JOURNAL OF FOOD PROTECTION
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2017
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IF 1.417
Dan Hu,Xu Xu,Tian Cai,Wei-Ying Wang,Chun-Jie Wu,Li-Ming Ye
ABSTRACT A rapid and sensitive analytical method based on high-performance liquid chromatography–tandem mass spectrometry was developed and validated for the determination of isopyrazam (IZM) and azoxystrobin (AZT) in cucumbers. A modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method was used as the pretreatment procedure. The samples were extracted with acetonitrile and cleaned up with octadecylsilyl silica (C18) and graphite carbon black. The proposed method resulted in satisfactory recovery of IZM and AZT (91.48 to 114.62%), and relative standard deviations were less than 13.1% at fortification concentrations of 1, 20, and 500 μg kg −1 ( n = 3). The limits of quantification for IZM and AZT were 0.498 and 0.499 μg kg −1 , respectively, which are far below the maximum residue level (0.5 mg kg −1 ) established for this type of sample. Matrix effects were also evaluated. This study established a sensitive and fast method for the detection of IZM and AZT in cucumber samples.
研究领域:
食品科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Catalysts
·
2021
·Vol. 11
·期 10
·1169
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IF 4.146
Feng Chen,Dezheng Yang,Feng Yu,Yang Kun,Ying Song
In this work, the azoxystrobin removal in water by using a micro-size discharge array was investigated, and the removal efficiency can reach as high as 98.1% after 9 min plasma treatment as well as the energy utilization being only 0.73 g/(kW·h). Based on the relationship between the generation of gas bubbles and parameters of gas-liquid discharge, it was found that the variation of applied voltage, gas flow rate and initial solution temperature could cause particle number change, mass transfer rate change and the mass transfer time change, which significantly affected the practical applications at last. The experimental results indicated that when gas flow rate was 0.7 SLM (Standard Liter per Minute) and the initial solution temperature was 297 K with the applied voltage of 8 kV and discharge frequency of 6 kHz, the removal efficiency of azoxystrobin achieved maximum. Based on the analysis results of liquid mass spectrometry, the removal pathways of azoxystrobin were supposed by the decomposed by-products. Toxicity tests indicated that the decomposed products were safe and non-toxic. So, this study may reveal an azoxystrobin degradation mechanism and provide a safe, reliable and effective way for azoxystrobin degradation.Keywords:non-thermal plasma;azoxystrobin;micro-discharge array reactor;mass transfer;degradation
研究领域:
环境科学
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农业科学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Xuanjun Guo,Ruihua Zhang,Qian Jin,Niannian Cao,Jingjing Shi,Xingxing Zong,Xuejun Chen,Chen Wang,Xuefeng Li,Sen Pang,Liqin Li
Azoxystrobin, a strobilurin widely used to control rice diseases, has raised concerns about possible adverse effects on aquatic ecosystems. At present, very little is known about the effects of azoxystrobin on courtship and aggressive behavior and the potential underlying mechanisms. In the present study, after exposing adult male and female zebrafish to worst-case scenario concentrations of azoxystrobin (0, 2 μg/L, 20 μg/L, and 200 μg/L) for 42 d, we observed a decrease in courtship behavior and an increase in aggressive behavior in both male and female zebrafish. In addition, to elucidate the molecular mechanism of the behavioral effects of azoxystrobin, we quantified the changes in the concentrations of kisspeptin, 5-HT, GnIH, and their corresponding receptor mRNA expression in the brain. The results showed that 200 μg/L azoxystrobin decreased the concentrations of kisspeptin and increased the concentration of GnIH in both male and female zebrafish brain. In addition, azoxystrobin also significantly reduced 5-HT concentration in female zebrafish brain. Further investigation revealed that altered courtship and aggressive behavior were associated with the expression levels of genes (kiss1, kiss2, gnrh3, gnrhr3, 5ht1a, and 5ht2a) involved in kisspeptin-GnIH signaling pathway. In conclusion, our study suggested that azoxystrobin may impair courtship and aggressive behavior in zebrafish by interfering with the kisspeptin-GnIH signaling pathway, which may have more profound effects on natural zebrafish populations.
