JOURNAL OF FOOD SCIENCE
·
2024
·
IF 3.2
Desy Anggraini Soetanto,Fengnan Li,Isaac Duah Boateng,Xiao-Ming Yang
Ginkgo biloba leaves (GBLs) contain high phytoconstituents, but ginkgolic acids (GAs, the main toxic compound in GBLs) have limited its applications. Processing Ginkgo biloba dark tea (GBDT) using fixation technology could decrease the toxic compounds; retain flavonoids, ginkgolides, and bilobalide; and improve the product quality. For the first time, various thermal fixations (hot air fixation [HAF], iron pot fixation [IPF], and boiled water fixation [BWF]) followed by rolling, fermentation, and drying were applied to produce GBDT. A comprehensive analysis of the toxicants (GAs), main bioactive compounds (ginkgolides and bilobalide, flavonoids, antioxidants, and phenolic profiles), and product qualities (moisture content, reducing sugar [RS], free amino acids [FAAs], enzyme activity, color properties, antioxidant capacity, etc.) were evaluated. The results revealed that thermal fixations BWF and HAF significantly reduced the GA contents (41.1%–34.6%). Most terpene lactones showed significant differences in control, IPF, and HAF. The HAF had lower total flavonoid content (TFC) than BWF and IPF. The control group (unfixated) had the highest toxic components (GA), terpene trilactones, and TFC compared with various fixations. Adding different fixations to rolling, fermentation, and drying had various impacts on GBDT, and principal component analysis supported the results. Among four thermal fixations, HAF yielded the best results in RS, FAA, total phenolic content, and antioxidant activities, while IPF had the highest TFC. BWF had the lowest content for GA. In conclusion, HAF (6) was chosen as the best technique for producing GBDT since it preserved GBDT's bioactive components while lowering its toxic components.
研究领域:
食品科学
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药理学
·
植物科学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 11
·8458-8468
·
IF 3.201
Jiayi Song,Ting Lei,Ye Yang,Nan Wu,Ping Su,Yi Yang
The major barriers to the use of enzymes in practical applications are their insufficient stability and easy inactivation under processing conditions. In this work, we developed a promising platform that allows for the simple and effective immobilization of enzymes on hydrophilic polydopamine (PDA) modified magnetic nanoparticles through DNA directed immobilization. Taking α-chymotrypsin (ChT) as an example, the well-designed immobilized enzyme in this study exhibited excellent kinetic performance, and the apparent Vmax and catalytic efficiency (kcat/Km) values of the DNA immobilized enzyme were 15.77 mM min−1 mg protein−1 and 4.05 s−1 mM−1, which were more than 6.0- and 5.9-fold enhanced compared to that of the free enzyme, respectively. The DNA immobilized enzyme exhibited promising thermal stability, which could preserve promising enzymatic activities of about 83% after 60 min incubation at 60 °C. The DNA immobilized enzyme also exhibited excellent long-term storage and incubation stability, which could preserve more than 89% of the initial activity at 4 °C for 35 days and more than 77% after 47 h of incubation, respectively. In addition, the DNA immobilized ChT exhibited excellent reusability, which showed a high degree of activity (more than 84%) after 10 cycles. Notably, the enzyme immobilization procedure exhibited high reversibility and reproducibility, and the magnetic nanoparticle surfaces were successfully regenerated and cycled through DNA strand displacement reactions for subsequent enzyme immobilization, which could retrieve more than 98% of the enzymatic activity. A significantly improved protein digestion efficiency was achieved with this immobilized ChT within 10 min, and the obtained sequence coverages were more than 1.3-fold higher when compared to that obtained by conventional in-solution digestion for 12 h. Therefore, this work exhibits a promising alternative platform for the efficient immobilization of industrially important enzymes and their broad applications.
研究领域:
生物化学
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纳米生物学
·
酶工程
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产品关联
DOI
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
·
2018
·
IF 3.226
Ye Yang,Siming Wang,Zixin Zhou,Ruiqi Zhang,Hao Shen,Jiayi Song,Ping Su,Yi Yang
The development of novel approaches for preparing the immobilized enzyme reactors with improved reusability and catalytic performance has attracted tremendous interest because of the extensive applications and the high cost of free enzymes. Herein, we describe a mild and versatile method for the preparation of immobilized enzyme reactors by anchoring trypsin on polydopamine (PDA) functionalized magnetic nanoparticles (MNPs) through DNA directed immobilization (DDI). This reactor exhibited prominent reusability and enhanced catalytic efficiency. The specificity constant (k cat /K m , where k cat is the catalytic rate constant) of the reactor was 1.82 s −1 μM −1 , which was approximately 4.7-fold higher than free trypsin. Notably, the immobilized trypsin reactors maintained more than 55% of their initial activity after 70 cycles (in pH 9.0 PBS, 37 °C). The improved activity and reusability were dually benefited from the rigidity and stability of DNA linkers as well as the excellent biocompatibility and cushioning effects of PDA. Given the excellent reusability and high catalytic efficiency of this immobilized trypsin reactors, we believe that this study paves the way for preparing low-cost enzyme reactors for a wide range of applications in biocatalysis, biological diagnosis, and biotechnology.
