The anti-washout ability of calcium phosphate cement (CPC) determines its effectiveness in clinical application. In the current research, the common method for improving the anti-washout ability of CPC is to add anti-washout polymer agents. Sodium polyacrylate powder is an excellent anti-washout agent but when bonded with CPC it basically degrades the anti-washout performance of CPC after γ-ray irradiation, and is widely used in the sterilization process of CPC products. Therefore, we propose a method for the preparation of a sodium polyacrylate solution through irradiation polymerization as curing solution for CPC. This method first uses γ-ray irradiation sterilization to improve the anti-washout ability of CPC directly. It not only avoids the adverse effects of γ-rays on anti-washout agents, but also the CPC blended using this sodium polyacrylate solution had good biological properties and injectability. It provides a new method for promoting the anti-washout properties of calcium phosphate cement, which is of great significance for expanding the clinical application of CPC.
Kun Liu,Lin Li,Yizhi Li,Yiting Luo,Zhaoyu Zhang,Wei Wen,Shan Ding,Yadong Huang,Mingxian Liu,Changren Zhou,Binghong Luo
Osteoid plays a crucial role in directing cell behavior and osteogenesis through its unique characteristics, including viscoelasticity and liquid crystal (LC) state. Thus, integrating osteoid-like features into 3D printing scaffolds proves to be a promising approach for personalized bone repair. Despite extensive research on viscoelasticity, the role of LC state in bone repair has been largely overlooked due to the scarcity of suitable LC materials. Moreover, the intricate interplay between LC state and viscoelasticity in osteogenesis remains poorly understood. Here, we developed innovative hydrogel scaffolds with osteoid-like LC state and viscoelasticity using digital light processing with a custom LC ink. By utilizing these LC scaffolds as 3D research models, we discovered that LC state mediates high protein clustering to expose accessible RGD motifs to trigger cell-protein interactions and osteogenic differentiation, while viscoelasticity operates via mechanotransduction pathways. Additionally, our investigation revealed a synergistic effect between LC state and viscoelasticity, amplifying cell-protein interactions and osteogenic mechanotransduction processes. Furthermore, the interesting mechanochromic response observed in the LC hydrogel scaffolds suggests their potential application in mechanosensing. Our findings shed light on the mechanisms and synergistic effects of LC state and viscoelasticity in osteoid on osteogenesis, offering valuable insights for the biomimetic design of bone repair scaffolds.
We develop a kind of photocurable liquid crystal hydrogels with bone extracellular matrix (ECM)-like liquid crystal state and viscoelasticity , as well as different chargeability. First, positively charged chitin whiskers (CHWs) and negatively charged maleic anhydride chitin whiskers (mCHWs) were prepared, which further self-assemble to form chiral nematic liquid crystals under ultrasonic treatment, respectively. Subsequently, poly (ethylene glycol) diacrylate (PEGDA) and photo initiator were added, and then two kinds of liquid crystal hydrogels with bone ECM-like viscoelasticity and different chargeability were prepared under ultraviolet (UV) irradiation. Benefiting from the bone ECM-like liquid crystal state and viscoelasticity, the prepared liquid crystal hydrogels exhibit remarkable cell affinity and osteogenic ability. Moreover, the liquid crystal hydrogel with negatively charged mCHWs is more favorable for cell adhesion , spreading and osteogenic differentiation than that with positively charged CHWs. This work provides a promising strategy to prepare the hydrogels with bone ECM-like liquid crystal properties and viscoelasticity for bone repair .
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