研究领域:
环境毒理学
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行为生物学
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神经内分泌学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2018
·
IF 3.974
Benben Du,Zhenyan Zhang,Wanyue Liu,Yizhi Ye,Tao Lu,Zhigao Zhou,Yan Li,Zhanyu Fu,Haifeng Qian
Azoxystrobin (AZ) is an effective broad-spectrum fungicide . Due to its extensive application, AZ is detectable in aquatic ecosystems and thus influences aquatic organisms. In this study, the acute toxicity (96 h) of AZ at concentrations of 1.0 mg/L and 5.0 mg/L on the diatom Phaeodactylum tricornutum were examined. At the tested concentrations, AZ significantly inhibited P. tricornutum growth and destroyed its cellular structure. Furthermore, the mechanisms of AZ-induced toxicity on P. tricornutum changed as the exposure time extended. Forty-eight hours after exposure, AZ inhibited P. tricornutum growth primarily via inducing oxidative stress , which increased the activity of two main antioxidant enzymes, superoxide dismutase and peroxidase, and inhibited energy metabolism. However, after 96 h of treatment, the decline in the photosynthetic capacity of P. tricornutum demonstrated that the photosystem was the main AZ target. The pigment content and expression levels of genes related to photosynthetic electron transfer reactions were also significantly decreased. The present study describes AZ toxicity in P. tricornutum and is very valuable for assessing the environmental risk of AZ.
研究领域:
环境科学
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毒理学
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水生生态学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2013
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IF 2.487
Pei Liang,Guojiao Liu,Fang Wang,Wanting Wang
A novel method, ultrasound-assisted surfactant-enhanced emulsification microextraction with solidification of floating organic droplet (UASEME-SFOD), has been developed for the extraction of four strobilurin fungicides (kresoxim-methyl, picoxystrobin, pyraclostrobin and trifloxystrobin) in fruit juices. In the UASEME-SFOD technique, Tween 80 was used as emulsifier, and 1-undecanol was used as extraction solvent without using any organic dispersive solvent. Several parameters that affect the extraction efficiency, such as the kind and volume of extraction solvent, the type and concentration of the surfactant, extraction time, extraction temperature and salt addition were investigated and optimized. Under the optimum extraction condition, the method yields a linear calibration curve in the concentration range from 5 to 10,000 ng mL −1 for the targeted analytes with the correlation coefficient ranging from 0.9991 to 0.9998. The enrichment factors were in the range between 95 and 135, and the limits of detection of the method were 2–4 ng mL −1 . The fruit juice samples were successfully analyzed using the proposed method, and the relative recoveries at fortified levels of 50 and 100 ng mL −1 were in the range of 82.6–97.5%.
研究领域:
分析化学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2022
·
IF 7.129
Tianxiu Qiu,Huan Wang,Lei Liu,Jiong Chen
Azoxystrobin (AZ) is one of the most widely used strobilurin fungicides in the world, and its residue has seriously endangered aquatic ecological security. Our previous data showed that AZ exposure may reduce the resistance of fish to rhabdovirus infection in aquatic environment. Here, we further reported a potential long-term adverse effect of AZ exposure on the antiviral and immunosuppressive recovery in fish, and observed that mitochondrial dynamic balance was disturbed by AZ in which excessive mitochondrial fission occurred in response to decreased ATP levels. When a recovery operation was performed in AZ-exposed cells and fish, infectivity of our model virus, spring viraemia of carp virus (SVCV), was significantly decreased in vitro (using the epithelioma papulosum cyprini [EPC] fish cell line) and in vivo (using zebrafish) in a time-dependent manner. Also, the expression of eight innate antiviral immune genes ( IFNs , ISG15 , MX1 , RIG-I , IRF3 , Nrf2 and HO-1 ) showed a similar change to SVCV replication between the longer exposure period and the expression recovery. Additionally, AZ facilitated horizontal transmission of SVCV in a static cohabitation challenge model, predicting the increase of the potential for the viral outbreak. Therefore, our data suggest that long-term effect of AZ on irreparable impairment in fish made AZ residue potentially greater for ecological risks.
研究领域:
水生毒理学
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免疫学
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病毒学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2018
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IF 3.974
Xiaoxin Chen,Sheng He,Xiaolu Liu,Jiye Hu
Using sequential soil and liquid culture enrichments with cyprodinil as the sole carbon source, a Gram-negative cyprodinil-degrader from cyprodinil-polluted agricultural soil was isolated. The sequencing analysis of 16 S rRNA indicated that the strain showed 99% homology to Acinetobacter sp. The strain could effectively degrade cyprodinil at the neutral condition. At the initial concentrations of 10, 20, 50, 100, 150 and 200 mg L −1 in minimal medium, cyprodinil was degraded by 10, 20, 49.3, 64.2, 57 and 24 mg L −1 within 14 days, respectively. Two metabolites (4-cyclopropyl-6-methyl-2-pyrimidpyridine amine and monohydroxylated para-substitution) were identified using high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS/MS). A biodegradation pathway involving imines hydrolysis and monohydroxyl substitution on benzene ring was proposed on basis of the identified metabolites. Acinetobacter sp. would have a potential application in bioremediation of cyprodinil-contaminated soil, and the strain might have important implications in detoxification and bioremediation of pyrimidine analogues.
