BIOCHEMICAL PHARMACOLOGY
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2026
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IF 6.5
Han Zhang,Ling-Li Lu,Hua-Jie Wang,Pei Wang,Jian-Guo Chen,Fang Wang,Yang Liu,Hong-Sheng Chen,Peng-Fei Wu
Numerous commonly used antibacterial agents frequently induce acute anorexia as a clinical side effect, yet the underlying mechanism remains poorly understood. Here, we investigated the role of gut bacteria-derived hydrogen sulfide (H 2 S) signaling in the regulation of feeding behavior and its involvement in antibiotic-associated anorexia. Using methylene blue colorimetric and fluorescent probe-based detection, we found that 24-h fasting elevated H 2 S levels in rat feces and serum, which robustly stimulated feeding behavior. 16S rRNA sequencing revealed that fasting reshaped the gut microbiota and enriched certain H 2 S-producing bacterial taxa. Through fecal microbiota transplantation and pharmacological manipulations, our findings suggest a contribution of microbiota-derived H 2 S to fasting-induced feeding behavior. Mechanistically, fasting-induced H 2 S promoted feeding by activating AMP-activated protein kinase (AMPK) in the hypothalamic arcuate nucleus, a key center for feeding regulation, via an S-sulfhydration-dependent mechanism, which in turn enhanced the activity of neuropeptide Y-positive neurons. Furthermore, we showed that impaired gut bacterial H 2 S signaling contributed to metronidazole-induced acute anorexia, a common adverse clinical effect. Given that H 2 S-producing taxa, especially Desulfovibrio , are sensitive to a broad range of antibacterial agents, our findings suggest an important role for gut bacterial H 2 S signaling in gut-brain communication and appetite control, and point to a microbiota-host feedback mechanism underlying antibacterial-induced acute anorexia.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Yaowei Zhu,Ruijie Tang,Jun Zhao,Changlong Jiang,Lingfei Li,Bianhua Liu
Tetracyclines antibiotics (TCs) are widely used as broad-spectrum antibiotics, but their overuse leads to environmental pollution and threatens human health. Existing detection techniques (mass spectrometry, etc.) are complicated and cumbersome, and do not allow for rapid detection. Consequently, it is imperative to develop a sensing technology for rapid on-site detection of full-spectrum TCs residues. The UV–visible absorption peaks of TCs overlap with the blue fluorescence excitation wavelength range, which can “turn-off” the blue fluorescence based on the internal filtering effect. Additionally, the β-diketone bond of TCs can bind to Eu 3+ , sensitizing Eu 3+ to produce red fluorescence. Thus, we constructed a selective sensing chemo-fluorescent sensor using blue nitrogen-doped carbon dots (BCDs) and europium metal–organic framework (Eu-MOF). As the concentration of TCs increased from 0 to 2.6 μM, the sensor exhibited a continuous change in fluorescence color from blue to purple to red. The limit of detection is 1.37 nM with a response time of less than 1 s. Furthermore, this study presents a portable capillary chemo-fluorescent sensor detection kit, which consists of a capillary chemo-fluorescent sensor comprising BCDs/Eu-MOF encapsulated in a self-designed non-fluorescent glass capillary, as well as a color recognizer with a kit produced using 3D printing technology. This fluorescence sensing technology with excellent sensitivity and selectivity provides technical support for the rapid detection of TCs and the reduction of pollutant hazards to sustainable human development.
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JOURNAL OF MEMBRANE SCIENCE
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2024
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IF 8.4
Qing-Pu Zhang,Hua-Wen Peng,Hui Ma,Yu-Ling Sun,Qiang Zhao,Chuan-Feng Chen,Chun Zhang
For antibiotics, the purification and separation in the synthesis process, as well as enrichment and recycling in the utilization process, has a profound impact on human health and environmental issues, which pose an unignorable challenge for selective molecular sieving of separation technology. Herein, we offered a convenient high-accuracy separation membrane construction by using three dimensional rigid triptycenes as building-blocks to fabricate porous triptycene networks polyarylate nanofilms (PTN-NFs) by interfacial polymerization. With permanent porous structure and excellent stability, the PTN-NF membrane exhibited remarkable separation precision around 90 g mol −1 scale for several mixture separation and significant selectivity as high as 39.3 for piperacillin/6-aminopenicillanic acid (6-APA) separation.
