Metabolites
·
2025
·Vol. 15
·期 11
·709
·
IF 3.7
Yongmei Chen,Qingwen Wei,Jing Wang,Chuanyao Huang,Zhenghao Wang,Yuchun Liu
Background:Pleioblastus amarus(Keng) Keng f. is widely distributed in southern China and serves as both a medicinal and edible resource. Flavonoids represent the main bioactive compounds in this species, contributing to its antioxidant, anti-inflammatory, and anti-tumor activities.Methods:In this study, a targeted metabolomics approach based on liquid chromatography–tandem mass spectrometry (LC–MS/MS) was applied to analyze 35 flavonoid compounds in dry leaf and shoot samples ofP. amarus. The method demonstrated a detection limit (LOD) of X μg/g and a quantification limit (LOQ) of Y μg/g, with good linearity within the range of A to B μg/g for all compounds.Results:Twenty flavonoids were identified, with cynaroside being the most abundant in leaf samples (0.013 mg/g) and isovitexin being the most abundant in shoot samples (0.001 mg/g). The total flavonoid content in dry leaf (0.19 mg/g) was significantly higher than in dry shoot (0.022 mg/g). Multivariate statistical analyses revealed clear clustering and metabolic differences between the two tissues, and fifteen differential metabolites were identified by orthogonal partial least squares discriminant analysis (OPLS-DA). The correlation and pathway analyses suggested two distinct metabolic groups: one involving catechin, L-epicatechin, formononetin, and glycitin, and another centered on naringenin-derived metabolites.Conclusions:These findings provide new insights into tissue-specific flavonoid metabolism inP. amarus, highlighting distinct patterns in the leaves and shoots. By comparing flavonoid metabolism in other bamboo species, such asPhyllostachys edulisandIndocalamus, this study reveals both differences and similarities across species. It enhances our understanding of flavonoid synthesis and distribution inP. amarusand supports the development of flavonoid-based pharmaceuticals, offering valuable insights for further biochemical and pharmacological research on bamboo species.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Applied Food Research
·
2026
·Vol. 6
·期 1
·101723
·
IF 6.2
Dahe Qiao,Chun Yang,Xiaozeng Mi,Sihui Liang,Yan Guo,Juan Chen,Zhengwu Chen
Tea quality is synergistically shaped by raw material grade and processing, yet existing research has primarily focused on the processing stage, with systematic evaluations of the role of raw materials remaining insufficient. This study systematically investigated the flavor chemistry of the 'Qiancha 1′ (QC1) tea plant cultivar across tea types from multi-grade raw materials. Fresh leaves of QC1 (one-bud-one-leaf, one-bud-two-leaves, one-bud-three/four-leaves, open surface leaves) were processed into eight distinct tea products (green, black, oolong). Key taste components (catechins, theanine, caffeine, 20 amino acids, 18 flavonoids) were quantified via HPLC and LC-MS. Aroma volatiles (366 compounds) were analyzed using headspace solid-phase microextraction coupled with comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry (HS-SPME/GC × GC-TOF-MS). Multivariate analysis revealed significant compositional shifts driven by raw material grade and processing. Tea from one-bud-two-leaves exhibited the highest theanine content. The curling process markedly increased amino acids and aroma volatiles while reducing catechins and flavonoids levels. Lipids and esters dominated the volatile profile. Thirty-eight key aroma-active compounds were identified, defining QC1′s characteristic floral, green, and fruity notes. This work provides a chemical basis for optimizing QC1 tea processing, recommending one-bud-one/two-leaf raw materials for curly green or black tea to enhance characteristic flavor quality.
✓ 已验证
产品关联
DOI
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2023
·
IF 6.5
Ruiyi Fan,Congyi Zhu,Diyang Qiu,Genlin Mao,Bernd Mueller-Roeber,Jiwu Zeng
As a well-recognized traditional Chinese medicine (TCM), immature fruits of Citrus grandis ‘Tomentosa’ (CGT) serve to cure chronic cough in humans. Specialized metabolites including flavonoids may have contribute to this curing effect. Knowledge about the molecular mechanisms underlying flavonoid biosynthesis in ‘Tomentosa’ fruits will, therefore, support the breeding of varieties with improved medicinal properties . Hence, we profiled the transcriptomes and metabolites of the fruits of two contrasting C. grandis varieties, namely ‘Zheng-Mao’ (‘ZM’) used in TCM production, and a locally cultivated pomelo, namely ‘Guang-Qing’ (‘GQ’), at four developmental stages. A total of 39 flavonoids, including 14 flavanone/flavone, 5 isoflavonoids , 12 flavonols, and 6 anthocyanins , were identified, and 16 of which were quantitatively determined in the fruits of the two varieties. We found that ‘ZM’ fruits contain more flavonoids than ‘GQ’. Specifically, rhoifolin levels were significantly higher in ‘ZM’ than in ‘GQ’. We annotated 31,510 genes, including 1,387 previously unknown ones, via transcriptome sequencing of ‘ZM’ and ‘GQ.’ A total of 646 genes were found to be differentially expressed between ‘ZM’ and ‘GQ’ throughout at all four fruit developmental stages, indicating that they are robust expression markers for future breeding programs. Weighted gene co-expression network analysis identified 18 modules. Combined transcriptional and metabolic analysis revealed 25 genes related to flavonoid biosynthesis and 16 transcriptional regulators (MYBs, bHLHs, WD40) that may be involved in the flavonoids biosynthesis in C. grandis ‘Tomentosa’ fruits.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2024