研究领域:
生物催化
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生物技术
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生物医学工程
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产品关联
DOI
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2022
·Vol. 45
·期 12
·1955-1966
·
IF 3.434
Liu Runtang,Wang Silin,Han Mengyao,Zhang Wei,Xu Huajin,Hu Yi
In this study, an amino-functionalized ionic liquid-modified magnetic chitosan (MACS-NIL) containing 2,2-diamine-di-3-ethylbenzothiazolin-6-sulfonic acid (ABTS) was used as a carrier, and dialdehyde starch (DAS) was used as a cross-linking agent to covalently immobilize laccase (MACS-NIL-DAS-lac), which realized the co-immobilization of laccase and ABTS. The carrier was characterized by Fourier infrared transform spectroscopy, scanning electron microscopy, thermogravimetric analysis, X-ray diffraction analysis, electron paramagnetic resonance, etc. The immobilization efficiency and activity retention of MACS-NIL-DAS-lac could reach 76.7% and 69.8%, respectively. At the same time, its pH stability, thermal stability, and storage stability had been significantly improved. In the organic pollutant removal performance test, the removal rate of 2,4-dichlorophenol (10 mg/L) by MACS-NIL-DAS-lac (1 U) could reach 100% within 6 h, and the removal efficiency could still reach 88.6% after six catalytic runs. In addition, MACS-NIL-DAS-lac also showed excellent degradation ability for other conventional phenolic pollutants and polycyclic aromatic hydrocarbons. The research results showed that MACS-NIL-DAS fabricated by the combination inorganic material, organic biomacromolecules, ionic liquid, and electron mediator could be used as a novel carrier for laccase immobilization and the immobilized laccase showed excellent removal efficiency for organic pollutants.
研究领域:
生物技术
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环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
·
2015
·
IF 2.128
Xiaoyao Fei,Shaoyun Chen,Chunjie Huang,Dai Liu,Yongchun Zhang
Bovine carbonic anhydrase (BCA) has been immobilized on glycidoxypropyl-functionalized SBA-15 (GFS) for biomimetic carbonation reaction. GFS was characterized by N 2 adsorption–desorption, Fourier transform infrared analysis, 13 C and 29 Si CP MAS NMR spectroscopy. The immobilization time and material dose were optimized. The influences of pH value and temperature on the activity of free and immobilized BCA, storage stability and reusability of immobilized BCA were investigated using a para-nitrophenyl acetate (p-NPA) assay. The kinetic parameters K m and K cat / K m for free and immobilized BCA were found to be 2.4 mM and 896.4 M −1 s −1 , and 3.1 mM and 757.4 M −1 s −1 , respectively. It was observed that the immobilized BCA can retain around 91% of its initial activity up to 30 days at 4 °C, which showed higher storage stability than free BCA. Reusability studies suggested that immobilized BCA could keep high activity after 20 cycles of carbonation reaction. BCA immobilized on GFS (BCA-GFS) were used for hydration of CO 2 . The CO 2 sequestration capacity in terms of conversion CO 2 to calcium carbonate was quantified by organic elemental analysis. The amount of CaCO 3 precipitated over GFS-BCA was nearly the same as that precipitated over free BCA, 227 mg of CaCO 3 /mg of BCA-GFS as compared to CO 2 sequestration capacity of 241 mg of CaCO 3 /mg of BCA.
