IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
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2020
·Vol. 57
·期 1
·39-47
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IF 1.814
Wu Qinggui,Yang Honglin,Sun Yuxi,Hu Jinyao,Zou Lijuan
In vitro culture is employed as a means of producing plant materials from medicinal plants to meet the increasing demands of the market. Herein, we focused on an efficient protocol for organogenesis through two pathways in six explant types from Caryopteris terniflora Maxim ( C. terniflora ), a medicinal and ornamental plant. For direct organogenesis, the maximum number of shoots per internode explant (62.5) was achieved on Murashige and Skoog (MS) medium containing 3.0 mg L −1 thidiazuron (TDZ) and the maximum number of shoot tips per explant (45.5) was obtained by 1.5 mg L −1 TDZ, with 100% response. A protocol was also developed for indirect organogenesis from leaf, leaf-petiole, petiole, and root explants on MS basal medium containing a range of TDZ concentrations. Leaf-petiole explants induced more adventitious shoots (57.6 per explant) and leaves (31.4 per explant) than petioles (37.5 per explant) or roots (25.0 per explant), and the differentiation rate ranged from 87.2 to 100%. The regenerated shoots were elongated on MS medium containing 0.3 mg L −1 α-naphthaleneacetic acid (NAA) and 1.0 mg L −1 gibberellic acid (GA 3 ). The elongated shoots developed roots on ½ MS medium containing 0.3 mg L −1 NAA. Successful acclimatization of rooted plantlets in a greenhouse was achieved with 100% survival. Furthermore, the embryogenic-like structure was observed and the morphological characteristics of C. terniflora assessed at different stages of in vitro development. Our protocol offers, for the first time, an efficient, scalable propagation procedure for C. terniflora useful for large-scale cultivation for the purposes of conservation and genetic manipulation.
研究领域:
植物学
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药用植物研究
✓ 已验证
DOI
高影响因子
JOURNAL OF PLANT GROWTH REGULATION
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2024
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IF 3.9
Pang Jinhui,Xiong Yuping,Zeng Yujie,Chen Xiaohong,Li Jianrong,Zhang Xinhua,Li Yuan,Wu Kunlin,Zeng Songjun,da Silva Jaime A. Teixeira,Ma Guohua
Tetrastigma hemsleyanum is a perennial evergreen vine of the Vitaceae. The entire herb is used in traditional Chinese medicine as a broad-spectrum plant-based antibiotic, so it has high economic and social value. Wild T. hemsleyanum resources are scarce, so it has been declared an endangered and rare medicinal plant. Seed yield is low and vegetative propagation by cuttings results in limited plant production, so development of the T. hemsleyanum industry requires optimized propagation protocols and the development of new biotechnologies to proliferate this plant in commercial quantities. In this study, shoot organogenesis was successfully induced from leaves and petioles. Two plant growth regulators, 6-benzyladenine (BA) and thidiazuron, induced callus and adventitious shoots, but the ideal adventitious shoot induction medium was Murashige and Skoog (MS) medium containing 1.0 mg L −1 BA and 0.1 mg L −1 α-naphthaleneacetic acid (NAA). This resulted in a shoot proliferation coefficient (SPC) of 6.73 within 30 d at a light intensity of 100 µmol m −2 s −1 . When light intensity was increased from 50 to 200 µmol m −2 s −1 , SPC (7.35), chlorophyll a (Chl a ), Chl b , and total Chl ( a + b ) content increased. On MS medium containing 0.1–2.0 mg L −1 NAA or indole-3-butyric acid, 100% of adventitious shoots formed adventitious roots. Plantlets showed no obvious morphological variation, and their survival exceeded 98% on a substrate of peat and river sand ( v:v = 2:1). This study’s protocols allow for the mass production of adventitious shoots for conservation purposes, and potentially for the commercial propagation of T. hemsleyanum .
