APPLIED CATALYSIS B-ENVIRONMENTAL
·
2022
·
IF 24.319
Shuisen He,Tingting Huang,Yu Fan
Novel alumina-supported Ni-Mo hydrodesulfurization (HDS) catalysts were prepared via assembly of Mo and Al precursors induced by tetradecylamine (TDA). Compared with conventional impregnation, this assembly method improves Mo dispersion, weakens metal-support interactions, enhances Ni modification at MoS 2 edge sites, and produces more NiMoS phases on Al 2 O 3 . The optimal NiMoS microstructure was obtained by modulating the TDA/Mo ratio in the assembly. A microstructure-activity correlation indicated that the S vacancies at the NiMoS corner sites favored σ-adsorption and direct desulfurization of 4,6-dimethylbenzothiophene (4,6-DMDBT), and those at the NiMoS edge sites promoted C-S breaking in hydrogenated derivatives of 4,6-DMDBT. Among these catalysts, NiTMo-2.0/Al 2 O 3 , which had the most corner and edge S vacancies, exhibited the highest reaction rate constant of 5.89 × 10 −7 mol·g −1 ·s −1 and the highest desulfurization ratio of 99.5% in HDS of 4,6-DMDBT.
研究领域:
催化化学
·
能源化工
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DOI
高影响因子
ENVIRONMENTAL TOXICOLOGY
·
2025
·
IF 3.2
Yang Li,Keyuan Zhong,Sijie Zhang,Shiyi Duan,Kaijie Huang,Xiaofang Che,Xinchun Guo,Huiqiang Lu
D-tetramethrin is a common insecticide that is important for the control of mosquito-transmitted diseases such as malaria and dengue and, due to its widespread use, it is frequently detected in environmental systems. However, there is currently very little information on the influence of D-tetramethrin on non-target organisms. In this study, zebrafish embryos were exposed to various concentrations of D-tetramethrin (0, 3, 6, and 9 mg/L) from 6 to 72 h past fertilization (hpf) to ascertain the influence of D-tetramethrin on the zebrafish immune system and development. We found that D-tetramethrin exposure led to a significant decrease in heart rate, an increase in the yolk area and tail flick frequency, and a shortening of body length in zebrafish larvae, compared with the control group. The number of macrophages and neutrophils in the experimental group of zebrafish larvae decreased significantly and the oxidative stress levels increased compared with the control group. The malondialdehyde (MDA) content and reactive oxygen species (ROS) content increased significantly, while catalase (CAT) activity increased and superoxide dismutase (SOD) activity decreased in the experimental group. At the same time, apoptosis increased in the zebrafish embryo cells, and the expression of apoptosis-related genes such as p53 , Bax , and Bcl-2 was abnormal. Moreover, the pro-inflammatory genes IL-8 , IFN-γ , IL-6 , and TNF-α were up-regulated, while the anti-inflammatory gene, IL-10 , was down-regulated. Therefore, D-tetramethrin significantly affects the immune system and oxidative stress levels in zebrafish, inducing cell apoptosis and negatively affecting embryo development. These results provide novel data for the toxicity of D-tetramethrin and the potential adverse effects of environment residues.
研究领域:
环境毒理学
·
发育生物学
·
免疫学
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DOI
PubMed
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-7
·
IF 5.228
Guo Longhua,Xu Shaohua,Ma Xiaoming,Qiu Bin,Lin Zhenyu,Chen Guonan
Colorimetric enzyme-linked immunosorbent assay utilizing 3′-3-5′-5-tetramethylbenzidine(TMB) as the chromogenic substrate has been widely used in the hospital for the detection of all kinds of disease biomarkers. Herein, we demonstrate a strategy to change this single-color display into dual-color responses to improve the accuracy of visual inspection. Our investigation firstly reveals that oxidation state of 3′-3-5′-5-tetramethylbenzidine (TMB 2+ ) can quantitatively etch gold nanoparticles. Therefore, the incorporation of gold nanoparticles into a commercial TMB-based ELISA kit could generate dual-color responses: the solution color varied gradually from wine red (absorption peak located at ~530 nm) to colorless, and then from colorless to yellow (absorption peak located at ~450 nm) with the increase amount of targets. These dual-color responses effectively improved the sensitivity as well as the accuracy of visual inspection. For example, the proposed dual-color plasmonic ELISA is demonstrated for the detection of prostate-specific antigen (PSA) in human serum with a visual limit of detection (LOD) as low as 0.0093 ng/mL.
