Biology-Basel
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2020
·Vol. 9
·期 7
·171
·
IF 3.796
Jianming Zhang,Yushan Bu,Chengcheng Zhang,Huaxi Yi,Daqun Liu,Jingkai Jiao
At present, De Man, Rogosa and Sharpe (MRS) broth is the medium of choice for promoting bacteriocin production. However, this medium is expensive and not applicable for large-scale production. Therefore, a low-cost and high-efficiency culture medium for bacteriocin Lac-B23 production byLactobacillus plantarumJ23 was developed. First, the effects of the composition of MRS broth on bacteriocin Lac-B23 production and bacterial growth were researched by a one variable at a time approach. Then, a Plackett-Burman design was used to screen significant components for production. Finally, the steepest ascent and central composite designs were used to obtain an optimum medium. The final composition of the modified MRS was much simpler than MRS broth, and the modified MRS contained only glucose, yeast extract, dipotassium phosphate, manganese sulfate monohydrate, Tween 80 and sodium acetate anhydrous. The highest bacteriocin Lac-B23 production reached 2560 activity units (AU)/mL in the modified MRS, which is nine times higher than that in MRS broth (280 AU/mL). Meanwhile, the cost per liter of the modified MRS (8.56 Ren Min Bi (RMB)/L) is 34.70% the cost of MRS broth (13.11 RMB/L), and the cost per arbitrary units of bacteriocin Lac-B23 in the modified MRS is approximately fourteen times more convenient (3.34 RMB/106AU) than in the MRS broth (46.82 RMB/106AU).Keywords:bacteriocin Lac-B23;low-cost;high-efficiency;culture medium
研究领域:
微生物学
·
食品科学
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DOI
PubMed
高影响因子
BIOMEDICAL CHROMATOGRAPHY
·
2015
·Vol. 30
·期 4
·601-605
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IF 1.723
Zhaoli Ling,Ping Xu,Zeyu Zhong,Fan Wang,Nan Shu,Ji Zhang,Xiange Tang,Li Liu,Xiaodong Liu
A new pre-column derivative high-performance liquid chromatography (HPLC) method for determination of d -glucose with 3- O -methyl- d -glucose (3-OMG) as the internal standard was developed and validated in order to study the gluconeogenesis in HepG2 cells. Samples were derivatized with 1-phenyl-3-methy-5-pyrazolone at 70°C for 50 min. Glucose and 3-OMG were extracted by liquid–liquid extraction and separated on a YMC-Triart C 18 column, with a gradient mobile phase composed of acetonitrile and 20 m m ammonium acetate solution containing 0.09% tri-ethylamine at a flow rate of 1.0 mL/min. The eluate were detected using a UV detector at 250 nm. The assay was linear over the range 0.39–25 μ m ( R 2 = 0.9997, n = 5) and the lower limit of quantitation was 0.39 μ m (0.070 mg/mL). Intra- and inter-day precision and accuracy were <15% and within ±3%, respectively. After validation, the HPLC method was applied to investigate the gluconeogenesis in Dulbecco's modified Eagle medium (DMEM) cultured HepG2 cells. Glucose concentration was determined to be about 1–2.5 μ m in this gluconeogenesis assay. In conclusion, this method has been shown to determine small amounts of glucose in DMEM successfully, with lower limit of quantitation and better sensitivity when compared with common commercial glucose assay kits. Copyright © 2015 John Wiley & Sons, Ltd.
研究领域:
生物化学
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医学检测
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药理学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
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2020
·Vol. 73
·期 3
·552-562
·
IF 1.636
Liang Wang,Enyan Gao,Man Hu,Qiang He,Yuting He,Xiaoyan Zheng
Milk beer is a new type of dairy beverage in China and increasingly popular with consumers. The strain FXJ1 isolated in our laboratory was an excellent strain for the production of milk beer with alcohol content of 0.48%. Our research verified that the strain FXJ1 was superior to the commercial strain MJ for milk beer production and got the optimal medium formula (67.37 g/L sucrose, 29.7 g/L yeast extract, 15.61 g/L corn steep liquor, 4.13 g/L KH 2 PO 4 and 0.3 g/L MgSO 4 ) which allowed the biomass production of strain FXJ1 to achieve 8.88 ± 0.08 g/L and increase 2.94-fold as compared with yeast extract peptone dextrose base medium.
