MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Dongmei Liu,Xuejia Chen,Hui Zeng,Chang Liu,Biyu Tang,Yan Li,Lishan Zhang,Min Zhang
In this work, a novel, low-cost soap-film sensor for detecting ammonia in environmental water and saliva was developed. A 3D printed circular frame with two stainless steel electrodes was implemented as a carrier for a soap film. The soap film was manually generated by pulling the 3D printed frame from a mixture solution containing 20.7 g/L gelatin, 241.4 mL/L Glycerol, 34.5 mL/L Triton X-100 and 100 mM boric acid . The conductivity of the soap film was measured with a lab-made detector. For liquid sample analysis, sample and NaOH solution were mixed in a reagent bottle as the gas-diffusion vessel (GDV). The released ammonia from the reaction was then diffused into the headspace of the GDV and absorbed by the soap film. The conductivity increasing rate of the soap film was used for quantifying the ammonium concentration in sample. The analytical performance parameters were demonstrated, including a linear range of 0–500 μM, a relative standard deviation of 3.2 % ( n = 10), and a limit of detection of 14 μM (0.2 mg N/L). This method showed a 90–110 % recovery rate for saliva and water samples. Compared to a reference indophenol blue method, the relative deviations of two method were less than 7.4 % ( n = 33). The proposed method is easy to operate, and the device itself is compact and low cost. The soap-film sensor enables rapid renewal to avoid contamination.
研究领域:
环境科学
·
分析化学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 12
·1-8
·
IF 5.705
Lin Haizhu,Chen Hemei,Shao Xi,Deng Chunhui
A zirconium(IV)-based metal organic framework (Zr-MOF) was deposited on polydopamine-coated silica microspheres to form microspheres of type SiO 2 @PDA@Zr-MOF. These were packed into capillary columns for enrichment of phosphopeptides. The column was off-line coupled to both matrix-assisted laser desorption/ionization time of flight mass spectrometry and LC-ESI-MS/MS. The method has a detection limit as low as 4 fmol of β-casein digest and a selectivity as high as 1:1000 (molar ratio of β-casein and BSA digest). It was applied to the analysis of human saliva. In total, 240 endogenous phosphopeptides were identified in only 25 μL human saliva.
A zirconium-based metal organic framework (Zr-MOF) was modified outside of polydopamine-coated silica microspheres to form microspheres named SiO 2 @PDA@Zr-MOF. Then they were packed in capillary columns for selective enrichment of phosphopeptides via interaction between Zr-O clusters and phosphate groups. The pre-concentration resulted in a better detection of phosphopeptides by mass spectrometry. Tris: Tris(hydroxymethyl)aminomethane; DMF: Dimethyl Formamide; Zr-MOF: Zirconium(IV)-organic framework; MOAC: Metal oxide affinity chromatography.
研究领域:
分析化学
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生物医学
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DOI
PubMed
高影响因子
CHEMICAL PHYSICS LETTERS
·
2023
·
IF 2.8
Huaqing Lin,Ming Yin,Chenjie Yang,Dongpo Xu,Chunhui Deng
Laser desorption/ionisation mass spectrometry (LDI-MS) can be used as an effective detection method for the analysis of more complex samples. However, the organic matrix of conventional LDI-MS produces large background interference and poor signal results, which hinders its application in metabolomics. Surface reconstruction can improve the performance of LDI-MS. In this study, mesoporous NiO@Pt nanofibre membranes were synthesised by a simple and efficient electrostatic spinning strategy and used as a matrix to significantly increase the intensity of mass spectrometry peaks. Metabolomic analysis of saliva samples from 18 smokers and 15 non-smokers was able to clearly distinguish the differences in salivary metabolism between smokers and non-smokers and showed good results in the qualitative analysis of salivary metabolism in smokers. Our study provides a rapid and convenient method for differentiating differences in salivary metabolism in smokers.
研究领域:
分析化学
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代谢组学
·
医学诊断
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Yonglei Wu,Haizhu Lin,Zixing Xu,Yan Li,Zongbao Chen,Chunhui Deng
In this work, for the first time, L -cysteine ( L -Cys), a zwitterionic hydrophilic compound containing abundant amino and carboxyl was modified on silica microspheres for the fabrication of SiO 2 @ L -Cys capillary packed column. Since the SiO 2 @ L -Cys capillary packed column possessed great hydrophilicity brought by L -cysteine, they could be utilized to enrich glycopeptides with a low detection limit of 5 fmol μL −1 HRP digests as well as a good selectivity of the mixture of HRP and BSA digests up to a molar ratio of 1:50. The capillary packed column were further applied into the glycosylation analysis of human saliva and 69 glycopeptides were identified from 2 μL human saliva digests.
