RSC Advances
·
2021
·Vol. 11
·期 50
·31601-31607
·
IF 3.361
Bi-Xian Zhang,Pei-Shan Li,Ying-Ying Wang,Jia-Jun Wang,Xiu-Lin Liu,Xue-Yang Wang,Xiao-Mei Hu
Indole-3-acetic acid (IAA) plays an important role in the growth and development of plants. In this study, a series of predominant strains were isolated and identified as Enterobacter sp. with remarkable IAA-producing capabilities. The IAA-producing strains are mainly tryptophan-dependent and have significantly high yields of IAA (3477 μg mL−1 and 3378 μg mL−1). The ipdC gene encoding indole-3-pyruvate decarboxylase was identified by genomic analysis and RT-qPCR analysis, indicating the involvement of the indole-3-pyruvic acid (IPyA) pathway of IAA biosynthesis. The IPyA pathway was also confirmed by the intermediate assay. The IAA product of microbial metabolites was isolated, purified and characterized. These microbes exhibiting IAA production significantly promoted the growth of maize, increasing root length, plant height, fresh weight and dry weight. Thus, Enterobacter sp. with high IAA production has great prospects in agricultural and industrial applications.
研究领域:
微生物学
·
植物学
·
农业科学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2019
·
IF 2.298
Rongrong Li,Shiyun Zhan,Gang Chen,Yanxian Jin,Binbin Yu,Jia Zhao,Deman Han,Huajun Fan
The solid–liquid solubility of indole-3-acetic acid in pure methanol, ethanol, n-propanol, isopropanol, acetone, n-butanol, acetonitrile, chloroform, ethyl acetate, 1,4-dioxane, N,N-dimethylformamide (DMF), and dimethyl sulfoxide (DMSO) was measured by using a isothermal saturation method. The determination temperature range is from 278.15 to 323.15 K. The order of solubility data from high to low was ethyl acetate > DMSO > DMF > n-butanol > acetone > isopropanol > 1,4-dioxane > n-propanol > ethanol > methanol > acetonitrile > chloroform. Two thermodynamic models (modified Apelblat equation and λh equation) were used to correlate the solubility values of indole-3-acetic acid in different monosolvents, and the two equations can correlate the experimental values very well. The values of root-mean-square deviations (RMSDs) and relative average deviations (RADs) between the experimental and calculated solubility values were not exceeding 0.27 × 10–6 and 0.75 × 10–2, respectively. In order to study the effect of solvation interaction on solubility, the solute–solvent and solvent–solvent interactions were discussed.
研究领域:
化学工程
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Microbiology
·
2021
·
IF 5.64
Zhao Shuxue,Chen Xi,Sun Qianshu,Wang Fei,Hu Chunhui,Guo Lizhong,Bai Jie,Yu Hao
Indole-3-acetic acid (IAA), known as a common plant hormone, is one of the most distributed indole derivatives in the environment, but the degradation mechanism and cellular response network to IAA degradation are still not very clear. The objective of this study was to elucidate the molecular mechanisms of IAA degradation at the protein level by a newly isolated strain Pseudomonas sp. LY1. Label-free quantitative proteomic analysis of strain LY1 cultivated with IAA or citrate/NH4Cl was applied. A total of 2,604 proteins were identified, and 227 proteins have differential abundances in the presence of IAA, including 97 highly abundant proteins and 130 less abundant proteins. Based on the proteomic analysis an IAA degrading (iad) gene cluster in strain LY1 containing IAA transformation genes (organized as iadHABICDEFG), genes of the β-ketoadipate pathway for catechol and protocatechuate degradation (catBCA and pcaABCDEF) were identified. The iadA, iadB, and iadE-disrupted mutants lost the ability to grow on IAA, which confirmed the role of the iad cluster in IAA degradation. Degradation intermediates were analyzed by HPLC, LC-MS, and GC-MS analysis. Proteomic analysis and identified products suggested that multiple degradation pathways existed in strain LY1. IAA was initially transformed to dioxindole-3-acetic acid, which was further transformed to isatin. Isatin was then transformed to isatinic acid or catechol. An in-depth data analysis suggested oxidative stress in strain LY1 during IAA degradation, and the abundance of a series of proteins was upregulated to respond to the stress, including reaction oxygen species (ROS) scavenging, protein repair, fatty acid synthesis, RNA protection, signal transduction, chemotaxis, and several membrane transporters. The findings firstly explained the adaptation mechanism of bacteria to IAA degradation.
