MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yan Song,Weiyang Gao,Liang Sun,Junda Bai,Yi Zhang,Jingnan Zhu,Hua Xing,Chongyang Liang
The large decline in breast cancer mortality has been driven by rapid development and continuous progress in diagnostic techniques. However, it remains difficult to diagnose malignant lesions in distal branching ducts using mainstream techniques such as immunofluorescence ultrasound, molybdenum target, and ductoscopy. Combined with existing reports, we found that CEA level by ELISA in 13 patient 's nipple aspirate fluids (NAF) may be positively correlated with the malignancy of breast lesions. Here, considering the low content of CEA in NAF and the need for automated techniques for batch clinical testing, a magnetic induced mixing surface-enhanced Raman spectroscopy technique (SERS) was used to quantitatively analysis CEA concentration in NAF. In the appearance of CEA, an antibody-conjujated silver nano-probe (AgNPs-Ab1/4-ABP) can be linked onto the surface of another antibody-conjujated magnetic-probe. This immune sandwich-like structure enables more AgNPs-Ab1/4-ABP to remain in situ in the magnetic field. The magnetic spontaneous collection effect brings second SERS signal amplification at 1140 cm −1 approximately. After testing 10 clinical samples, the signal of Magnetic-Field Amplified SERS was not affected by the complex components in NAF, and a good linear range of 0.5–20 ng mL −1 could be achieved. This method might potentially be useful in the differential diagnoses of benign lesions with breast cancer and clinical follow-up of non-surgical intervention nipple discharge patients.
研究领域:
医学
·
生物化学
·
肿瘤学
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DOI
高影响因子
CHROMATOGRAPHIA
·
2016
·Vol. 79
·期 5
·327-333
·
IF 1.332
Peng Xiaofeng,Wang Huawen,Yang Binbin,Zhan Xin,Wu Yiwei
To improve the sensitivity of capillary electrophoresis, field-amplified sample injection (FASI) was developed to determine bisphenol A (BPA), α -naphthol ( α -NAP) and β -naphthol ( β -NAP) in drinks and lake water. Parameters (sample matrix, concentration of NaCl, water plug length, sample injection time and voltage) affecting FASI have been systematically investigated. Under optimum conditions, the sensitivity was improved 17.1-, 15.8- and 9.9-fold for BPA, α -NAP and β -NAP, respectively. The detection limits of BPA, α -NAP and β -NAP were 0.071, 0.038 and 0.081 μg mL −1 and the proposed method has been successfully applied to detect BPA, α -NAP and β -NAP in drinks and lake water with recoveries of 82.0–109.3 %.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
·
2011
·
IF 2.733
Haiyan Wang,Yuchao Lu,Jie Chen,Junchao Li,Shuhui Liu
The extraction and determination of cytisine , sophocarpine, matrine , sophoridine and oxymatrine in Sophora flavescens Ait. were performed using subcritical water extraction and capillary electrophoresis with field-amplified sample stacking. The effects of extraction temperature, pressure, time and cycle number on the extraction yields were investigated systematically for accelerated solvent extraction with ethanol (ASE) and accelerated solvent extraction with water (subcritical water extraction, SWE). The extraction yields obtained using SWE, ASE, water ultrasonic extraction and chloroform soaking extraction methods were compared. The electrophoresis separation buffer was monosodium phosphate (pH 3.0; 110 mM)-isopropanol (85:15, v/v). The effect of phosphoric acid added to the sample matrix on the reproducibility of the peak heights of the analytes was also examined. Cytisine, sophoridine and oxymatrine showed good linearity ( R 2 > 0.999) within 0.125–4.0 μg/mL, and sophocarpine and matrine exhibited good linearity ( R 2 > 0.998) within 0.0625–2.0 μg/mL, with the detection limits in the range of 0.004–0.0013 μg/mL. The five alkaloid contents in medicinal plants from different sources and Sophora instant granule were determined and compared.
研究领域:
药物分析
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天然药物化学
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分离科学
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药用植物学
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DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2016
·Vol. 40
·期 3
·789-797
·
IF 2.741
Liangfei Ouyang,Qian Liu,Heng Liang
A common drawback of paper-based separation devices is their poor detection limit. In this study, we combined field-amplified sample stacking with moving reaction boundary electrophoresis on a paper chip with six array channels for the parallel separation and concentration of multiple samples. With a new hyphenated technique, the brown I 2 from the Fe 3+ /I − oxidation–reduction reaction emerged near the boundary between the dilute ethylene diamine tetraacetic acid and potassium iodide and highly concentrated KCl solutions. For the separation and concentration of three components, Cr 3+ , Cu 2+ , and Fe 3+ , the Fe 3+ detection limit was improved at least 266-fold by comparing the hyphenated technique with moving reaction boundary electrophoresis. The detection limit of Fe 3+ was found to be as low as 0.34 ng (20 μM) on the paper chip. We also demonstrated the analysis of a real sample of four metal ions, with detection limits as follows: 0.16 μg Cr 3+ , 1.5 μg Ni 2+ , 0.64 μg Cu 2+ , and 1.5 μg Co 2+ . The synergy of field-amplified sample stacking and moving reaction boundary electrophoresis in the micron paper-based array channels dramatically improved the detection limit and throughput of paper-based electrophoresis.
