MACROMOLECULAR RAPID COMMUNICATIONS
·
2022
·Vol. 43
·期 19
·2200124
·
IF 5.006
Kan Hu,Aoyuan Cheng,Dingcheng Zhou,Yi Luo,Guoqing Zhang
Lipid nanovesicles (LNVs) and polymer nanovesicles (PNVs), also known as liposomes and polymersomes, are becoming increasingly vital in global health. However, the two major classes of nanovesicles both exhibit their own issues that significantly limit potential applications. Here, by covalently attaching a naturally occurring phosphate “lipid head” and a synthetic polylactide “polymer tail” via facile ring-opening polymerization on a 500 g scale, a type of “chimeric” nanovesicles (CNVs) can be easily produced. Compared to LNVs, the reported CNVs exhibit reduced permeability for small and large molecules; on the other hand, the CNVs are less hydrophobic and exhibit enhanced tolerance toward proteins in buffer solutions without the need for hydrophilic polymeric corona such as poly(ethylene glycol)(PEG), in contrast to conventional PNVs. The proof-of-concept in vitro delivery experiments using hydrophilic solutions of fluorescein-PEG, rhodamine-PEG, and anti-cancer drug doxorubicin demonstrate that these CNVs, as a structurally diverse class of nano-materials, are highly promising as alternative carriers for therapeutic molecules in translational nanomedicine.
研究领域:
纳米医学
·
生物材料
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2025
·
IF 5.7
Jiuzhi Ma,Qi Feng,Zhipeng Sun,Manru Wang,Qiyuan Dai,Yue Huang,Xiaodong Cao,Qingtao Li
Bone defects have a broad impact, with over four million patients globally needing bone defect reconstruction every year due to various causes. Extracellular vesicle-functionalized scaffolds have recently emerged as a novel therapeutic approach for enhancing bone tissue regeneration. However, the clinical application of exosomes is limited by their low yield and rapid in vivo clearance. To address these challenges, we prepared nanovesicles (SCAPs-NVs) from stem cells of the apical papilla (SCAPs) using the extrusion method and loaded them into a tannic acid-Fe3+ network modified decellularized diaphragmatic tendon matrix. SCAPs can be abundantly obtained directly from extracted immature permanent teeth, are more readily available than other stem cells and also have the potential for multi-lineage differentiation. The strong interaction between tannic acid (TA) and the phospholipid bilayer of SCAPs-NVs enabled the controlled release of SCAPs-NVs. In our study, under the same conditions, the yield of SCAPs-NVs was approximately 23-fold higher than that of exosomes obtained by ultracentrifugation, highlighting the efficiency of the extrusion method. Moreover, MicroRNA sequencing of SCAPs-NVs reveals that they are enriched in angiogenic and osteogenic miRNAs. In vitro results showed that the composite scaffold loaded with SCAPs-NVs stimulated osteogenic differentiation of mesenchymal stem cells and promoted angiogenic activity of human umbilical vein endothelial cells. Micro-CT and histological evaluation confirmed the efficacy of the NVs-functionalized scaffold in promoting bone regeneration within a rat cranial defect model. This study provides novel insights into the therapeutic potential of nanovesicles, particularly SCAPs-NV, for clinical translation in bone regeneration.
研究领域:
生物材料学
·
再生医学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD ENGINEERING
·
2014
·
IF 2.576
Zhi-Wei Liu,Xin-An Zeng,Da-Wen Sun,Zhong Han
Effects of PEF on permeabilization of calcein-filled large unilamellar vesicles (LUV) with average diameter of 1705.64 ± 55.91 nm and small unilamellar vesicles (SUV) with average diameter of 213.06 ± 5.50 nm, suspended in an isosmotic sucrose solution (150 mM) were investigated. The suspensions of vesicles were exposed to PEF at various electric field strengths in the interval of 0–50 kV/cm and different treatment times ranging from 0 to 4.8 ms. The intensity of permeabilization induced by PEF was analyzed in terms of the changes of fluorescence intensity of the suspension solution and the average diameter of vesicles, due to the leakage of self-quench calcein encapsulated in vesicles. Results indicated that the permeability of LUV was increased with increasing intensity of electric field (>10 kV/cm) and treatment time, and the release percentage of calcein ( R value) was increased by 57.98% (from 0.46% to 58.44%) with the increase of the electric field strength (from 10 to 50 kV/cm) at 4.8 ms. However, the R value was only increased by 14.62% (from 43.82% to 58.44%) with the increase of the treatment time (from 1.2 to 4.8 ms) at 50 kV/cm. Average diameter of LUV was sharply decreased from 1705.64 ± 55.91 to 465.60 ± 37.12 nm with the maximum value at 50 kV/cm and 4.8 ms. Results also showed that shrinkage or rupture of LUV happened, as confirmed by the change of size distribution of LUV after treatment by PEF. However, as for SUV, the changes of the permeability of vesicles and average diameter were not observed. In addition, it was demonstrated that the magnitude of the induced transmembrane potential was proportional to the radius of the vesicle, and larger vesicles could be polarized or ruptured at relatively lower field strength than smaller vesicles.