研究领域:
微生物学
·
植物学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Algal Research-Biomass Biofuels and Bioproducts
·
2025
·
IF 4.5
Juan Liu,Shiqing Sun,Kangyu Chen,Shumei Gao,Dixing Chen,Yuejin Zhang,Changwei Hu
Fungi-microalgae consortia have great potential in microalgae harvesting and wastewater treatment. However, the symbiotic mechanisms underlying diffusible signaling metabolite production by fungi and microalgae remain unclear. Here, the positive impacts of Clonostachys rosea on Chlorella sp. were investigated using metabolomics and transcriptomics during a 5-day non-contact co-culture experiment. Co-cultured Chlorella sp. had a significantly higher growth rate and better photosynthetic performance than its mono-culture equivalent. The metabolites played important roles in the up-regulation of energy and amino acid metabolism processes in algal cells, whilst transcriptomics showed the up-regulation of genes involved in photosynthesis and signaling molecules. The biosynthesis of tryptophan and indole-3-acetic acid (IAA) was up-regulated in both algal cells and fungi, indicating that IAA is an important diffusible signaling molecule for algal growth regulation. This work provides novel insights into the signaling molecule interactions between fungi and microalgae in fungi-microalgae consortia.
研究领域:
微生物学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS
·
2019
·Vol. 19
·期 24
·5483
·
IF 3.031
Xiaodong Cao,Xueting Zhu,Shudong He,Xuan Xu,Yongkang Ye
A selective and sensitive electrochemical sensor was developed for simultaneous detection of phytohormones indole-3-acetic acid (IAA) and salicylic acid (SA). The sensing interface was fabricated on a porous, three-dimensional networked graphene hydrogel (GH) modified glassy carbon electrode (GCE). The electrocatalytic behavior of IAA and SA on the surface of the modified electrode (GH/GCE) was investigated by cyclic voltammetry and linear sweep voltammetry. Results show that the oxidation reactions of IAA and SA occur at different potentials, which enable their simultaneous detection at the sensing interface. Under optimal conditions, the GH/GCE exhibited good selectivity and stability and its response, unaffected by various interferents, was linear in the range of 4 to 200 μM of IAA and SA. The limit of detection (S/N= 3) achieved were 1.42 μM for IAA and 2.80 μM for SA. The sensor performance was validated by measuring for IAA and SA in real vegetable samples with satisfactory results.Keywords:electrochemical sensor;simultaneous detection;indole-3-acetic acid;salicylic acid;graphene hydrogel
研究领域:
植物学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
SCIENTIA HORTICULTURAE
·
2017
·
IF 1.624
Wenjing Lu,Jingxin Chen,Xingchen Ren,Jiajia Yuan,Xueyuan Han,Linchun Mao,Tiejin Ying,Zisheng Luo
MADS-box genes, which encode highly conserved DNA-binding transcriptional factors, can regulate developmental processes in plants. FaMADS1a has not been functionally analyzed in non-climacteric fruit to date. In this study, one strawberry ( Fragaria × ananassa ) MADS-box gene, FaMADS1a , was cloned and its tissue-specific expression profiles were analyzed. FaMADS1a expressed in petal and fruit, and its expression levels decreased significantly during fruit ripening. Multiple sequence alignments suggested that FaMADS1a protein exhibited conserved MADS_MEF2_like motif, K-box region, MADS domain, SRF-TF motif and ARG80 domain. Phylogenetic analysis revealed that FaMADS1a belonged to the SEP1/2 clade. Phytohormone abscisic acid (ABA) suppressed FaMADS1a expression but accelerated the ripening process and anthocyanin-related gene expressions, while indole-3-acetic acid (IAA) induced FaMADS1a expression with delayed ripening phenotype and reduced expressions of anthocyanin-related genes. The pBI121 vector-mediated over-expression of FaMADS1a reduced the expressions of four anthocyanin-related genes and delayed fruit ripening compared to the control. Results suggested that FaMADS1a played a negative role in anthocyanin accumulation of strawberry fruit via repressing FaPAL6 , FaCHS , FaDFR and FaANS .
