Microorganisms
·
2022
·Vol. 10
·期 3
·565
·
IF 4.926
Hao Tan,Delong Kong,Qingyun Ma,Qingqing Li,Yiqing Zhou,Xu Jiang,Zhiye Wang,Rebecca E. Parales,Zhiyong Ruan
In this study, theCutaneotrichosporon dermatisstrain M503 was isolated and could efficiently degrade tetracycline, doxycycline, and chlorotetracyline. The characteristics of tetracycline degradation were investigated under a broad range of cultural conditions. Response surface methodology (RSM) predicted that the highest degradation rate of tetracycline could be obtained under the following conditions: 39.69 °C, pH of 8.79, and inoculum dose of 4.0% (v/v, ~3.5 × 106cells/mL in the medium). In accordance with the five identified degradation products of tetracycline, two putative degradation pathways, which included the shedding of methyl and amino groups, were proposed. Moreover, the well diffusion method showed that the strain of M503 decreases the antibacterial potency of tetracycline, doxycycline, and chlorotetracycline. These findings proposed a putative mechanism of tetracycline degradation by a fungus strain and contributed to the estimation of the fate of tetracycline in the aquatic environment.Keywords:tetracycline;biodegradation;Cutaneotrichosporon dermatis;response surface methodology (RSM);degradation products;antibacterial potency
研究领域:
环境科学
·
微生物学
·
药理学
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DOI
PubMed
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2023
·
IF 5.1
Siyuan Yu,Yanting Zhou,Chunyue Zhao,Han Liu,Zhiyun Xue,Jinfeng Xing
Dispersion photopolymerization was carried out under green LED irradiation to rapidly prepare monodisperse poly(methyl methacrylate) (PMMA) microspheres with small size by using a high-efficient three-component initiating system. The polymerization parameters including exposure time, MMA content, stirring speed, water ratio and PVP content were systematically investigated. Crucial parameters were further optimized by response surface method (RSM), and a corresponding polymerization mechanism was proposed. Finally, PMMA microspheres with 375.6 nm of size and 0.101 of PDI were prepared. This study provides a facile method for the rapid fabrication of PMMA microspheres.
研究领域:
材料科学
·
高分子化学
·
纳米技术
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DOI
高影响因子
Foods
·
2023
·Vol. 12
·期 18
·3438
·
IF 5.2
Chaoqun Sun,Guodong Wang,Jing Sun,Jiyong Yin,Jian Huang,Zizi Li,Di Mu,Menglu He,Tingting Liu,Jiali Cheng,Hanchen Du,Yan Chen,Wenjie Qu
Polygonatum sibiricumPolysaccharide (PsP) with antioxidant function is the main active component ofPolygonatum sibiricum(P.sibiricum). The currently poor extraction yield and extraction methods of PsP cannot meet the application of that in food industrial production. In this research, an ultrasound-assisted extraction-deep eutectic solvents (UAE-DESs) method, which has never been used in the PsP industry, was first used to extract PsP. The extraction conditions were optimized by the response surface method (RSM). Both the extraction yield and antioxidant function were simultaneously considered during the optimization process. The indicators of PsP’s level and antioxidant activity in vitro were used to present the extraction yield of the UAE-DESs method, the purity, and the antioxidant effect of PsP. Under the optimal conditions, which included a liquid–solid ratio of 26:1 (mL:g), extraction temperature of 80 °C, ultrasonic time of 51 min, and ultrasonic power of 82 W, the PsP extraction yield could reach (43.61 ± 0.09)%, which was obviously higher than single DESs (33.81%) and UAE (5.83%), respectively, and the PsP appeared favorably antioxidant function. This research proposed an efficient extraction method for PsP, filled the basic research gap, and further improved the development of PsP as a dietary supplement with antioxidant function in the food industry.Keywords:Polygonatum sibiricumPolysaccharide (PsP);ultrasound-assisted extraction-deep eutectic solvents (UAE-DESS);antioxidant function;response surface method (RSM)
研究领域:
食品科学
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药学
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生物技术
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DOI
PubMed
高影响因子
ACS Omega
·
2024
·
IF 3.7
Rui Wang,Chaolong Rao,Qiuyan Liu,Xiong Liu
Zanthoxylum alkylamides, as a numbing substance in Zanthoxylum bungeanum has many physiological effects. However, the numbing taste and unstable properties limited its application. This study aimed to optimize the preparation process of Zanthoxylum alkylamides liposomes by response surface methodology (RSM) and to investigate the in vitro absorption characteristics of the liposomes through the Caco-2 cell monolayer model. The process parameters of liposomes were as follows: Zanthoxylum alkylamides was 15 mg, phospholipid–feedstock ratio was 6.14, phospholipid–cholesterol ratio was 8.51, sodium cholate was 33.80 mg, isopropyl myristate was 29.49 mg, and the theoretical encapsulation efficiency of the prepared liposomes could reach 90.23%. Further, the particle size of the liposomes was 155.47 ± 3.16 nm, and the ζ-potential was −34.11 ± 4.34 mV. Meanwhile, the liposomes could be preserved for 14 days under the condition that the content of Zanthoxylum alkylamides was less than 2 mg/mL and the preservation temperature was lower than 25 °C. Moreover, the uptake characteristics of the Zanthoxylum alkylamides liposomes in the Caco-2 cell monolayer model were also investigated. The results showed that the Zanthoxylum alkylamides liposomes could be taken up and absorbed by Caco-2 cells. Also, the Zanthoxylum alkylamides liposomes had a better uptake performance than the unembedded Zanthoxylum alkylamides and conformed to the passive uptake.