研究领域:
生物医学工程
·
诊断学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Yu Liu,Fangfang Wang,Yawen Liu,Lu Cao,Haiming Hu,Xiaowei Yao,Junping Zheng,Hongtao Liu
Label-free gold nanoparticles (AuNPs) plasmonic sensors are limited in the biological assay for weak expansibility. Here, a novel H 2 O 2 -sensing AuNPs plasmonic sensor was proposed using 3, 3′, 5, 5′-tetramethylbenzidine (TMB) redox catalysis of H 2 O 2 -indcued cysteine oxidation for AuNPs coloration (TreHOAuC), and the application of TreHOAuC was examined by combining H 2 O 2 -generating oxidase. In the TreHOAuC platform, TMB is transformed into oxidized TMB (oxTMB) by horseradish peroxidase in the presence of H 2 O 2 , and oxTMB is restored into TMB after oxidizing cysteine into cystine. The redox cycling of TMB boosts H 2 O 2 -induced cysteine oxidation, and the cysteine oxidation is visualized by AuNPs. Critical parameters of the TreHOAuC platform were optimized such as concentration, time, pH, and buffer. Using the TreHOAuC system, H 2 O 2 can be specifically sensed by naked eyes within 2 min, and the detection limit was less than 0.5 μM. As a proof-in-conception, a cholesterol oxidase-paired TreHOAuC system was used to detect cholesterol in serum samples, indicating a linear range of 2–40 μM and great preciseness. In summary, our proposed TreHOAuC method provided a novel and more comprehensive application for biological sensing.
研究领域:
纳米技术
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分析化学
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生物医学工程
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产品关联
DOI
高影响因子
Greenhouse Gases-Science and Technology
·
2022
·Vol. 12
·期 2
·305-320
·
IF 2.518
Zichen Liu,Shuman Xu,Jing Hao,Lina Song,Mingben Chong,Dang-Guo Cheng,Fengqiu Chen
The conversion of CO 2 into high-value chemicals has drawn a great deal of consideration, of which the most ideal product is lower olefins. However, the modified Fischer–Tropsch (F-T) synthesis with CO as an intermediate shows poor selectivity for lower olefins. Here, a list of bifunctional catalysts consisting of SAPO-34 zeolites with adjustable silicon contents and In 2 O 3 /ZrO 2 were synthesized. In the reaction process, CO 2 is first hydrogenated to methanol over an In 2 O 3 /ZrO 2 catalyst, and then the generated methanol rapidly diffuses to the adjacent zeolite catalyst to react to lower olefins. The selectivity of lower olefins (88.3%) and conversion rate of CO 2 (23.3%) was achieved over In 2 O 3 /ZrO 2 and SAPO-34-0.05 at 633 K, a gas hourly space velocity (GHSV) of 4500 ml·gcat –1 ·h –1 , and H 2 /CO 2 /Ar = 3:1:1. The catalytic performance remained stable after 50 h of reaction. Characterization analysis showed that Si content affected the content of Brønsted acid sites (BAS) and morphology of SAPO-34, resulting in different distribution of reaction intermediates. Tetramethyl-benzenes, as important intermediate species, had a slower conversion rate on the SAPO-34 with low Si content and nanosheet morphology, which led to high lower olefins selectivity. © 2022 Society of Chemical Industry and John Wiley & Sons, Ltd.
研究领域:
催化
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化学工程
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材料科学
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环境化学
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DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2019
·Vol. 411
·期 18
·4063-4071
·
IF 3.286
Lin Shan,Zheng Danmin,Li Ailing,Chi Yuwu
It is well known that 3,3′,5,5′-tetramethylbenzidine (TMB) can be oxidized into blue or yellow oxidzed TMB (oxTMB) with the catalysis of peroxidase or mimetic enzyme of platinum nanoparticles (Pt NPs). In this work, we found that TMB could be oxidized into very stable black oxTMB with the catalysis of Pt NPs under certain chromogenic reaction conditions. For the first time, the black oxTMB was revealed to consist of nanowires (oxTMB NWs) with lengths of more than 100 μm and diameters of around 100 nm. The black oxTMB NWs showed very strong light absorption ability, thus could be used to greatly amplify the signal of Pt NP-based immunochromatography test strips (ICTSs). The Pt NP-based ICTSs with black oxTMB NW signal amplification have shown much better assay ability (linear response range and limit of detection) than those of gold nanoparticle (Au NP)-based ICTS, Pt NP-based ICTS, and Pt NP-based ICTS with blue or yellow oxTMB signal amplifications. The developed Pt NP-oxTMB NW-based ICTS has been demonstrated to be a new, accurate, sensitive, selective, and rapid immunosensor for quantitative detection of antigens such as human chorionic gonadotropin (HCG).
