BIOTECHNOLOGY LETTERS
·
2023
·Vol. 45
·期 7
·873-883
·
IF 2.7
Zhang Zhenxing,Meng Min,Wu Qiong,Kim Jeong-Han,Zhu Yongzhe
Quinalphos is a long-term, wide-spectrum organophosphate insecticide with residual problems in the natural environment. Cunninghamella elegans ( C. elegans ) is a member of Mucoromycotina. Since the degradation products of its exogenous compounds are similar to those of mammals, it is often used to simulate the metabolism pathways of mammals. In this study, the detailed metabolic pathways of quinalphos were investigated with C. elegans . Quinalphos was degraded by 92% in 7 days, while ten metabolites were produced. The metabolites were analyzed and identified by GC–MS. To determine the responsible enzymes in quinalphos metabolism, piperonyl butoxide (PB) and methimazole included in the culture flasks, and the kinetic responses of quinalphos and its metabolites by C. elegans were measured. Results indirectly demonstrated that cytochrome P450 monooxygenases were involved in the metabolism of quinalphos, but that methimazole inhibited the metabolism less efficiently. Comprehensive metabolic pathways can be deduced from the detailed analysis of metabolite profiles in control and inhibitor assays.
研究领域:
环境科学
·
微生物学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2012
·Vol. 137
·期 5
·1252-1258
·
IF 4.23
Na Gao,Jianwei Dong,Ming Liu,Baoan Ning,Chaonan Cheng,Chun Guo,Caihong Zhou,Yuan Peng,Jialei Bai,Zhixian Gao
A quartz crystal microbalance (QCM) sensor based on molecularly imprinted ultra-thin films was developed for detecting profenofos in real samples. Films prepared by physical entrapment (MIP-A) and in situself-assembly (MIP-B) were compared. The results indicated that the best sensing signal was obtained through the in situself-assembly method. The QCM sensor chip was pretreated with 11-mercaptoundecanoic acid (MUA) to form a self-assembled monolayer (SAM), and then polymer films were immobilized directly on the SAM using surface-initiated radical polymerization. In this paper, all detection experiments were taken in air. The reaction was processed in solution, and the electrode was washed with deionized water and dried with N2 before QCM measurement. The film was characterized by a scanning electron microscope (SEM), AC impedance and cyclic voltammetry. Analysis of the QCM response in the presence of different concentrations of profenofos showed a good linear correlation during 1.0 × 10−8 to 1.0 × 10−5 mg mL−1 (y = 5log x + 42.5, R = 0.9960) and 1.0 × 10−5 to 1.0 × 10−3 mg mL−1 (y = 25.86log x + 146, R = 0.9959), respectively. The MIP-QCM sensor was used to detect profenofos in tap water, and showed good recovery and repeatability.
研究领域:
分析化学
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环境科学
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材料科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 9
·1-12
·
IF 6.232
Liu Lixia,Yang Minli,He Muyi,Liu Tong,Chen Fengming,Li Yinlong,Feng Xuesong,Zhang Yuan,Zhang Feng
A novel magnetic dual-template molecularly imprinted polymer (DMIP) was prepared with methyl-parathion and quinalphos as templates. For comparison, a series of single-template polymers with only methyl-parathion (MPMIP) or quinalphos (QPMIP) as template as well as a non-imprinted polymer (NIP) in the absence of the template, were synthesized using the same procedure of DMIP. The obtained MIPs were characterized by scanning electron microscopy(SEM), Fourier transform infrared (FT-IR) spectroscopy, vibrating sample magnetometer (VSM), and X-ray diffraction (XRD). The properties including kinetic effect, thermodynamic effect, selectivity, and reusability of MIPs were investigated . Only DMIP possessed high affinity and good recognition for all twelve OPPs including quinalphos, isazophos, chlorpyrifos-methyl, chlorpyrifos, methidathion, triazophos, profenofos, fenthion, fenitrothion, methyl-parathion, parathion, and paraoxon in comparison to MPMIP, QPMIP, or NIP. Moreover, DMIP was used as magnetic solid phase extraction (MSPE) sorbent for the pre-concentration of twelve OPPs in cabbage samples. The developed DMIP-MSPE-GC-MS method showed high sensitivity, low LODs (1.62–13.9 ng/g), fast adsorption equilibrium (10 min), and acceptable spiked recoveries (81.5–113.4%) with relative standard deviations (RSD) in the range 0.05–7.0% ( n = 3). The calibration plots were linear in the range 10–800 ng/mL with coefficients of determination ( R 2 ) better 0.99 for all twelve compounds. These results suggest that the DMIP is applicable for rapid determination and high throughput analysis of multi-pesticide residues.