研究领域:
植物生物学
·
分子遗传学
·
植物发育
✓ 已验证
产品关联
DOI
高影响因子
CHEMOSPHERE
·
2020
·
IF 5.778
Feili Li,Feng Yang,Yihao Chen,Hui Jin,Yaling Leng,Jiade Wang
Making municipal sludge into garden soil is a challenging issue in land using due to the high content of heavy metals, however phytoremediation can reduce the heavy metal pollution in the soil. Three artificial regulators were used in combination to improve phytoremediation of heavy metals by Bryophyllum laetivirens from municipal sludge made garden (MSMG) soil. Results showed that B. laetivirens grew well in MSMG soil and bioaccumulated Cu, Pb, Zn, Cd, and Ni by 2.16–11.0 times higher than those grew in local common garden soil. The application of ethylenediaminetetraacetic acid (EDTA), indole-3-acetic acid (IAA) and microbial liquid (BL) promoted the bioaccumulation of heavy metals of plants in MSMG soil, with 2.1–6.8 times than the control group. The optimum dose for the phytoremediation of B. laetivirens was the combining treatment of 3 mmol kg −1 EDTA, 10 −10 M IAA, and 5 ml kg −1 BL, which has been successfully applied in MSMG soil. EDTA treatment is more direct and effective in facilitating HM uptake of root, while the other two treatments play important roles in promoting the transport of HMs in plants.
研究领域:
环境科学
·
植物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 10
·1-9
·
IF 5.3
Cheng Ye,Tian Dong-Yang,Chen Cui-Li,Bao Ning,Wu Zeng-Qiang
Stainless steel sheets were coated with carbon ink to obtain disposable carbon electrodes, which were used as supports for moleculary imprinted polymer (MIP) electrochemical sensors by electropolymerizing o-phenylenediamine and o-aminophenol along with indole-3-acetic acid (IAA) as the template. After optimization, the MIP biosensors could be used for sensitive and selective detection of IAA with the limit of quantification of 0.1 µM. Our experimental results showed that stable and reproducible electrochemical responses could be achieved for the disposable MIP biosensors. This approach was successfully used for detection of IAA in different tissues of pea sprouts. This study reveals the potential of MIP electrochemical sensors in practical applications and shrinks the trench between the research and the real world. Graphical Abstract
研究领域:
分析化学
·
植物生理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
IONICS
·
2017
·Vol. 24
·期 8
·2465-2472
·
IF 2.062
Sun Junyong,Gan Tian,Zhai Rui,Fu Wanqiu,Zhang Miaomiao
In the current study, a simple, recoverable, and stable sensor was developed based on SiO 2 spheres functionalized with 6-nm Au nanoparticles (SiO 2 @AuNPs). Fabrication procedures and analytical application of this sensor toward quantitative determination of diuron in the presence of interfering compound indole-3-acetic acid were verified by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse voltammetry (DPV) methods. It has been observed that diuron oxidation at the surface of sensor occurred at a potential of about 1.09 V which was 210 mV higher than that of indole-3-acetic acid, confirming that this newly prepared sensor appeared to be a good platform for the selective detection of diuron. Moreover, DPV demonstrated a linear relationship from 0.20 to 55 μmol/L and a detection limit of 51.9 nmol/L for diuron. Finally, this sensor was utilized for the determination of diuron in complex vegetable samples.
