CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Yifang Cao,Ruhong Qiu,Wang Yutao,Jintao Zhou,Jun Xiong,Niya Ta,Xiaoling Wu,Wenyong Lou
Biomimetic zinc single-atom nanozymes mimicking natural lactonase were designed. • The nanozyme integrates lactonase, peroxidase, and oxidase-like activities. • Zn-Nx coordination and pore structure govern the multi-enzyme performance. • ZIF-8-Zn-SAC was immobilized onto silicone catheters via an in-situ hydrogel. • The surface exhibits synergistic quorum-quenching and antibiofilm performance. Bacterial biofilms on indwelling medical devices pose a critical challenge due to their intrinsic resistance to antibiotics and host immune defenses. Herein, a zeolitic imidazolate framework (ZIF)-derived zinc single-atom nanozyme (ZIF-8-Zn-SAC) was developed as a multifunctional catalytic material for antibacterial and antibiofilm applications. The atomically dispersed Zn-N x active sites in the N-doped carbon framework provide a biomimetic coordination environment resembling the catalytic center of natural lactonase. ZIF-8-Zn-SAC exhibited integrated lactonase-, peroxidase (POD)-, and oxidase (OXD)-like activities. Its lactonase-like activity enabled efficient degradation of N-acyl homoserine lactone signal molecules, thereby disrupting quorum sensing and suppressing Pseudomonas aeruginosa biofilm formation. Meanwhile, its POD- and OXD-like activities catalyzed the generation of reactive oxygen species from H 2 O 2 and dissolved O 2 , leading to oxidative bacterial damage. Structure-activity relationship analysis indicated that Zn-N x coordination, nitrogen species, and pore accessibility were associated with the multi-enzymatic catalytic performance. As a proof-of-concept surface engineering demonstration, ZIF-8-Zn-SAC was immobilized onto silicone catheter surfaces through an in-situ hydrogel coating strategy. The resulting nanozyme-functionalized surfaces showed effective inhibition of P. aeruginosa biofilm formation, favorable preliminary in vitro biocompatibility, and retained mechanical stability. This work provides a structure-activity-guided strategy for designing multifunctional nanozymes and highlights their potential for antibiofilm surface engineering on polymeric medical interfaces. Schematic illustration of the design and antibiofilm mechanism of ZIF-8-Zn-SAC nanozymes. ZIFs-derived Zn-N x active sites embedded in an N-doped carbon framework provide biomimetic lactonase-like activity for AHLs degradation and quorum-sensing disruption. Simultaneously, the nanozyme exhibits POD- and OXD-like activities, catalyzing ROS generation from H 2 O 2 and dissolved O 2 to induce oxidative bacterial damage. As a proof-of-concept surface engineering strategy, ZIF-8-Zn-SAC was immobilized onto silicone catheter surfaces via an in-situ hydrogel coating, enabling effective inhibition of Pseudomonas aeruginosa biofilm formation on device-relevant polymeric interfaces. Download: Download high-res image (318KB) Download: Download full-size image
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Journal of Advanced Research
·
2026
·
IF 17.1
Sumei Ling,Yang Xu,ZhouJie Xu,Xiaoli Li,Linfang Lu,Menghan Sun,Wenjun Yang,Yuxuan Liu,Jun Yuan,Shihua Wang
Introduction Sulfamethazine (SM2) antibiotic residues in food pose a significant risk to human health, including pathogenic bacterial resistance, allergic reactions, and carcinogenicity. Current lateral flow immunoassay (LFIA) typically employing colloidal gold (AuNP) as a signal reporter exhibits unsatisfactory sensitivity. Recently, metal–organic frameworks (MOFs) nanomaterial as a supporting matrix for loading nanoparticles provides a powerful tool to improve the performance of LFIA. Objectives This study aims to develop an enhanced immunosensor based on antibody labeled Au/Ir@Cu/Zn-MOF signal amplification nano-composite for sensitive and point-of-care monitoring of SM2 antibiotic residues. Methods Firstly, the highly specific monoclonal antibody (mAb) against SM2 belonging to the IgG1 subclass was screened. Subsequently, the Au/Ir@Cu/Zn-MOF nanocomposite was synthesized by incorporating Au/Ir bimetallic nanoparticles into the previously prepared Cu/Zn-MOF composite. The anti-SM2 mAb was then conjugated to Au/Ir@Cu/Zn-MOF nanocomposite via a co-precipitation method to fabricate the mAb-Au/Ir@Cu/Zn-MOF probe. Finally, a lateral flow immunoassay was constructed for the detection of SM2 residues. Results The vLOD the Au/Ir@Cu/Zn-MOF-based LFIA for SM2 detection was approximately 2.5 ng/mL, which was at least 600 times greater than that of the AuNP-based LFIA (with the vLOD of 1563 ng/mL). Average recovery rates were 80.08–90.8% in milk sample, 88–89.3% in eel sample and 85.3–95.2% in pork sample, respectively. Conclusion The Au/Ir@Cu/Zn-MOF nanocomposite utilized as signal amplification probe demonstrated remarkable sensitivity for the detection of SM2. The developed Au/Ir@Cu/Zn-MOF-based LFIA exhibits significant potential for the rapid and sensitive detection of contaminants in environmental monitoring and food safety.