研究领域:
药学
·
药物递送系统
·
生物药剂学
·
药代动力学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2018
·
IF 3.909
Yujuan Li,Yizhou Xin,Fangxue Xu,Mengmeng Zheng,Xiaozhi Xi,Xiaowei Cui,Hui Cao,Hong Guo,Chunchao Han
The maca polysaccharides optimal extraction conditions were obtained by using response surface methodology (RSM) method and the anti-fatigue activity of maca polysaccharides (MCP) was explored. The maca polysaccharides extract yield of RSM could reach 9.97 mg/g by using the model predicts, and the total sugar and protein purity were 61.00% and 4.46% with the further isolation process, respectively. And the monosaccharide compositions obtained by gas chromatograph (GC) were composed of rhamnose (rha), glucose (glc), galactose (gal) with the ratio of 2.34:10.21:1.00. Furthermore, the anti-fatigue activity was evaluated by the swimming parameter, biochemistry parameters (liver glycogen (LG), blood urea nitrogen (BUN), and lactic acid (LD)), the result indicated that the low-dose maca polysaccharides group had the significant anti-fatigue activity.
研究领域:
药学
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食品科学
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生物化学
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DOI
PubMed
高影响因子
APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING
·
2022
·Vol. 128
·期 12
·1-18
·
IF 2.983
He Yuan,Guo Shenghui,Li Shiwei,Yin Shaohua
Y Zeolite plays an irreplaceable role in many important applications due to its unique physicochemical properties, which has attracted extensive attention from researchers. Response surface methodology (RSM) was used to optimize the process parameters for the hydrothermal synthesis of Y zeolite using kaolin activated by microwave heating. The best relative crystallinity was 108.17% could be achieved at the condition: the aging temperature of guiding agent was 65 ℃, the crystallization temperature was 100 ℃, the crystallization time was 24 h. In addition, the aging temperature of guiding agent was the most important influence factor. Under the optimal condition, the SiO 2 /Al 2 O 3 ratio of synthesized Y zeolite was 4.54, 90% of the particle size was less than 60 μm, and the specific surface area of Y zeolite was 479 m 2 /g. The successful preparation of Y zeolite with high crystallinity is expected to provide reference for the application of wastewater treatment, CO 2 sequestration and fluid catalytic cracking for petroleum.
研究领域:
材料科学
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化学工程
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环境科学
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DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2021
·
IF 4.291
Ao Li,Linbei Xie,Sijie Zhou,Min Zhang,Yi Ding,Ping Wang
In this study, the magnetic core-shell nanomaterial Fe 3 O 4 /SiO 2 /N-TiO 2 /Ag/AgCl (FSNT/Ag/AgCl) that efficiently degraded methyl orange (MO) was produced. The dosages of tetrabutyl titanate (TBOT), urea and AgNO 3 were optimized by response surface methodology (RSM). Scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray photoelectron spectroscopy analysis (XPS), energy dispersive spectrometer (EDS), X-ray diffractometer (XRD), fourier transform infrared spectrometer (FT-IR), and UV–vis diffuse reflectance spectrum (UV–vis DRS) were used to characterize the nanomaterial. The particle size of FSNT/Ag/AgCl was 91.98 nm, and it presented a core-shell structure with Fe 3 O 4 as the core, SiO 2 and N-TiO 2 wrapped in Fe 3 O 4 , and Ag/AgCl deposited on the outermost layer. Performance and mechanism research experiments showed that under the optimized conditions of 1 g/L FSNT/Ag/AgCl, pH = 9, temperature of 35℃ and initial MO concentration of 10 mg/L, about 90 % of MO was degraded in 20 min. The mineralization rate was 35.2 % within 120 min. Holes (h + ) and superoxide anions (·O 2 − ) were the main active substances in the whole photocatalytic degradation. FSNT/Ag/AgCl could be recovered by magnet. After 5 repeated experiments, the degradation rate of MO within 100 min still reached 88.5 %. This work provides a recyclable core-shell nanomaterial for faster degradation of dye wastewater.
