NEW JOURNAL OF CHEMISTRY
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2021
·Vol. 45
·期 15
·6780-6786
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IF 3.591
Ling Liang,Zhenghong Zhao,Fanggui Ye,Shulin Zhao
Dopamine (DA) exists as a meaningful neurotransmitter which is extremely useful for the diagnosis of neurological disorders. For this reason, monitoring the levels of DA is greatly important. A facile colorimetric method is described here for measuring DA using tetravalent cerium ions (Ce4+). Inspired by the intrinsic oxidase-like activity of Ce4+, it can directly oxidize the substrate 3,3,5,5-tetramethylbenzidine (TMB) in the presence of oxygen (O2) without requiring hydrogen peroxide (H2O2). Interestingly, when DA is introduced, the catalytic oxidation ability of Ce4+ is significantly enhanced, and a dark blue color of oxidized TMB (oxTMB) can be observed instantly. This boost is benefited from the fact that more superoxide radicals (˙O2−) are generated with the addition of DA. In view of this, a facile approach for colorimetric determination of DA has been realized. Within the range of 6.5–520 nM, the absorption intensity of oxTMB is proportional to the concentration of DA and the detection limit is 2.88 nM. What is more, the detection can be accomplished in 3 min at room temperature, which is extremely important for real-time and rapid bioanalysis. This sensor has been successfully applied to measure DA in biological samples. Such an economical, simple, rapid, and sensitive method shows great potential application prospects in clinical early diagnosis.
研究领域:
分析化学
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神经生物学
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临床诊断
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产品关联
DOI
高影响因子
ACS Omega
·
2018
·
IF 0
Shengli Wang,Yanping Jia,Lianfa Song,Haifeng Zhang
Hydroxylamine (HA) can react with hydrogen peroxide (H2O2) to generate hydroxyl radical (HO•), but the reaction rate between them is very slow (2.2 × 10–4 M–1 s–1). We propose a new system to accelerate the formation of aminoxyl radical (NH2O•) by the addition of cerium [Ce(IV)] to induce the continuous production of HO• through reaction with H2O2. We also investigate the decolorization and mineralization of rhodamine B (RhB) and mechanism in the Ce(IV)/H2O2/HA system. The initial pH plays a significant role in decolorization of RhB. In this work, observation of the rapid decolorization process after 60 min revealed that approximately 80% of RhB was degraded at the initial pH of 4.0. The HO• radicals were considered as the primary reactive oxidant in the system, during its investigation through coumarin capturing, benzoic acid capturing, and radical quenching experiments. The results of the present study suggest that the addition of Ce(IV) can greatly enhance the production of HO•, and the rapid decolorization and mineralization of RhB can occur through the Ce(IV)/H2O2/HA system at acidic pH conditions.
研究领域:
环境化学
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水处理
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高级氧化过程
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产品关联
DOI
PubMed
CHEMISTRY & BIODIVERSITY
·
2023
·Vol. 20
·期 3
·e202200813
·
IF 2.9
Kaiwen Li,Jie Wang,Zizhen Huang,Chunxia Tan
Angelica Sinensis polysaccharide cerium (ASP−Ce) was prepared by Angelica Sinensis polysaccharide (ASP) and cerium ammonium nitrate (NH 4 ) 2 Ce(NO 3 ) 6 . and its morphology, and solid structure was investigated. The antioxidant activity of the ASP−Ce complex in vitro was evaluated. The antioxidant activity of ASP−Ce complex in vitro was evaluated by the scavenging activity of 2,2-diphenyl-1-picrylhyrazyl radical (DPPH), hydroxyl radical (⋅OH) and superoxide anion radical ( ⋅). The results showed that the ASP−Ce had the more ordered structure for inserting the Ce 4+ into the polymer chain of ASP and there was little change in the conformation of the polysaccharide from Ce 4+ . Three free radical scavenging experiments proved that ASP−Ce had better antioxidant capacity than of ASP, especially on DPPH, and then on ⋅. The scavenging rate of ASP−Ce at 1.0 mg/mL on DPPH reached 71.6 %. Therefore, these results provide references for the further development and utilization of rare earth-polysaccharide.
