JOURNAL OF APPLIED POLYMER SCIENCE
·
2010
·Vol. 117
·期 1
·235-242
·
IF 1.203
Hongtao Liu,Weilin Xu,Sanping Zhao,Jingjing Huang,Hongjun Yang,Yunli Wang,Chenxi Ouyang
Biomedical polyurethane (PU) and silk fibroin have similar molecular architecture in their primary and aggregate structure, both of which have imido bonds and microphase separation, and they have been employed as scaffold materials for biomedical applications. As the featured peptide sequence of silkworm silk protein, GlyAlaGlyAla (GAGA) tetrapeptide was synthesized by using traditional liquid-phase peptide synthesis method with Boc-protected alanine and glycine as starting materials, and was transformed to its derivative with two end-amido groups. The derivative was incorporated into the backbone of the classical synthetic copolyurethane as a chain extender to form a silk-inspired polyurethane. Elemental analysis showed the nitrogen content in the silk-inspired PU was higher than that in the butanediol-extended PU but lower than the theoretical value, indicating the chain extender containing GAGA was partially incorporated into the PU chain. The results from 1 H-nuclear magnetic resonance, Fourier transform infrared spectra, Raman scattering spectra and pyrolysis-gas chromatography/mass spectrometry qualitatively identified the primary structure of the silk-inspired polyurethane. This concept and strategy may allow the fabrication of a new class of thermoplastic polyurethane elastomer to mimic the structure and properties of silk fibers if in fiber form, which combines traditional synthetic polymer chains with peptide sequences. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
研究领域:
材料科学
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生物医学工程
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DOI
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2013
·Vol. 130
·期 1
·631-637
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IF 1.395
Hongtao Liu,Jiangang Zhou,Xiuying Liu,Danying Zuo,Shaojin Gu,Weilin Xu
Biomedical polyurethane (BPU) and silk fibroin have similarly molecular architecture in their primary and aggregate structure, both of which have amide bonds and microphase separation, and they have been employed as scaffold materials for biomedical applications. Based on this, as the featured peptide sequence of silkworm silk fibroin, GlycineAlanineGlycineAlanine (GlyAlaGlyAla) tetrapeptide was synthesized by using traditional liquid-phase peptide synthesis method with Boc-protected glycine and alanine as starting materials, and was transformed to its derivative with two amine-terminated functional groups. The derivative was introduced as a chain extender into the backbone to form the hard segment of a silk-inspired PU with urea-linkage. Related measurements show that molecular weight of the synthesized silk-inspired PU ranged from 13,000 to 15,000. Fourier transform infrared (FTIR) absorption bands of Amides I and II are at 1651 cm −1 and 1534 cm −1 , and Raman absorption band of Amide III is at 1302 cm −1 . UV-Vis absorption peak of the silk-inspired PU is at 266 nm. This new concept and strategy may allow the fabrication of a new class of thermoplastic polyurethane elastomers to mimic the structure and properties of silk fibroin of silkworms and spiders. Information provided by this study may be used to better understand the correlation between the natural and man-made materials. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
研究领域:
生物医学材料
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高分子化学
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DOI
高影响因子
RSC Advances
·
2018
·Vol. 8
·期 54
·30887-30893
·
IF 2.936
Wen-Qiang Ding,Si-Yong Qin,Yin-Jia Cheng,Yi-Han Ma,Ai-Qing Zhang
Here we designed and constructed a tryptophan-phenylalanine-phenylalanine-tryptophan (WFFW) tetrapeptide, which generated photostable and tunable fluorescence emission signals from 340 nm to 500 nm. The WFFW tetrapeptide could self-assemble into a spherical nanostructure with enhanced fluorescence intensity. Driven by π–π stacking and hydrogen bond interaction, WFFW co-assembled with arginine-glycine-aspartic acid (RGD) modified WFFW to form a cancer-targeted fluorescent nanoprobe, which could selectively image the cancer cells.
