Water
·
2022
·Vol. 14
·期 23
·3857
·
IF 3.53
Rong Shen,Yichang Yu,Qinfang Liu,Ruili Wang,Rong Lan,Hui Feng,Ran Yu,Lihua Jin,Ye Li
Pefloxacin (PFLX) is a type of fluoroquinolone (FQ) that has been widely used in aquaculture and animal husbandry. It is also a new kind of pseudo-persistent pollutant, which has some toxic effects. In this study, zebrafish (Danio rerio) embryos were used as exposure models to analyze the toxicity of PFLX. The results showed that PFLX was absorbed on the outer membrane surface at the beginning, and then a few PFLX slowly entered the cytoplasm. The adsorption complied with the Langmuir isotherm adsorption model. The surface morphology of the embryonic membrane was changed. PFLX exposure had no effect on fetal autonomous movement, but it significantly inhibited the embryo hatching rate. After PFLX exposure, the embryo and larval survival rates showed a clear dose–effect relationship. Though PFLX exposure did not cause teratogenic toxicity, it still affected cardiovascular function. The potential toxicity mechanism and developmental toxicity of PFLX to zebrafish were revealed. These results could be important for studies concerning FQ toxicology.Keywords:pefloxacin;zebrafish;embryonic membrane;developmental toxicity
研究领域:
毒理学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
Micromachines
·
2023
·Vol. 14
·期 5
·941
·
IF 3.4
Qiuping Li,Nuan Wen,Wu Zhang,Liansheng Yu,Jinghui Shen,Shuxian Li,Yuguang Lv
g-C3N4and g-C3N4/TCNQ composites with different doping levels were prepared using the copolymerization thermal method with melamine as a precursor. XRD, FT-IR, SEM, TEM, DRS, PL, and I-T characterized them. The composites were successfully prepared in this study. The photocatalytic degradation of pefloxacin (PEF), enrofloxacin (ciprofloxacin), and ciprofloxacin (ciprofloxacin) under visible light (λ > 550 nm) showed that the composite material had the best degradation effect on PEF. When TCNQ doping is 20 mg and catalyst dosage is 50 mg, the catalytic effect is the best, and the degradation rate reaches 91.6%, k = 0.0111 min−1, which is four times that of g-C3N4. Repeated experiments found that the cyclic stability of the g-C3N4/TCNQ composite was good. The XRD images were almost unchanged after five reactions. The radical capture experiments revealed that ·O2−was the main active species in the g-C3N4/TCNQ catalytic system, and h+also played a role in PEF degradation. And the possible mechanism for PEF degradation was speculated.Keywords:TCNQ;graphite phase carbon nitride;pefloxacin;photocatalysis
研究领域:
材料科学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIO-MEDICAL MATERIALS AND ENGINEERING
·
2017
·Vol. 28
·期 4
·339-345
·
IF 0.7
Bai Ningning,Tan Cui,Li Qing,Xi Zhongxian
In this paper, a low-cost and multifunctional hydroxyapatite (HA)/pefloxacin (PFLX) drug eluting layer is coated on magnesium (Mg) alloy through a simple hydrothermal and dip process. The drug PFLX could provide effective prevention for bone infection and inflammation due to its broad-spectrum antibacterial property. And HA would promote the growth of new bone and further improve the biocompatibility of implants. Besides, both PFLX and HA exhibits excellent corrosion protection for Mg alloy substrate. This coating is of great value for improving the application of Mg alloy as biomaterials.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Hui Li,Fujia Dong,Yu Lv,Zhaoyang Ma,Yue Chen,Sichun Chen,Jinhua Xian,Yingjie Feng,Sijia Liu,Jiarui Cui,Xiuwei Yan,Songlei Wang
Non-destructive detection of veterinary drug residues in meat products is critical for ensuring food safety and human health. The feasibility of using visible near-infrared (Vis-NIR) and near-infrared (NIR) hyperspectral imaging (HSI) system combined with data fusion for the prediction of pefloxacin residues in mutton was investigated in this study. A partial least squares regression optimization model was developed by multivariate data processing methods. The results showed that the low-level fusion (LLF) produced better results with R 2 P = 0.907 and RMSEP = 0.462 as compared to individual data blocks. Furthermore, intermediate-level fusion (ILF) based on iterative retention of informative variables algorithm showed the best results with R 2 P = 0.940 and RMSEP = 0.375. Finally, the visualized distributions of Vis-NIR and NIR based on the optimal model combination were mapped. The results have demonstrated that a combination of HSI with data fusion may be applied to rapidly and non-destructively detect pefloxacin residues in mutton.