研究领域:
环境微生物学
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生物修复
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农药降解
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土壤科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2023
·
IF 8.9
Guoyan Qin,Qi Zhang,Ziyao Zhang,Yiling Chen,Jichao Zhu,Yaohui Yang,W.J.G.M. Peijnenburg,Haifeng Qian
Increasing knowledge of the impacts of pesticides on soil ecological communities is fundamental to a comprehensive understanding of the functional changes in the global agroecosystem industry. In this study, we examined microbial community shifts in the gut of the soil-dwelling organism Enchytraeus crypticus and functional shifts in the soil microbiome (bacteria and viruses) after 21 d of exposure to difenoconazole, one of the main fungicides in intensified agriculture. Our results demonstrated reduced body weight and increased oxidative stress levels of E. crypticus under difenoconazole treatment. Meanwhile, difenoconazole not only altered the composition and structure of the gut microbial community, but also interfered with the soil-soil fauna microecology stability by impairing the abundance of beneficial bacteria. Using soil metagenomics , we revealed that bacterial genes encoding detoxification and viruses encoding carbon cycle genes exhibited a dependent enrichment in the toxicity of pesticides via metabolism. Taken together, these findings advance the understanding of the ecotoxicological impact of residual difenoconazole on the soil-soil fauna micro-ecology, and the ecological importance of virus-encoded auxiliary metabolic genes under pesticide stress.
研究领域:
环境科学
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微生物学
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生态学
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农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2022
·
IF 8.943
Nan Fang,Changpeng Zhang,Haoze Hu,Yanjie Li,Xiangyun Wang,Xueping Zhao,Jinhua Jiang
Studies have shown that kresoxim-methyl (KM) and other strobilurin fungicides have toxic effects on aquatic organisms. However, the potential deleterious effects of kresoxim-methyl (KM) on adult zebrafish regarding the ecological risk of environmental concentration remain unclear. Here, the histology and untargeted metabonomics was used to investigate the adverse effect on female zebrafish after exposure to KM at environmental concentration, aquatic life benchmark and one-half LC 50 of adult zebrafish. Results demonstrated KM affected zebrafish liver, ovary and intestine development, blurred the boundary between hepatocytes or caused hepatic vacuoles, increased the percentage of perinucleolar oocyte and cortical alveolus oocyte, decreased intestinal goblet cells and disturbed villus and wall integrity after 21 d exposure. Metabonomics showed different concentrations of KM simultaneously influenced the metabolites annotated to vitamin digestion and absorption, serotonergic synapse, retinol metabolism, ovarian steroidogenesis and arachidonic acid (AA) metabolism in zebrafish liver. Results showed the decreased triglyceride and cholesterol levels, as well as the metabolic alterations in amino acid, lipid, vitamin and retinol metabolism caused by KM, might disturb the energy supply for normal liver development and oocyte maturation. In addition, KM altered the transcription of Tdo2a , Tdo2b , Ido1 , Cxcl8b , Cyp7a , Cyp11a , Cyp11b , Cyp17a , Cyp19a , Hsd3β , Hsd17β , Pla2 , Ptgs2a and Ptgs2b , the level of TG, TC, MDA, IFN, IL6 and Ca 2+ , and the activity of CAT , SOD Ca 2+ -ATPase in zebrafish liver. Moreover, cytoscape analysis suggested the disturbed AA metabolism caused by KM, might interconnect multiple metabolic pathways to share implicated function in the regulation of oocyte maturation and immune response. Current study brought us closer to an incremental understanding of the toxic mechanism of KM on adult zebrafish, indicated there was crosstalk among different regulatory pathways to regulate the metabolic disorders and biologically hazardous effects induced by KM.
