Foods
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2023
·Vol. 13
·期 1
·31
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IF 5.2
Zongjie Li,Yinyin Jin,Xingyu Wang,Liudong Xu,Liyan Teng,Kang Fu,Baoling Li,Yulu Li,Ying Huang,Ning Ma,Feng Cui,Tingting Chai
Intensive aquaculture combatting the decline of large yellow croaker populations can trigger bacterial outbreaks, resulting in extensive antibiotic use. In this study, we screened 5 aquaculture sites in the coastal areas of Zhejiang and identified 17 antibiotics in large yellow croakers using UPLC-MS/MS. The distribution and occurrence of antibiotic pollutants were different in the different tissues of large yellow croakers, being primarily dominated by quinolones. Relatively higher average residue levels of enrofloxacin and ciprofloxacin were detected in the inedible parts, specifically the gills (37.29 μg/kg). Meanwhile, relatively high average residue levels of enrofloxacin and ciprofloxacin were also found in the edible parts, particularly in the muscle (23.18 μg/kg). We observed that the residue levels detected in the swim bladder exceeded the prescribed limit for fish muscle, but there is currently no specific regulatory limit established for this particular tissue. Despite the HI values of enrofloxacin and ciprofloxacin being below 0.01, the health risks should not be disregarded. The findings of this research provide significant practical implications for assessing antibiotic contamination and enhancing the risk management of coastal regions.Keywords:quinolones;large yellow croaker;edible parts;health risk
研究领域:
水产养殖
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环境健康
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食品安全
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Bohan Wang,Lanhua Liu,Haopeng Zhang,Zhiqiang Wang,Kang Chen,Bo Wu,Limin Hu,Xiaohong Zhou,Lanlan Liu
Background Quinolones (QNs) widely exist in the environment due to their wide range of applications and poor metabolic properties, resulting in the generation and spread of resistance genes, posing a potential threat to human health. Traditional analytical methods cannot detect all broad ranges of QNs simultaneously. The development of facile, efficient and reliable method for quantification and assessment of the total QNs is a long-lasting challenge. Results We hereby provide a simple, sensitive and instantaneous group-targeting biosensor for the detection of total QNs in environmental water samples. The biosensor is based on a group-specific antibodies with high affinity against QNs. Fluorescent labeled antibodies bound to the coated antigen modified on the surface of the transducer, and excited by the evanescent waves. The detected fluorescent signal is inversely proportional to the QNs concentration. This biosensor exhibited excellent performance with detection limits lower than 0.15 μg L −1 for all five QNs variants, and even lower than 0.075 μg L −1 for ciprofloxacin (CIP) and ofloxacin (OFL). Environmental water samples can be detected after simple pretreatment, and all detection steps can be completed in 10 min. The transducer has a high regenerative capacity and shows no significant signal degradation after two hundred detection cycles. The recoveries of QNs in a variety of wastewater range from 105 to 119%, confirming its application potential in the measurement of total QNs in reality. Significance The biosensor can realize rapid and sensitive detection of total QNs in water samples by simple pretreatment, which overcomes the disadvantage of the traditional methods that require complex pretreatment and time-consuming, and pave the groundwork for expansive development centered around this technology.
研究领域:
环境科学
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分析化学
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生物技术
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DOI
PubMed
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Mengyue Wang,Yu Wang,Nanjiong Pang,Mingyao Wang,Yunxiang He,Xiaoling Wang,Junling Guo
Ubiquitous antibiotics threaten human health and ecosystem sustainability, and existing removal strategies, especially conventional multistep water treatments, are primarily limited by the antibiotic-specific removal capability. Here, we explore the natural biomass, plant polyphenols, in the capture of various antibiotics with a facile treatment─polyphenol-mediated antibiotic-independent supramolecular coagulation (PMAC). The PMAC shows a superior performance in removing five tetracyclines and quinolones (up to 98.54%), even under complex environmental parameters, including different pH, the presence of inorganic particles and ionic strength, and the presence of conventional colloid-associated contaminants. Our mechanistic studies suggested that PMAC is capable of exerting multiple molecular interactions with various antibiotics, and the coordination-driven self-assembly further destabilizes the phenolic–antibiotic nanocomplexes, enabling an antibiotic-independent coagulation. Collectively, the combination of efficient remediation with inexpensive biomass suggests a simple and scalable method for the sustainable removal of antibiotics. Our strategy shows great promise as a cost-effective, facile approach to eliminate antibiotics capable of being integrated into the currently existing water treatment systems.