研究领域:
分析化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
Food Analytical Methods
·
2017
·Vol. 11
·期 4
·1155-1162
·
IF 2.038
Zheng Yang,Peng Xiaofeng,Wu Yiwei
A sensitive method has been established by using activated carbon as an adsorbent for extraction of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) from bean sprout, tomato, potato, cucumber, and wheat grain prior to field-amplified sample injection-micellar electrokinetic capillary chromatography (FASI-MEKC) detection. Parameters affecting FASI and solid-phase extraction (SPE) were optimized in detail. Under the optimal conditions, the sensitivity of SPE-FASI-MEKC for IAA and IBA was respectively improved by 32.5 and 29.7 times as compared with the direct MEKC. The detection limits were 4.1 for IAA and 5.3 ng mL −1 for IBA with 4.9–5.2% RSD ( n = 9, C = 0.03 μg mL −1 ). The new method has been successfully applied to detect IAA and IBA in bean sprout, tomato, potato, cucumber, and wheat grain with the recoveries of 81.7–110%.
研究领域:
植物学
·
分析化学
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DOI
高影响因子
JOURNAL OF ANALYTICAL CHEMISTRY
·
2014
·Vol. 70
·期 1
·81-86
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IF 0.812
Cui Shuya,Liu Jiaqin,Hu Xiaoli,Li Juan
A novel, simple and sensitive method for the enantioseparation of terbutaline enantiomers was developed using capillary zone electrophoresis ( CZE ) in combination with partial filling technique and field-amplified sample concentration. Under optimal conditions, the racemic terbutaline was resolved in less than 21 min by partially filling a fused-silica capillary with 15 mM β-cyclodextrin ( β-CD , 1.9 psi, 90 s) and carrying out an electrophoresis with 50 mM phosphate buffer (pH 1.8) at 15 kV separation voltage. The sensitivity was further improved by using field-amplified sample injection (0.2 psi, 2.5 s water plug). Compared with conventional CZE, the resolution and sensitivity of two enantiomers were much improved (the detection limit decreased by 18–25-fold).
研究领域:
分析化学
·
药学
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DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 7
·4011-4016
·
IF 3.108
Zhi-Yong Wu,Biao Ma,Song-Fang Xie,Kun Liu,Fang Fang
In this report, we introduced an electrokinetic strategy for simultaneous concentration and separation of charged analytes in an open and free standing paper fluidic channel. This was achieved by taking advantage of the field amplified sample stacking (FASS) effect. An electric field gradient was developed in the channel due to the conductivity difference between the background electrolyte (BGE) and sample solution. Through adjustment of the electroosmotic flow level and BGE concentration, charged analytes were not only stacked but also separated in the gradient based on their difference in electrophoretic mobility. Proof of concept was demonstrated firstly by two food dyes, brilliant blue and amaranth, and each dye was about 100-fold concentrated within 300 s. Simultaneous stacking and separation of two colored proteins, bovine hemoglobin and cytochrome c, was also demonstrated visually with the proposed method. Sample consumption was reduced to 5 μL by directly loading the sample onto the open channel, and the separation was completed within 30 s. This method is simple, rapid and easy to operate, which is particularly beneficial to the development of sensitive and multifunctional paper-based analytical devices (PADs) for field testing.
研究领域:
分析化学
·
生物化学
·
电泳技术
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产品关联
DOI
高影响因子
Analytical Methods
·
2017
·Vol. 9
·期 18
·2703-2709
·
IF 1.9
Biao Ma,Song-Fang Xie,Lei Liu,Fang Fang,Zhi-Yong Wu
A crucial challenge in development of robust paper-based analytical devices (PADs) is the lack of sensitivity. Electrokinetic stacking is a kind of unique sample preconcentration for low-abundant charged species, which can be integrated to PADs to enhance the sensitivity. In this work, we bring new insights into the mechanism underlying an electrokinetic stacking technique based on a field amplified stacking (FAS) effect implemented on a simple paper fluidic channel. Here, we reveal that the acid–base titration reaction between the cations/anions in the background electrolyte (BGE) and the OH−/H+ ions from water electrolysis plays an essential role in the development of the electric field gradient. This was experimentally confirmed by changing the BGE type, monitoring the current variation and pH characterization. Based on the new knowledge, the paper-based FAS was successfully extended to stack cationic analytes. Eventually, a model protein of bovine hemoglobin (BHb) in its anion form was 150-fold stacked within 60 s. This work shows that the sensitivity of PADs can be greatly enhanced with effective use of this simple and low cost electrokinetic stacking technique.