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ADVANCED FUNCTIONAL MATERIALS
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2026
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IF 19
Wenjia Liu,Nina Zhang,Xu Guo,Xiaofan Zhang,Ying Wang,Jinchao Zhang,Kun Ge,Guoqiang Zhou
Immunotherapy is rapidly emerging as a potent, targeted therapy for breast cancer. However, the low immunogenicity and immunosuppressive tumor microenvironment (TME) in breast cancer limit its efficacy. In this study, a novel nanoreactor, CeO 2 :Mn (CM) embedded with the zeolitic imidazolate framework-90 (ZIF-90) and loaded with 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), is developed and designated CeO 2 :Mn/ZIF-90/ABTS (CMZA). Zn 2+ release from ZIF-90 degradation in the TME triggers pyroptosis and elevates intracellular •O 2 − levels by inhibiting the electron-transport chain. Furthermore, the CM nanozyme exhibits multienzyme activities, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), which convert •O 2 − to H 2 O 2 , generate •OH, produce O 2 , and oxidize ABTS to oxidized ABTS (oxABTS). The oxABTS exhibits strong near-infrared absorption and converts 808 nm light into heat, enhancing CM catalysis and depleting intracellular glutathione (GSH), thereby further amplifying the accumulation of reactive oxygen species (ROS). Collectively, CMZA induces NLRP3/c-Caspase-1/GSDMD-N pyroptosis through Zn 2+ release and photothermal-enhanced multienzyme activity, reshaping the TME and boosting immunogenicity to limit tumor progression and metastasis.
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JOURNAL OF MOLECULAR STRUCTURE
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2026
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IF 4.9
Zhaoying Su,Lu Ren,Qian Sun,Han Ye,Ping Wang,Meixuan Chen,Dawei Zhang
A novel imidazole-based hydrazide fluorescent probe, ( E )- N' -((1 H -imidazol-2-yl)methylene)- 4-(4,5-diphenyl-1 H -imidazol-2-yl)benzohydrazide (HDB), was designed and synthesized. The chemical structure of HDB was fully characterized by FT-IR, 1 H NMR, 13 C NMR, and HR-MS, confirming the successful synthesis of the target product. Subsequently, the sensing performance of HDB was systematically investigated via fluorescence spectroscopy and ultraviolet-visible absorption spectroscopy. The probe HDB exhibited naked-eye distinguishable recognition of Cu 2+ with high sensitivity and selectivity in the EtOH/H 2 O (1:1, v/v), where fluorescence quenching was triggered by the chelation between HDB and Cu 2+ . Performance evaluation results demonstrated that the detection limit of HDB for Cu 2+ was as low as 0.38 nM. Job’s plot analysis revealed a 1:1 binding stoichiometry between HDB and Cu 2+ , with a binding constant of 1.765×10 5 M −1 . Cyclic experiments involving the alternate addition of Cu 2+ and Na 2 EDTA verified the excellent reusability of HDB. The practical applicability of HDB was validated through quantitative detection of Cu 2+ in real water samples, where the obtained recoveries fell within an acceptable range, indicating its feasibility for trace Cu 2+ analysis in environmental water matrices. Furthermore, this study explored the potential application of HDB in fingerprint detection, providing a new avenue for the innovation of fingerprint identification and anti-counterfeiting technologies. Notably, HDB achieved visualized monitoring of Cu 2+ in living cells, which further expands its application prospects in the biomedical field.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2026
·Vol. 65
·期 34
·18449-18463
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IF 3.9
Kasuri Amna,Khan Palwasha,Khan Asim Laeeq,Gilani Mazhar Amjad,AlMohamadi Hamad,Batool Mehwish,Usman Muhammad,Zaman Muhammad,Yasin Muhammad
Mixed matrix membranes (MMMs) are promising for overcoming