研究领域:
生物化学
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医学影像
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纳米医学
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
·
2013
·Vol. 131
·期 10
·
IF 1.395
Hongtao Liu,Jiangang Zhou,Danying Zuo,Xiuying Liu,Shaojin Gu,Weilin Xu
Morphological, thermal, and mechanical features of electrosprayed and electrospun deposition of the silk-inspired polyurethane (PU) containing GlycineAlanineGlycineAlanine (GlyAlaGlyAla, the featured peptide sequence of silkworm silk fibroin) tetrapeptide, which was synthesized by the traditional liquid-phase peptide synthesis method and the classical two-step polymerization method using Boc-protected amino acids and diisocyanates as starting materials, were characterized. The results show that the synthesized silk-inspired PU dissolved in tetrahydrofuran (THF) can be easily electrosprayed or electrospun into the film form, although its molecular weight ranging from 13,000 to 15,000 is quite low. Elastomeric fibrous membranes with surface morphologies of “droplets,” “bead-on-string,” and “nonwoven fibers” have been obtained by electrospraying and electrospinning the silk-inspired PU/THF solution of varying concentrations. The thermograms confirm high thermostability of the silk-inspired PU between 350 and 400°C due to the polar peptide linkages. The tanδ peak of dynamic mechanical analysis curve corresponding to its glass transition temperatures is detected at −34.3°C. Its elongation at break is about 140–150%, and the breaking tensile strength ranges from 22 to 27 MPa, which is consistent with the data of other PUs containing l -alanine residue. Information provided by this study can be used to better understand the correlation between the natural and man-made silk polymers. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131 , 40245.
研究领域:
材料科学
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生物材料学
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产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2019
·
IF 2.298
Hong-Yun Zhu,Sheng-Gang Chen,Qilei Zhang,Dong-Qiang Lin,Shan-Jing Yao
Alternative antibody-binding affinity ligands are expected to support robust time- and cost-effective antibody purification with rapid improvement of upstream productivity and demand on downstream process optimization. Five new tetrapeptide biomimetic affinity ligands (FYRH, HWRH, YHRI, HYRF, and FHRA) were screened by modeling the molecular interaction mechanism between tetrapeptide ligands and the Fc fragment of IgG with molecular dynamics simulation and the flexible docking method, and the corresponding five resins were prepared in this work to evaluate their adsorption characteristics of human immunoglobulin G (hIgG). The results showed that the binding capacities to hIgG of the five resins showed a strong pH-dependency, which was high at pH 7.0–9.0, and extremely low at pH 5.0–6.0, providing a benefit choice to separate hIgG. The dynamic binding capacities (DBC) at 10% breakthrough of five resins could reach up to 28–37 mg/mLgel, and Ac-FYRH-4FF resin showed the best adsorption performance. The elution test results with Ac-FYRH-4FF resin indicated that hIgG could be eluted mildly at pH 5.0 with a 96.5% recovery, meaning that this resin can be considered as a powerful candidate of alternative biomimetic affinity chromatographic resin.
研究领域:
生物化学
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蛋白质纯化
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DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2016
·
IF 2.463
Rong-Zhu Wang,Dong-Qiang Lin,Wen-Ning Chu,Qi-Lei Zhang,Shan-Jing Yao
Peptide ligands have been developed as powerful alternatives to Protein A for antibody purification. In this study, a tetrapeptide library with two aromatic residues, one arginine and one aromatic/aliphatic residue (Aro-Aro-R-Aro/Ali) was constructed considering molecular interactions and applications. The library had 128 peptide ligands and was screened by flexible docking and molecular dynamics simulation. The results indicated that Ac-YFRH was the best ligand, and then was coupled onto an aminated argarose matrix. The results of isotherm adsorption showed that the binding capacity of human immunoglobulin G (IgG) was high at pH 7⿿8 and low at pH 4⿿5. The IgG adsorption also showed obvious salt-independence, and the addition of salt could reduce unspecific adsorption caused by electrostatic interaction. After the optimization of pH and salt addition, high IgG purity of 98.4% could be obtained with the recovery of 89.4% from BSA-containing feedstock. The purification of monoclonal antibody from CHO cell culture supernatant was tested, and the purity and recovery reached 98.0% and 79.5%, respectively. The results demonstrated that the new resin with the tetrapeptide YFRH ligand had high affinity and flexible operation window for IgG purification.
研究领域:
生物化学
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分子生物学
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产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 3.978
Yu-Ming Fang,Dong-Qiang Lin,Shan-Jing Yao
Short peptide resin has a great advantage in the separation and purification of antibodies due to its high selectivity and stable ligand binding ability. In this study, a peptide library containing 480 tetrapeptides was established with six critical residues (Phe, Tyr, Leu, Glu, Ile, Lys) of Protein A binding to Fc part of the antibody and one Histidine . Then the library was screened by LibDock and five tetrapeptide ligands with high LibDock scores were selected. Subsequently, the electrostatic interaction energies and van der Waals interaction energies between the screened ligands and the Fc-A fragment of IgG were further calculated by molecular dynamics simulation to research the binding ability between ligands and target proteins. The simulation results showed that YEHF was the optimal ligand with higher van der Waals interaction energies. Then, four tetrapeptides (YFLH, FYHI, FYKH and YEHF) were selected as ligands for preparing adsorption resins, and the resins were used to bind hIgG and BSA by static adsorption experiments. The results showed that Ac-YEHF-4FF resin had higher hIgG binding ability and lower BSA binding ability, also indicating that YEHF was the optimal screened ligand. Therefore, molecular simulation was verified as a well use technology for antibody separation and purification.
