Nanomaterials
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2023
·Vol. 13
·期 2
·247
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IF 5.3
Xu-rui Wang,Zhe-yi Meng,Xue-fen Wang,Wei-long Cai,Ke Liu,Dong Wang
To develop membrane materials with good performance for water purification that are green and low cost, this work reports an organic–inorganic composite membrane composed of silk nanofibrils (SNFs) and palygorskite (PGS). To improve the stability of the the composite membrane, genipin was used as a crosslinking agent to induce the conformational transition of SNF chains from random coils to β-sheets, reducing the swelling and hydrolysis of the membrane. The separation performance can be adjusted by tailoring the component ratio of the nanomaterial. The results showed that these membranes can effectively remove anionic dyes from water, and they exhibit excellent water permeability. The SNF-based membrane had strong mechanical and separation properties, and the PGS could tune the structure of composite membranes to enhance their permeability, so this green composite membrane has good prospects in water treatment and purification applications.Keywords:silk nanofibrils;palygorskite;genipin;water purification;anionic dyes
研究领域:
材料科学
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环境科学
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生物工程
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产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
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2012
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IF 3.628
Yuanting Xu,Li Li,Hao Wang,Xixun Yu,Zhipeng Gu,Chengcheng Huang,Hong Peng
Biological tissues must be chemically fixed before they can be implanted in humans, due to the immediate degradation and presence of antigenicity of naturally derived tissues. To provide a crosslinking reagent which is cytocompatible and may prepare biocompatible fixed tissues, a novel crosslinking agent, alginate dialdehyde (ADA), was employed to fix biological tissues by our group. The study was to evaluate the cytocompatibility of ADA for biological tissue fixation. Glutaraldehyde and genipin counterparts were used as controls. The result suggested that the cytotoxicity of ADA was significantly lower than that of glutaraldehyde and genipin. Additionally, in the evaluation of cytotoxicity of fixed tissue itself and the residues, as well as the cell adhesion property, ADA-fixed tissue was significantly superior to its glutaraldehyde counterpart and comparable to its genipin counterpart. The results obtained in this study demonstrate that ADA is a cytocompatible crosslinking reagent for biological tissue fixation.
研究领域:
生物医学工程
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组织工程
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DOI
PubMed
高影响因子
JOURNAL OF APPLIED POLYMER SCIENCE
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2012
·Vol. 129
·期 2
·689-698
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IF 1.289
Qingchun Zhang,Yan Zhang,Meidong Lang
Microsphere systems have been used to deliver drugs, proteins, and cells. However, dispersed microspheres can induce harmful effects after they are introduced into the body. To agglomerate these dispersed microspheres, an in situ forming system was developed to fabricate microsphere/hydrogel composites. Polycaprolactone microspheres with a porous surface and hollow core were physically incorporated into a genipin-crosslinked gelatin hydrogel. The incorporation of microspheres reduced the swelling capacities and weakened the unexpected volume expansion, which is not favorable for in vivo implants. Additionally, the reacted genipin ratio increased with increasing gelatin concentration. The degradation of the composite was also determined, and it was proposed that the degradation mechanism of the composite was bulk collapse, whereas that for the pure hydrogel was surface erosion. The obtained microsphere/hydrogel composite might have a great potential application as an injectable system for tissue regeneration. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
研究领域:
生物医学工程
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药物递送系统
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材料科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Yun Shao,Ni Gan,Bingbing Gao,Bingfang He
The production of low-lactose and lactose-free milk remains a critical challenge. Novel enzyme immobilization integrated with the flow reactor that could be applied to hydrolyze milk with cost-effectiveness, facilitation, and safety is highly expected. Here, we present a novel continuous-flow bioreactor loaded with β-galactosidase (β-Gal) covalently immobilized 3D-printed scaffolds for the production of lactose-free milk. 3D-printed polylactic acid (PLA) scaffolds modified by self-assembly of polyethyleneimine and sodium hyaluronate layers are rapidly branched with functional groups, followed by covalent immobilization of β-Gal using genipin as the cross-linker. The use of genipin allows efficient and stable immobilization of the enzyme (92.87 % immobilization efficiency), and the immobilized enzyme retains 90.53 % activity after 6th repeated use. Moreover, the 3D-printed scaffolds integrated into the flow reactor perturbed the fluids in the different reactors through directional channels, which improved the internal mass transfer to achieve high catalytic activity (94.20 % lactose hydrolysis efficiency). The lactose residues of milk reacted in the β-Gal flow reactor for 6 and 12 h were 1.87 g dL -1 and 0.38 g dL -1 , which fully met the requirements of the national food safety standards for low-lactose and lactose-free milk. Due to the high efficiency of enzyme immobilization and the stable catalytic activity of the reactor, it has a broad application prospect for the production of low-lactose and lactose-free milk and other biocatalytic fields.