研究领域:
分析化学
·
环境科学
·
植物生理学
✓ 已验证
产品关联
DOI
高影响因子
Food Analytical Methods
·
2017
·Vol. 11
·期 4
·1155-1162
·
IF 2.038
Zheng Yang,Peng Xiaofeng,Wu Yiwei
A sensitive method has been established by using activated carbon as an adsorbent for extraction of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) from bean sprout, tomato, potato, cucumber, and wheat grain prior to field-amplified sample injection-micellar electrokinetic capillary chromatography (FASI-MEKC) detection. Parameters affecting FASI and solid-phase extraction (SPE) were optimized in detail. Under the optimal conditions, the sensitivity of SPE-FASI-MEKC for IAA and IBA was respectively improved by 32.5 and 29.7 times as compared with the direct MEKC. The detection limits were 4.1 for IAA and 5.3 ng mL −1 for IBA with 4.9–5.2% RSD ( n = 9, C = 0.03 μg mL −1 ). The new method has been successfully applied to detect IAA and IBA in bean sprout, tomato, potato, cucumber, and wheat grain with the recoveries of 81.7–110%.
研究领域:
植物学
·
分析化学
✓ 已验证
DOI
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Jing Jiang,Chenyang Zhang,Meng Na,Shangqi Xu,Xiaoping Li,Shoubiao Zhou,Jihai Zhou
Soil cadmium (Cd) pollution has become increasingly severe, negatively affecting plant growth, soil safety, the ecological balance, and public health. Phytoremediation technology is an effective method for remediating soil Cd contamination because of its low cost, environmentally friendly nature, and minimal soil disturbance. However, phytoremediation efficiency is affected by factors such as the plant species, Cd concentration, and soil environment. In this study, a 180-day indoor potted experiment was conducted to explore the influences of various indole-3-acetic acid (IAA) levels on the growth, physiological and biochemical responses, Cd uptake, and soil enzyme activity of Cinnamomum (C.) camphora seedlings using 20 mg kg −1 Cd-contaminated soil with or without IAA treatments. The results showed that Cd stress significantly suppressed C. camphora seedling growth, whereas IAA application improved the basal diameter of seedlings by 22.22–133.33 %. This effect of IAA was observed primarily at low concentrations, while higher concentrations showed diminished effects. Compared to the Cd pollution treatment, adding 20 mg kg −1 IAA significantly enhanced the activities of antioxidant enzymes (SOD, POD, CAT), reduced the malondialdehyde (MDA) content, and decreased soil basal respiration (SBR). Moreover, IAA treatment promoted Cd uptake in the roots, stems, and leaves, and reduced the soil Cd content by 22.90–41.17 %. In conclusion, exogenous IAA addition effectively alleviates Cd stress by regulating the plant’s physiological and biochemical characteristics and soil enzyme activities. These findings suggest that the combined use of IAA and C. camphora represents a promising remediation method for soil heavy metal pollution.
研究领域:
植物学
·
植物生理学
·
环境科学
·
生物化学
·
土壤科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2020
·
IF 3.807
Zhaohong Su,Daili Tang,Junjie Liu,Xiaolan Yang,Siyu Xu,Wenjing Xu,Yuqing Zhou,Mingyang Xu,Jianlong Yi,Hongmei Jiang,Yuanhua Shao,Xiaoli Qin
A simple and rapid electrochemical synthesis strategy for in-situ fabrication of toluidine blue (TB)-functionalized metal organic framework (TB@MOFs) thin films via one-step reduction approach was reported. The TB@MOFs thin layer has been synthesized on the working electrode surface in different times at room temperature , and its morphology and thickness was characterized by varied instruments, including scanning electron microscope, transmission electron microscope, and scanning ion conductance microscope. The prepared TB@MOFs shows exceptional electrochemical activity and stability under mild conditions, and its electrochemical response is dependent on the amount of TB molecules which are encapsulated in the MOFs. Based on the TB@MOFs sensing platform, a sensitive label-free electrochemical immunosensor for the detection of Indole-3-acetic acid (IAA, a plant hormone) was developed with a wide linear range (2.5 pg mL −1 - 5 ng mL −1 ) and a low detection limit (1.4 pg mL −1 ). Moreover, this immune sensor shows good sensitivity and selectivity in real samples for IAA detection.
研究领域:
分析化学
·
植物学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）