ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2025
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IF 3.8
Guo Mengyue,Ke Tongwei,Zhang Huiru,Zhang Jing,Qin Jia’an,Ruan Haonan,Tian Jiao,He Chunjiao,Luo Yawen,Qin Xuhua,Luo Jiaoyang,Yang Meihua
Thiamethoxam is one of the top three neonicotinoids found in the environment and in food. The widespread use of thiamethoxam in medicinal and edible herbs threatens their safe use. Therefore, it is particularly important to develop a rapid and simple approach to detect thiamethoxam residues in herbal medicines. In this study, colloidal carbon-based immunochromatographic strip (CNP-ICS) and colloidal gold-based immunochromatographic test strip (GNP-ICS) methods were respectively developed for the determination of thiamethoxam . The cutoff value of CNP-ICS was 0.1 ng mL −1 , which is six times more sensitive than that of the GNP-ICS method (0.6 ng mL −1 ). The CNP-ICS method was applied to detect thiamethoxam in medicinal and edible Lonicerae Japonicae Flos (LJF) for the first time, with a limit of detection of 10 ng g −1 . The detection results of thiamethoxam in 26 batches of LJF samples by the CNP-ICS were consistent with those of the LC–MS/MS method. High residue levels (10.16–530.40 ng g −1 ) and high detection rates (69.23%) of thiamethoxam in LJF were observed. The CNP-ICS prepared in this study has the merits of low cost, simple preparation, non-toxicity, and high sensitivity, which can be utilized for rapid field screening of thiamethoxam residues in complex matrices such as herbal medicines. Graphical
研究领域:
植物学
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分析化学
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环境科学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2024
·
IF 8.5
Zhengtao Li,Haowei Dong,Haifang Wang,Donghan Li,Shuxian Zhou,Shengxi Zhai,Jingcheng Huang,Rui Xu,Wenping Zhao,Mohamed Bedair M. Ahmed,Gili Bisker,Xia Sun,Yemin Guo
The presence of multiple pesticide residues in vegetables has become an increasingly critical concern. Immunochromatography has garnered significant interest for its convenience, sensitivity and speed. However, conventional immunochromatography was typically limited to single-target detection, exhibiting restricted efficiency and high costs in on-site large-scale detection scenarios. Dual-channel time-resolved fluorescent immunochromatography test strip (TRFITS) was developed for the simultaneous rapid detection of imidacloprid (IMI) and tolfenpyrad (TOL) in 15 min. Utilizing the excellent optical properties of time-resolved fluorescent microspheres, this TRFITS enabled better detection sensitivity (0.22 ng/mL for IMI, 11.85 ng/mL for TOL), which was approximately 20 times more sensitive than that of conventional immunochromatography, under optimal conditions. In addition, the TRFITS successfully detected IMI and TOL in vegetables samples with acceptable accuracy. The ability to simultaneously quantify multiple pesticide residues using a single test strip reduces material consumption and lowers operational costs, offering a practical solution for rapid on-site pesticide screening.
研究领域:
分析化学
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环境科学
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农业科学
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DOI
PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2026
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IF 10.6
Conghui Shan,Chenxin Gong,Yingying Yu,Weixia Zhang,Difei Tong,Kaiwen Hu,Yan Gao,Yang Shu,Xunyi Zhang,Guangxu Liu,Wei Shi
Buprofezin (BPFN), an insecticide that inhibits arthropod chitin synthesis, is widely applied in agriculture and increasingly detected in aquatic environments. Given the evolutionary conservation of chitin biosynthesis pathways, its potential impacts on non-target crustaceans remain poorly understood. In this study, we investigated the effects of environmentally relevant BPFN concentrations on the exoskeletal properties of the whiteleg shrimp Litopenaeus vannamei . Nanoindentation revealed significant reductions in exoskeletal stiffness and hardness following BPFN exposure. Consistently, chitin and total calcium content decreased, accompanied by reduced mineral density and cuticular volume as shown by histology and micro-CT imaging. These structural impairments were closely associated with altered patterns of molt-related physiological and biochemical indicators, including changes in glycogen storage, chitinase activity, carbonic anhydrase activity, and Ca 2 + dynamics, and metabolites annotated to steroid-related pathways. Integrated transcriptomic and metabolomic analyses further revealed that BPFN markedly suppressed amino sugar and nucleotide sugar metabolism, downregulating key enzymes required for chitin precursor generation. BPFN also interfered with ECM-receptor interactions and ER-associated protein processing, disrupting cuticular protein assembly, and perturbed TGF-β/BMP signaling pathways critical for calcium carbonate deposition. Correlation analyses linked these molecular disturbances with defects in chitin synthesis, matrix organization, and biomineralization. In summary, our findings demonstrate that BPFN exposure compromised the structural integrity and mechanical performance of L. vannamei exoskeleton by impairing chitin synthesis and recycling, disturbing cuticular protein processing, weakening calcium carbonate mineralization, and disrupting molt-associated physiological processes.