研究领域:
环境科学
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毒理学
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水生生物学
·
代谢组学
✓ 已验证
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2023
·
IF 6.8
Xuanjun Guo,Ruihua Zhang,Changsheng Li,Manman Duan,Niannian Cao,Qian Jin,Xuejun Chen,Liqin Li,Xuefeng Li,Sen Pang
A widely applied pesticide of azoxystrobin, is increasingly detected in the water environment. Concern has been raised against its potential detriment to aquatic ecosystems. It has been shown that exposure to azoxystrobin interfere with the locomotor behavior of zebrafish larvae. This study aims to investigate whether exposure to environmental levels of azoxystrobin (2 μg/L, 20 μg/L, and 200 μg/L) changes the behavior of male adult zebrafish. Herein, we evaluated behavioral response (locomotor, anxiety-like, and exploratory behaviors), histopathology, biochemical indicators, and gene expression in male adult zebrafish upon azoxystrobin exposure. The study showed that exposure to azoxystrobin for 42 days remarkably increased the locomotor ability of male zebrafish, resulted in anxiety-like behavior, and inhibited exploratory behavior. After treatment with 200 μg/L azoxystrobin, vasodilatation, and congestion were observed in male zebrafish brains. Exposure to 200 μg/L azoxystrobin notably elevated ROS level, MDA concentration, CAT activity, and AChE activity, while inhibiting SOD activity, GPx activity, ACh concentration, and DA concentration in male zebrafish brains. Moreover, the expression levels of genes related to the antioxidant, cholinergic, and dopaminergic systems were significantly changed. This suggests that azoxystrobin may interfere with the homeostasis of neurotransmitters by causing oxidative stress in male zebrafish brains, thus affecting the behavioral response of male zebrafish.
研究领域:
环境科学
·
毒理学
·
神经科学
·
水生生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Min Wang,Yajie Yue,Xiaoning Wei,Jinyuan Zhang,Xinyuan Bi,Liyan Jia,Xu Jing
Strobilurin fungicides are widely used pesticides globally; however, their residues pose a risk to environmental safety and human health. This study aimed to develop a rapid and green method for detecting strobilurin fungicide residues. Chitosan and magnetic deep eutectic solvent-based emulsive liquid-liquid microextraction method combined with high-performance liquid chromatography was developed to determine strobilurin fungicides in water samples. A high-concentration oil-in-water emulsion containing chitosan solution and extractant was formulated, followed by the addition of samples to form a low-concentration oil-in-water emulsion to complete the rapid pretreatment, therefore solving the limitation of emulsive liquid-liquid microextraction in the inapplicability of extractants. Traditional microextraction required toxic solvents and complex equipment to facilitate the dispersion of extractants, whereas this study used only chitosan solution to aid in emulsifying extractants. The new green magnetic deep eutectic solvent synthesized by tetraoctylammonium bromide, ferric chloride, and oleic acid was used instead of toxic extractants. An external magnetic field assisted the extractant phase collection, which shortened the separation time. Under optimal conditions, the recovery of this method was 83.3 %–107.4 %, the limit of detection was 0.0010–0.0021 mg L −1 , and the coefficient of determination was >0.994. This rapid, green, simple, and efficient chitosan-based microextraction method could detect strobilurin fungicide residues in water samples because of its enhanced compatibility with hydrophobicity extractants.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Yuxin Wang,Yulin Wang,Jizhen Fu,Suzhen Li,Yawen Zhang,Xiaojiao Zheng,Jin Liu,Li Li,Xu Jing
In this study, a rapid and simple semi-automatic emulsification liquid-liquid microextraction technique based on deep eutectic solvents was developed for determining strobilurin fungicides in food samples using high-performance liquid chromatography. Novel deep eutectic solvents formed as extractants by combining quaternary phosphates and fatty acids, eliminating the need for toxic solvents in liquid-phase microextraction. The extraction process was facilitated by an electric micropipette, which emulsified and injected the deep eutectic solvent-water mixture directly into the sample. The semi-automated approach enhanced extraction speed and efficiency while minimizing manual error. The sample and deep eutectic solvent mixture were emulsified immediately and separated within 15 min of standing. Only an electronic micropipette was used to achieve extraction and separation. The method demonstrated a linear range of 2–200 μg L −1 with R 2 > 0.996 and a limit of detection of 0.6 μg L −1 . Recovery ranged from 73.0 % to 100.1 %, with relative standard deviations from 0.4 % to 6.1 %. The greenness of the method was validated through multiple assessment tools, including the Green Analytical Procedure Index, Analytical GREEnness metric for sample preparation, and Sample Preparation Metric of Sustainability. The emulsification liquid-liquid microextraction procedure, based on innovative solvents and electric micropipette technology, proved successful for application in water, juice, and vinegar samples and showed potential for broader use in pesticide residue analysis in food.
研究领域:
分析化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）