FOOD CHEMISTRY
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2024
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IF 8.5
Yu Zou,Wanshan Wang,Fengyin Duan,Wen Yun,Hong Chen,Yiping Li,Lizhu Yang
An ultrasensitive fluorescence detection strategy of chloramphenicol (CAP) was developed based on product catalysis of tetrahedral DNA framework (TDF) and fluorescent quenching of MIL-101(Fe). The product was used to catalyze the reaction. As the concentration of catalyst increased, the reaction time was significantly shorted to 21 min which was much shorter than other isothermal amplification technologies. Moreover, the multiple fluorophores of TDF and high efficient quenching ability of MIL-101(Fe) provided better performance with a linear range for CAP detecting from 1.6 pM to 80 pM and the limit of detection (LOD) as low as 0.67 pM. In addition, it also demonstrated good specificity and resistance to interference from other related antibiotics. Importantly, this strategy exhibited satisfactory relative standard deviation and recovery results for practical application, exhibiting a favorable application prospect in CAP analysis.
研究领域:
生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-15
·
IF 14.7
Zhou Zhixin,Han Bing,Wang Yu,Lin Nina,Zhou Zhongqiu,Zhang Yuan,Bai Ying,Shen Ling,Shen Yanfei,Zhang Yuanjian,Yao Honghong
While circular RNAs (circRNAs) exhibit lower abundance compared to corresponding linear RNAs, they demonstrate potent biological functions. Nevertheless, challenges arise from the low concentration and distinctive structural features of circRNAs, rendering existing methods operationally intricate and less sensitive. Here, we engineer an intelligent tetrahedral DNA framework (TDF) possessing precise spatial pattern-recognition properties with exceptional sensing speed and sensitivity for circRNAs. The signal output of TDF sensor occurs only when multivalent spatial pattern-recognition of a circRNA in unamplified samples. Using this sensor, we visualize the real-time response of endogenous circRNA expression in vitro neuronal cells and in vivo brain between pre-stroke and post-stroke male mice, identify the patients with acute ischemic stroke in clinical samples, as well as track the delivery of circRNA in photochromic stroked animal model. Thus, the TDF sensor provides a fast and sensitive tool for the detection of circRNA abundance in both physiological and pathophysiological conditions.
研究领域:
分子生物学
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生物医学工程
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神经科学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2024
·Vol. 22
·期 1
·1-14
·
IF 10.6
Jiang Bowen,Zhang Tenghua,Liu Silan,Sheng Yan,Hu Jiaming
Background Exosomes are nanoscale extracellular vesicles (30–160 nm) with endosome origin secreted by almost all types of cells, which are considered to be messengers of intercellular communication. Cancerous exosomes serve as a rich source of biomarkers for monitoring changes in cancer-related physiological status, because they carry a large number of biological macromolecules derived from parental tumors. The ultrasensitive quantification of trace amounts of cancerous exosomes is highly valuable for non-invasive early cancer diagnosis, yet it remains challenging. Herein, we developed an aptamer-carrying tetrahedral DNA (Apt-TDNA) microelectrode sensor, assisted by a polydopamine (PDA) coating with semiconducting properties, for the ultrasensitive electrochemical detection of cancer-derived exosomes. Results The stable rigid structure and orientation of Apt-TDNA ensured efficient capture of suspended exosomes. Without PDA coating signal amplification strategy, the sensor has a linear working range of 10 2 –10 7 particles mL −1 , with LOD of ~ 69 exosomes and ~ 42 exosomes for EIS and DPV, respectively. With PDA coating, the electrochemical signal of the microelectrode is further amplified, achieving single particle level sensitivity (~ 14 exosomes by EIS and ~ 6 exosomes by DPV). Conclusions The proposed PDA-assisted Apt-TDNA microelectrode sensor, which integrates efficient exosome capture, sensitive electrochemical signal feedback with PDA coating signal amplification, provides a new avenue for the development of simple and sensitive electrochemical sensing techniques in non-invasive cancer diagnosis and monitoring treatment response. Graphical
研究领域:
生物医学工程
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分子生物学
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肿瘤学
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分析化学
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纳米技术
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电化学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 2
·1-8
·
IF 5.3
Ou Dan,Yan Huixian,Chen Zuanguang
