PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
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2024
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IF 4.2
Xuan Zhao,Mengsong Chen,Hanxin Mao,Chuanqing Zhang,Jianyan Wu
Gray mold caused by Botrytis spp. is a common disease on various hosts, including strawberries. In this study, we obtained 59 Botrytis isolates from strawberries from greenhouses in Zhejiang Province, China. Identification of the sampled isolates at species level was performed by a duplex PCR assay method, the result showed that, in Zhejiang, gray mold on strawberry fruits is caused by a complex of Botrytis groups including B. cinerea group N (47.5 %) and B. cinerea group S (52.5 %). The sensitivities of all Botrytis isolates to pyrimethanil were determined based on discriminatory dose method on L-asparagine-based agar medium plate. Our results showed that the isolates obtained from the greenhouses with continuous use of pyrimethanil developed severe resistance to pyrimethanil, and the resistance frequencies of B. cinerea group N and group S isolates were 89.3 % and 41.9 %, respectively. By sequencing, four different resistance-related point mutations were identified in 38 Botrytis isolates: Bcpos 5 L412F (16 isolates, 42.1 %), Bcpos 5 L412V (14 isolates, 36.8 %), Bcmdl 1 E407K (2 isolates, 5.3 %), and Bcpos 5 L412S ⸱ Bcmdl 1 E407K (1 isolate, 2.6 %). The exogenous addition of methionine could not completely alter the resistance of Botrytis isolates to pyrimethanil. In this study, the pyrimethanil resistance in Botrytis isolates was steadily inherited, and compared to the pyrimethanil-sensitive isolates, the resistant mutants exhibited good fitness in sporulation capacity, spore germination rate, and virulence on strawberries. In conclusion, our results provided a description of the genetic structure of Botrytis groups complex on strawberry fruits and reminded growers to focus on the stable pyrimethanil resistance in Botrytis isolates, caused by point mutations in BcPos5 and BcMdl1.
研究领域:
植物病理学
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分子遗传学
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高影响因子
ANALYTICA CHIMICA ACTA
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2025
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IF 6
Lijuan Lin,Peng Gao,Minjin Wu,Kui Luo,Jianping Li
Background Molecularly imprinted polymer (MIP) sensors have been used to detect trace amounts of pesticide residues. But the difficulty of inert analytes in producing measurable signals often limits the application of MIP sensors. The inherent limitations of the signal transduction mechanism impose fundamental constraints on the enhancement of detection sensitivity. Results Herein, a strategy was proposed for sensitive, selective and rapid determination of trace pyrimethanil (PMT) residues using MIP sensors by the aid of dummy template imprinting. MIP film was synthesized using vinyl pyrimethanil (Abbr. PMT-Vi), a structural analogue of PMT, as the dummy template molecule. After the formation of MIP film, analyte PMT was added for competing with PMT-Vi in the imprinted cavities of MIP. Then, Fe/N-PAMAM-SH, a dendritic polymer bound with Fe/N CDs, was immobilized onto the MIP via the Thiol-ene click reaction between Fe/N-PAMAM-SH and PMT-Vi. PMT was indirectly determined by measuring the ECL signal of Fe/N CDs in the luminol-hydrogen peroxide system. Significance The strategy greatly enhances the detection sensitivity of MIP sensors, achieving a detection limit of 2.58 × 10 -16 mol/L. It also offers a novel approach for detecting molecules that lack characteristic signals or generate weak signals.
研究领域:
分析化学
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农药残留检测
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生物传感技术
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ANALYTICAL CHEMISTRY
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2025
·
IF 6.7
Haowei Dong,Pengwei Zhang,Haifang Wang,Jingcheng Huang,Zhen Guo,Ziping Cai,Ibrahim A. Darwish,Yemin Guo,Xia Sun
As a simple and visually assessable point-of-care testing method, lateral flow assays (LFAs) are widely used for detecting procymidone in vegetable samples. Meanwhile, an increasing number of aptamer-based LFAs are being developed for rapid detection. However, the sensitivity of conventional gold nanoparticles (AuNPs) aptamer-based LFAs is limited, which is a challenge to meet the detection requirements. This study proposed a dual amplification strategy of click chemistry and hybridization chain reaction (HCR) to construct a colorimetric probe to enhance the sensitivity. The nucleic acid nanostructure (HCR-ALK) constructed by HCR was used as a programming template to guide the click chemical reaction to induce the cross-linking of AuNPs-N3 and HCR-ALK to form a uniform and stable ″ballon-string″ colorimetric probe (HCR-AuNPs). This strategy achieved dual-signal amplification. Under optimal conditions, the limit of detection (LOD) of this LFA was 5.4 × 10–2 ng/mL. Compared with the conventional AuNPs aptamer-based LFAs, its LOD had been improved by 13.69-fold, and its color display time had been reduced to one-tenth. In specificity and stability experiments, this LFA showed a satisfactory performance. Moreover, it was successfully applied to the detection of procymidone in vegetable samples, with a recovery rate maintained between 93.44%–104.00%. Thus, this study provides a signal amplification strategy to address the issue of insufficient sensitivity in aptamer-based LFAs. This will offer new insights for the development of aptamer-based LFAs.