研究领域:
环境科学
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生物化学
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DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2021
·
IF 7.963
Yin-Zhi Lv,Xiao-Jun Luo,Jian-Liang Zhao,Shan-Quan Wang,Bi-Xian Mai
Antibiotic levels in black-odor water could reflect the usage amount of antibiotics in population. On the other hand, these antibiotics are the source of antibiotics in the environmental water. Currently, researches on antibiotics in black-odor sediments are still lacking. In this study, 174 black and odor sediment samples from 74 cities in 28 provinces in China were collected for analysis. Among 44 targeted antibiotics, 13 antibiotics were detected in more than 30% of sediment samples. Fluoroquinolones and tetracyclines were the predominant antibiotics in these field samples, with average concentrations of up to 2074 and 1902 ng/g dry weight (dw), respectively, followed by macrolides (87.9 ng/g dw), lincosamides (8.06 ng/g dw) and sulfonamides (8.38 ng/g dw). High antibiotic contamination levels were almost always detected in black-odor sludges from economically less developed small cities; however, the difference in antibiotic concentrations between well-developed and small cities in China was not statistically significant. In addition, among the seven regions within China, no significant difference in concentrations was observed for the most antibiotics. Variances in antibiotic composition patterns in the 28 provinces of China may be due to differences in bacterial resistance, prescription habits, efficacy, and sediment carbon concentrations among various regions. There were significant positive correlations among some antibiotics in the same or different classes.
研究领域:
环境科学
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公共卫生
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药理学
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DOI
高影响因子
Journal of Environmental Sciences
·
2025
·
IF 6.3
Bo Gong,Chang Xu,Shan Zhao,Xiao-Min Sun,Shu-Guang Wang,Xian-Zheng Yuan,Chao Song
The photochemical transformation of fluoroquinolones (FQs) represents a crucial environmental phenomenon eliciting growing concern. Nevertheless, the impact and mechanisms of ubiquitously coexisting copper ions, i.e., Cu(II), remain elusive. Here, the role of Cu(II) in the photochemical transformation of FQs was systematically investigated via experimental characterizations and theoretical simulations. Cu(II) markedly inhibited the photolysis of levofloxacin (LEV), a representative FQs model, from 0.0078 to 0.0040 min −1 after a 100-min irradiation under simulated sunlight with the molar ratio of Cu(II): LEV at 1:1. The micro-level mechanisms were explored, including the ligand-to-metal charge transfer in complexation, modifications to reactive sites and activities with various reactive species, and higher hole-electron separation extents. In addition, the triplet excited state, singlet oxygen, and hydroxyl radical in LEV and LEV-Cu(II) with 1: 1 molar ratio played pivotal roles in the transformation, contributing 20.77/20.02 %, 13.87/14.59 %, and 7.96/4.68 %, respectively. Furthermore, considering more FQs models, the inhibition of Cu(II) on the transformation was found to universally exist in ciprofloxacin, enoxacin, enrofloxacin, norfloxacin, ofloxacin and pefloxacin, and their multiple molecular descriptors were changed with a same tendency. This work provides a comprehensive understanding of the transformation of antibiotics within complex aqueous matrices, contributing to further estimation of antibiotics removal and ecological risks.
研究领域:
环境科学
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药物化学
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生物无机化学
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DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2019
·Vol. 26
·期 33
·34345-34356
·
IF 2.914
Liu Lili,Chen Xin,Wang Zhiping,Lin Sen
Fluoroquinolones (FQs) occur broadly in natural media due to its extensive use, and it has systematic effects on our ecosystem and human immunity. In this study, long-root Eichhornia crassipes was reclaimed as a multi-functional activated carbon (MFAC) to remove fluoroquinolones (FQs) from contaminated water. To get insight into the adsorption mechanism, multiple measurements, including FTIR and XPS analyses, were employed to investigate the adsorption processes of ciprofloxacin and norfloxacin as well as the experiments of effect of exogenous factors on adsorption performances. The results confirmed that the adsorption of FQs by MFAC was mainly attributed to the electrostatic interaction, hydrogen bond interaction, and electronic-donor-acceptor (EDA) interaction. In addition, the kinetics and thermodynamics experiments demonstrated that the MFAC possessed great adsorption performance for FQs. According to the Langmuir model, the saturated adsorption capacities exceeded 145.0 mg/g and 135.1 mg/g for CIP and NOR at 303.15 K, respectively. The column experiments were conducted to explore the application performance of MFAC on the advanced treatment of synthetic water at different flow rates and bed depths. The adsorption capacity of CIP on MFAC was estimated by the Thomas models and the bed-depth service time (BDST) models, reaching 127.56 mg/g and 11,999.52 mg/L, respectively. These results also provide a valid approach for the resource recycling of the redundant long-root Eichhornia crassipes plants.
