Scaling DNA synthesis with a microchip-based massively parallel synthesis system
Current high-throughput DNA synthesis technologies use intricate chip and microfluidic systems to produce large-scale synthetic oligonucleotides but with low concentration and limited compatibility for long DNA assembly. Here we report a massive-in-parallel synthesis system, with an ‘identification–sorting–synthesis–recycling’ iteration mechanism applied to microchips for high-throughput DNA synthesis. This approach increases DNA product concentration by four to six orders of magnitude and simplifies downstream processes for large-scale gene synthesis.
CAS / MDL:
148893-10-1
7087-68-5
研究领域:
分子生物学
·
合成生物学
·
生物技术
·
基因工程
·
高通量筛选
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）