研究领域:
食品科学
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微生物学
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DOI
高影响因子
ELECTROPHORESIS
·
2019
·Vol. 40
·期 20
·2665-2671
·
IF 2.754
Wei Wang,Ruiguang Bai,Huimin Zhang,Xiaoyu Cai
Corynebacterium glutamicum ( C. glutamicum ) is a well-known workhorse for the industrial production of amino acids. Different carbon, nitrogen, and sulfur source may force the bacterium to produce specific metabolites. In this work, a method of high-speed MEKC with LIF detection was developed to rapidly analyze the amino acid metabolites released by C. glutamicum , which is fed with different culture mediums. Corynebacterium glutamicum was cultured in microbial fuel cells to monitor its metabolism process and collect its metabolites. In the CE system, a microliter-scale sample reservoir was designed and applied to perform tiny volume sample injection. With the assistance of microwave, the derivatization time for amino acids with FITC was greatly shortened to 6 min. Under the optimized condition, the eight candidate amino acids of metabolites could be rapidly separated within 2 min. The whole analysis process for real samples, from sampling to determination, could be shortened to less than 10 min. The results showed that C. glutamicum could produce additional l -lysine and l -valine as the metabolites when fed with glucose and l -methionine, respectively. The method proved that culture mediums used to feed C. glutamicum had great effect on the bacterium's metabolites.
研究领域:
微生物学
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生物化学
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分析化学
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE
·
2025
·
IF 3.9
Song Wang,Liu Song,Yaru Li,Zhenxin Zhao,Ting Xue,Guoxiang Luan,Huarong Guo
Holothurian cell culture remains unsuccessful due to the lacking of suitable medium and tissues. Here, a reliable in vitro cell culture system has been developed for Apostichopus japonicus by optimizing the basal and growth media as well as the sourced tissues. Blood vessel and longitudinal muscle tissues were first found to be the best two sourced tissues. Subsequently, the composition and content of free amino acids and carbohydrates in holothurian coelomic fluid were analyzed and the obtained data were used to design and obtain an optimal basal medium (SCBM-4). Next, an optimal growth medium (SCGM-24) was further developed by supplementing 1 % FBS, 25 % coelomic fluid and 20 % body wall enzymatic hydrolysate of sea cucumbers into SCBM-4. Primary blood vessel (SCBV) cells could be efficiently isolated by mechanical dissociation in tissue dissociation apparatus and survive healthily for 22 d in SCBM-4, and 58 d in SCGM-24 with a gradually decreased proliferation activity. Primary longitudinal muscle (SCLM) cells could only be efficiently isolated by explant culture and healthily survive for 52 d in SCBM-4 but with no proliferation activity, whereas their viabilities decreased sharply in SCGM-24. The epithelial-shaped SCBV cells could be successfully sub-cultured for 3 times using 4 °C PE solution, whereas the loosely attached round-shaped SCBV and SCLM cells could be passaged for 6 and 4 times by pipetting, respectively. Additionally, shrimp covert mortality nodavirus (CMNV) could cross-species infect and multiply in SCBV cells, and acute heat shock treatment could significantly upregulate the transcriptional expression of heat shock protein genes.
研究领域:
海洋生物学
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细胞培养
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病毒学
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分子生物学
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水产养殖
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产品关联
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2025
·
IF 9
Ming Yang,Ning Xiang,Yunan Tang,Yue Li,Ximing Zhang
Ammonia accumulation poses a major challenge in high-density cell production, including cultivated meat. This study presents sodium zirconium phosphate (NaZrP) as an efficient ammonia adsorbent, synthesized via a minimal solvent thermal method. In discarded culture medium, NaZrP reduced ammonium concentration by 56 % and maintained efficiency over four reuse cycles. At 0.5–2.5 g/L, it sustained over 90 % cell survival after 24 h, confirming biocompatibility. The addition of disodium hydrogen phosphate improved NaZrP’s properties by increasing layer spacing, ion adsorption rate, and adsorption capacity. Adsorption followed pseudo-second-order kinetics and the Langmuir model, indicating monolayer adsorption. Chemisorption was the dominant mechanism, with ion exchange occurring under weakly alkaline conditions. NaZrP exhibited minimal glucose adsorption and negligible impact on solution pH. Its rapid and selective ammonia removal, combined with reusability and cell compatibility, makes it a promising solution for managing ammonia toxicity in large-scale cell cultures. Further optimization will enhance its potential for cost-effective applications in cultivated meat and other biotechnological industries.