研究领域:
材料科学
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环境科学
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纳米技术
·
化学工程
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DOI
高影响因子
BIOCATALYSIS AND BIOTRANSFORMATION
·
2016
·
IF 0.892
Danping Qi,Wei Wei,Mingming Diao,Fengxia Lv,Zhaoxin Lu,Xiaomei Bie,Haizhen Zhao
Lipase-catalyzed synthesis of mono- and diesters of 3-O-β-D-galactopyranosyl-sn- glycerol (β-GG) with caproic acid was performed in acetone. The simultaneous production of 1(6’)-monoesters and 1,6’-diesters of β-GG was achieved in this reaction. In order to improve the yield of β-GG esters, four process parameters, enzyme concentration (15 ∼ 25 mg/mL), and substrate molar ratio (caproic acid: β-GG= 1.60 ∼ 2.00 mmol: 0.10 mmol), reaction temperature (40 ∼ 60 °C), and reaction time (8 ∼ 12 h), were optimized via response surface methodology (RSM) employing a three-level-four-variable central composite design. Results showed that enzyme concentration had the most significant (p < 0.0001) effect on the synthesis of β-GG esters. The optimal reaction conditions in acetone were given as follows: Novozyme435 concentration 18.65 mg/mL, molar rate of caproic acid to β-GG 19.46:1, reaction temperature 48 °C, and reaction time 9.83 h. The yield of β-GG esters reached 88.08% under above optimized conditions, which was very close to the predicted value 87.95%. The molar ratio of monoester to diesters was 0.39:0.61. β-GG esters with other fatty acyl chains were synthesized based on the optimized conditions. In vitro antitumor activity indicated that the antitumor activity of β-GG esters was dependent on the nature of fatty acids, such as the length of acyl chain, the degree of saturation, as well as the number of acyl chain.
研究领域:
生物化学
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药物化学
·
癌症研究
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DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 103
·期 6
·3006-3016
·
IF 4.125
Xiaoxiang Han,Jianghui Huang,Jinwang Cai,Chen Yu,Shengchou Jiang,Qing Ye,Shuifen Yang,Weichun Pan,Qiaozhi Zhang,Yanbo Wang
Background As potent antioxidants, anthocyanins can protect the body from free radicals. However, the traditional solvent extraction method has the disadvantages of requiring a high extraction temperature and long extraction time, so it is necessary to develop an efficient extraction method for anthocyanins. Results In this study, the technique of natural deep eutectic solvents (DESs) was applied to extract anthocyanins from purple perilla leaves with the aid of microwave-ultrasonic assisted extraction (MUAE). The response surface methodology (RSM), based on the Box–Behnken design (BBD), predicted the maximum extraction yield of anthocyanins to be 619.62 mg (100 g) −1 under the following conditions: x 1 (ultrasonic extraction power) = 357.25 W, x 2 (time) = 25.62 min, and x 3 (temperature) = 57.80 °C. The biological activity of the extract obtained was evaluated by examining its radical-scavenging effect on 1,1-diphenyl-2-picrylhydrazyl, hydroxyl radical, and superoxide anion radicals. Its bacteriostatic impact was investigated on four typical bacteria: Shewanella putrefaciens ( S. putrefaciens ), Pseudomonas fluorescens ( P. fluorescens ), Escherichia coli ( E. coli ), and Staphylococcus aureus ( S. aureus ). Conclusion The integrated extraction method of DESs with MUAE was efficient, energy-saving, green, and sustainable. © 2022 Society of Chemical Industry.