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ANALYTICA CHIMICA ACTA
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2025
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IF 6
Ximei Zhang,Lanzhen He,Guangmin Zhang,Fenying Kong,Lanlan Chen
Background Escherichia coli ( E. coli ) is a model microorganism extensively used in microbial research due to its well-characterized physiology and genetic tractability. Monitoring its physiological indicators, such as metabolic activity and stress responses, is essential for understanding microbial life processes. However, existing methods often lack sensitivity, rapidity, and biocompatibility. This study addresses these limitations by combining electrochemiluminescence (ECL) biosensing with a biocompatible interface, to enable precise and efficient monitoring of E. coli 's physiological state. Results This study presents a novel ECL biosensor that leverages the biocompatibility of bovine serum albumin (BSA) with E. coli . The biosensor employs horseradish peroxidase (HRP) and 4-chloro-1-naphthol (4-CN) to induce a precipitation reaction that quenches the ECL signal of CdS–K 2 S 2 O 4 , enabling the sensitive detection of endogenous hydrogen peroxide (H 2 O 2 ) produced by E. coli . The sensor exhibits excellent linearity, stability, and reproducibility, with a detection limit of 54 nM for H 2 O 2 . Additionally, the biosensor effectively distinguishes between antibiotic-resistant and sensitive strains of E. coli , demonstrating its potential for comprehensive physiological assessment. Significance This innovative ECL biosensor represents a significant advancement in microbial monitoring, combining high sensitivity, rapidity, and biocompatibility, offering a powerful tool for comprehensive physiological monitoring in bioanalytical sciences. Its potential applications in biotechnology, food industry, and drug research highlight its practical relevance.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2025
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IF 2.8
Cuiyao He,Saisai Ling,Ying Jin,Lisha Fu,Xiaoke Dai,Xiaohui Qi,Yiying Wu,Zhenglei Wang,Yuhua Deng
Piperacillin/tazobactam and metronidazole are two commonly antimicrobials used to prevent and treat infections after liver transplantation in pediatric patients. However, under the recommended dose, it may lead to insufficient antimicrobial treatment and poor efficacy. To achieve antimicrobial therapy based on pharmacokinetic strategies, a rapid and highly sensitive ultra-high performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to measure the plasma concentrations of piperacillin, tazobactam, and metronidazole in pediatric liver transplant recipients. Sample separation was achieved using an Acquity UPLC CSH C 18 column (2.1 × 50 mm, 1.7 μm) with gradient elution. The mobile phase consisted of ammonia solution–formic acid–water (0.5:1:1000, v / v /v) and methanol–acetonitrile (1:1, v/v). The plasma concentration range was 0.20–100.00 μg/mL, with good linear range. The intra- and inter-day precisions were less than 13.4 %, and the accuracy ranged from 90.0 % to 109.0 %. The selectivity, carryover, dilution integrity, matrix effect, recovery, and stability met the requirements of the relevant guidelines. The established UPLC–MS/MS method was successfully applied to measure the concentrations of piperacillin, tazobactam, and metronidazole in the plasma of pediatric liver transplant patients. The results showed that according to the recommended dosing regimen, piperacillin/tazobactam reached the effective therapeutic target of 50 %T > MIC in only 4 h. The maximum plasma concentration (C max ) of metronidazole ranged from 9.46 to 28 μg/mL, with an average C max of 16.59 μg/mL, which did not achieve the ideal C max /MIC ratio of 8–10.
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Frontiers in Cellular and Infection Microbiology
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2025
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IF 4.8
Jiansheng Lin,Chunyan Lin,Jingyang Zheng
Background: P. diazotrophicus was isolated from a newborn with D-galactosemia complicated sepsis. A homologous blaCAR-2 gene, encoding CAR-2, a predicted member of the CAR family subclass B3 metallo-β-lactamases (MBLs), was found in the genome of this strain. This study aimed to explore the identification of a novel CAR-2 protein encoded by the chromosome of P. diazotrophicus Pd1 that exhibits the zinc-binding motifs of subclass B3 enzymes and the regulatory pattern of CARR, located directly upstream of the blaCAR-2 gene, on the blaCAR-2 gene and its impact on antibiotic resistance.Methods: Antibiotic susceptibility testing was conducted by the plate agar dilution method. Site-directed mutagenesis was conducted using Mut Express II Fast Mutagenesis Kit V2. Kinetic assays were used to determine the hydrolysis of β-Lactam. The construction of bacterial knockout strains was carried out according to the principle of homologous recombination. The detection of the mRNA expression level of the gene was performed by Real-time Quantitative PCR (qPCR).Results: The minimum inhibitory concentrations (MICs) for E.coli DH5α(pHSG398::CAR-2), which expressed blaCAR-2, increased significantly for cefalothin, cefuroxime and cefotaxime sodium by 2-, 16- and 32- fold, respectively, which showed that blaCAR-2 had resistance to these three antibiotics. This protein, which was a MBL, contains two classical zinc-binding sites characteristic of subclass B3, with the amino acid motif His136, His138, His211, Asp140, His141, and His276. Among the six residues, His136, Asp140, and His211 exhibited the highest catalytic activity. We determined that CAR-2 can effectively hydrolyze cefalothin, cefuroxime and cefotaxime. The chloramphenicol resistance of the constructed E. coli DH5α strain was significantly reduced in the presence of CARR than in the absence of CARR. Compared with those for the wild-type P. diazotrophicus, MICs of cefalothin, cefuroxime and cefotaxime for the ΔCARR strain increased by 8-, 8- and 16-fold, respectively, and the expression level of blaCAR-2 also increased by approximately 10-fold.Conclusion: Overall, CAR-2 is a novel subclass B3 MBL of the CAR family that exhibits catalytic activity against cefalothin, cefuroxime and cefotaxime. CARR represses the expression of blaCAR-2, thereby reducing the resistance of P. diazotrophicus to these antibiotics. This provided a theoretical basis for revealing new mechanisms of pathogen resistance.
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