Based on the electrochemical impedance method, a marker-free biosensor with aptamer as a biometric element was developed for the determination of doxorubicin (DOX). By combining aptamer with rigid tetrahedral DNA nanostructures (TDNs) and fixing them on the surface of gold electrode (GE) as biometric elements, the density and directivity of surface nanoprobes improved, and DOX was captured with high sensitivity and specificity. DOX was captured by immobilized aptamers on the GE, which inhibited electron transfer between the GE and [Fe(CN)6] 3− / 4− in solution, resulting in a change in electrochemical impedance. When the DOX concentration was between 10.0 and 100.0 nM, the aptasensor showed a linear relationship with charge transfer resistance, the relative standard deviation (RSD) ranged from 3.6 to 5.9%, and the detection limit (LOD) was 3.0 nM. This technique offered a successful performance for the determination of the target analyte in serum samples with recovery in the range 97.0 to 99.6% and RSD ranged from 4.8 to 6.5%. This method displayed the advantages of fast response speed, good selectivity, and simple sensor structure and showed potential application in therapeutic drug monitoring. Graphical Abstract
研究领域:
生物传感器技术
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药物分析
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纳米生物学
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
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IF 13.3
Mengxin Zhang,Yingyu Zhang,Xianwei Zhang,Qionglin Wang,Kangbo Liu,Muchun Yu,Lifeng Li,Zhidan Yu,Huan Zhao,Weyland Cheng,Wancun Zhang
Specific imaging of tumor cells with high sensitivity is urgently needed for evaluating the risk of tumor metastasis and progression. However, traditional tumor cell imaging strategies are still restricted by limited tumor specificity and sensitivity due to off-tumor signal leakage and a lower content of the targets. Therefore, a self-powered and self-recycled strategy based on molecular beacon (MB) hybridized on tetrahedral framework DNA (E-MB-tFNA) using the endogenous enzyme, apurinic/apyrimidinic endonuclease 1 (APE1), was rationally developed for tumor cell-specific molecular imaging with superior spatial specificity and improved sensitivity. The apurinic/apyrimidinic site (AP site) can be specifically recognized and cleaved by APE1, and was modified on the stem and loop region of E-MB-tFNA. The resultant E-MB-tFNA can be specifically recognized and cleaved by APE1 in the cytoplasm, which is overexpressed in tumor cells compared to normal cells. In particular, once APE1 cleaves the AP site on the stem region (double-stranded DNA) of E-MB-tFNA, the self-powered and self-recycled sequences modified on the stem region of E-MB-tFNA is freed to hybridize with the loop region of E-MB-tFNA, prompting APE1 to further hydrolyze the E-MB-tFNA loop region and generate fluorescence signals. In addition, the self-powered and self-recycled sequences can be hybridized with other E-MB-tFNA to continuously and cyclically amplify the fluorescence signal. The experimental results of this study showed that the E-MB-tFNA has the capability to monitor drug resistance and achieve guided precision surgical excision in vivo with superior spatial specificity and improved sensitivity. In particular, E-MB-tFNA can realize the risk classification of neuroblastoma patients in clinical plasma.
研究领域:
生物医学工程
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分子生物学
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肿瘤学
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产品关联
DOI
高影响因子
Theranostics
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2018
·
IF 8.537
Shulian Bai,Bangtian Xu,Yongcan Guo,Juhui Qiu,Wen Yu,Guoming Xie
While detection of microRNA with or without signal amplification is highly informative, nanosensors with high specificity for cell-specific RNA detection are rare. Methods: In this study, a tetrahedral DNA nanostructure (TDN) with a specific function was combined with gold nanoparticles (Au-NP) possessing fluorescence quenching effects and a large surface area to fabricate a fluorescence resonance energy transfer based nanosensor (Au-TDNN). The presence of miR-21 (target) can separate the fluorescent dye-labeled detection probe on Au-TDNNs from Au-NPs, which separates the donor and acceptor, thus inducing an intensive fluorescence signal. High specificity for discerning point mutation targets was achieved by rationally designing the nucleic acid strand displacement reaction to occur spontaneously with ΔG 0 ≈ 0 based on thermodynamic parameters; under this condition, slight thermodynamic changes caused by base mismatch exert significant effects on hybridization yield. Results: Chemically synthesized DNA of three single-base-changed analogues of target, let-7d, and miR-200b were tested. A discrimination factor (DF) of 15.4 was produced by the expected detection probe on Au-NPs for proximal single-base mismatch. As the control group, the DF produced by an ordinary detection probe on Au-NPs only reached 2.4. The feasibility of the proposed strategy was also confirmed using hepatocyte cancer cells (HepG2). Conclusion: This improved nanosensor opens a new avenue for the specific and easy detection of microRNA in live cells.