研究领域:
分析化学
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纳米技术
·
生物传感器
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 2
·1-7
·
IF 6.408
Zhang Bing,Wang Xue,Cheng Yan
A photochromic immunoassay was built for tumor marker detection based on ZnO/AgI nanophotocatalyst. Frist, ZnO/AgI nanoparticles were synthesized and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray powder diffraction (XRD), and Fourier transform infrared spectrometry (FTIR). The color development is caused by tetramethyl benzidine (TMB) solution oxidated by ZnO/AgI nanomaterials. The electron transitions in ZnO/AgI nanomaterials are driven by visible light irradiation, generating photogenerated hole and oxidizing TMB to blue solution. Appropriate band width between ZnO and AgI promotes separation of photogenerated electrons and holes and enhances oxidation efficiency. A sandwich-type immunoassay was constructed based on ZnO/AgI nanomaterial as labels. The absorbance at 650 nm of reaction solution is positively correlated with antigen concentration. The developed immunoassay showed good performance for carcinoma embryonic antigen (CEA) detection in the range 0.1–7.0 ng/mL with a detection limit of 65 pg/mL. The photochromic immunoassay also exhibited preferable selectivity, repeatability, and stability. Graphical abstract A novel colorimetric immunoassay was constructed based on ZnO/AgI photocatalyst. ZnO/AgI nanomaterials occur electron transitions under visible light irradiation and generate photogenerated hole, which can oxidize TMB to blue solution. Carcinoembryonic antigen in sample was detected sensitively due to the high catalytic efficiency of ZnO/AgI nanomaterials.
研究领域:
生物化学
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纳米技术
·
分析化学
·
癌症研究
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Yingxue Li,Tingrui Zhang,Gang Bai,Mengchun Chen,Xiaodong Lei,Li Ye,Hua Yu,Zhichao Fan,Tao Yu
MicroRNAs (miRNAs) play an important role in the process of heart failure (HF) and are emerging biomarkers that can be used for the auxiliary diagnosis of HF. However, it is very challenging to accurately analyze the expression levels of trace miRNAs in complex clinical samples. Here, we developed an enzyme-free colorimetric sensor for the ultrasensitive detection of miRNA-423-5p (HF-associated miRNA) based on three-dimensional DNA walkers constructed from functional nucleic acids and gold nanoparticles (AuNPs). DNAzyme with cleavage activity was specifically activated by miRNA-423-5p to sustainably cleave the substrate, thereby releasing the trigger sequence to initiate the subsequent mismatched catalytic hairpin assembly (MCHA) cycle. Then, as the MCHA cycle proceeded to continuously expose the G-quadruplex (GQ) sequence, the sequence bound with hemin to form a large amount of GQ/hemin DNAzyme on the surface of the AuNPs, which rapidly catalyzed the chromogenic oxidation of 3,3′,5,5′-tetramethylbenzidine to yield an amplified colorimetric signal readout. The colorimetric sensor exhibited an ultralow detection limit (32 fM), showed excellent specificity and performed well in serum samples. The sensor was applied to detect miRNA-423-5p in clinical plasma samples from healthy individuals and HF patients, and the results revealed its good clinical application in HF diagnosis. Thus, the developed colorimetric sensor provides a convenient detection tool for early screening and diagnosis of HF, as well as for pathophysiological studies.
研究领域:
生物标志物检测
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纳米技术
·
分子诊断
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心血管疾病
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Yi Xiao,Pengcheng Huang,Fang-Ying Wu
α-Glucosidase, which directly involves in the metabolism of starch and glycogen and causes an increase in blood sugar level, is the major target enzyme for the precaution and therapy of type II diabetes. Based on the previous work, we adopted a post-synthetic modification method to encapsulate Tb 3+ into Ce-MOF nanozyme which owned mixed valence states. Tb@Ce-MOF displayed induced luminescence characteristic and exceptional oxidase-like activity that could oxidize colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue ox-TMB. α-Glucosidase can hydrolyze the substrate l -ascorbic acid-2- O -α- d -glucopyranosyl (AAG) to generate ascorbic acid (AA), which could increase the Ce 3+ /Ce 4+ redox valence mode in Tb@Ce-MOF, leading to the inhibition of the allochroic reaction of TMB and the decreased absorption of ox-TMB at 652 nm. The energy transfer (EnT) process from Ce 3+ to Tb 3+ will enhance due to the increased Ce 3+ /Ce 4+ mode in Tb@Ce-MOF, which will result in an enhanced fluorescence signal of Tb@Ce-MOF at 550 nm. But the addition of inhibitor acarbose will inhibit the above process. We have constructed a dual-mode detection platform of α-glucosidase and its inhibitor via colorimetric and fluorometric method. The linear range of α-glucosidase were 0.01–0.5 U/mL (colorimetric mode) and 0.8–1.5 U/mL (fluorometric mode), respectively, with a detection limit as low as 0.0018 U/mL. Furthermore, our approach was also successfully employed to the analysis of α-glucosidase in serum samples.