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PubMed
高影响因子
JOURNAL OF PEST SCIENCE
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2025
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IF 4.1
Lin Kai,Wu Hongxin,Li Zhongsheng,Zhong Zichun,He Liuyan,Guo Yujing,Zhang Jie,Xu Xiaoxia,Zhang Wenqing,Jin Fengliang,Pang Rui
Insecticide resistance in pest control poses a threat to agricultural production and human health. Numerous insect species express genes coding for detoxification enzymes that have broad substrate promiscuity thus conferring resistance to various insecticides. However, whether the homologs of these genes play similar roles in resistance phenotypes of closely related species remains largely unclear. Therefore, this study compares the resistance profiles of three major rice planthopper species (Delphacidae) ( Laodelphax striatellus , Nilaparvata lugens , and Sogatella furcifera ) based on the metabolic activity of their cytochrome P450s. Genome-wide analyses resulted in 68, 70, and 64 P450 genes in L. striatellus , N. lugens , and S. furcifera , respectively. Phylogenetic analyses among these genes found that most resistance-related genes in one species had homologs in other planthopper species. The most resistance-relevant orthogroup ( CYP6ER s) showed higher evolutionary instability than most other groups. RNAi and in vitro metabolism assays revealed that CYP6ER s confers more divergent insecticide resistance profiles among planthopper species than the other two major resistance-related P450 subfamilies ( CYP6AY s and CYP4C61 s). Alphafold-based structural predictions and alignments suggested that P450 orthogroups with higher phylogenetic instability tended to have less structural similarities, resulting in more divergent metabolic profiles. This relationship was also in silico validated on Aphidae aphids and Lepidoptera noctuids. This study proposes combined phylogenetic and toxicogenomic analyses for understanding CYPome-based insecticide resistance convergency and divergency among closely related pests. These findings may improve the accuracy and rationality of chemical pest control.
研究领域:
昆虫学
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毒理学
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基因组学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2025
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IF 7.7
Yilin Zhan,Zhongyong Xu,Lei Wang,Mingle Li,Xiaoqiang Chen,Taisheng Wang,Na Zhang,Bin Liu
The extensive agricultural use of chitin synthesis inhibitors (CSIs) has garnered significant concern due to their environmental persistence and potential ecological risks. A persistent challenge in this context is the concurrent discrimination and quantitative detection of multiple CSI residues in complex matrices. To address this issue, we developed a fluorescent sensor array utilizing a supramolecular system for the identification and quantification of four common CSIs: buprofezin, chlorfluazuron, diflubenzuron, and flufenoxuron. This system exploits the co-binding of a fluorescent indicator and CSI within the hydrophobic cavity of albumin, inducing pattern-based ratiometric fluorescence responses. The generated unique fluorescence fingerprint profiles for each CSI enable differentiation through multivariate statistical analyses, including linear discriminant analysis (LDA) and hierarchical cluster analysis (HCA), yielding 100 % classification accuracy across both individual CSIs and their mixtures. Furthermore, quantitative detection is facilitated by a linear correlation between LDA feature scores and CSI concentrations. The practical utility of this platform was substantiated by successful discrimination of CSIs in real-world samples, including millet, soil, and aqueous environments, with performance maintained across diverse matrix compositions, offering a critical advancement in addressing the analytical challenges posed by these persistent pesticide residues.
研究领域:
环境化学
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分析化学
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超分子化学
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农药检测
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荧光传感器
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高影响因子
ADVANCED FUNCTIONAL MATERIALS
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2020
·Vol. 31
·期 5
·2006606
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IF 16.836
Bin Liu,Yaxun Fan,Haofei Li,Weiwei Zhao,Siqi Luo,Hua Wang,Bo Guan,Qiaolian Li,Jiling Yue,Zhichao Dong,Yilin Wang,Lei Jiang
Vast wastage of pesticides has caused significant environmental pollution and economic loss, which occurs in any step during the entire process of pesticide application. However, the existing strategies for controlling pesticide losses are step specific. Here, a comprehensive strategy to substantively improve pesticide efficiency on the basis of precise designs from beginning to end is developed. A water-based coacervate with synthesized imine-based dynamic covalent trimeric surfactants to synergistically control encapsulation, deposition, retention, and release of pesticides on water-repellent plants is constructed. The coacervate consists of nanosized networks and abundant tightly bonded water, leading to effective encapsulation of hydrophilic/hydrophobic pesticides. Meanwhile, the network-like microstructure entangles with the micro/nanostructures of superhydrophobic surface, ensuring complete deposition on superhydrophobic plant surface after high-speed impact and inhibition of wind/rainwater erosion. Moreover, the CO 2 -induced degradative surfactant coacervate determines the precise pesticide release. The dynamic coacervate as an innovative pesticide formula provides a prospective way for pesticide application, and is expected to promote productive and sustainable agriculture.
