ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Fan Xinyu,Zhou Lin,Xing Yanchao,Wang Liming,Choi Shin Sik,Zhang Zixin,Zhang Xu,Liu Caixiang,Zhu Yu,Fu Zhifei,Han Lifeng
The processing of traditional Chinese medicine (TCM) plays an important role in the clinical application, which usually has the function of "increasing efficiency and reducing toxicity". Polygonum multiflorum (PM) has been reported to induce hepatotoxicity, while it is believed that the toxicity is reduced after processing. Studies have shown that the hepatotoxicity of PM is closely related to the changes in chemical components before and after processing. However, there is no comprehensive investigation on the chemical changes of PM during the processing progress. In this research, we established a comprehensive method to profile both small molecule compounds and polysaccharides from raw and different processed PM samples. In detail, an online two-dimensional liquid chromatography coupled with quadrupole-orbitrap mass spectrometry (2D-LC/Q-Orbitrap MS) was utilized to investigate the small molecules, and a total of 150 compounds were characterized successfully. After multivariate statistical analysis, 49 differential compounds between raw and processed products were screened out. Furthermore, an accurate and comprehensive method for quantification of differential compounds in PM samples was established based on ultra-high performance liquid chromatography/Q-Orbitrap-MS (UHPLC/Q-Orbitrap-MS) within 16 min. In addition, the changes of polysaccharides in different PM samples were analyzed, and it was found that the addition of black beans and steaming times would affect the content and composition of polysaccharides in PM significantly. Our work provided a reference basis for revealing the scientific connotation of the processing technology and increasing the quality control and safety of PM. Graphical Abstract
研究领域:
药理学
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分析化学
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中医药
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毒理学
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产品关联
DOI
PubMed
高影响因子
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT
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2012
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IF 1.207
Changjuan Guo,Zhongyao Jiang,Chunguang Xie,Hui Zhu,Wei Gao,Zhengxu Huang,Ping Cheng,Zhong Fu,Zhen Zhou
According to the demand of home-made spectrometer, a radio frequency (RF) signal driver was developed. This RF signal driver is composed of signal generating circuit, signal amplification circuit and power output circuit, to drive the radio frequency quadrupole (RFQ). The designed RFQ is used to transfer ions generated in atmospheric pressure ion source to a home-made desktop orthogonal-injection time-of-flight mass analyzer. This signal driver is divided into low-frequency part and high-frequency part to support RFQ transferring ions of larger values (i.e., m / z =100–600) and smaller values (i.e., m / z =20–100) respectively. The low-frequency part of the RF signal driver can provide RF signals with resonance frequency of 1.43 MHz, peak to peak voltage V p–p of 0–1080 V, and the high-frequency part can provide RF signals with resonance frequency of 2.05 MHz, peak to peak voltage V p–p of 0–520 V. With the radio frequency signal driver described in this paper, ions in the range m/z 20–600 can be transmitted efficiently by RFQ.
研究领域:
分析化学
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质谱技术
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2014
·Vol. 407
·期 6
·1595-1605
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IF 3.578
Du Yan,Wu Dapeng,Wu Qian,Guan Yafeng
In this study, the efficiency of five peptide-extraction methods—acetonitrile (ACN) precipitation, ultrafiltration, C18 solid-phase extraction (SPE), dispersed SPE with mesoporous carbon CMK-3, and mesoporous silica MCM-41—was quantitatively investigated. With 28 tryptic peptides as target analytes, these methods were evaluated on the basis of recovery and reproducibility by using high-performance liquid chromatography–triple-quad tandem mass spectrometry in selected-reaction-monitoring mode. Because of the distinct extraction mechanisms of the methods, their preferences for extracting peptides of different properties were revealed to be quite different, usually depending on the pI values or hydrophobicity of peptides. When target peptides were spiked in bovine serum albumin (BSA) solution, the extraction efficiency of all the methods except ACN precipitation changed significantly. The binding of BSA with target peptides and nonspecific adsorption on adsorbents were believed to be the ways through which BSA affected the extraction behavior. When spiked in plasma, the performance of all five methods deteriorated substantially, with the number of peptides having recoveries exceeding 70 % being 15 for ACN precipitation, and none for the other methods. Finally, the methods were evaluated in terms of the number of identified peptides for extraction of endogenous plasma peptides. Only ultrafiltration and CMK-3 dispersed SPE performed differently from the quantitative results with target peptides, and the wider distribution of the properties of endogenous peptides was believed to be the main reason.