研究领域:
分析化学
·
生物化学
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材料科学
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糖尿病研究
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2019
·Vol. 11
·期 39
·5007-5016
·
IF 2.378
Xiaofei Qin,Can Chen,Shiyu Zhang,Jixin Zhu,Yongxiang Wang,Jinhua Liu
3,3′,5,5′-Tetramethylbenzidine (TMB) is one of the traditional enzyme substrates that has been widely used in the fields of artificial enzyme mimics and natural enzymes. However, the development of TMB-based probes for fluorescence and colorimetric dual models remains a great challenge. Herein, we developed a facile method to fabricate a colorimetric and fluorescent nanoswitch based on near-infrared fluorescent TMB dots (TMBDs) for the reversible recognition (CFRR) of Fe3+ and Cys. The proposed CFRR nanoswitch was based on an oxidation–reduction mechanism by which the TMBDs are oxidized by Fe3+ to form ensembles and Cys reduces the ensembles to TMBDs. The CFRR nanoswitch exhibited high sensitivity for the colorimetric and fluorescent recognition of Fe3+ with limits of detection (LODs) of 0.17 μM and 7.5 μM and of Cys with LODs of 0.1 μM and 1.5 μM, respectively. The response speed was very fast, within 5 min. Moreover, two output signals, namely near-infrared fluorescent and colorimetric, were exhibited in this CFRR sensor; the recognition was reversible and retained good stability after three cycles. It is noteworthy to mention that the CFRR nanoswitch performed successfully in urine, and molecular logic gate systems were readily established. Therefore, these investigations will shed light on the development of multi-mode output signals for nanoprobes.
研究领域:
分析化学
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纳米技术
·
生物传感器
·
材料科学
·
生物化学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Minyang Zhao,Xiaodong Lin,Xiao Zhou,Yujie Zhang,Haotian Wu,Yaqing Liu
Simple and efficient identification of multiple bacteria and sterilization in real time is of considerable significance for clinical diagnostics and quality control in food. Herein, a novel chemical-tongue sensor array with 3,3′,5,5′-tetramethylbenzidine (TMB) as a single probe was developed for bacterial identification and photothermal elimination. The synthesized bimetallic palladium/platinum nanoparticles (Pd/PtNPs) present excellent catalytic capability that can catalyze TMB into oxidized TMB (oxTMB) with four feature absorption peaks. Bacteria have different ability on inhibiting the reaction between TMB and Pd/PtNPs. With the absorbance intensity of oxTMB at the four feature peaks as readout, nine kinds of bacteria including two drug-resistant bacteria can be successfully distinguished via linear discriminant analysis. Remarkably, oxTMB exhibits excellent photothermal properties and can effectively kill bacteria in real time under near-infrared laser irradiation. The strategy of selecting TMB as a single probe simplifies the experimental operation and reduces the time cost. Furthermore, the developed sensing system was used to promote the wound healing process of MRSA-infected mice in vivo. The investigation provides a promising simple and efficient strategy for bacterial identification and sterilization with a universal platform, which has great potential application in clinical diagnosis and therapy.
研究领域:
临床诊断
·
食品质量控制
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纳米技术
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生物医学工程
·
光热治疗
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 11
·1-11
·
IF 6.408
Zhou Hang-Yu,Peng Li-Jing,Tian Tao,Zhang Wei-Yi,Chen Guo-Ying,Zhang Hao,Yang Feng-Qing
An effective, selective, and multicolor colorimetric assay for Cu 2+ detection based on the regulation of peroxidase-like nanozyme-mediated etching of gold nanorods (Au NRs) is proposed. Cu 2+ -creatinine complex is selected as the nanozyme that exhibits excellent peroxidase-like activity even in the case of low concentration of Cu 2+ , which can catalyze 3,3,5,5-tetramethylbenzidine (TMB) to produce oxidized TMB (TMB + ) in the presence of hydrogen peroxide, and TMB + is oxidized to generate TMB 2+ after adding H + , and the TMB 2+ can etch Au NRs. The determination of Cu 2+ is achieved based on the blue shift of the longitudinal localized surface plasmon resonance peak of Au NRs. Under the optimal conditions, the developed colorimetric assay exhibits high sensitivity for the detection of Cu 2+ (limit of detection is 0.034 μM) with a wide linear range of 0.05–4.0 μM ( R 2 = 0.987). The solution shows a rainbow-like color in response to the increase of Cu 2+ concentration, which can realize the semi-quantitative detection of Cu 2+ by naked eyes. In addition, the developed method exhibits excellent selectivity for Cu 2+ -detection. The established method was used for the determination of Cu 2+ in lake water, soil, and normal human serum with satisfactory recovery of spiked samples. Graphical abstract
研究领域:
分析化学
·
纳米技术
·
环境科学
·
生物化学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）