研究领域:
农业科学
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材料科学
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环境科学
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高影响因子
CHROMATOGRAPHIA
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2013
·Vol. 76
·期 11
·671-678
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IF 1.437
Cui Shumin,Chen Qianxia,Wang Weiping,Miao Jigen,Wang Aijun,Chen Jianrong
In this study, a simple and efficient method has been developed to analyze pesticides in water samples using ultrasonic-assisted dispersive liquid–liquid microextraction (UA-DLLME) combined with gas chromatography-flame ionization detection (GC-FID). Several parameters, including type and volume of extractant and dispersant, extraction time, and amount of salt on extraction performance, were optimized in detail. A mixture of acetonitrile (1.0 mL, dispersant) and carbon tetrachloride (15 μL, extractant) was used for extraction. Under optimal conditions, enrichment factors were obtained between 315 and 1153. The linearity of the method ranged from 1 to 100 μg L −1 with correlation coefficients ≥0.9990. Limits of detection (S/N = 3) ranged between 0.09 and 0.57 μg L −1 , depending on the compounds. Relative standard deviations were <8.0 % ( n = 5) for both intra- and inter-day analyses. The proposed method was successfully applied for the preconcentration and determination of pesticides in water samples (river water, tap water, and lake water) with recoveries that varied from 90.5 to 107.7 %.
研究领域:
分析化学
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环境科学
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水质分析
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DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2017
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IF 3.981
Weiqian Liang,Juntao Wang,Xiaohuan Zang,Wenhuan Dong,Chun Wang,Zhi Wang
In this work, a barley husk biomaterial was successfully carbonized by hydrothermal method. The carbon had a high specific surface area and good stability. It was coated onto a stainless steel wire through sol-gel technique to prepare a solid-phase microextraction fiber for the extraction of trace levels of twelve pesticides (tsumacide, fenobucarb, indoxacarb, diethofencarb, thimet, terbufos, malathion, thiamethoxam, imidacloprid, buprofezin, acetamiprid, thiamethoxam) from vegetable samples prior to gas chromatography–mass spectrometric (GC–MS) detection. The main experimental parameters that could influence the extraction efficiency such as extraction time, extraction temperature, sample pH, sample salinity, stirring rate, desorption temperature and desorption time, were investigated. Under the optimized conditions, the linearity was observed in the range of 0.2–75.0 μg kg −1 for tomato samples, and 0.3–60.0 μg kg −1 for cucumber samples, with the correlation coefficients ( r ) ranging from 0.9959 to 0.9983. The limits of detection of the method were 0.01–0.05 μg kg −1 for tomato samples, and 0.03–0.10 μg kg −1 for cucumber samples. The recoveries of the analytes for the method from spiked samples were in the range of 76%–104%, and the precision, expressed as the relative standard deviations, was less than 12%.
研究领域:
分析化学
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环境科学
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DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 6
·期 10
·3388-3394
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IF 1.938
Weiping Wang,Hualing Zhu,Shumin Cui,Jigen Miao,Jianrong Chen
An ultrasound-assisted dispersive liquid–liquid microextraction method based on solidification of floating organic droplets (UA-DLLME-SFO) followed by gas chromatography-flame ionization detection (GC-FID) was developed for the extraction and determination of pesticides including buprofezin, triazophos, λ-cyhalothrin, pyridaben, and es-fenvalerate in water samples. The influence of different parameters affecting the performance (the nature of extractant and disperser solvents as well as their volumes, salt addition, extraction and ultrasonication time) was evaluated in detail. Under the optimized conditions (extraction solvent: 8 μL 1-dodecanol; dispersive solvent: 300 μL acetonitrile; 1% (w/v) NaCl; ultrasonication time: 3 min), the obtained enrichment factors were between 143 and 813. The linear range was 0.20–200 μg L−1 with correlation coefficients higher than 0.9976. Based on a signal-to-noise ratio of 3, limits of detection were in the range of 0.11 to 0.48 μg L−1, depending on the compounds. Relative standard deviations (%) were less than 3.5% (n = 5) for both intra- and inter-day analyses. The proposed UA-DLLME-SFO-GC-FID method was successfully applied for the determination of pesticides in water samples with recoveries varying from 92.2 to 109.4%.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
危险品化学品经营许可证（带存储）