研究领域:
分子生物学
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纳米医学
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生物传感技术
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 5
·1-10
·
IF 5.3
Han Jiaqi,Zhu Nianxin,Sha Jiahao,Cai Jinyan,Cao Hui,Ye Tai,Hao Liling,Xu Fei
A novel electrochemical biosensor for the detection of lead ions (Pb 2+ ) with improved specificity and sensitivity was developed. The sensor design incorporated molecular imprinting technology, where chitosan was polymerized on the electrode surface to form a lead-specific cavity structure, thereby enhancing selectivity in complex sample matrices. Meanwhile, the tetrahedral DNA nanostructure was employed as the recognition probe to mitigate the entanglement issues commonly associated with single-stranded DNA, thus improving the sensitivity of the detection. The developed sensor exhibited a linear dynamic range from 0.050 to 2.000 μg/mL, with a limit of detection (LOD) of 0.0034 μg/mL. The aptasensor’s efficacy was verified through the analysis of aquatic samples, demonstrating a high degree of reliability comparable to that of inductively coupled plasma mass spectrometry (ICP-MS). Graphical
研究领域:
生物传感
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环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
BIOMEDICAL AND ENVIRONMENTAL SCIENCES
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2023
·
IF 3.5
Hao WU,Jiang Shan XU,Yan Hong LI,Xing Han WU,Wei HU,Meng Die LIU,Qiang SUN,Bin GUO
Objective To develop an effective treatment strategy to simultaneously avoid fatal adverse effects in the treatment of oral cancer, combination therapy has been explored because of its multiple functions. This work aims to develop a novel type of gold-nanorod-based nanomaterials decorated with tetrahedral DNA nanostructures (TDN) carrying antitumor drugs, namely, [email protected] nanocomposites. Methods In the designed structure, TDN, with a three-dimensional geometry composed of DNA strands, can provide GC base pairs for binding with the anticancer drug doxorubicin (DOX). The photothermal heating properties, biocompatibility properties, and antitumor performance of obtained [email protected] nanocomposites were investigated to assess their application potential in tumor treatment. Results Systematic studies have shown that the obtained [email protected] nanocomposites have high photothermal conversion under the illumination of an 808-nm infrared laser, leading to effective antitumor applications. In addition, the cell viability study shows that [email protected] nanocomposites have good biocompatibility. In vitro studies based on A375 cells show that the [email protected] nanocomposites can effectively eliminate cancer cells because of the combination of photothermal therapy induced by GNRS and chemotherapy induced by TDN-carrying DOX. The result shows that the obtained [email protected] nanocomposites have efficient cellular uptake and lysosome escape ability, together with their nuclear uptake behavior, which results in a significant antitumor effect. Conclusion This work has demonstrated a potential nanoplatform for anticancer applications.
研究领域:
纳米技术
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癌症研究
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材料科学
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生物医学工程
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2021
·
IF 6.558
Guoyan Zhao,Yongmei Liu,Jie Du,Huizi Zhang,Hanqing Feng,Xiaoquan Lu
Extracellular ATP (eATP) is an important biological signal transduction molecule. Although a variety of detection methods have been extensively used in ATP sensing and analysis, accurate detection of eATP remains difficult due to its extremely low concentration and spatiotemporal distribution. Here, an eATP measurement strategy based on tetrahedral DNA (T-DNA)-modified electrode sensing platform and hybridization chain reaction (HCR) combined with G-quadruplex/Hemin (G4/Hemin) DNAzyme dual signal amplification is proposed. In this strategy, ATP aptamer and RNA-cleaving DNAzyme were combined to form a split aptazyme . In the presence of ATP, this aptazyme hydrolyzes the cleaving substrate strand with high selectivity , releasing cleaved ssDNA , which are captured by the T-DNA assembled on the electrode surface, triggering an HCR on the electrode surface to form numerous linker sequences of the HCR dsDNA product. When [email protected] (G4) spherical nucleic acid enzymes (SNAzymes) with other linkers are used as nanocatalyst tags, they are captured by HCR dsDNA through sticky linkers present on the electrode surface. An amplified electrochemical redox current signal is generated through SNAzyme-mediated catalysis of H 2 O 2 , enabling easy detection of picomole amounts of ATP. Using this strategy, eATP levels released by tobacco suspension cells were accurately measured and the distribution and concentration of eATP released on the surface of an Arabidopsis leaf was determined.
