumin in BMMSCs in vitro; zebularine addition also induced expression of urea production of and ICG metabolism. p38 signal was activated in BMMSCs simulated with HGF; inhibition of p38 facilitated the synthesis of DNMT1 and albumin in cells. Zebularine restrained DNMT1 and phosphorylated p38 which were induced by HGF. Therefore, this study demonstrated that treatment with zebularine exhibited terminal hepatic differentiation of BMMSCs in vitro in association with hepatocyte growth factor; p38 pathway at least partially participates in zebularine-induced hepatic differentiation of rabbit BMMSCs.
研究领域:
干细胞研究
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分子生物学
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细胞信号传导
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表观遗传学
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DOI
高影响因子
危险品化学品经营许可证（带存储）