研究领域:
食品科学
·
光谱学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
Plasmonics
·
2025
·
IF 4.3
Chen Kun,Ma Chaoqun,Chen Guoqing,Yang Taiqun,Gao Hui,Li Lei,Hu Anqi,Cao Jun,Zheng Chenkai,Ma Longyao,Chen Zehao
The detection of pefloxacin (PEF) in milk is of significant importance for food safety. Traditional methods for detecting PEF often rely on techniques such as high-performance liquid chromatography (HPLC), which are complex and time-consuming. This article introduces a surface-enhanced Raman scattering (SERS) substrate based on a composite of covalent organic frameworks (COFs) and silver nanoparticles (AgNPs) for the rapid detection of PEF in milk. After testing PEF in both water and milk, the detection limits were calculated to be 6.31 μg/L for water and 82 μg/L for milk, with correlation coefficients of 0.991 and 0.998, respectively. Furthermore, the average recovery rate of PEF in milk ranged from 96.25% to 106.17%, with a relative standard deviation (RSD) of 4.32% to 8.88%. This work offers a simple and rapid method for detecting PEF in milk. Graphical
研究领域:
食品安全
·
分析化学
·
药物残留检测
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2020
·
IF 3.232
Jie Zhao,Xiao Guo,Qiang He,Qiang Song,Fei Wu,Chenhui Zhang
A novel solvothermal process for synthesizing InNbO 4 nanomaterials was developed. In this manner, a series of InNbO 4 samples was synthesized. It was shown that reaction temperature and precursor pH had strong influence on the attributes of InNbO 4 samples. The X-ray diffraction patterns revealed that all the samples possessed monoclinic structure and the optimal reaction condition was found at 250 °C with a pH of 5. Scanning electron microscopy images of different InNbO 4 samples showed various morphologies. Transmission electron microscopy verified the synthesized InNbO 4 -pH 5 was single-crystal cubes . X-ray photoelectron spectra verified the existence of In, Nb, and O in InNbO 4 -pH 5 sample. The band gap of InNbO 4 -pH 5 was calculated to be 2.51 eV. The photocurrent intensity of InNbO 4 -pH 5 was the highest among the prepared samples. The photocatalytic degradation of pefloxacin was investigated using these samples. The InNbO 4 -pH 5 exhibited best degradation efficiency among these samples. The removal efficiency of pefloxacin with InNbO 4 -pH 5 could reach 80.2% in 60 min. Based on free radical capture results, superoxide radicals and holes showed to be the dominant active species. In addition, UHPLC/MS/MS was used to identify the degradation intermediates. Five new pefloxacin degradation products were found and possible degradation pathways were suggested.
研究领域:
材料科学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Qiang Zhang,Shengbao Duan,Yinong Huang,Jingjing Tian,Jia Hu
By integrating the fluorescence of quantum dots (QDs) and Mn 2+ -pefloxacin mesylate (Mn 2+ -pefloxacin), a new type of dual-band fluorescence biosensor for high-efficiency and sensitive determination of double-stranded DNA (dsDNA) is developed. The biosensor is based on the fluorescence "OFF-ON" mode of both QDs and QDs-Mn 2+ -pefloxacin. The Mn 2+ -pefloxacin complex can quench the QDs fluorescence via photoinduced electron transfer (PET), and its fluorescence is also quenched. Due to the specificity and strong binding affinity of dsDNA for the Mn 2+ -pefloxacin complex, it can break the low fluorescent QDs-Mn 2+ -pefloxacin and restore the fluorescence of QDs and Mn 2+ -pefloxacin complex in their respective bands. Therefore, the dual-band fluorescence quantitative detection of dsDNA by QDs-Mn 2+ -pefloxacin can be achieved, while bovine serum albumin , single-stranded DNA, and bio-related ions do not yield similar results. Furthermore, the possible reaction mechanisms are systematically discussed. The detection limits (3δ/K) of herring sperm (hs) DNA in the fluorescence recovery bands of QDs and Mn 2+ -pefloxacin complex are 0.0142 and 0.0465 μg/mL, respectively. The developed biosensor was used for dsDNA detection in synthetic samples, and desirable results are obtained.