the
permeability–selectivity limitations of polymeric membranes,
yet their performance is often restricted by filler aggregation and
interfacial defects at high loadings. Here, we introduce ZIF-90 in
a porous liquid-like dispersion form as a filler for high-performance
MMMs. Carbene functionalization provides liquid-like interfacial compatibility
while preserving the microporosity of ZIF-90, enabling homogeneous
nanoscale dispersion within a polydimethylsiloxane (PDMS) matrix at
high loading. This architecture creates continuous selective transport
pathways and suppresses nonselective interfacial defects. Consequently,
the ZIF-90-PL/PDMS membrane exhibits enhanced permeability and selectivity,
achieving a total flux of 3.86 kg m –2 h –1 and a separation factor of 29.3 for ethanol recovery at 60 ° C
with 45 wt % loading, outperforming conventional MMMs. Mechanistic
analysis reveals that functionalization enhances ethanol-selective
sorption and reduces diffusion resistance, mitigating the permeability–selectivity
trade-off. These findings establish the porous liquid-like MOF dispersion
as an emerging class of membrane fillers for future separation membranes.
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MICROCHEMICAL JOURNAL
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2026
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IF 5.2
Lingsong Lu,Bei Liu
3D porous Fe 3 O 4 @ZIF-90 interface provides high-density DNA loading sites. • Mn 2+ -gated DNAzyme walker ensures a low-background controllable assay. • DNAzyme walker and CRISPR-Cas13a cascade enable dual signal amplification. • The biosensor demonstrated high accuracy and specificity in human serum samples. The precise quantification of Mucin-1 (MUC1) is vital for the early diagnosis and therapeutic monitoring of epithelial malignancies. In this study, a fluorescent biosensing platform based on a 3D porous metal-organic framework (MOF) and a cascaded signal amplification strategy was developed for MUC1 detection, driven by a target-induced conformational switch and Mn 2+ -dependent catalytic DNAzyme walking. Porous Fe 3 O 4 @ZIF-90 nanocomposites were used to construct the sensing interface, which co-immobilized dormant DNAzyme/aptamer duplexes and a high-density layer of substrate DNA (sub-DNA), serving as a robust platform for the subsequent walking process. Upon specific MUC1 recognition, the aptamer preferentially captures the target MUC1. The thermodynamic driving force induces the dissociation of the MUC1/aptamer complex, liberating the DNAzyme engine. Following magnetic separation to eliminate matrix interferences, the addition of Mn 2+ triggers the processive inter-molecular migration of the DNAzyme walker across the sub-DNA immobilized 3D porous Fe 3 O 4 @ZIF-90 interface via successive hybridization and site-specific cleavage of internal riboadenosine (rA) junctions. This inter-molecular walking process generates a high flux of trigger strands, which subsequently activates the CRISPR-Cas13a system for a robust trans -cleavage of dual-labeled fluorophore-quencher (FQ) reporters. The resulting degradation of FQ reporter enables sensitive MUC1 quantification through exponential fluorescence recovery. By synergizing the structure advantages of the 3D MOF with this cascaded signal amplification, the biosensor achieved a wide linear range from 0.37 nM to 500 nM with a low detection limit of 17.2 pM. Furthermore, the biosensor exhibited reliable specificity and recovery (102.28%–106.94%) in complex human serum assays. The proposed strategy effectively minimizes non-specific background leakage, demonstrating promising potential for the ultra-sensitive detection of MUC1 in complex biological matrices. Schematic presentation of the fluorescent biosensor for the determination of Mucin-1 based on a 3D porous Fe 3 O 4 @ZIF-90 interface and DNAzyme walker-mediated CRISPR-Cas13a cascade. Download: Download high-res image (117KB) Download: Download full-size image