Graphical abstract
研究领域:
分析化学
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环境科学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Zheng Wang,Hui Li,Chuang Jiang,Wenya Liu,Siyu Zhang,Yibin Zhou,Kang Liu,Yaqing Xiao,Ruyan Hou,Xiaochun Wan,Yingnan Liu
Herein, a novel dual-mode probe for organophosphorus pesticides (OPs) colorimetric and photothermal detection was developed based on manganese modified porphyrin metal–organic framework (PCN-224-Mn). PCN-224-Mn had excellent oxidase-like activity and oxidized colorless 3,3,5,5-tetramethylbenzidine (TMB) to blue–green oxidation state TMB (oxTMB), which exhibited high temperature under near-infrared irradiation. l -ascorbate-2-phosphate was hydrolyzed by acid phosphatase to produce ascorbic acid, which weakened colorimetric and photothermal signals by impacting oxTMB generation. The presence of OPs blocked the production of ascorbic acid by irreversibly inhibiting the activity of acid phosphatase, causing the restoration of chromogenic reaction and the increase of temperature. Under the optimal conditions, the probe showed a good linear response to OPs in the concentration range of 5 ∼ 10000 ng/mL, using glyphosate as the analog. The detection limits of glyphosate in colorimetric mode and photothermal mode were 1.47 ng/mL and 2.00 ng/mL, respectively. The probe was successfully used for sensitive identification of OPs residues in tea, brown rice, and wheat flour. This work proposes a simple and reliable colorimetric/photothermal platform for OPs identification, which overcomes the problem that single-mode detection probes are susceptible to external factors, and has broad application potential in the field of food safety.
研究领域:
分析化学
·
环境科学
·
食品科学
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Qianqian Lu,Lingling Guo,Xinxin Xu,Hua Kuang,Liqiang Liu,Chuanlai Xu,Maozhong Sun
The mass production and use of organophosphorus pesticides (OPs) have led to a threat to human health. Therefore, establishing a sensitive, rapid, and high-throughput detection method is of great importance. In this study, computer-aided molecular design was firstly applied to design the specific haptens of phorate (PHO), fenthion (FEN), and profenofos (PRO), and high-performance monoclonal antibodies against PHO, FEN, and PRO were prepared. On this basis, a multiplex fluorescent microsphere immunochromatographic assay (FM-ICA) was developed for simultaneous detection of three OPs in cowpea and orange samples. The quantitative detection ranges of PHO, FEN, and PRO were 9.039–140.129, 6.686–108.613, and 5.215–71.479 μg/kg, respectively, in cowpeas; and 7.120–121.566, 3.592–92.185, and 3.579–60.081 μg/kg, respectively, in oranges. The results of recovery and real sample testing showed that the multiplex FM-ICA was rapid and accurate, making it suitable for the on-site testing of a large number of samples.