研究领域:
生物化学
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分子诊断
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食品科学
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JOURNAL OF HAZARDOUS MATERIALS
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2025
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IF 11.3
Wenying Ding,Bingfeng Chen,Minglong Song,Meng Liu,Binghai Lv,Danyan Qiu,Yuke Zhu,Zhenyan Zhang,Mengwei Zhang,Rongshe Zhang,Tao Lu,Haifeng Qian
Heterocyclic compounds are extensively used in pharmaceuticals and agrochemicals, yet their persistence and bioavailability in soil may disrupt microbial functions and ecosystem health. To address these impacts, we performed a metagenomic sequencing to assess the impact of three such compounds--cefapirin, pyrimethanil, and quinclorac on soil microbial communities at 15 and 30 d exposure. Our results revealed distinct compound-specific and time-dependent effects. Cefapirin initially induced minimal changes at 15 days but significantly reduced eukaryotic diversity and functional potential by 30 days, while also enriching virulence factors. Pyrimethanil strongly perturbed the community at 15 days, suppressing metabolic pathways and elevating the abundance of antibiotic resistance genes (ARGs) and virulence factors, along with consistently enriching mobile genetic elements (MGEs) associated with these genes—though some effects diminished by 30 days. Quinclorac exerted comparatively milder inducing subtle shifts in virulence factor profiles and exerting limited influence on antibiotic resistance gene abundance. Spearman correlation analysis linked compound-induced shifts in dominant microbial phyla (notably Pseudomonadota and Actinomycetota) to the dynamics of ARGs and virulence factors. These results underscore that the ecological risks of heterocyclic compounds depend critically on both compound properties and exposure duration. Our findings provide valuable insights for guiding risk assessment and sustainable practices to mitigate the ecological risks of agrochemicals.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2020
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IF 3.232
Zhengdong Shen,Qinzhen Fan,Qian Yu,Rui Wang,Huan Wang,Xianming Kong
This work aims to isolate and detect pesticide (carbendazim) residue in real food samples: orange juice and kale leaves. The combination of on-chip thin layer chromatography (TLC) and surface enhanced Raman scattering (SERS) spectroscopy was used for the separating and detecting of carbendazim (MBC) from the complex food sample. In order to achieve on-site detection of MBC from real food sample, the portable Raman spectrometer was coupled with TLC-SERS. The porous stationary phase composed of diatomite biosilica is beneficial for SERS enhancement and eluent migration. The experiments exhibited that the diatomite chip was suitable for TLC separation and has not shown SERS background and provided excellent separation efficiency, 10 −8 M silver colloids were appropriate for the SERS measurement on TLC chip. The food sample was directly spotted onto the diatomite chip for TLC separation without any pretreatment. The separation and detection process were finished in less than 5 min, the mixture of pyrimethanil , pymetrozine and MBC could be distinguished simultaneously by TLC-SERS at one diatomite chip. The MBC in orange juice and kale were successfully detected, and a limit of detection (LOD) less than 2 ppm could be achieved.
研究领域:
分析化学
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食品安全
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光谱学
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TALANTA
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2018
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IF 4.244
Kaiqiang Wang,Da-Wen Sun,Hongbin Pu,Qingyi Wei
The residual of pesticides in fruit and vegetables is one of the major food safety concerns for consumers. There is a demand for easy and rapid analytical methods to sense pesticide residues in foods. In this study, a core-shell Au@Ag nanoparticles aggregates (Au@AgNAs) based surface-enhanced Raman scattering (SERS) method was developed to detect trace amount of difenoconazole . Results suggested that by targeting the characteristic peaks at 700 and 808 cm −1 , the logarithmic SERS signal intensities and logarithmic difenoconazole concentrations in the range from 5 × 10 −7 to 2.5 × 10 −5 M showed linear relationship with the coefficient of determination ( R 2 ) of 0.990 and 0.985, and limit of detection (LOD) values of 5.01 × 10 −8 and 2.8 × 10 −8 M, respectively. The Quick Easy Cheap Effective Rugged and Safe (QuEChERS) sample preparation method was used to extract difenoconazole in grapes for SERS measurements. The LOD of difenoconazole in grapes using this developed method was as low as 48 μg/kg, which was significantly lower than the maximum residue limit (MRL) values prescribed by European Union and China. This study demonstrated that the Au@AgNAs-based SERS method can be used as a simple, rapid and sensitive approach for sensing trace contaminants in food.