研究领域:
生物物理学
·
食品科学与工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2017
·
IF 4.233
Huifang Xu,Na Du,Yawen Song,Shue Song,Wanguo Hou
Single-tailed short-chain alkyl keto-acids/salts, a class of fatty acid/salt derivatives, such as sodium 2-ketooctanate (KOCOONa), are a kind of weakly acid/salt type amphiphiles and plausible prebiotic molecules, and the current understanding of their aggregation behavior in aqueous solutions is still limited. Herein, the aggregation behavior of KOCOONa in aqueous solution was studied by changing its concentration ( C ), using equilibrium surface tension, conductivity, and fluorescence measurements. The aggregates formed were characterized using freeze-fracture and cryogenic transmission electron microscopy, dynamic light scattering, atomic force microscopy, and confocal laser scanning microscopy. A concentration-driven stepwise aggregation was identified in the KOCOONa solution. Vesicles can spontaneously form from the single-component aqueous solution, with a critical vesicle concentration (CVC) of ∼15 mM, which is obviously lower than that of octanoic acid/salt (120–200 mM). With increasing C , a vesicle-to-micelle transition can occur, showing a critical micelle concentration (CMC) of ∼80 mM. In addition, the membrane permeability of the KOCOONa vesicles was examined using small-size Calcein and large-size FITC-BSA as fluorescence probes, showing a size-selective permeability, similar to short-chain (C8-C11) fatty acid vesicles. For the first time, the aggregation behavior of single-tailed keto-acid salt surfactant is reported.
研究领域:
生物化学
·
分子生物学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2025
·Vol. 16
·期 1
·1-20
·
IF 15.7
Dong Huijun,Pan Zihang,Jiao Pengtao,Ye Fei,Peng Qi,Yu Yanying,Lai Xinyuan,Li Huan,Guan Zhao,Deng Juan,Shen Tao,Tan Wenjie,Shi Yi,Ding Qiang,Luo Jianyuan,Li Tong,Zhuang Hui,Xiang Kuanhui
Embryonic stem cells (ESCs) display a distinctive resistance against various viruses, irrespective of any interferon response. Nevertheless, the underlying mechanism of this resistance remains unclear. In this study, we identify vesicle-associated membrane protein 5 (VAMP5) as a potent cell-autonomous defense factor against coronaviruses, including SARS-CoV-2, with high expression levels observed in ESCs and mesoderm. VAMP5 not only exhibits functional conservation in restricting the replication of SARS-CoV-2 and its variants, as well as other highly pathogenic coronaviruses, but also shows efficacy in combating the replication of viruses from other families. Mechanistic investigations reveal that VAMP5 localizes to double membrane vesicles (DMVs) and impedes viral replication by relying on its vesicle-side C-terminal domain to interact with the viral non-structural protein 8 (NSP8), thus inhibiting the synthesis of negative-strand RNA. Our research demonstrates that VAMP5 in ESCs disrupts the protected environment of DMVs, which is essential for viral genome replication, and interacts with RNA replication complexes to defend against viral infection. This provides a novel strategy for developing broad-spectrum antiviral treatments.
研究领域:
细胞生物学
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病毒学
·
分子生物学
·
发育生物学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF SURFACTANTS AND DETERGENTS
·
2018
·Vol. 22
·期 2
·209-216
·
IF 1.454
Jun Li,Hui Li,Jie Wang,Hongshi Jiang,Chenzhong Yao,Gaofeng Wang,Huixuan Ma,Lijuan Shi
A new kind of trimeric supra-amphiphile [M-4-M-4-M][DBS] 3 that exhibits diverse self-assembly behaviours was fabricated through ion exchange between linear tricationic imidazolium chloride ([M-4-M-4-M]Cl 3 ) and sodium dodecyl benzene sulfonate (SDBS). Micelles are spontaneously transformed into unilamellar vesicles at a quite low concentration (1.5 mM), and then evolved to multilamellar vesicles and planar bilayers successively with a further increase in the concentration of [M-4-M-4-M][DBS] 3 in the absence of any additives. The presence of π-π stacking interactions has been proved to promote the lamellar arrangement of the supra-amphiphile. With increasing temperatures, [M-4-M-4-M][DBS] 3 exhibits interesting “clouding” and phase separation in the L a phase, behaving like common nonionic surfactants. Chain-like aggregates are formed in the bottom phase, which are probably induced by the hierarchical assembly of vesicles. This work provides useful insights into the role of weak interactions in the fabrication and self-assembly of oligomeric supra-amphiphiles, and paves a way to enrich the structures and functions of self-assemblies.
