Xuehua Ruan,Kaimin Zhang,Xiaobin Jiang,Xiujuan Zhang,Xiaoming Yan,Ning Zhang,Gaohong He
Homoporous membranes are promising for size-sieving filtration, but their prospect is restricted by the lack in mechanical strength . A facile and low-cost route based on in situ breath figure and thermal adhesion method is proposed to fabricate the reinforced homoporous MF membranes in this research. The substrates specific to this route are fabricated by PVDF coating and then ice sealing on nonwoven fabrics . On the tailor-made substrates, the porous film is formed as usual by the breath figure method, and then immobilized on the substrate conveniently by PVDF melting. Acetone is introduced into the casting solution with intent to accelerate solution spreading on ice surface and facilitate micron-scale water droplets floating down to the solution film, so that the resistance layer could be formed thinner and abundant in perforated pores to attain high water permeation flux. Four usually used homoporous membrane materials, i.e., PSF , PES, PPO and PEI , have succeeded in membrane fabrication. FE-SEM images reveal that membrane pores are perforated and homogeneous with controllable size, again the homoporous layer is tightly adhered on substrate. Water permeation, particle sieving and regeneration tests indicate that the reinforced homoporous membranes made by this novel method are practicable for high-resolution separation under moderate pressure and long-time service.
Peng Xie,Xiaoyue Yao,Zhenyu Chu,Yang Yang,Haifeng Li,Siyuan Tan,Haodong Tang,Jiahua Zhou,Wanqin Jin
The detection of circulating tumor cells (CTCs) in peripheral blood is a novel and accurate technique for the early diagnosis of cancers. However, this method is challenging because of the need for high collection efficiency due to the ultralow content and similar size of CTCs compared with other blood cells. To address the aforementioned issue, we proposed a homoporous polydimethylsiloxane (PDMS) membrane and its microfilter device to perform the ultrafast isolation and identification of CTCs directly from peripheral blood without any labeling treatment. The membrane pores can be homogenously controlled at a size of 6.3 μm through the cross-linking time of PDMS during a filtration-coating strategy. Within only 10 s, the designed device achieved a retention rate greater than 70% for pancreatic cancer cells, and it exhibited excellent cell compatibility to support cell proliferation. The isolated CTCs on this membrane can be easily observed and identified using a fluorescence microscope.
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