研究领域:
植物学
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分子生物学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Jiangle Yi,Dong Tian,Changli Zhou,Fangquan Xia,Chuannan Luo
A novel electrochemiluminescence (ECL) aptasensor based on tetrahedral DNA nanostructures (TDN) and CdTe QDs was fabricated for the detection of acetamiprid residues. Three TDNs with different sizes were successfully designed and synthesized. The unique and rigid tetrahedral structure of TDN can ensure that the biological probe always maintains the orientation perpendicular to the sensing interface, greatly reducing the background noise and effectively improving the signal-to-noise ratio. It has great significance to improve the order of the probe on the sensing interface. The ECL signals of TDN with different sizes were compared with the conventional aptasensors (c-DNA) modified by single-stranded DNA (ssDNA). The results displayed that TDN can effectively amplify the sensor’s ECL signal, the size of TDN was negatively correlated with the amplification coefficient and hybridization time of aptamer. Compared various parameters of the c-DNA sensor and TDN 13 sensor which with the highest signal. The linear range of the TDN 13 sensor is 0.100 pM∼100 nM, the detection limit is 34.0 fM. The linear range of the c-DNA sensor is 1.00 pM∼10.0 nM, the detection limit is 178 fM. TDN 13 sensor was applied to measure acetamiprid residues in Chinese cabbage samples with satisfactory results.
研究领域:
生物化学
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分析化学
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纳米生物学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2025
·
IF 5.6
Chuang Zhang,Xinhua Zhang,Jian Zhang,Mian Peng,Xiaowen Dou
Circulating tumor cells (CTCs), though rare and highly heterogeneous, are closely associated with tumor initiation, progression, and prognosis. Their low abundance, structural fragility, phenotypic diversity, and size variability make efficient enrichment particularly challenging. To address these limitations, we developed a novel CTC isolation platform that combines tetrahedral DNA nanostructure-based trivalent aptamer magnetic nanospheres (TDF-Cocktail-MNP) with a magnetic microfluidic separation device. This system enhances the capture of viable, phenotypically diverse CTCs and supports reliable downstream analysis. The TDF structure presents three aptamers: EpCAM targets epithelial CTCs, Vimentin captures mesenchymal types, and EGFR binds to cells overexpressing EGFR—a key marker in triple-negative breast cancer. This multi-target approach enables efficient isolation of heterogeneous CTC populations from whole blood samples. Captured CTCs are enriched using the IsoFlux System, which reduces mechanical stress and preserves cell viability by avoiding repeated magnetic manipulation. After nuclease-mediated removal of the DNA scaffold, cells are classified and quantified through immunocytochemistry. Validated with both spiked and clinical breast cancer samples, this platform shows strong potential for liquid biopsy applications, offering robust CTC detection and valuable insights for personalized cancer diagnostics and treatment.
研究领域:
肿瘤学
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分子生物学
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纳米医学
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转化医学
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生物材料
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液体活检
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微流控技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 4
·915-922
·
IF 3.637
Wu Zhi-Wei,Xie Xing-Chen,Guo Hong-Ran,Xia Huan,Huang Ke-Jing
A tetrahedral DNA probe can effectively overcome the steric effects of a single-stranded probe to obtain well-controlled density and minimize nonspecific adsorption. Herein, a highly sensitive electrochemical biosensor is fabricated for determination of protein using a tetrahedral DNA probe and rolling circle amplification (RCA). N- and P-co-doped graphene (NP-rGO) is prepared, and AuNPs are then electrodeposited on it for DNA probe immobilization. Benefitting from the synergistic effects of the excellent electrical conductivity of NP-rGO, the stability of the tetrahedral DNA probe and the signal amplification of RCA, the biosensor achieves a low limit of 3.53 × 10 −14 M for thrombin and a wide linear range from 1 × 10 −13 to 1 × 10 −7 M. This study provides a sensitive and effective method for the detection of protein in peripheral biofluids, and paves the way for future clinical diagnostics and treatment of disease.
Graphical abstract
研究领域:
生物化学
·
分析化学
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生物传感器
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分子诊断
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）