Distribution of the recoveries of target peptides after treatment by the five peptide-extraction methods
研究领域:
生物化学
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分析化学
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蛋白质组学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2017
·Vol. 98
·期 4
·1574-1583
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IF 2.463
Shanshan Yu,Yufan Zhang,Yun Ran,Wenyi Lai,Zhaoshou Ran,Jilin Xu,Chengxu Zhou,Xiaojun Yan
BACKGROUND Steryl glycosides (SGs) are sterol conjugates found in various plants, especially in those making up human diets. It has been demonstrated that SGs have potential health benefits, and they could be used as food supplements in a variety of food matrixes. Marine microalgae are a potential resource for human food and ingredients. In this study, gas chromatography–triple quadrupole mass spectrometry (GC–QQQ-MS) was used to characterize unknown SGs in eight microalgae belonging to different classes ( Isochrysis galbana 3011, Pavlova viridis , Platymonas helgolandica , Conticribra weissflogii , Thalassiosira pseudonana , Nitzschia closterium , Gymnodinium sp., and Karlodinum veneficum ). RESULTS The SGs were first extracted from lyophilized algae with chloroform–methanol, purified by solid-phase extraction and analyzed as trimethylsilyl derivatives. Nine SGs have been identified. In particular, new SGs like occelasteryl glycoside and stellasteryl glycoside were found in Gymnodinium sp., 24-methylene cholesteryl glycoside was detected in P. helgolandica , and 4,24-dimethylcholestan-3-yl glycoside was identified as the main constituent of microalga K. veneficum . The results also showed that the compositions of SGs in different microalgae varied, with a range of 5.234 to 0.036 g kg −1 , and microalga P. viridis contained the most abundant SGs. CONCLUSION GC–QQQ-MS is a powerful tool to detect SGs with different structures from a variety of microalgae. The compositions of SGs in different microalgae varied greatly. Microalgae are a good source of highly valued SGs. © 2017 Society of Chemical Industry
研究领域:
海洋生物学
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分析化学
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食品科学
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产品关联
DOI
PubMed
高影响因子
Journal of Analytical Methods in Chemistry
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2024
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IF 2.3
Mingwen Zhang,Yunqiang Zhao,Zongwen Wang,Jintian Cheng
A fast, simple, and sensitive method for the simultaneous determination of seven polyamines in Nephotettix cincticeps was developed based on ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-3Q-MS) together with liquid phase extraction. Polyamines in insect samples were extracted with HClO4 solution and then were separated and detected by UPLC-3Q-MS, which was equipped with a hydrophilic interaction liquid chromatography column, within 5 min without any derivatization procedure. The method has been successfully used to detect 7 polyamines in healthy and difluormethylornithine-treated adults of Nephotettix cincticeps with a method limit of detection and the method limit of quantitation of 24–139 pg/mg and 82–464 pg/mg, respectively, an intraday and interday relative standard deviation (RSD, n = 5) of 1.97–6.00% and 2.08–5.92% respectively, and a recovery of 86–115%. The success of this study provided a reliable method for the rapid and high-throughput detection of polyamines in the insect sample.
研究领域:
分析化学
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昆虫学
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生物化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Dejin Wang,Xingqiang Wu,Jingchun Mao,Zongyi Wang,Yujie Xie,Xudong Wu,Kaixuan Tong,Hongping Man,Chunlin Fan,Hui Chen
In this work, a simple, high-throughput, and sensitive analytical method for simultaneously determining 270 pesticides and 13 mycotoxins from different chemical classes in raw coffee beans was developed. The modified quick, easy, cheap, effective, rugged, and safe (QuEChERS) process mainly involved the effects of hydration volume, extraction solvent, extraction salt, and the type and amount of adsorbent used for sample extraction and purification. The samples were detected by liquid chromatography-quadrupole-time-of-flight mass spectrometry (LC-Q-TOF/MS) with a self-built database. The method effectively reduced matrix effects and the 283 compounds showed good linearity in their respective linear ranges ( R 2 > 0.99). The screening detection limits (SDL) of the method were in the range of 0.1–20 μg/kg, and the limits of quantification (LOQ) were in the range of 0.2–20 μg/kg. The average recoveries of the 283 compounds at three spiked levels ranged from 72.1 % to 114.9 %, with intra-day and inter-day relative standard deviations less than 9.8 % and 17.1 %, respectively. The recoveries of all analytes ranged from 70.0 % to 120.0 % at three spiked levels (1×LOQ, 2×LOQ, and 10×LOQ), with relative standard deviations less than 20.0 %. The results were satisfactory according to the SANTE/11312/2021 guidelines. The method successfully analyzed 34 batches of actual samples, with concentrations ranging from 0.1 to 61.9 μg/kg for pesticides and 2.3–43.0 μg/kg for mycotoxins. This method provides a powerful tool for monitoring organic contaminants in raw coffee beans.