研究领域:
化学
·
材料科学
·
生物学
✓ 已验证
产品关联
DOI
高影响因子
Nano Today
·
2024
·
IF 13.2
Fei Sun,Shipeng Ning,Xiaoyuan Fan,Xia Wang,Ziqi Lin,Jian Zhao,Yutong Lu,Fengxiang Liu,Lili Du,Hao Zhang,Wenwen Shen,Jiaxin Lin,Zhonggui He,Kaiyuan Wang,Jin Sun
Immunotherapy for triple-negative breast cancer (TNBC) is hindered by its immunologically "cold" microenvironment, reducing treatment efficacy. Converting cold tumors into hot ones can significantly enhance the efficacy of immunotherapy. Nevertheless, the tumor extracellular matrix-associated physical barriers impede the infiltration of immunomodulating agents. Our previous research suggested that extracellular vesicles (EVs) secreted in situ by tumor cells could mediate the intercellular transport and deep infiltration of antitumor drugs. However, EVs’ application faces obstacles such as inefficient delivery, lack of specificity, low production yields, and manufacturing inconsistencies. Based on this, we explored artificial cytomembrane nanovesicles (NVs) as the biomimetics of EVs. Herein, NVs are genetically engineered with membrane fusion-promoting protein VSVG to form VSVG-NVs (V-NVs). The calcium ionophore A23187 and plasmid encoding TNF-α with Lamp2b are loaded into V-NVs (V-NVs/T+A) using electroporation technology. The fusogenic VSVG component facilitates the integration of NVs with the target cancer cells and further incorporation into secreted EVs. The A23187 enhances EVs secretion by increasing intracellular calcium level. This synergistic approach ensures efficient intracellular delivery of TNF-α through EVs, facilitating deep tumor infiltration and remodeling of the immune microenvironment. As expected, the EV-hitchhiking strategy for cascade tumor deep penetration and immune microenvironment modulation demonstrates significant potential to enhance cancer immunotherapy.
研究领域:
肿瘤学
·
免疫学
·
分子生物学
✓ 已验证
产品关联
DOI
高影响因子
Soft Matter
·
2021
·Vol. 17
·期 9
·2490-2499
·
IF 3.679
Meihua Gao,Na Du,Zhiyin Yao,Ying Li,Nan Chen,Wanguo Hou
Simple single-chain amphiphiles (SCAs) can form vesicular structures in their single-component aqueous solutions, which has attracted great attention, but the understanding of their aggregation behavior is still limited. In this work, the aggregation behavior of 4-dodecylbenzene sulfonic acid (DBSA), a typical simple SCA, in water was investigated. The structure and properties of the aggregates formed were determined. In particular, the effect of wet–dry cycles on the structures of aggregates was examined. The mechanisms of aggregate formation and structural transition were discussed. It was found that the increase of DBSA concentration can drive the occurrence of a micelle-to-vesicle transition, showing a critical micelle concentration and critical vesicle concentration of ∼0.53 and 2.14 mM, respectively. The vesicles formed coexist with micelles in solution, with a unilamellar structure and ∼80 nm size, and exhibit size-selective permeability. In addition, the vesicles show remarkable stability upon long-term storage, exposure to high temperature, and freeze–thaw cycles. The H-bonding interaction between DBSA species and the interdigitated structure of alkyl chains in bilayers play a key role in the formation and stability of DBSA vesicles. Interestingly, it was found that the wet–dry cycle can induce a micelle-to-vesicle transition and an obvious increase in the size of the original vesicles, accompanied by the formation of some multilamellar vesicles. This work provides a better understanding of the aggregation behavior of simple SCAs in their single-component aqueous solutions.