研究领域:
分析化学
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环境科学
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食品科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2020
·
IF 9.038
Linfeng Chen,Xike Tian,Yong Li,Liqiang Lu,Yulun Nie,Yanxin Wang
Accurate screening of organophosphorus and carbamates pesticides from the complex real sample is crucial for water quality analysis and food safety control. Herein, a simple, low-cost and accurate pesticides screening method based on a high-throughput optical array system assembled by multiple cholinesterases (ChE) and thiocholine (TCh) sensors is described. The detection mechanism is that the inhibition of ChE activity by pesticides reduces the TCh produced by the hydrolysis of butyryl/acetylthiocholine iodide, thus changing the fluorescence intensity of TCh sensor. The diverse response of ChEs to pesticides and different affinity of sensors to TCh ensure the high-throughput and distinguishable signal output, which allow the establishment of high discrete pesticide database with intra-cluster agglomeration and inter-cluster dispersion. By using the database, the screening of unknown real contaminated samples were successfully operated, and the screened pesticide species and concentrations were consistent with high-performance liquid chromatography. This screening strategy demonstrates the feasibility of replacing existing complex mass spectrometry-based screening strategy with simple optical analysis, providing a new idea for the development of simple accurate screening technologies for widespread organic pollutants including pesticides.
研究领域:
分析化学
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环境科学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
ANALYST
·
2018
·Vol. 143
·期 12
·2883-2888
·
IF 3.864
Hui Ouyang,Wenwen Wang,Qi Shu,Zhifeng Fu
A novel immunochromatographic assay (ICA) using a dual-readout signal probe was developed for multiplexed detection of pesticide residues by adopting methyl parathion and fenpropathrin as model analytes. Luminol-reduced Au nanoparticles (LRAuNPs) were synthesized and utilized in the proposed ICA platform as a colorimetric/chemiluminescent (CL) dual-readout probe. The methyl parathion antibody and fenpropathrin antibody were tagged with the prepared LRAuNPs to conduct spatially-resolved multiplexed detection. After the occurrence of two immunoreactions on the test strip, the probes were captured by the immobilized antigens on the two test zones. The red color resulting from the accumulation of captured LRAuNPs was adopted as the visual and semi-quantitative readout. For the sensitive quantitative detection of the analytes, the CL signals caused by the luminophore in the LRAuNPs were collected after triggering the luminol-H2O2 CL reaction. Under the optimal conditions, the detection limits for methyl parathion and fenpropathrin were 0.17 ng mL−1 and 0.10 ng mL−1 (S/N = 3), respectively. The whole procedure for ICA was completed within 15 min. The present ICA protocol was successfully applied for detection of pesticide residues in spiked traditional Chinese medicine samples. This dual-readout ICA platform showed merits such as low cost, time efficiency, easy operation and high sensitivity. Its application potential has been demonstrated in the rapid screening and field detection of multiple pesticide residues.
研究领域:
分析化学
·
环境科学
·
食品安全
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2023
·
IF 12.6
Wanqi Jiang,Zan Yang,Fei Tong,Siyu Zhang,Lu Zhu,Lei Wang,Lunjing Huang,Kang Liu,Mingming Zheng,Yibin Zhou,Ruyan Hou,Yingnan Liu
In this study, based on the oxidase activity and photothermal effect of manganese dioxide nanosheets (MnO 2 NSs), with thiamine (TH) as the fluorescence response signal and tris (2,2'-bipyridyl) ruthenium (II) hexahydrate as the reference signal, an enzyme-regulated ratiometric fluorescence and photothermal dual-mode probe was constructed for the quantitative detection of organophosphorus pesticide (OPs) residues. OPs reduced the production of the reductive product thiocholine by inhibiting the activity of acetylcholinesterase , thereby regulating the residual amount of MnO 2 NSs. With the increase of OPs concentration, the color of the probe solution gradually transitioned from red to blue, and the temperature gradually increased. Using dichlorvos and chlorpyrifos as pesticide models, the developed probes exhibited sensitive responses to OPs in a wide linear range of 0.1–8000 ng/mL. The detection limits of dichlorvos and chlorpyrifos in fluorescence mode were 1.13 × 10 −3 ng/mL and 0.86 ng/mL, respectively. The corresponding detection limits in photothermal mode were 1.01 ng/mL and 1.02 ng/mL, respectively. The proposed probe displayed excellent anti-interference and reliability in the analysis of OPs residues in real samples. The dual-mode probe with self-verification function is expected to provide more accurate and robust detection results than the single-mode probe, and has a wider application prospect.
研究领域:
分析化学
·
环境科学
·
材料科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）