研究领域:
分析化学
·
生物医学工程
·
电泳技术
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产品关联
DOI
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2020
·Vol. 43
·期 11
·2193-2200
·
IF 2.878
Lei Xu,Weiqi Meng,Jing Lu,Fengjuan Cui,Lidi Gao,Lin Chen,Yang Xin
A sensitive approach of capillary electrophoresis coupled with field-amplified sample injection and transient isotachophoresis was developed for the simultaneous determination of two β-blockers: sotalol and metoprolol. In this dual focusing technique, the samples were prepared via only dissolution in ultrapure water and then injected electrokinetically. Phosphate acted as both the background electrolyte and the leading electrolyte. Its optimized concentration was 80 mM. A total of 25 mM of glycine was used as the terminating electrolyte. Under optimum conditions, good separation of sotalol and metoprolol was achieved within 10 min. In comparison with the conventional method, the sensitivity enhancement factors were up to 1031 and 919 for sotalol and metoprolol, respectively. The proposed method was employed in the determination of sotalol and metoprolol in spiked human urine samples. The limits of detection and limits of quantitation obtained via ultraviolet detection were 5 and 12 ng/mL, respectively, for sotalol, and 10 and 25 ng/mL, respectively, for metoprolol. The intraday repeatability values were lower than 2.7 and 1.7% for peak area and migration time, respectively. The assay is a simple and efficient strategy with potential for application in clinical and biochemical laboratories for monitoring sotalol and metoprolol.
研究领域:
分析化学
·
临床医学
·
药理学
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DOI
高影响因子
LAB ON A CHIP
·
2016
·Vol. 16
·期 18
·3460-3465
·
IF 5.586
Biao Ma,Yi-Zhen Song,Ji-Cheng Niu,Zhi-Yong Wu
We present a novel electrokinetic stacking (ES) method based on field amplification on a simple paper device for sample preconcentration. With voltage application, charged probe ions in a solution of lower conductivity stack and form a narrow band at the boundary between the sample and the background electrolyte of higher conductivity. The stacking band appears quickly and stabilizes in a few minutes. With this ES method, three orders of magnitude signal improvement was successfully achieved for both a fluorescein probe and a double-stranded DNA within 300 s. This enhanced stacking efficiency is attributed to a focusing effect due to the balance between electromigration and counter electroosmotic flow. We also applied this ES method to other low-cost fiber substrates such as cloth and thread. Such a simple and highly efficient ES method will find wide applications in the development of sensitive paper-based analytical devices (PADs), especially for low-cost point-of-care testing (POCT).
研究领域:
分析化学
·
即时诊断
·
生物化学
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DOI
PubMed
高影响因子
Food Analytical Methods
·
2014
·Vol. 7
·期 10
·2153-2162
·
IF 1.802
Cheng Heyong,Han Chao,Xu Zigang,Liu Jinhua,Wang Yuanchao
In this study, we reported bromine speciation analysis using a microchip electrophoresis-inductively coupled plasma-mass spectrometry (MCE-ICP-MS)-hyphenated system with sensitivity enhancement by the field-amplified sample injection technique. Satisfactory separation of bromide and bromate within 35 s was achieved in the 1.5-cm separation channel under an electric field of approximately −260 V cm −1 in the buffer environment of 65 mM NaAc-HAc (pH 8.0). The sensitivities were improved by 12.8 for bromide and 12.0 for bromate by preparing the standards or samples in 3 mM sodium acetate instead of the background electrolyte. The detection limits for Br − and BrO 3
− were 0.18 and 0.22 μg L −1 , respectively, and the precisions of the migration time and reproducibilities of peak height and area were all lower than 4 %. Under the optimized conditions, the proposed method was successfully applied for speciation analysis of bromine in bread samples. Recoveries of BrO 3
− and Br − in six bread samples were between 97.2 and 105.2 %. The good agreement between the determined values by the proposed MCE-ICP-MS method and the high-performance liquid chromatography (HPLC)-ICP-MS method was obtained. All results prove its advantages including high sensitivity, high efficiency, and low operation cost, which are beneficial to routine analysis of bromine speciation in environmental, biological, and food fields.
研究领域:
分析化学
·
环境科学
·
食品科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）