研究领域:
食品科学
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分析化学
✓ 已验证
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2024
·Vol. 47
·期 21
·e70019
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IF 2.8
Gang Chen,Nan Ge,Le Yang,Hui Sun,Ye Sun,Guangli Yan,Ying Han,Xiang Li,Xijun Wang
Guyinjian (GYJ) is a classic traditional Chinese medicine formula for the treatment of polycystic ovary syndrome, ovulation disorders, immuno-sterility, oligomenorrhea with kidney deficiency, and so forth. Due to the unclear chemical components of GYJ, the elucidation of the pharmacological mechanism, and the comprehensive development and utilization of GYJ in clinical is being restricted. In this study, ultra-high-performance liquid chromatography with quadrupole time-of-flight-tandem mass spectrometry combined with the Progenesis QI platform and using reference standards, online and self-built databases matching, fragmentation rules analysis of mass spectrometry peaks, a total of 235 compounds were preliminarily characterized, including 69 flavonoids, 65 terpenoids, 34 phenylpropanoids, 22 saccharides, sugar esters, and glycosides, 15 organic acids, and 30 others. These compounds may reflect the chemical properties of GYJ. The method established in this study can systematically, rapidly, and accurately resolve the chemical components in GYJ, which lays the foundation for further establishment of the pharmacodynamic substance basis and quality control.
研究领域:
中医药学
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分析化学
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药理学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2023
·Vol. 15
·期 26
·3206-3224
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IF 3.1
Yunxi Liu,Tianyu Dong,Kuan Yan,Zhanliang Wang,Genye He,Yufeng Zhang,Congcong Ma,Lu Liu,Wei Chang,Lisi Zhang
Since the World Anti-Doping Agency's (WADA) Prohibited List is updated on an annual basis, screening methods must be continually adapted to align with these changes. In accordance with Technical Document-MRPL 2022, a newly combined, comprehensive, rapid and high-throughput doping control screening method has been developed for the analysis of 350 substances with different polarities in human urine using ultra-high performance liquid chromatography coupled with Q Exactive Plus Hybrid Quadrupole-Orbitrap mass spectrometer (UPLC-QE Plus-HRMS) and ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometer (UPLC-QQQ-MS). The limits of detection were in the range of 0.12–50 ng mL−1 for beta-2 agonists, hormone and metabolic modulators, narcotics, cannabinoids and glucocorticoids, 0.1–14 ng mL−1 for the manipulation of blood and blood components, beta blockers, anabolic agents and hypoxia-inducible factor (HIF) activating agents, and 2.5–100 000 ng mL−1 for substances of Appendix A, diuretics & masking agents and stimulants. The sample preparation consisted of two parts: one is the dilute & shoot part analyzed in UPLC-QQQ-MS, another is a mixture of the dilute & shoot part and a liquid–liquid extraction part of hydrolyzed human urine analyzed in UPLC-QE Plus-HRMS in full scan mode with polarity switching and parallel reaction monitoring (PRM) mode. The method has been fully validated for doping control purposes. All the substances were compliant with WADA's required 1/2 minimum requirement performance level (MRPL) or minimum reporting level (MRL), and this method was successfully used in the 2022 Beijing Winter Olympic Games and Winter Paralympic Games for anti-doping purpose.