Graphical abstract
研究领域:
环境科学
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材料科学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2025
·
IF 5.1
Su Han Wang,Min Lin,Jing Liu,Jian Ping Wang,Ning Peng Wu
The quinolone efflux pump (QepA) protein of a fluoroquinolones-resistant Escherichia coli was grafted on Fe 3 O 4 to synthesize a type of magnetic composite Fe 3 O 4 @QepA. This material was combined with 4 fluorescent tracers to develop a semi-homogeneous fluorescent array on 96-well microplate for determination of fluoroquinolones (FQs) in chicken, and the results were analyzed by using linear discriminant analysis method. Results showed this method could discriminatively determine 15 FQs at the concentration ≥10 ng/g, and could identify each drug species at the concentration ≥2 ng/g. The method exhibited satisfactory recoveries in chicken sample (61.7 %–119.8 %). The analysis results for some unknown samples were consistent with a liquid chromatography mass spectrometry method. This is the first paper reporting a semi-homogeneous fluorescent array for discriminative determination of small molecule substances, and this array can be used as an accurate tool to determine the 15 FQs in meat sample.
研究领域:
分析化学
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微生物学
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食品安全
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2015
·
IF 2.729
Huili Wang,Ming Gao,Mei Wang,Rongbo Zhang,Wenwei Wang,Randy A. Dahlgren,Xuedong Wang
Herein, we developed a novel integrated device to perform phase separation based on ultrasound-assisted salt-induced liquid–liquid microextraction for determination of five fluoroquinones (FQs) in human body fluids. The integrated device consisted of three simple HDPE components used to separate the extraction solvent from the aqueous phase prior to retrieving the extractant. A series of extraction parameters were optimized using the response surface method based on central composite design. Optimal conditions consisted of 945 μL acetone extraction solvent, pH 2.1, 4.1 min stir time, 5.9 g Na 2 SO 4 , and 4.0 min centrifugation . Under optimized conditions, the limits of detection (at S/N = 3) were 0.12–0.66 μg L −1 , the linear range was 0.5–500 μg L −1 and recoveries were 92.6–110.9% for the five FQs extracted from plasma and urine. The proposed method has several advantages, such as easy construction from inexpensive materials, high extraction efficiency, short extraction time, and compatibility with HPLC analysis. Thus, this method shows excellent prospects for sample pretreatment and analysis of FQs in human body fluids.
研究领域:
分析化学
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药物分析
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医学检测
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DOI
PubMed
高影响因子
AQUATIC TOXICOLOGY
·
2023
·
IF 4.5
Dantong Jia,Xiuqi You,Moran Tang,Yitao Lyu,Jingrun Hu,Weiling Sun
Fluoroquinolones (FQs) are known to have genotoxicity to aquatic organisms. However, their genotoxicity mechanisms, individually and in combination with heavy metals, are poorly understood. Here, we investigated the single and joint genotoxicity of FQs, ciprofloxacin (CIP) and enrofloxacin (ENR), and metals (Cd and Cu) at environmentally relevant concentrations (0.2 µM) to zebrafish embryos. We found that FQs or/and metals induced genotoxicity (i.e., DNA damage and cell apoptosis) to zebrafish embryos. Compared with their single exposure, the combined exposure of FQs and metals elicited less ROS overproduction but higher genotoxicity, suggesting other toxicity mechanisms may also act in addition to oxidation stress. The upregulation of nucleic acid metabolites and the dysregulation of proteins confirmed the occurrence of DNA damage and apoptosis, and further revealed the inhibition of DNA repair by Cd and binding of DNA or DNA topoisomerase by FQs. This study deepens the knowledge on the responses of zebrafish embryos to exposure of multiple pollutants, and highlights the genotoxicity of FQs and heavy metals to aquatic organisms.