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INTERNATIONAL JOURNAL OF PHARMACEUTICS
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2026
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IF 6
Pingfei Li,Yongqi Liu,Jie Zhang,Tianyi Wang,Shiming Zhang,Xinggang Yang
Owing to the complexity of the tumor microenvironment, monotherapy often suffers from inherent limitations and fails to achieve satisfactory antitumor efficacy. Therefore, multimodal collaborative therapy has become a trend. In this study, CP@ZIF-PQ/Ce6 NPs, a ZIF-90-based multimodal nanosystem loaded with copper peroxide (CP) nanoparticles, the autophagy inhibitor primaquine phosphate (PQ) and the photosensitizer Ce6, were developed. This nanosystem enables the integration of photodynamic therapy (PDT), chemodynamic therapy (CDT), oxygen generation, and autophagy inhibition. Through the combined therapeutic effects of PDT/CDT/O 2 generation/autophagy inhibition, CP@ZIF-PQ/Ce6 NPs enhance the production of reactive oxygen species(ROS), alleviate tumor hypoxia, and block the autophagy-mediated protective pathway in tumor cells. Moreover, the nanosystem exhibits dual pH- and ATP-responsive properties, allowing tumor microenvironment-triggered drug release. CP@ZIF-PQ/Ce6 NPs successfully integrate PDT, CDT, oxygen-generating therapy, and autophagy inhibition, demonstrating potent antitumor activity and favorable biocompatibility both in vitro and in vivo. This study provides an innovative strategy and experimental evidence for multimodal collaborative antitumor therapy to overcome the limitations of monotherapy imposed by the tumor microenvironment. Download: Download high-res image (147KB) Download: Download full-size image
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JOURNAL OF NANOBIOTECHNOLOGY
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2026
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IF 15
Wenjia Liu,Ying Wang,Xiangyu Hao,Yangxun Wang,Xiaofan Zhang,Jinchao Zhang,Kun Ge,Guoqiang Zhou
Triple-negative breast cancer (TNBC) is associated with rapid progression and poor sensitivity to monotherapies, necessitating combination strategies to enhance tumor immunogenicity, reprogram the tumor microenvironment (TME), and convert immunologically "cold" tumors into "hot" ones. In this study, we develop a pH-responsive, pyroptosis-inducing nanoreactor, ZnS/ZIF-90:Fe/Vk3 (ZZFV), designed to elicit potent antitumor immunity. Under acidic conditions, ZZFV rapidly degrades, releasing Zn 2+ , Fe 2+ , H 2 S, and Vk3. H 2 S promotes glucose uptake and consumption while downregulating cytochrome c oxidase subunit IV (COX IV) expression, whereas Zn 2+ suppresses lactate dehydrogenase (LDH) activity, collectively disrupting glucose metabolism and exacerbating intracellular acidification. Meanwhile, NAD(P)H quinone oxidoreductase 1 (NQO1), highly expressed in tumor cells, catalyzes the conversion of Vk3 to H 2 O 2 , which is subsequently converted to highly toxic •OH through Fe 2+ -mediated Fenton reactions. Additionally, Zn 2+ and H 2 S synergistically disrupt mitochondrial function, thereby amplifying reactive oxygen species (ROS) production. Excessive ROS triggers Caspase-1-dependent pyroptosis, leading to the liberation of damage-associated molecular patterns. These events promote dendritic cell (DC) maturation, robust T-cell activation, M1 macrophage polarization, and expansion of central memory T-cell populations, thereby conferring durable immune protection. Consequently, ZZFV demonstrates potent antitumor efficacy, effectively overcoming immune tolerance in TNBC and achieving robust, sustained antitumor immunity.