研究领域:
分析化学
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运动科学
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药理学
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法医学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Li Wang,Yating Sun,Linjing Dong,Yuanyuan Li,Qin Zhang,Ye Ding,Jieshu Wu,Zhixu Wang
Polyamines in human milk are essential for the rapid growth and development of neonates and infants. However, the concentrations of polyamines in human milk have not been well characterized and the special methods for detecting polyamines in human milk, formula milk, or other dairy products remain largely unavailable to date. In this study, an accurate and sensitive method based on trifluoroacetic acid anhydride (TFAA) derivatization, double solid-phase extraction (SPE) purification, and gas chromatography–triple quadrupole mass spectrometry (GC-QqQ MS) was established for the evaluation of polyamines in human milk, formula milk, and other dairy products. High coefficients of determination for internal calibration curves were obtained (R2 > 0.99). Low limits of detection (5.0, 1.0, and 0.5 nmol/dL) and limits of quantification (10.0, 3.0, and 1.0 nmol/dL) for putrescine, spermidine, and spermine, respectively, were also achieved. The precision (expressed as relative standard deviation, RSD < 9.0%) and recovery rates (78.3–108.4%) were satisfactory. Spermidine and spermine in human milk were found significantly higher than those in formula milk and commercial cow’s milk. The difference of polyamine content in human milk and infant formula should be paid attention to, particularly when infant formula is used as a complete replacement for human milk.
研究领域:
营养学
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分析化学
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儿科医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2022
·
IF 3.571
Jinxin Zhou,Sheng Yu,Bingqian Wang,Xing Wei,Li Zhang,Mingqiu Shan
Yihuang decoction (YHD) is one of the most famous formulas in tradition Chinese medicine (TCM) and has been clinically used for treatment of vaginitis , pelvic inflammation and other gynecological diseases for hundreds of years. However, its chemical composition remains unclear. In this study, high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF-MS) was employed for its chemical profiling investigation. As a result, 90 components were chemically defined, including 23 alkaloids, 14 organic acids , 3 phenylethanoid glycosides , 4 iridoid glycosides, 5 terpenoid lactones , 10 flavonoids , 8 nucleobases and nucleosides , 12 amino acids , and 11 other compounds. In addition, 8 representative compounds (acteoside, allantoin , berberine , 4- O -feruloylquinic acid, 5- O -feruloylquinic acid, gallic acid , geniposidic acid , and phellodendrine) were simultaneously determined in 10 batches of YHD samples by HPLC with a diode array detector (HPLC-DAD). For all the analytes, their calibration curves showed good linearity (R 2 >0.9990) within the test ranges. RSDs of precision, repeatability and stability test were all below 3.50%. The overall recoveries ranged from 93.63% to 105.02%, with RSDs less than 3.50%. This study is supposed to exhibit a comprehensive chemical profiling of YHD and to provide some strong basis for quality control and even for action mechanism of this ancient classical prescription.
研究领域:
中药化学
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分析化学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2016
·Vol. 39
·期 10
·1853-1862
·
IF 2.741
Xianrui Liang,Qiao Xu
A validated method based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was established to separate and identify phenolic compounds in Bidens pilosa L. Mass spectrometry experiments were performed both in positive and negative ion modes. A total of 35 compounds were detected, and 26 phenolic compounds were unequivocally identified or tentatively assigned based on retention time, maximum UV absorption, molecular formula, and fragments. The ultra high performance liquid chromatography method was validated and showed good linearity ( R 2 ≧ 0.9996) over the test range. The limits of detection and quantification were above 0.072 and 0.162 μg/mL, respectively. The relative standard deviations of intraday and interday precision were below 0.3 and 1.6%, respectively.
研究领域:
植物化学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Zhihong Shi,Jing Jiang,Weiyue Pang,Hongyu Ma,Xiaoxue Chu,Can Zhou,Hongyi Zhang
In this study, carbon fibers (CFs) were used as sorbent material for the dispersive micro-solid phase extraction (d-μSPE) of anthracene , acenaphthene and phenanthrene from tea infusion samples prior to gas chromatography-quadrupole mass spectrometric detection. CFs were obtained by carbonizing cotton at 800 °C under the protection of nitrogen for 4 h. After characterization by the scanning electron microscopy-energy dispersive spectrometry, X-ray diffraction and N 2 adsorption-desorption, 20 mg of CFs were used to effectively adsorb PAHs in 5 min from 10 mL of tea infusion samples, 2 mL of toluene was used as desorbent . Under the optimum conditions, good linearity was obtained with R 2 between 0.9996 and 0.9998. The limits of detection were in the range of 0.012–0.014 μg/L. RSD values for intra- and inter-day precision were ≤6.7% and ≤7.8%, respectively. In this work, cotton based CFs were prepared in a solventless and green way and the CFs based d-μSPE procedure was simple, rapid and cost-effective, with no need of column-packing. The proposed method has been successfully applied to the analysis of PAHs in three kinds of tea infusion samples.
研究领域:
分析化学
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环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）