研究领域:
植物学
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药用植物学
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产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Zongpu Han,Yixiong Lin,Xinyu Guo,Jieyun Xu,Xiaomeng Gao,Ruihan Yang,Yuan Zhao,Mixiao Gui,Linjun Zhang,Yuanlong Guo,Zetao Chen
The strategy of re-organogenesis provides an optimal framework for restoring complex organ structures and functions in adult damage. While the focus has often been on restoring organogenesis stem cells, there is limited investigations of reverting the environmental niche to support this approach. The guiding principle of “Nature selects the fittest to survive” drives the intricate dynamic changes in cellular events within the niche environment, especially through immune surveillance. The extracellular matrix (ECM) serves as the “self-associated molecular patterns” of the niche, containing extensive data on cell-niche reaction data and acting as the active tuner of immune surveillance. In this study, hyaluronic acid (HA) is identified as a unique component of the ECM in cranial osteo-organogenesis. Mechanistically, HA activates the Cluster of Differentiation 44 (CD44)-Mitogen-Activated Protein Kinase (MAPK)/Extracellular Signal-Regulated Kinase (ERK)-Ets Variant 1/5 (ETV1/5)- Mannose Receptor C-Type 1 (MRC1) axis in macrophages, establishing a distinct immune surveillance during osteo-organogenesis. Furthermore, HA is utilized as a novel engineered material for an “Osteo-organogenesis niche”, restoring immune surveillance and synergistically regulating stem cells to achieve re-osteo-organogenesis in cranial defects of rats. Taken together, the study unveils a previously unknown strategy for leveraging re-organogenesis by utilizing “organogenesis niche” ECM engineered materials to manipulate immune surveillance, thereby comprehensively regulating stem cells and other tissue cells effectively for re-organogenesis.
研究领域:
再生医学
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免疫学
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组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Bing Zhang,Sainan Zhang,Shuaiqi Wang,Haoyu Dong,Yibo Dong,Shaohui Yang,Jiehua Wang
Microplastics are emerging contaminants in terrestrial ecosystems, but their phytotoxic effects on woody plants remain poorly understood. Here, we exposed in vitro ‑grown hybrid poplar 84 K ( Populus alba × Populus glandulosa ) to 3 µm polystyrene (PS) microplastics (50 or 200 mg·L −1 ). PS significantly inhibited adventitious rooting, de novo shoots regeneration, and photosynthetic performance in a concentration-dependent manner. Root fresh weight, plant height, shoot number, total chlorophyll, and Φ PSII declined by 11 %, 10 %, 57 %, 9 %, and 9 % at 50 mg·L⁻¹ PS, and by 43 %, 19 %, 78 %, 21 %, and 20 % at 200 mg·L⁻¹ . Mechanistically, PS induced excessive reactive oxygen species (ROS) accumulation, impaired primary cell wall regeneration, delayed shoot organogenesis, and disrupted secondary xylem formation. We further tested whether 50 mg·L⁻¹ multi-walled carbon nanotubes (MWCNTs, M 50 ) could alleviate PS-induced damage. Co-application of M 50 effectively mitigated oxidative stress, restored auxin–cytokinin balance, and reactivated key genes involved in meristem maintenance and xylem differentiation. Scanning electron microscope (SEM) analysis revealed that MWCNTs reduced PS adhesion to root by forming PS–MWCNTs composite structures. Overall, this study demonstrates that microplastics impair poplar micropropagation via oxidative and developmental disruption, while MWCNTs alleviate these effects via both biochemical regulation and physical buffering. Our findings advance the understanding of microplastic–nanomaterial interactions in woody plants and suggest that nanomaterials could serve as potential tools in clonal propagation and ecosystem restoration under microplastic stress, though their ecological risks warrant careful assessment.
研究领域:
植物科学
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环境毒理学
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纳米技术
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生物技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
·
2018
·Vol. 65
·期 3
·372-383
·
IF 0.816
Zhang Y.,Yong Y. B.,Wang Q.,Lu Y. M.
Lily is regarded as one kind of the most economically important cut flowers in China. In this paper, lily cultivar ‘Aladdin’ ( Lilium longiflorum × Lilium asiatic ) was chosen as experimental material for its distinct high propagation coefficient in nature and three main conclusions were achieved. Firstly, our observation results revealed that the noticeable propagation coefficient of ‘Aladdin’ depended on strong capacity to form many underground stem axillary bulbils positioned on underground node of plant after 70 days of cultivation and having 1 cm flower buds. Secondly, in terms of physiological changes, zeatin riboside (ZR) relative concentration, soluble sugar and starch content peak when and where such bulbils are forming. Finally, two KNOX family Lilium genes were sequenced completely and one BELL family gene was sequenced partly; besides, there might be an interaction between KNOX-2 and BELL protein to accomplish their functions in bulbils organogenesis according to qRT-PCR results. As far as we know, it is the first time to study the formation mechanism of lily underground stem axillary bulbils. We illustrated physiological and molecular profiles, as well as offered a new prospect to lily molecular breeding in the future.
研究领域:
植物学
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分子生物学
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植物生理学
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遗传学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）