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Small Methods
·
2025
·
IF 9.1
Hang Ao,Wencheng Xiao,Weiwei Chen,Wenrui Hu,Jie Wu,Huangxian Ju
Chemiluminescent immunoassay is the most widely used protein detection technique in clinical diagnosis but still faces substantial challenges in antibody-related single-cell analysis due to the lack of a homogeneous immunoassay with strong and stable chemiluminescence signal. Herein, a single-cell microfluidic platform for efficient in situ detection of antibody secreted from single hybridoma cells through a microcavity chemiluminescent immunoimaging (MCCLII) protocol is reported, which consist of an imidazole-enhanced chemiluminescence system, a proximity-triggered DNA nanomachine and a hybrid-regulated hemin-DNA switch. In MCCLII, antibody secreted from single hybridoma cells can be visualized through a homogeneous proximity CL assay, which converses the target antibody to DNA for triggering the DNA nanomachine and then activates the catalytic activity of hemin-DNA switch to produce strong and persistent chemiluminescence for micro-imaging. The MCCLII realizes immunoimaging detection down to 66 antibody molecules in 0.79-nL microchamber and demonstrates the possibility of specific hybridoma cell screening within 30 min, which provides a simple and fast antibody screening platform to promote antibody-drug production.
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Journal of Materials Chemistry A
·
2025
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IF 9.5
Jianfeng Hu,Huiya Cheng
A challenge in metal protection is developing anti-corrosion materials that possess both high adsorption capacity and intelligent responsiveness. To overcome the limitations of traditional corrosion inhibitors, a novel pH-responsive corrosion inhibitor (PDT@ZIF-90) was developed through a multi-step strategy. Specifically, the catechol-modified polymer–TiO2 hybrid (PDT) was synthesized through Schiff base reactions, which demonstrated dense adsorption on Q235 carbon steel through Fe–N/O coordination. Subsequent ZIF-90-mediated in situ encapsulation enabled precise payload integration of the PDT corrosion inhibitor. UV-vis characterization confirmed that the PDT@ZIF-90 composite system enables pH-triggered intelligent release of the active corrosion inhibitor component under acidic conditions (pH < 6). Electrochemical tests revealed a 95.2% inhibition efficiency for 200 ppm PDT@ZIF-90 in 3.5 wt% NaCl. This dual-functional strategy offers a scalable paradigm for self-adaptive anticorrosion materials suitable for Q235 carbon steel in marine and energy infrastructure applications.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Xiangyu Zi,Xiaolong Sun,Fengwei Yin,Jia Gao,Jie Huang,Yingying Zhang,Yao Chen,Yongqian Fu
MOFs represent a promising platform for enzyme immobilization, yet the development of tailored strategies to accommodate both enzyme properties and MOF structures remains a challenge. In this study, we employed chitosanase BCC1 as a model enzyme and integrated Fe 2+ /Mn 2+ ions into the ZIF-8 to construct a bimetallic MOF-based immobilization system (BCC1@Fe/Mn-ZIF-8). Comprehensive characterization by SEM, FTIR, TGA confirmed the successful immobilization and structural integrity of the composite. The immobilized enzyme retained 97 % of its initial activity and exhibited enhanced stability under harsh thermal, pH, and solvent conditions. Notably, BCC1@Fe/Mn-ZIF-8 maintained 117 % activity after 14 days at 4 °C and 72 % residual activity after 14 reuse cycles, indicating excellent storage and reusability. This bimetallic MOF-based strategy offers a robust and efficient approach for enzyme immobilization, with strong potential in industrial biocatalysis.
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ACS Applied Bio Materials
·
2023
·
IF 4.7
Zhong-Qin Pan,Sha-Li Yu,Zeng-Qiang Wu,Kang Wang
Artificial oxygen carriers, such as favorably hemoglobin-based oxygen carriers, have received considerable attention due to some drawbacks of human donor blood. Among all oxygen carriers, the metal organic framework (MOF) exhibits excellent oxygen-carrying capacity due to its good encapsulation efficiency and competitive biocompatibility. Recently, zeolitic imidazolate frameworks (ZIFs) with unique structure have attracted much attention due to their outstanding solvothermal stability. Notably, ZIF-8, the prototypical ZIF, has been utilized to load hemoglobin (Hb) as a potential blood substitute. In this work, another ZIF material, which possesses a high oxygen binding/release capability, suitable safety profile, high stability, and efficiency as a potential oxygen carrier, was used to encapsulate Hb in an environment-friendly condition.
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