研究领域:
生物化学
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 182
·期 1
·297-306
·
IF 3.719
Liang Wanjun,Liu Zhengqing,Li Dan,Wu Xiaoping,Liu Shaopu,He Youqiu
We describe a novel fluorescent bioprobe for the sensitive and selective detection of double-stranded DNA (dsDNA). It consists of quantum dots (Q-dots) whose fluorescence is quenched (through photoinduced electron transfer) on covering them with the Al(III)-pefloxacin complex. It is found, however, that dsDNA has an affinity for this complex that is so strong that it can break up the binding to the quantum dots, this leading to a complete recovery of the fluorescence of both the Q-dots and the Al(III)-pefloxacin complex. Dual fluorescence (peaking at 420 and 547 nm) is observed under a single excitation wavelength of 350 nm. Neither ribonucleic acid, bovine serum albumin, nor biologically relevant metal ions are capable of giving this effect. The detection limit (3σ/k) for herring sperm DNA is 15.3 μg L −1 if determined via the fluorescence of the Q-dots, and 48 μg L −1 via the Al(III)-pefloxacin complex. The method was applied to the determination of hsDNA in synthetic samples and gave excellent results.
Al 3+ -pefloxacin mesylate (QDs-Al 3+ -PFLX) complex acts as an excellent quencher of the fluorescence of QDs, which was attributed to the photoinduced electron transfer from QDs to Al 3+ -PFLX complex. Due to the strong and specific binding of double-stranded DNA with Al 3+ -PFLX complex, the fluorescence intensity of QDs-Al 3+ -PFLX dyads system is restored
研究领域:
生物化学
·
分子生物学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2022
·Vol. 29
·期 28
·43201-43211
·
IF 5.19
Huang Hongli,Zheng Yongxin,Wei Dongning,Yang Guang,Peng Xin,Fan Lingjia,Luo Lin,Zhou Yaoyu
In this paper, one kind of acid–alkali modified sludge-based biochar (ASBC) was synthesized, characterized, and employed as adsorbent for the removal of pefloxacin. The characterization results showed that the specific surface area (SSA) of ASBC (53.381 m 2 /g) was significantly higher than that of SBC (24.411 m 2 /g). ASBC had a rougher surface, larger particle distribution, lower zero point charge, and richer functional groups (e.g., C-O and O–H) than SBC. The adsorption capacity of ASBC was 1.82 times than that of SBC. After 8 adsorption cycles in reuse experiment, the adsorption capacity of ASBC for pefloxacin still reached 144.08 mg/L, indicating that ASBC has good reusability. Static experiments showed that the optimal pH value was 6.0 in the adsorption of pefloxacin on SBC and ASBC. The result of adsorption kinetics indicated that the pseudo-second-order model could describe well the adsorption process. The Freundlich model was better than the Langmuir model to describe the adsorption of pefloxacin by ASBC, indicating that the adsorption process was mainly multilayer adsorption. Thermodynamic result showed that the adsorption of pefloxacin by ASBC was spontaneous and endothermic. The removal mechanism of pefloxacin by ASBC is mainly the substitution reaction and π-π EDA interaction. In summary, acid–alkali modified biochar is an effective adsorbent for pefloxacin in aqueous solution, and has great application prospects. Graphical abstract
研究领域:
环境科学
·
水处理技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MOLECULAR STRUCTURE
·
2022
·
IF 3.841
Ruizhen Li,Yu Hu,Lijun Du,Jiawen Li,Jigang Yuan,Xingyong Liu
In recent years, advanced oxidation processes (AOP) based on sulfate free radicals (SO 4 − ·) have attracted considerable attention due to the excellent advantages of SO 4 − · compared with hydroxyl radical (·OH). However, SO 4 − · generally produced by activating extra persulphates, which causes high cost and risk of secondary pollution. In this work, BiPO 4 modified carbon paper electrode (BiPO 4 /CP) successfully fabricated via simple method can be used as a photocatalyst to effectively activate sulfate originally existed in wastewater to generated sulfate radicals in-situ without adding any extrinsic chemical oxidants. Pefloxacin (PFX) was degraded effectively on BiPO 4 /CP with the help of applied potential. CP with large surface area and high electrical conductivity as an excellent support for BiPO 