研究领域:
食品科学
·
化学工程
·
植物化学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2023
·
IF 6
Rong-Shuai Hu,Li Yu,Sai-Ya Zhou,Hui-Fen Zhou,Hai-Tong Wan,Jie-Hong Yang
The natural deep eutectic solvent (NADES) was used as the extraction solvent to extract the four ingredients from Chuanxiong-Honghua. NADES-7 composed of L-proline and lactic acid (1:2) has a better extraction effect compared with traditional solvents. The extraction process was optimized using response surface methodology (RSM) and ant colony algorithm-enabled backpropagation neural network (ACO-BPNN), which were compared with each other. ACO-BPNN has a higher R 2 and predictive value than RSM. The optimal process parameters predicted by the ACO-BPNN were determined to be as follows: solvent-to-material ratio, 8 mL/g; extraction time, 67 min; water concentration, 48%; and extraction temperature, 50 °C. The best predicted comprehensive evaluation value was 4.2634, and the mean value obtained from the experiment was 3.8678. Compared with traditional solvents, the antioxidant activity of NADES-7 extract was better, and the mean value of DPPH, FRAP (ferric reducing antioxidant power), and ABTS assays were 0.1555, 0.1132, and 0.0894 mmol Trolox/g dry weight, respectively. Experimental results show that ACO-BPNN can better optimize the extraction process of many different polar compounds in plant combinations.
研究领域:
药学
·
化工
·
生物技术
·
分析化学
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DOI
高影响因子
Evidence-based Complementary and Alternative Medicine
·
2023
·
IF 2.65
Lei Zhang,Ming-yue Pan,Tao Li,Zhi-min Jin,Zhu Liu,Qiu-yue Liu,Yong Liu,Jia-yuan Ding,Huan Jiang,Xingchen Hou
Quercetin was extracted from Portulaca oleracea L. through biphasic acid hydrolysis to investigate its potential as a suppressor of dipeptidyl peptidase IV (DPP-IV) and its hypoglycemic effect in type 2 diabetic mice. The extraction procedure was optimized utilizing the response surface method (RSM) in a single-factor experimental setting. An extraction efficiency of 0.675% was achieved using the following optimized parameters: 0.064 mol/L vitriol, 1 : 109.155 solid-liquid ratio, and 21.408 min ultrasonication. Overall, findings indicate the effectiveness of quercetin extraction. A mouse model for type 2 diabetes was established to receive oral treatment with various quercetin concentrations for 8 weeks. Fasting blood glucose (FBG) and the DPP-IV activity in the serum were significantly reduced. The weight and insulin levels of the mice in the quercetin group were raised compared to those in the model group ( < 0.01). Quercetin dose-dependently inhibited postprandial blood glucose excursions, as demonstrated by the oral glucose tolerance test. These results confirmed that quercetin has hypoglycemic effects and considerably improves insulin sensitivity via DPP-IV targeting.
研究领域:
药理学
·
生物化学
·
糖尿病研究
·
植物化学
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Kewei Zheng,Yuanyuan Wu,Qingfei Dai,Xiaojun Yan,Yu Liu,Di Sun,Zhongjie Yu,Shuoqi Jiang,Qingbao Ma,Wei Jiang
The objective of this study was to explore the potential of Antarctic krill-derived peptides as α-glucosidase inhibitors for the treatment of type 2 diabetes. The enzymolysis conditions of α-glucosidase inhibitory peptides were optimized by response surface methodology (RSM), a statistical method that efficiently determines optimal conditions with a limited number of experiments. Gel chromatography and LC-MS/MS techniques were utilized to determine the molecular weight (M w ) distribution and sequences of the hydrolysates. The identification and analysis of the mechanism behind α-glucosidase inhibitory peptides were conducted through conventional and computer-assisted techniques. The binding affinities between peptides and α-glucosidase were further validated using BLI (biolayer interferometry) assay. The results revealed that hydrolysates generated by neutrase exhibited the highest α-glucosidase inhibition rate. Optimal conditions for hydrolysis were determined to be an enzyme concentration of 6 × 10 3 U/g, hydrolysis time of 5.4 h, and hydrolysis temperature of 45 °C. Four peptides (LPFQR, PSFD, PSFDF, VPFPR) with strong binding affinities to the active site of α-glucosidase, primarily through hydrogen bonding and hydrophobic interactions. This study highlights the prospective utility of Antarctic krill-derived peptides in curtailing α-glucosidase activity, offering a theoretical foundation for the development of novel α-glucosidase inhibitors and related functional foods to enhance diabetes management.
研究领域:
生物化学
·
药理学
·
食品科学
·
糖尿病研究
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）