研究领域:
生物化学
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环境科学
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材料科学
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产品关联
DOI
高影响因子
PNAS Nexus
·
2023
·Vol. 2
·期 3
·
IF 0
Cui Li,Xin Yuhan,Yang Kai,Li Hongzhe,Tan Fengjiao,Zhang Yulong,Li Xingrui,Zhu Zhi,Yang Jun,Kao Shuh-Ji,Ren Bin,Zhu Yong-Guan,Musat Florin,Musat Niculina
Microbial interactions impact the functioning of both natural and engineered systems, yet our ability to directly monitor these highly dynamic and spatially resolved interactions in living cells is very limited. Here, we developed a synergistic approach coupling single-cell Raman microspectroscopy with 15N2 and 13CO2 stable isotope probing in a microfluidic culture system (RMCS-SIP) for live tracking of the occurrence, rate, and physiological shift of metabolic interactions in active microbial assemblages. Quantitative and robust Raman biomarkers specific for N2 and CO2 fixation in both model and bloom-forming diazotrophic cyanobacteria were established and cross-validated. By designing a prototype microfluidic chip allowing simultaneous microbial cultivation and single-cell Raman acquisition, we achieved temporal tracking of both intercellular (between heterocyst and vegetative cells of cyanobacteria) and interspecies N and C metabolite exchange (from diazotroph to heterotroph). Moreover, single-cell N and C fixation and bidirectional transfer rate in living cells were quantified via SIP-induced characteristic Raman shifts. Remarkably, RMCS captured physiological responses of metabolically active cells to nutrient stimuli through comprehensive metabolic profiling, providing multimodal information on the evolution of microbial interactions and functions under fluctuating conditions. This noninvasive RMCS-SIP is an advantageous approach for live-cell imaging and represents an important advancement in the single-cell microbiology field. This platform can be extended for real-time tracking of a wide range of microbial interactions with single-cell resolution and advances the understanding and manipulation of microbial interactions for societal benefit.
研究领域:
微生物学
·
分子生物学
·
环境科学
✓ 已验证
DOI
PubMed
Journal of Environmental Chemical Engineering
·
2025
·
IF 7.2
Xiuduan Yang,Boyuan Tan,Dehui Kong,Jiarun Duan,Xueying Zhao,Gan Wang,Xiaojun Niu,Lijuan Zhang,Li Yang,Bekchanov Davronbek,Xintai Su
s The green synthesis of artificial humic acid (AHA) is a key approach to the efficient utilization of biomass waste resources. To reduce dependence on strong alkaline agents (KOH, NaOH, etc.) in current humification methods, this study proposes using Saline-alkali soil (SAS) as a cost-effective substitute for producing AHA from biomass solid waste. The results of the study indicated that an AHA yield of 23.33 % ± 1.12 % was achieved through pyrolytic humification under optimal conditions (250 °C, 2 h, with SAS to cotton stalk (CS) ratio of 4: 1). Characterization analysis demonstrated that the SAS supplied an alkaline environment throughout the humification process. Furthermore, the mineral elements in the SAS (Na + , K + , Fe 3+ , and Al 3+ ) facilitated the decomposition of lignin to generate polyphenolic structures, which promoted the protein condensation and increased the aromaticity of AHA. Carbon flow analysis revealed that 60.5 % of the carbon was immobilized, with AHA contributing up to 25.6 % of the total immobilized carbon. Hydroponic experiments demonstrated that the addition of AHA at a concentration of 200 mg L −1 significantly enhanced wheat growth. The residual carbon soil cultivation experiment revealed that the residual carbon exhibited a high specific surface area (6.57 m 2 g −1 ) and effective adsorption properties, achieving a 16 % immobilization rate of Pb and alleviating heavy metal stress on plants. This study presents an innovative approach to the integrated management of SAS and converting biomass solid waste into 'carbon-negative emissions'.
研究领域:
环境科学
·
土壤学
·
农业化学
·
生物资源技术
·
可持续农业
✓ 已验证
产品关联
DOI
高影响因子
FUEL
·
2025
·
IF 7.5
Dawei Wu,Aijia Zhang,Jing Liu,Yingju Yang
MFe 2 O 4 (M = Ca, Mn, Co, Ni, Cu and Zn) spinel sorbents were synthesized by a low-temperature sol–gel auto-combustion method for arsenic adsorption from flue gas. CuFe 2 O 4 spinel exhibited the highest arsenic removal performance among these sorbents, and the adsorption capacity reached 14.09 mg/g at 800 °C. The pseudo-first order, pseudo-second-order and Elovich kinetic models can well predict arsenic removal process, which indicates that the mass transfer and chemisorption processes are important during arsenic capture by CuFe 2 O 4 . The impact of incineration flue gas components on As 2 O 3 adsorption was elucidated. It was found that HCl showed negligible effect on arsenic removal by CuFe 2 O 4 sorbent while CO 2 , H 2 O, SO 2 and NO played inhibition roles. X-ray photoelectron spectroscopy results revealed that the adsorbed arsenic exists in the forms of As 3+ and As 5+ on the sorbent surface. The chemisorption and oxidation mechanisms are responsible for arsenic adsorption by CuFe 2 O 4 . The toxicity characteristic leaching procedure and sequential extraction experiments demonstrated that arsenic can be considerably immobilized in the CuFe 2 O 4 sorbent. The high arsenic capture capacity, excellent HCl resistance and low risk of secondary contamination make CuFe 2 O 4 an efficient sorbent for gaseous arsenic capture.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS
·
2019
·
IF 2.752
Chongyan Hao,Yuan Liao,Yang Wu,Yajing An,Jiannan Lin,Zhengfei Gu,Minhong Jiang,Shi Hu,Xiaotian Wang
In this work, we report RuO 2 -loaded TiO 2 –MXene hybrid nanostructure as a promising photocatalyst possessing remarkable activity for photocatalytic nitrogen fixation in water at room temperature. The MXene was synthesized by HF etching of the precursor Ti 3 AlC 2 and used as the substrate for the growth of RuO 2 nanoparticles and TiO 2 through a hydrothermal reaction . The benign activity is mainly attributed to the synergetic effect among MXene, TiO 2 , and RuO 2 in photocatalytic ammonia synthesis by combining light harvesting and nitrogen activation.