研究领域:
分析化学
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生物化学
·
蛋白质组学
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 11
·1-11
·
IF 5.3
Lu Feng,Li Limao,Shen Kang,Qian Yayun,Zhang Pengfei,Yang Yan,Zhu Qunshan,Huang Yong,Yan Chunxiang,Wei Wei
A Raman-active boronate modified surface-enhanced Raman scattering (SERS) microporous array chip based on the enzymatic reaction was constructed for reliable, sensitive, and quantitative monitoring of D-Proline (D-Pro) and D-Alanine (D-Ala) in saliva. Initially, 3-mercaptophenylboronic acid (3-MPBA) was bonded to Au-coated Si nanocrown arrays (Au/SiNCA) via Au–S bonding. Following this, H 2 O 2 obtained from D-amino acid oxidase (DAAO)-specific catalyzed D-amino acids (D-AAs) further reduced 3-MPBA to 3-hydroxythiophenol (3-HTP) with a new Raman peak at 882 cm −1 . Meanwhile, the original characteristic peak at 998 cm −1 remained unchanged. Therefore, the I 882 /I 998 ratio increased with increasing content of D-AAs in the sample to be tested, allowing D-AAs to be quantitatively detected. The Au/SiNCA with large-area periodic crown structure prepared provided numerous, uniform “hot spots,” and the microporous array chip with 16 detection units was employed as the platform for SERS analysis, realizing high-throughput, high sensitivity, high specificity and high-reliability quantitative detection of D-AAs (D-Pro and D-Ala). The limits of detection (LOD) were down to 10.1 µM and 13.7 µM throughout the linear range of 20–500 µM. The good results of the saliva detection suggested that this SERS sensor could rapidly differentiate between early-stage gastric cancer patients and healthy individuals. Graphical Abstract
研究领域:
生物化学
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医学诊断
·
分析化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
·
2019
·Vol. 166
·期 6
·B524
·
IF 3.12
Yingchun Li,Zedong Li,Hao Liu,Siyue Chen,Xiaojin Guo,Min Lin,Fei Li
Point-of-care testing (POCT) of neurotransmitters, such as dopamine (DA) and 5-hydroxytryptamine (5-HT), can be used for early diagnosis of neurological diseases. Among various POCT platforms, electrochemical method-based platforms have attracted increasing interest owning to their high detection sensitivity and specificity as well as fast response time. In this work, we developed a portable electrochemical POCT platform for detection of neurotransmitters, which is based on integration of a three-dimensional (3D) gold nanoparticles/carbon nanotubes (AuNPs/CNTs) sponge synthesized by a simple in-situ growth and eco-friendly ice-templating method modified screen-printed electrode with a miniaturized USB electrochemical analyzer. We demonstrated the good performance of the portable electrochemical platform for detection of two typical neurotransmitters, i.e., DA and 5-HT, with detection sensitivities of 1.02 μA μM−1 cm−2 and 0.55 μA μM−1 cm−2, and detection limits of 0.06 μM and 0.30 μM for DA and 5-HT, respectively. We further verified the feasibility and practicability of the platform by simultaneous detection of DA and 5-HT in spiked saliva. The developed portable electrochemical platform provides a simple and user-friendly way for early diagnosis of neurological diseases in the future, especially at point of care.
研究领域:
生物医学工程
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神经科学
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电化学分析
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即时检测技术
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分子诊断
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纳米生物技术
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DOI
高影响因子
NANO
·
2020
·
IF 1.566
Mei Wang,Fang Liu,Zhifeng Zhang,Erchao Meng,Feilong Gong,Feng Li
A new noninvasive glucose sensor is developed based on Co 3 O 4 particles (Co 3 O 4 NPs), which are synthesized by a single-step hydrothermal method with uniform structure and size. The electrochemical measurements reveal that the device exhibits outstanding performance for glucose detection, achieving a maximal sensitivity of 2495.79 μ A mM − 1 cm − 2 with a high R 2 of 0.99575, a ultra-low detection limit of 9.3 nM with a signal-to-noise of 3 and linear range up to 3 mM. The noninvasive glucose sensor can respond swiftly and selectively due to the high electrocatalytic activity of Co 3 O 4 NPs. The sensor also shows its high sensitivity and selectivity in detecting glucose levels in human blood serum and saliva sample, confirming the application potential of Co 3 O 4 NPs in noninvasive detection of glucose.