4 is beneficial to improve the reutilization, electron transfer and photocatalytic performance of BiPO 4 . Moreover, BiPO 4 with large bandgap has sufficient redox capacity to produce not only ·OH, superoxide radical (·O 2 − ), but also SO 4 − ·, which is crucial for photocatalytic pollutant degradation. Detailed characterizations give strong evidences of the successful fabrication of photocatalyst, thermodynamic feasibility of SO 4 − · in-situ formation, the generation of free radicals, and the possible mechanism. Consequently, under the optimal conditions (pH=5.6, +1.0 V (vs.SCE), [SO 4 2− ]=10 mM, 61 μW cm −2 UV light irradiation, 33 mg BiPO 4 loaded on CP), the degradation efficiency of PFX (10 mg/L) was 87.8% in 120 min, which was 5.1 ad 8.2 times higher than that in photocatalytic and electrocatalytic system and 3.3 times higher than that without SO 4 2− . Meanwhile, BiPO 4 /CP maintains high level of degradation efficiency of PFX after 6 cycles under the optimal conditions.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2020
·
IF 7.489
Huijing Ma,Bing Yu,Qingping Wang,Gary Owens,Zuliang Chen
While Fe-based metal-organic frameworks (MOFs) can be used to remove antibiotics by adsorption, knowledge of how antibiotics are degraded by MOFs is still limited. In this study, one Fe-based MOF, NH 2 -MIL-88B was used to remove pefloxacin from aqueous solution via a combination of adsorption and Fenton-like oxidation. NH 2 -MIL-88B exhibited a high adsorption capacity for pefloxacin (41.37 mg·g −1 ), with >99% removal efficiency within 120 min based on Fenton-like oxidation. To better understand the mechanisms involved in integrated adsorption and Fenton-like oxidation, various advanced characterization techniques were used to monitor the changes in morphology and composition of NH 2- MIL-88B before and after removal of pefloxacin. Scanning electron microscopy/energy dispersive X-ray spectroscopy (SEM/EDS), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) all supported adsorption and Fenton oxidation of pefloxacin. In addition, the pefloxacin degradation products identified by LC-UV and LC-MS provided information on the potential adsorption-Fenton oxidation mechanism. These results suggested that NH 2 -MIL-88B has remarkably potential to be used in an integrated adsorption and Fenton-like process for the removal of antibiotics from aqueous solution.
研究领域:
环境科学
·
化学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Qi Liang,Donglan Zhou,Kai Zhang,María B. Camarada,Jianhua Xiong,Xiaoning Liao
Developing a reliable detection method with high performance for pefloxacin (PFL) is crucial for environmental protection and food safety. However, this remains a challenge. In this study, a new portable electrochemical sensor with a smartphone readout system based on a modified Cu-MOF was designed for PFL detection. The modified Cu-MOF was prepared using a two-step post-synthetic modification strategy. Firstly, thiourea (Th) was doped into the Cu-MOF framework using a solvothermal method, and then the Th-Cu-MOF was further functionalized with MWCNTs using a self-assembly method. X-ray photoelectron spectroscopy combining with chronocoulometry technique and electrochemical impedance measurements revealed that Th not only increased the Cu-MOF’s affinity to PFL but also acted as a bridge, enhancing the interaction between Cu-MOF and MWCNTs. This led to a MOF-based nanocomposite with significantly improved conductivity and specific catalytic properties for PFL. After optimizing detection conditions, the portable sensor showed wide linearity range (9 nM–2.5 μM), excellent selectivity, good stability and a low detection limit (2.6 nM) surpassed that of HPLC in the detection of PEL by utilizing square wave voltammetry technique. In real sample analysis of pond water, shrimp flesh and pork, the portable sensor showed good recoveries (87.92 %–104.4 %), which were comparable to those of HPLC and an electrochemical sensor with a classical three-electrode configuration. This demonstrates that the sensor holds excellent potential for on-site monitoring of PFL in aquaculture.
研究领域:
分析化学
·
食品安全
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）