研究领域:
药理学
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材料科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2021
·
IF 6.057
Tingting Pan,Ting Zhou,Yifeng Tu,Jilin Yan
A fluorescence method was developed for a turn-on measurement of acid phosphatase (ACP) activity. It was found that cerous ion (Ce 3+ ) could lead to an enhancement of glutathione protected gold nanocluster fluorescence through an aggregation-induced-emission (AIE) process, while its higher valent counterpart ceric ion (Ce 4+ ) could not. In a weakly acidic environment, ACP catalyzed the dephosphorylation of a phosphate ester of ascorbic acid , with a generation of ascorbic acid (AA). AA reduced Ce 4+ into Ce 3+ , which subsequently enhanced the nanocluster fluorescence. This kind of turn-on fluorescence linearly related to the ACP activity in the range of 0.005–2.4 U/L, with a limit of detection as 0.001 U/L. Human serum samples were measured after a trichloroacetic acid treatment and a simple dilution. The whole analyses were accomplished in 1.5 h with results in good accordance with a reference method.
研究领域:
分析化学
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生物化学
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纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2025
·
IF 4.5
Qian-ni Wu,Zi-ren Feng,Qin Tang,Jiu-ping Jin,Xue-hui Liu,Zhi-bin Mai,Sheng-nan Zhao,Yong-qi Lan,Kai-xin Chen,Jin-duan Lin,Peng-cheng Xu,Ji-jun Fu
Acute liver injury (ALI), often triggered by an acetaminophen (APAP) overdose, is characterized by severe oxidative stress, inflammation, and hepatocyte apoptosis. Current therapies, such as N-acetylcysteine (NAC), are limited by narrow treatment windows, highlighting the need for more effective antioxidant strategies. In this study, cerium oxide nanoparticles (CeO2 NPs, nanoceria) were synthesized and comprehensively characterized using the transmission electron microscopy (TEM), dynamic light scattering method (DLS), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) to confirm their branched morphology, high crystallinity, and mixed Ce3+/Ce4+ valence states. Their enzyme-mimetic antioxidant activities were evaluated through superoxide, hydrogen peroxide, and hydroxyl radical scavenging assays. Nanoceria exhibited excellent cytocompatibility and effectively suppressed the generation of lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) and lipid peroxidation and caspase-3-mediated apoptosis in macrophages. They also downregulated pro-inflammatory mediators (nitric oxide synthase (iNOS), TNF-α, IL-1β, and NLRP3) while enhancing anti-inflammatory markers (Arg1 and IL-10). In an APAP-induced ALI mouse model, nanoceria preferentially accumulated in the liver, alleviated oxidative stress and inflammation, and significantly reduced aspartate aminotransferase (AST) levels, showing hepatoprotective efficacy comparable to NAC. Nanoceria protect against APAP-induced ALI via synergistic antioxidative and anti-inflammatory mechanisms based on reversible Ce3+/Ce4+ redox cycling. These findings underscore nanoceria’s potential as a next-generation nanotherapeutic for oxidative stress-related liver diseases.
研究领域:
纳米医学
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毒理学
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肝病学
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氧化应激
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炎症
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Advanced Science
·
2024
·
IF 14.3
Jieran Yao,Zhenhong Luo,Jiaying Lin,Na Meng,Jiangna Guo,Hui Xu,Rongwei Shi,Linhui Zhao,Jiateng Zhou,Feng Yan,Bin Wang,Hailei Mao
The escalating spread of drug-resistant bacteria and viruses is a grave concern for global health. Nucleic acids dominate the drug-resistance and transmission of pathogenic microbes. Here, imidazolium-type poly(ionic liquid)/porphyrin (PIL-P) based electrospun nanofibrous membrane and its cerium (IV) ion complex (PIL-P-Ce) are developed. The obtained PIL-P-Ce membrane exhibits high and stable efficiency in eradicating various microorganisms (bacteria, fungi, and viruses) and decomposing microbial antibiotic resistance genes and viral nucleic acids under light. The nuclease-mimetic and photocatalytic mechanisms of the PIL-P-Ce are elucidated. Co-infection wound models in mice with methicillin-resistant S. aureus and hepatitis B virus demonstrate that PIL-P-Ce integrate the triple effects of cationic polymer, photocatalysis, and nuclease-mimetic activities. As revealed by proteomic analysis, PIL-P-Ce shows minimal phototoxicity to normal tissues. Hence, PIL-P-Ce has potential as a “green” wound dressing to curb the spread of drug-resistant bacteria and viruses in clinical settings.
研究领域:
生物技术
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纳米技术
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微生物学
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药学
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材料科学
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医学研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）