研究领域:
材料科学
·
环境科学
·
化学工程
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Xiao-Dong Cheng,Zheng Zhang,Yun-Ping Li
Green chromatography techniques using low-toxic mobile phase are getting increasingly attention in recent years. The core is developing stationary phases that possess adequate retention and separation under the mobile phase of high content water. Using thiol-ene click chemistry, an undecylenic acid-bonded silica stationary phase (UAS) was prepared in a facile manner. Elemental analysis (EA), solid-state 13 C NMR spectroscopy and Fourier transform infrared spectrometry (FT-IR) confirmed the successful preparation of UAS . The synthesized UAS was employed for per aqueous liquid chromatography (PALC), which uses little organic solvent during separation. Due to the hydrophilic carboxy, thioether group and hydrophobic alkyl chains of the UAS, various categories of compounds (including nucleobases , nucleosides , organic acids and basic compounds) with different properties can achieve enhanced separation under the mobile phase of high content water compared with commercial C18 and silica stationary phases. Overall, our present UAS stationary phase shows excellent separation ability toward highly polar compounds and meets the requirements of green chromatography.
研究领域:
分析化学
·
色谱技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2014
·
IF 4.258
Qianying Sheng,Xindai Su,Xiuling Li,Yanxiong Ke,Xinmiao Liang
A-saccharide-based stationary phase for hydrophilic interaction liquid chromatography (HILIC) is presented. The method uses carbonyl di-imidazole (CDI) as a cross-linker for dextran in aqueous solution. Different from the traditional immobilized saccharide stationary phase, this method was using a simple way to bond the high molecular of polysaccharide immobilized on the silica gel. The new method avoided the time-consuming process but had a very considerable loading result. With silica-based surface bonded, it will be possible to develop well-defined surface modifications that promote the hydrophilic with dextran and high mechanical strength with silica. For all tested compounds, including polar compounds and carbohydrates, this dextran-bonded stationary phase performed well in terms of separation efficiency and column stability, and the retention mechanism matched that of typical HILIC retention. Moreover, good selectivity was achieved in the separation of oligosaccharides and glycopeptides.
研究领域:
分析化学
·
色谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Energy Materials
·
2020
·
IF 4.473
Huijuan Han,Yang Yang,Jiafang Liu,Xiuzhen Zheng,Xulin Wang,Sugang Meng,Sujuan Zhang,Xianliang Fu,Shifu Chen
Photocatalytic nitrogen fixation is considered as a very promising technology to solve the high-energy consumption problem in industrial ammonia synthesis. Because of the mildness of reaction conditions, its development is still limited by the low reaction efficiency and unknown reaction mechanism. Inspired by the mechanism of biological nitrogen fixation in nature, Zn3In2S6 with different sulfur sources is prepared to study the effects of cation defects on the adsorption, activation, and reaction in photocatalytic nitrogen fixation. Because of the electron-rich property of zinc vacancies in Zn3In2S6 nanosheets, it can effectively activate the N≡N triple bond, thereby increasing the rate of kinetic reduction. With the activation of N2 molecules on zinc vacancies, the existing protons obtained from methanol (solvent) will greatly accelerate the electron transfer between interfaces during the nitrogen reduction reaction. Notably, Zn3In2S6 with rich Zn vacancies exhibits higher activity (355.2 mg L–1 gcat–1, 15 times) than Zn3In2S6 with poor vacancies in nitrogen fixation. This work describes the contribution of zinc vacancies to the fixation and activation of nitrogen molecules, which is very important to establish a highly effective system of photocatalytic nitrogen fixation.
研究领域:
材料科学
·
催化化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）