研究领域:
环境科学
·
毒理学
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DOI
PubMed
高影响因子
APPLIED CLAY SCIENCE
·
2022
·
IF 5.907
Qianqian Chang,Boqiang Gao,Hu Yang
In this work, a series of novel composite adsorbents (k-TAFe) was designed and fabricated through a one-step method, that is, the chelation crosslinking of tannic acid, kaolin particles and different contents of Fe(III). k-TAFe had a high efficiency in adsorption of various fluoroquinolones antibiotics (FQs) from water. The detailed performance of k-TAFe in FQs adsorption, including the influences of Fe(III) content, solution pH, inorganic and organic additives, adsorption time and FQs' concentration, has been investigated systematically using ofloxacin (OFL), one of popular FQs, as an example. The maximal OFL uptake of k-TAFe was 0.405 mmol/g at an initial pH of 6.0. On the basis of the analysis of the x-ray photoelectron and fourier transform-infrared spectra before and after adsorption, the comparison in the apparent performance of k-TAFe in adsorption of seven FQs with similar structures and their two sub-structural analogs, and theoretical calculation, multiple adsorption mechanisms, including chelating effect, hydrogen bonding, electrostatic attraction , and π-π electron–donor–acceptor interactions, were involved and discussed in detail. Among them, chelating effect due to Fe(III) played a key role in OFL adsorption, and the addition of kaolin particles synergistically enhanced Fe(III) fixed on the composite adsorbents, increased the Fe(III) contents, and caused an improved OFL uptake of k-TAFe. Because of the evident advantages of environmental-friendliness, high adsorption performance and low cost, k-TAFe as an efficient adsorbent has notable application potentials in removal of FQs from water.
研究领域:
环境科学
·
材料科学
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DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Hongmei Liu,Yue Li,Sikai Wang,Sisi Zhang,Guoqi Zhang,Yan Zhao
Herein, a new, environmentally friendly, and economical magnetic solid-phase extraction method for fluoroquinolones (FQs) from milk samples was developed using novel recyclable zeolitic imidazolate framework functionalized magnetic multiwalled carbon nanotubes (Fe 3 O 4 @MWCNTs@SiO 2 @ZIF-8) as adsorbents. Various characterization techniques, including scanning electron microscopy, N 2 adsorption-desorption analysis, and vibrating sample magnetometry, demonstrated that the adsorbent possessed a remarkable specific surface area, pore volume, and superparamagnetic properties, rendering it an excellent adsorbent. Combined with high-performance liquid chromatography, this method exhibited excellent linearity (R 2 ≥ 0.9991) over the concentration range of 0.5–500 μg L −1 , low limits of detection (0.10–0.34 μg kg −1 ), and low limits of quantification (0.30–1.00 μg kg −1 ). Finally, the developed method was successfully applied to analyze FQs in milk samples with recoveries ranging from 83.3% to 107.7% and relative standard deviations below 4.2%. The high efficiency and sensitivity of this method highlight the potential application of Fe 3 O 4 @MWCNTs@SiO 2 @ZIF-8 for analyzing FQs in complex matrices.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2020
·
IF 3.99
Chun Yang,Rui Li,Qi Wang,Wenhao Wang,Pei Gao,Bibo Hu
The functionalized mesoporous silica (FMS) and core-shell magnetic functionalized mesoporous silica (MMS) were synthesized on the basis of unfunctionalized mesoporous silica (UMS) via alkyl modification and one-pot reaction. Its adsorption properties to enrofloxacin (ENR), pefloxacin (PEF) and ciprofloxacin (CIP) were systematically investigated in single and binary systems. The results indicated the adsorption performance of MMS is five times higher than UMS. Batch adsorption experiments demonstrated the MMS had a higher adsorption capacity and adsorption efficiency for FQs, with the maximum adsorption capacities of 201.52 mg g -1 for CIP, 275.46 mg g -1 for PEF and 286.35 mg g -1 for ENR. Meanwhile, over 80% and 90% removals were achieved within 5 min and 10 min. Experiment results also presented that: MMS was able to reach the maximum adsorption capacity around pH 7.0 and maintain high adsorption capacity at a high concentration of humic acid. Besides, MMS showed the selective adsorption to three FQs in the binary adsorption experiments. The reusability experiment suggested the MMS possess excellent stability. H-bonding, electrostatic interaction and hydrophobic interaction were the main driving force between three FQs and MMS. Moreover, the adsorbent can be readily separated from water solution by an external magnet. These obtained results could be used for adsorbent design in considering of the properties of the adsorbates and the reuse of adsorbents.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）