研究领域:
生物化学
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分子生物学
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DOI
高影响因子
ELECTROCHIMICA ACTA
·
2014
·
IF 4.086
Xiaobo Wang,Jing Ju,Juan Li,Jiying Li,Qiuhui Qian,Chun Mao,Jian Shen
It is highly desirable to develop simple and rapid method to detect cancer cells. In this case, a simple method for electrochemical detecting cell numbers based cytosensor was investigated. Thioctic acid (TA) monolayer, assembled on a high conductive Au/ITO substrate to stable immobilized of Arg-Gly-Asp-Ser tetrapeptide (RGDS) by chemical bonding, was first used for construction of electrochemical cytosensor. Moreover the cytotoxicity assay also showed no cytotoxicity of TA modified electrode . The proposed cytosensor showed good performance for detection of cancer cells with a broad detection range of 3.0 × 10 2 to 1.0 × 10 7 cells/mL, and a low detection limit of 300 cells/mL. The results were obtained that can be attributed to the combination of the advantages of high conductive and biocompatible property of the modified electrode we suggested. This work demonstrated a simple strategy to prepare an electrochemical cytosensor with the property of low cost, good biocompatible, broad detection range, low detection limit, good repeatability which may have potential large-scale application for improving cancer diagnosis and treatment.
研究领域:
生物医学工程
·
癌症诊断
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2022
·
IF 3.318
Yu-Ming Fang,Qi-Lei Zhang,Dong-Qiang Lin,Shan-Jing Yao
Short peptide biomimetic chromatography technology as a developing protein separation technology has huge potential for antibody purification. In this study, four tetrapeptide ligands (Ac-FYKH, Ac-YEHF, Ac-YFLH and Ac-FYHI) with high potential binding ability to antibody were selected for the optimal ligand to antibody purification. The results showed that Ac-YEHF-4FF resin had higher binding capacity and selectivity for hIgG among the four resins. And at pH 7.0 and 0.3 ml/min, the highest Q 10%-hIgG of Ac-YEHF-4FF resin was 26.2 mg/ml resin while its Q 10%-BSA was just 2.2 mg/ml resin. Further, Ac-YEHF-4FF resin was used to purify protein mixtures. By binding at pH 7.0 and being eluted at pH 5.0 and pH 4.0, Ac-YEHF-4FF resin was well used to separate hIgG from BSA containing feedstock, HSA containing feedstock and human serum with the purity and yield both more than 95 %. And the screened resin could also separate mAb from CHO cell culture supernatant with purity 94.3 % and yield 97.5 %. The adsorption and separation results of Ac-YEHF-4FF resin indicated that the goal of getting the efficacy of critical residues from protein A to biomimetic its structure and function could be achieved, which had great significance to the establishment and improvement of tetrapeptide biomimetic chromatography, and also provided a new method for the field of antibody separation and purification.
研究领域:
生物化学
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蛋白质工程
·
分离技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2018
·
IF 2.196
Yu-Ming Fang,Dong-Qiang Lin,Shan-Jing Yao
Poly(ethylenimine) (PEI) as a long flexible chain has been well used in protein chromatography with the advantages of high spatial utilization and “chain delivery” effect. In this work, two resins, i.e., poly(ethylenimine)-4FF resin (PEI-4FF resin) and Ac-YFRH-PEI-4FF resin by grafting tetrapeptide to PEI-4FF resin, were prepared for the protein adsorption and chromatographic separation. The pH-dependent adsorption properties of PEI-4FF resin and Ac-YFRH-PEI-4FF resin were discovered by getting the data of adsorption capacities of bovine serum albumin (BSA) and immunoglobulin G (IgG) under different pH conditions and buffer systems. The results showed high binding capacities of BSA on PEI-4FF resins across a range of pH values (5.0–8.9) with almost very little adsorption of IgG over a pH range from 5.0 to 8.0, indicating that IgG could be separated by flow-through method using PEI-4FF resin. Since PEI has the advantage of having many flexible branched chains and Ac-YFRH (Ac-Try-Phe-Arg-His) as a peptide ligand has the advantages of comparatively high stability and low immunogenicity for antibody adsorption, Ac-YFRH-PEI-4FF resins were prepared by using PEI as the spacer arm and Ac-YFRH as the peptide ligand. The isotherm adsorption of the Ac-YFRH-PEI-4FF resin showed that the maximum binding capacity of IgG (qm = 123.6 mg/g resin, Kd = 0.10 mg/mL) was far more than that of BSA (qm = 62.0 mg/g resin, Kd = 0.25 mg/mL) at pH 8.0. Meanwhile, this tetrapeptide resin also showed high binding capacity of BSA (qm = 131.0 mg/g resin, Kd = 0.05 mg/mL) and low binding capacity of IgG (qm = 2.0 mg/g resin, Kd = 0.01 mg/mL) at pH 5.0, indicating that the separation of BSA and IgG could be achieved with the big differences in adsorption behaviors.