研究领域:
化学
·
材料科学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2023
·
IF 4.2
Shijie Shi,Haiqin Tang,Li Zhen,Menglin Fan,Lizhong Sun,Siying Tao,Jiaojiao Yang,Jianshu Li,Jiyao Li
Matrix vesicles (MVs), a kind of extracellular vesicles secreted by cells, play an important role in the initial stage of hard tissue mineralization. Acidic phospholipids and functional membrane proteins which are embedded into the membrane surface of MVs provide mineral nucleation sites and regulate mineral ions for facilitating biomineralization. Here, we report the MVs-mimic nanovesicles prepared from natural platelet membrane fragments. The bioinspired nanovesicles can penetrate into the deep dentinal tubule due to their stable nanostructure, and provide nucleation sites and templates for nascent mineral crystals. Meanwhile, the acidic phospholipids on the nanovesicle surface can recruit mineral ions from the environment to effectively promote biomineralization in situ. On the other hand, proteins on the surface of the nanovesicles can promote collagen cross-linking to inhibit the enzymatic hydrolysis of collagen, and further protect this mineralization template. The newly formed minerals can effectively seal the dentinal tubules, and even the sealing depth can reach 60 μm. This strategy uses “artificial MVs” derived from platelet membranes for the biomineralization of dentin, bringing new prospects for hard tissue repair in clinical practice.
研究领域:
生物材料
·
组织工程
·
牙科材料
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2017
·
IF 3.887
Xiumei Zhu,Yan Peng,Liyan Qiu
Carboplatin is one of the few platinum-based antitumor drugs used worldwide. Unfortunately, systemic carboplatin therapy is limited by its dose-related myelosuppression. In this study, a viable way to reduce the serious myelotoxicity of carboplatin while improving its therapeutic efficacy using amino-functionalized polyphosphazene vesicles is reported. First, three polyphosphazenes with different amino content were synthesized by amidating hydrophobic side groups; these polyphosphazenes could self-assemble into polymersomes with similar particle size and morphology. Based on FTIR analysis, hydrogen bonding interactions between the polymeric carriers and carboplatin were validated and shown to predominantly contribute to the significant improvement of drug loading in polymersomes. Therefore, it turned out that the amino level of polymers played a substantial role in carboplatin encapsulation. Then, PEAP-2-C polymersomes were optimized for further experimental verification to achieve depressed carboplatin-induced myelotoxicity as well as promote curative effects against CT-26 colon adenocarcinoma. These results in vitro and in vivo proved that amino-functionalized polyphosphazene vesicles have great potential for carboplatin delivery in the application of cancer therapy.
研究领域:
药学
·
纳米技术
·
癌症治疗
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 5
·1881-1890
·
IF 4.478
Wu Yike,Liu Yuanyuan,Shang Zhengjun,Liu Xin,Xu Yong,Liu Wenlan
Aberrant protein N-glycosylation is one of the hallmarks of malignancy, while its role in oral cancer (OC) progression is still poorly understood making it a perfect biomarker for early-stage cancer. In this work, we describe a MALDI-MS-based N-glycomic analysis with multivariate data analysis approach for exploration and classification of the patients with OC. Various statistical analyses were used to determine differential N-glycome profiles. There were 8 glycan structures significantly increased ( p < 0.05) in all OC-derived microvesicles (MVs). Importantly, the increases in these glycans were significantly greater in the early stage of OC than those in the advanced stage of OC. Additionally, N-glycomic profiles from the serum were also performed, demonstrating a significant change of 11 glycan structures ( p < 0.05). Our data highlighted the effectiveness approach of MALDI-MS-based MV N-glycomic profiling on OC screening, and presented strong potential for further identifying targeted glycoproteomics signatures of the cancer.
研究领域:
生物化学
·
肿瘤学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
MACROMOLECULAR RAPID COMMUNICATIONS
·
2012
·Vol. 34
·期 3
·274-279
·
IF 4.596
Jingguo Li,Hao Jiang,Wenlong Hu,Hongyan Xia,Gang Zou,Qijin Zhang
Herein, we synthesize a coumarin-substituted diacetylene monomer (CODA) and report the novel photo-controlled reversible assembly and disassembly behavior of the polymerized CODA (PCODA) vesicles. The photo-triggered dimerization and cleavage reactions of the coumarin groups within the surface of the adjacent PCODA vesicles can be utilized as the driving force to induce assembly and disassembly of PCODA vesicles. Moreover, the boundary of PCODA vesicles in the aggregates becomes more obscure when the irradiation time exceeds 30 min. Fusion occurs upon close docking of target membranes, driven by sufficient dimerization of the coumarin groups within the surface of PCODA vesicles.
研究领域:
材料科学
·
生物化学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）