研究领域:
生物技术
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细胞工程
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食品科学
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DOI
高影响因子
Microorganisms
·
2024
·Vol. 13
·期 1
·22
·
IF 4.1
Hyun-Jin Kang,Hwan Hee Yu,Chang-Won Cho,Young Kyung Rhee,Tae-Wan Kim,Young-Wook Chin
Yeast formulations such as dry yeast are essential for supplying microbial starters to the alcoholic beverage industry. In Korea, the expensive freeze-drying method is used to manufacture brewer’s dry yeast, and therefore an economical process such as fluidized bed drying is needed. In the dry yeast manufacturing process, the medium and drying conditions are key factors that determine its quality and manufacturing cost. In this study, we aimed to optimize the medium composition and fluidized bed drying conditions for the efficient production of dry yeast. Muscovado and corn steep liquor were used as the carbon and nitrogen sources, respectively, and their optimal concentrations were identified using response surface methodology for efficient cultivation ofSaccharomyces cerevisiaeReY4-7 isolated fromnuruk. Central composite design analysis revealed that the optimal medium composition was 146.12 g/L muscovado and 58.68 g/L corn steep liquor. A dry cell weight of 36 g/L was achieved during 24 h of batch fermentation in a 30-L bioreactor containing this medium. Analysis of protective agents against fluidized bed drying revealed Span 80 as the strongest protective agent forS. cerevisiaeReY4-7. Response surface methodology revealed 50 °C and 41.45 min as the optimal fluidized bed drying conditions, under which the viable cell count reached 10.28 log CFU/g, comparable to that of the commercial dry yeast products. Overall, optimization of the medium and drying conditions significantly improved the final cell concentration in the cultivation process and the viable cell count in the drying process of dry yeast.
研究领域:
生物工程
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食品科学
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微生物学
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DOI
PubMed
高影响因子
BIOTECHNOLOGY PROGRESS
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2025
·
IF 2.5
Shumin Zhang,Huimin Huang,Jingwei Zhang,Yuanyuan Zhao,Wen-song Tan,Haibo Cai
Cell culture medium is an important factor affecting the expansion of NK cells in vitro. As an important component of cell culture medium, lipids participate in various complex physiological activities of cells and significantly affect the expansion of cells. Using NK-92 cells as a model, the lipid metabolism of NK cells in vitro was analyzed, and combined with the kinetic relationship between lipid metabolism and NK cell expansion. Four fatty acids, oleic acid, linoleic acid, palmitoleic acid, and α-linolenic acid, were preliminatively identified as the key lipid combinations. The combination was preliminarily verified on the self-developed serum-free medium. It was found that when the key lipid combination was added according to the concentration in the serum, NK-92 cells expansion reached 188.03 ± 33.34-folds, which was significantly higher than 105.28 ± 13.23-folds in the basic medium. Additionally, NK-92 cells expanded by adding key lipid combinations could maintain cell killing function. Overall, this research provides technical support for the development of NK cell serum-free medium.
研究领域:
免疫学
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细胞生物学
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产品关联
DOI
PubMed
高影响因子
Natural Product Communications
·
2024
·
IF 1.5
Meng S. Cheong,Lutfun Nahar,Satyajit D. Sarker,Hui Cao,Wai S. Cheang,Jianbo Xiao
Piceatannol is a stilbenoid, which has shown bioactivities in various cell culture models. However, its stability in cell culture medium is not clear. Here, UPLC-MS/MS was applied in situ to analyze the degradation products of piceatannol in Dulbecco's Modified Eagle's Medium (DMEM) and cell culture to investigate the compound's stability in DMEM. During the incubation with cell culture medium (at 4 and 37 °C), several piceatannol derivatives, such as an oxidation product (m/z 243.06), a reduction product (m/z 247.09), dimers (m/z 485.12 and 487.14) and trimers (m/z 727.18) were detected, which demonstrated the instability of piceatannol in cell culture conditions. To confirm if the new products during the incubation were generated due to the instability of piceatannol, ascorbic acid was added. The presence of ascorbic acid could significantly slow the degradation rate of piceatannol and the generation of piceatannol derivatives, which proved that the new products were generated by the degradation of piceatannol and indicated that the instability of piceatannol might be related to its antioxidant activity.