研究领域:
食品安全
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分析化学
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2018
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IF 3.412
Jiale Yang,Chen Fan,Gang Tang,Wenbing Zhang,Hongqiang Dong,You Liang,Yanfei Wang,Mingqiang Zou,Yongsong Cao
To elucidate the relationship between the structure of ionic liquid (IL) and its enrichment ability to trace pesticides from an environmental water sample, a series of imidazole-based ILs were synthesized to extract four fungicides (boscalid, cyprodinil, fluazinam, and pyrimethanil) through an in situ ionic liquid dispersive liquid–liquid microextraction method. The results showed that aromatic heterocyclic monocation ionic liquids (MILs) had better extraction ability to fungicides than other three alicyclic heterocyclic MILs. Dication ionic liquids (DILs) with the four carbons at the side chain had better ability to extract fungicides than MILs, and DILs with a long bridge carbon chain had better recoveries of fungicides with low Kow values. The proposed method showed high mean enrichment factors and high recoveries of the fungicides from real water samples. The rules of the relationship between the structure of IL and enrichment ability are instructive to the application of ILs in pretreatment of complex substances.
研究领域:
环境科学
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分析化学
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JOURNAL OF SEPARATION SCIENCE
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2019
·Vol. 42
·期 16
·2679-2686
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IF 2.516
Weixuan Yao,Zhefeng Fan,Suling Zhang
A zirconium terephthalate metal-organic framework-incorporated poly( N -vinylcarbazole- co -divinylbenzene) monolith was fabricated in a capillary by a thermal polymerization method. The optimized monolith had a homogeneous structure, good permeability, and stability. The monolith could be used for the effective enrichment of fungicides through π-π interactions, electrostatic forces, and hydrogen bonds. The potential factors that affect the extraction efficiency, including ionic strength, solution pH, sample volume, and eluent volume, were investigated in detail. The monolith-based in-tube solid-phase microextraction coupled with ultra-high-performance liquid chromatography and high-resolution Orbitrap mass spectrometry was performed for the analysis of five fungicides (pyrimethanil, tebuconazole, hexaconazole, diniconazole, and flutriafol) in environmental samples. Under the optimized conditions, the linear ranges were 0.005–5 ng/mL for pyrimethanil, 0.01–5 ng/mL for flutriafol, and 0.05–5 ng/mL for other fungicides, respectively, with coefficients of determination ≥0.9911. The limits of detection were 1.34–14.8 ng/L. The columns showed good repeatability (relative standard deviations ≤9.3%, n = 5) and desirable column-to-column reproducibility (relative standard deviations 5.3–9.4%, n = 5). The proposed method was successfully applied for the simultaneous detection of five fungicides in water and soil samples, with recoveries of 90.4–97.5 and 84.0–95.3%, respectively.
研究领域:
分析化学
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环境科学
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JOURNAL OF SEPARATION SCIENCE
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2021
·Vol. 44
·期 10
·2113-2120
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IF 3.645
Suling Zhang,Ziluo Hua,Hongting Zhao,Weixuan Yao,Yuanzhao Wu,Defeng Fu,Jiancong Sun
In this work, defective Zr-based metal–organic framework was successfully synthesized and evaluated as a dispersive micro-solid-phase extraction sorbent for efficient preconcentration and determination of fungicides in complex water samples. The defective Zr-based metal–organic framework crystal with increased adsorption capacity was successfully synthesized by employing formic acid as the modulator. The extraction conditions, including the pH, extraction time, desorption solvent and desorption time, were comprehensively investigated. Under optimum conditions, it was found that dispersive micro-solid-phase extraction method, coupled with liquid chromatography/mass spectrometry, exhibited a good linear relationship with correlation coefficients greater than 0.9980. The relative standard deviations of inter-day and intra-day precisions ranged from 2.6 to 9.2% and the limits of detection ranged from 0.004 to 0.036 μg/L. These merits, combined with their satisfactory recoveries (>80%), suggested the great potential of defective Zr-based metal–organic framework as a new adsorbent for efficient extraction of trace fungicides. This method exhibits good application potential for the pretreatment of fungicides from environmental water samples.
研究领域:
分析化学
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环境科学
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材料科学
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高影响因子
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