研究领域:
材料科学
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电化学
·
纳米技术
·
生物传感器
·
生物医学工程
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2021
·
IF 4.759
Xi-Ling Li,Yu Han,Yuan Huang,Xiaoqi Sun,Shuyun Xiao,Jun Zhe Min
We developed a novel fluorescent chiral probe, DBD-trans-2-methyl-L-proline (DBD-M-Pro), which can be used to target recognition of amino functional groups using chiral resolution. To investigate the chiral resolution efficiency, 20 chiral amino enantiomers (19 DL-amino acids and phenylethylamine) were labeled using ultra-performance liquid chromatography (UPLC) with a fluorescence (FL) system. Diastereomers were formed by the reactions of DBD-M-Pro with enantiomers of amino functional groups at 60 °C for 60 min and detected on a BEH C18 column (100 × 2.1 mm, 1.7 μm). Gradient elution of 10 mM ammonium acetate with 0.05% formic acid (FA) aqueous solution and 0.1% FA acetonitrile or 0.1% FA methanol solution was performed at an excitation wavelength (Ex) 460 nm and emission wavelength (Em) 550 nm. Each resulting derivative of D- and L- type was effectively separated. The results showed that the resolution (Rs) of 17 amino acids and phenylethylamine (PEA) in the range of 1.59–24.11, except for histidine (His) (Rs = 1.32) and serine (Ser) (Rs = 1.47), achieved completely separation. The DBD-M-Pro chiral probe has a robust chiral selectivity for D-amino acids. Furthermore, a new method for the simultaneous determination of six DL-amino acids (Pro, Val, Trp, Phe, Leu, Lys) in human saliva was developed. The proposed method showed resolution values of 1.78–16.38, and an excellent linear relationship was obtained in the range of 2.5–500 pmol ( R 2 ≥ 0.9990). The limit of detection (S/ N = 3) ranged from 0.5 to 3.75 pmol. The intra-day and inter-day coefficient of variation (CV) were within the range of 1.75–11.73%. The average addition recoveries in saliva ranged from 95.99 to 106.97%. The methodology was used to determine the content of DL-amino acids and the D/L-amino acid ratio in the saliva of 40 healthy volunteers (15 males and 25 females), as well as evaluating the differences between men and women. Our study suggests that the DBD-M-Pro chiral probe could be an effective tool for screening potential D-amino acid biomarkers in disease.
研究领域:
分析化学
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生物化学
·
医学诊断
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Yuxuan Li,Yuanjie Shan,Chenglong Yu,Xi-Ling Li,Jun Zhe Min
Smoking is detrimental to health. However, the potential of dynamic changes in chiral thiol compounds in human saliva, both before and after smoking, as indicators of oxidative stress in vivo remains unexplored. A novel ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC HRMS) method was developed for the simultaneous separation of four DL-thiol compounds in human saliva, utilizing a new ( R )-(5-(3-isothiocyanatopyrrolidin-1-yl)-5-oxopentyl (NCS-OTPP) pre-column derivatization approach. Chromatographic separation was carried out using a YMC Triart C18 column (2.0 × 150 mm, 1.9 µm), with isocratic elution of 0.1 % formic acid aqueous solution and 0.1 % formic acid methanol-acetonitrile (7:3, v/v ) at a ratio of 65:35. Separation of DL-glutathione (GSH), DL-cysteine (Cys), DL-homocysteine (Hcy), and γ-glutamylcysteine (γ-Glu-Cys) achieved resolution degrees of 1.60–1.89, with complete separation. Linear calibration was observed (R 2 ≥ 0.9992) within a range of 0.5–20 µM, with detection limits spanning 2.50–14.40 fmol. The intraday and interday precisions ranged from 0.33 % to 9.64 %, while average spiked recoveries were 92.15–106.22 %. This method was successfully applied to assess the four DL-thiol compounds in the saliva of 10 smokers and 10 non-smokers at different time points. Results revealed significantly higher concentrations of the DL-thiol compounds in the saliva of smokers compared to non-smokers, with significant differences for l-GSH, DL-Cys, and l-Hcy ( p < 0.05), returning to baseline levels within 15 min. Dynamic monitoring curves before and after smoking exhibited consistent trends with the fitted curves. This study introduces a new method for monitoring dynamic changes in DL-thiol compounds in saliva and offers insight into potential subhealth indicators linked to abnormal chiral thiol metabolism in vivo.
研究领域:
分析化学
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生物化学
·
临床化学
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质谱学
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公共卫生
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）