研究领域:
生物化学
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分离科学
·
蛋白质组学
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产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2019
·
IF 3.858
Yu-Ming Fang,Sheng-Gang Chen,Dong-Qiang Lin,Shan-Jing Yao
Biomimetic affinity chromatography with short peptide ligands is a developing technology, which has great potential for antibody separation and purification. In this study, a tetrapeptide library with critical residues of natural ligands to hIgG was constructed and a novel tetrapeptide ligand (Ac-FYHE) with high LibDock scores was selected by molecular docking. Then, Ac-FYHE ligand was linked to agarose bead to prepare a new chromatography resin. The properties of antibody adsorption were measured and evaluated by static/dynamic adsorption. It was found that the resin with ligand Ac-FYHE has high binding capacity and selectivity for hIgG. The results showed the Q m-hIgG of Ac-FYHE-4FF resin was 87.9 mg/g resin while the Q m-BSA of this resin was only 16.5 mg/g resin at pH 7.0. Moreover, at pH 7.0, Q 10% of Ac-FYHE-4FF resin was 24.1 mg/mL for hIgG but just 2.1 mg/mL for BSA, which presented high selectivity of the screened resin at pH 7.0. Subsequently, the adsorption and separation properties of the Ac-FYHE-4FF resin were further investigated. As a result, with the addition of 0.5 M NaCl , Q m decreased by less than 20% but Q m decreased by 70% with the addition of 50% (v/v) ethylene glycol , which indicated that hydrophobic interaction would be the driving force for the binding between resin and hIgG. Besides, pH 7.5 and pH 4.5 could be the optimal loading and elution condition for hIgG, respectively. Finally, the Ac-FYHE-4FF resin was applied to separate mAb or/and hIgG from BSA containing feedstock, CHO cell culture supernatant and human serum, and the purity and recovery were both more than 90% with only one-step separation. The results indicate that the Ac-FYHE-4FF resin developed in this work would be promising for antibody separation and purification.
研究领域:
生物化学
·
生物工程
·
制药工程
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DOI
PubMed
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2016
·Vol. 34
·期 6
·609-616
·
IF 1.872
Wei Zhang,Wenjun Zhu,Ruiyu He,Shuo Fang,Yemin Zhang,Chen Yao,Muhammad Ismail,Xinsong Li
Chlorambucil is a classic nitrogen mustard drug that has been used in the treatment of cancers. It may induce neutropenia, thrombocytopenia and other side effects because of its short lifetime and off-target effect. In this report, chlorambucil-tetrapeptide (AAAK, A 3 K) conjugate vesicles were developed to improve the stability and bioactivity of chlorambucil. First of all, chlorambucil-A 3 K conjugate was synthesized by solid phase synthesis strategy. Secondly, the chlorambucil-A 3 K conjugate was assembled and characterized by critical aggregation concentration, circular dichroism, dynamic light scattering and transmission electron microscopy. The results indicated that the chlorambucil-A 3 K conjugate can be assembled to form spherical vesicles with an average diameter of 390.5 nm, and high drug loading about 47.1% is reached. Surprisingly, the preliminary biological evaluation of the chlorambucil-A 3 K conjugate vesicles revealed the best in vitro anticancer activity against HeLa, HepG-2 and MCF-7 cell lines compared with chlorambucil and chlorambucil-A 3 K conjugate free drugs. Furthermore, conjugate vesicles showed excellent in vivo antitumoral activity. It can be partly attributed to their vesicular structure which isolates chlorambucil active moiety from aqueous solution to retard degradation before killing cancer cells. Therefore, chlorambucil-peptide (A 3 K) conjugate vesicles may be an alternative delivery system of chlorambucil.
研究领域:
药学
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癌症研究
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纳米技术
·
药物递送系统
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）