研究领域:
生物化学
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药物化学
·
分析化学
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产品关联
DOI
高影响因子
Food Frontiers
·
2025
·Vol. 6
·期 2
·1058-1078
·
IF 6.9
Haolin Zhang,Jingjing Zhang,Hui Cao,Petra Högger,Randolph Arroo,Mohamed A. Farag,Avi Shpigelman,Jianbo Xiao,Chunlin Li
A class of flavonoid aminated derivatives that had been consistently overlooked in literature was shown to be formed as artefacts in cell culture medium. In this study, 34 flavonoids from different subclasses were incubated in Dulbecco's modified Eagle's Medium (DMEM) at 37°C for 2 h to identify the reaction mechanism behind aminated derivatives formation from flavonoids. Baicalein, scutellarein, dihydromyricetin, (−)-gallocatechin (GC), (−)-epigallocatechin (EGC), (−)-GC gallate, and (−)-EGC gallate were found to yield corresponding nitrogenous derivatives in both DMEM and amino acid solution. The nitrogen source of these seven flavonoid -aminated derivatives was revealed to be amino acids in DMEM. The pyrogallol group of the flavonoids was a key structural motif, being first oxidized into quinone and then further reacting with amino acids (Strecker degradation) to yield aminated flavonoids and corresponding aldehydes. A slightly alkaline environment accelerated the amination of flavonoids, possibly via the formation of flavonoid quinone. These results provide the mechanistic evidence for the in vitro generation of flavonoid -aminated derivatives, yet to be tested using in vivo assays.
研究领域:
生物化学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CELLULAR PHYSIOLOGY
·
2019
·Vol. 234
·期 9
·16312-16319
·
IF 4.522
Danyang Yue,Mengxue Zhang,Juan Lu,Jin Zhou,Yuying Bai,Jun Pan
We have previously demonstrated that the rate of fluid shear stress (ΔSS) can manipulate the fate of mesenchymal stem cells (MSCs) to osteogenic or chondrogenic cells. However, whether ΔSS is comparable to other two means of induction medium and substrate stiffness that have been proven to be potent in differentiation control is unknown. In this study, we subjected MSCs to 1–7 days of osteogenic or chondrogenic chemical induction, or 1–4 days of 37 or 86 kPa of substrate stiffness induction, followed by 20 min of Fast ΔSS (0–0′) or Slow ΔSS (0–2′), which is a laminar FSS that linearly increased from 0 to 10 dyn/cm 2 in 0 (Fast) or 2 min (Slow) and maintained at 10 dyn/cm 2 for a total of 20 min. We found that 20 min of ΔSS could compete with 5 days' chemical and 2 days' substrate stiffness inductions. Our study confirmed that ΔSS is a powerful tool to control the differentiation of MSCs, which stressed the possible application in MSCs linage specification.
研究领域:
干细胞研究
·
生物力学
·
组织工程
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2018
·
IF 2.441
Hua-Yin Zhang,Ping-Ping Zhang,Xiao-Xin Tan,Ze-Ze Wang,Kao-Qi Lian,Xiang-Dong Xu,Wei-Jun Kang
Lactic acid represents an important metabolite that reflects mitochondria function and may further serve as energy source for cancer cells. In light of this physiological and pathological significance, we developed a novel and sensitive gas chromatography method to detect lactic acid in cell culture media. Here, ethyl chloroformate was selected as derivative reagent and the derivatization process was further optimized in terms of number of reagents and reaction time as well as extraction reagents. Under optimal conditions, good linearity was achieved in the tested calibration range. The limit of detection (LOD) was determined to be 0.67 μmol/L, the recovery rates were 99.6%–106% and the precision rate RSD was <5.49%. Furthermore, this method has been applied to quantify the secretion of lactic acid in cells exposed to mono‑2‑ethylhexyl phthalate at different doses and in cancer cells over time. Taken in concert, our method proved to be both sensitive and reliable and may be applied for studies on mitochondrial function and cell glycolysis conditions.
研究领域:
生物化学
·
医学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）