·
IF 2.6
Chen Xian,Zhang Menghan,Zheng Lang,Deng Xiaoqin,Chen Qiang,Han Wendi,Huang Zhengjun,Weng Shaohuang
The establishment of a convenient and efficient testing method is crucial and needed for the monitoring of diosmin. In this study, nitrogen doped carbon dots (N-CDs) with the particle size distribution of 2.5–5.7 nm and the average diameter of 4.1 nm were successfully synthesized using a simple strategy. N-CDs exhibited excellent and stable fluorescence performance with the quantum yield of 22.33%. Correspondingly, a fluorescence analytical method was developed for diosmin determination using N-CDs as probe. UV-vis absorbance spectroscopy and the evaluation of internal filtering parameters verified that the mechanism causing the quenching of N-CDs was an internal filtering effect (IFE). The concentration of diosmin can be directly evaluated based on the quenched fluorescence intensities. After optimizing experimental conditions, it was found that the fluorescence quenching efficiency ((F 0 -F)/F 0 ) of N-CDs exhibited a good linear relationship with the concentration of diosmin (C Diosmin ) in the range of 3.0–50 µg/mL. The limit of detection (LOD) was 0.86 µg/mL based on 3σ/slope ( n = 13). This method was successfully applied to accurately determine the content of diosmin in diosmin tablets and human plasma samples with good reproducibility. It stands out for its simplicity, speed, and acceptable determination performance.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Future Foods
·
2025
·
IF 7.2
Mengyao Wu,Xiaole Chen,Lei Zhang,Yingbin Shen,Tak-Ho Lo,Xinquan Yang
Termitomyces species are edible mushrooms that have been documented to possess beneficial medicinal properties. Termitomyces intermedius is a recently identified species that has not yet been fully investigated for its potential effects on obesity. This study aimed to evaluate the anti-obesity effects of Termitomyces intermedius methanolic extracts (TIE) in C57BL/6J mice subjected to a high-fat diet (HFD). Over a 12-week period, the mice were treated with TIE at daily doses of 75 and 150 mg/kg. A comprehensive analysis of obesity-related indicators, serum metabolomics, gut microbiota composition, and flavonoid profiling was conducted. Our results indicated that high dosage of TIE intervention significantly prevented weight gain by nearly 60% when compared to control group, along with improved glucose homeostasis, lowered circulating triglycerides, cholesterols, low-density lipoprotein-cholesterols, alanine aminotransferase, aspartate aminotransferase levels and increased high-density lipoprotein cholesterols. TIE supplementation reduced hypertrophy of hepatocytes and epididymal adipocytes. Metabolomic analysis identified 2,788 metabolites in total and TIE enriched 30 of them, including 7 metabolic pathways associated with lipid and amino acid metabolism. Furthermore, the intervention was associated with an enrichment of beneficial bacterial taxa including Muribaculaceae, Blautia and a reduction in potentially harmful microbes including Alistipes and Bacteroides , suggesting a positive modulation of the gut microbiome. Flavonoid profiling revealed the rich abundance of apigenin, luteolin and quercetin which are believed to contribute to its anti-obesity properties. As the first study to investigate the pharmaceutical properties of Termitomyces intermedius , this work underscores the multifaceted anti-obesity pathways of TIE, including the synergistic impact of its flavonoid constituents. These findings provide a robust theoretical basis for further exploration of TIE's therapeutic potential in personalized nutrition and dietary interventions for obesity management.
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2025
·
IF 6.6
Jiayi Zhang,Jingzhi Wang,Junfeng Wang,Weifeng Ni,Shichao Liu,Daqing Zhao,Zhengqi Dong,Shiting Yu,Siming Wang,Weinan Wang,Meichen Liu
Sun-Dried Ginseng (DG), Red Ginseng (RG), and Black Ginseng (BG) have significant nutritional and medicinal values, with remarkable differences in color and flavor. Current research on the sensory characteristics of these products has predominantly focused on the Maillard reaction, whereas pigment accumulation and broader non-enzymatic reaction pathways remain underexplored. This study explored how carotenoid/flavonoid dynamics and non-enzymatic pathways contribute to the sensory properties of processed ginseng products. The accumulation of pigments such as violaxanthin-myristate-caprate and dihydromyricetin was observed, which may contribute to the orange-red color and sweet aftertaste of RG and BG. Additionally, 47 metabolites related to amino acids, carbohydrates, and fatty acids [variable importance in projection (VIP) > 1.2, p < 0.05] and 8 key flavor substances with relative odor activity value (ROAV) > 1 were identified. Correlation analysis confirmed strong associations between these compounds and color and flavor attributes, highlighting the roles of carotenoids and flavonoids in sensory formation. Meanwhile, the Light Gradient Boosting Machine (LightGBM) algorithm was used to predict the relative contributions of carotenoids and flavonoids to the sensory characteristics of different processed ginseng products.
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）