研究领域:
食品科学
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生物工程
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酶技术
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
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IF 6.953
Qiang Liu,Na Xia,Wenjing Wan,Youcheng Gao,Siming Zhu
Novel eco-friendly and green dimethyldiallylammonium chloride (DMDAAC) grafted chitosan/genipin/cellulose hydrogel beads (CCBG-g-PDMDAAC), were fabricated as selective adsorbents for anionic dyes. The physical and chemical structural changes of the prepared hydrogels were evaluated by FTIR, XRD, SEM and TG-DSC analysis. Results showed CCBG-g-PDMDAAC efficiently and selectively adsorb anionic dyes (Reactive Red 195-RR195 and Methyl orange-MO) from mixture of dye solutions. Endowed chitosan-based hydrogels the advantage of acid insolubility and good adsorption. RR195 and MO adsorption process were described better with pseudo-second-order kinetic model and Langmuir isotherm model with a maximum adsorption capacity of 1333.52 and 190.48 mg/g, respectively, indicating that monolayer chemisorption controlled the sorption process. Moreover, the hydrogels exhibited nice reusability and against S. aureus and E. coli . The hydrogels are promising for the potential application in wastewater treatment and antibacterial simultaneously.
研究领域:
环境科学
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材料科学
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生物学
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产品关联
DOI
PubMed
高影响因子
Materials Science & Engineering C-Materials for Biological Applications
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2016
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IF 3.42
Yan Zhang,Lina Mao,Juan Liu,Tianjun Liu
A kind of self-fluorescent, biocompatible, and low-toxic Genipin crosslinked Globin-PEI nanoparticle (Gb-G-PEI NP) with high enzymolysis-stability and photo-stability was synthesized successfully. The properties of the Gb-G-PEI NP were characterized, including its particle size, surface zeta potential, morphology, paclitaxel (PTX) loading capacity and release. The Gb-G-PEI NPs as imaging probe were investigated by Confocal Laser Scanning Microscope (CLSM) in vitro and by fluorescence imaging system in vivo . Cell imaging results showed that the tumor cell line (HepG-2) had the faster cell uptake rate and metabolism rate than the normal cell line (L-O2), this difference showed its tumor selectivity. MTT assay revealed that the PTX-loaded Gb-G-PEI NPs showed almost the equal potence to tumor cell HepG-2 as the free PTX at the same PTX concentration, while a lower cytotoxicity to normal cell L-O2, suggesting its promising utilization as a drug delivery system. The imaging on mice demonstrated the possibility of the self-fluorescent Gb-G-PEI NPs as probe in vivo . So Gb-G-PEI NPs can be potentially utilized as both tracking marker and tumor cell selective drug delivery system in the biomaterial field.
研究领域:
生物材料学
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药剂学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2024
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IF 5.4
Feng Yang,Yilan Wang,Huatao He,Guanxiong Wang,Menghan Yang,Meiying Hong,Jin Huang,Yaolei Wang
Water-in-water (W/W) Pickering emulsions , exhibit considerable potential in the food and pharmaceutical fields owing to their compartmentalization and high biocompatibility . However, constrained by the non-uniform distribution of shear forces during emulsification or the spatial obstruction in polydimethylsiloxane (PDMS) passive microfluidic platform , the existing methods cannot generate monodisperse W/W Pickering emulsions with high particle coverage rate, thereby limiting their applications. Herein, a novel microfluidic system is designed for the preparation of monodisperse and highly particle-covered W/W Pickering emulsions under mild conditions. pH-responsive Polyethylene glycol (PEG)/phosphate aqueous two-phase system (ATPS) is used for the emulsions' preparation. Notably, a coverage rate of 96 ± 3 % is obtained by adjusting the length of the helical coiled tube, as well as the size and contact angle of genipin cross-linked BSA (BSA-GP) particles. Moreover, these W/W Pickering emulsions, with surfaces almost completely covered, can maintain monodisperse (N coal = 1.18 ± 0.03) for one day. Furthermore, the results of ranitidine hydrochloride (RH) release demonstrated that the drug release rate of W/W Pickering emulsions in the simulated gastric fluid (SGF) was 10 times faster than that in the neutral solution. We believe that the highly particle-covered monodisperse W/W Pickering emulsions possess great potential applications in bioencapsulation for foods and drug delivery.
研究领域:
化学工程
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材料科学
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药剂学
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食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）