Plants-Basel
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2021
·Vol. 10
·期 6
·1112
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IF 3.935
Yan Yang,Liangfang Dai,Decai Wu,Limin Dong,Yisheng Tu,Jiankun Xie,Xiangdong Luo
Huperzia serratais a traditional herb and endangered Chinese medicinal material, which has attracted much attention due to its production of Huperzine A (HupA). In vitro propagation ofH. serratais considered a new way to relieve the resource pressure ofH. serrata. In this study, three different genotypic wildH. serratawere used for in vitro propagation. Then, the antioxidant activity and the content of HupA in the regeneratedH. serratawere investigated. The results showed the survival rate of the explant was increased to 25.37% when using multiple sterilization processes. The best induction medium forH. serratawas the Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg·L−1Naphthalene acetic acid (NAA) and 0.1 mg·L−12,4-Dichlorophenoxyacetic acid (2,4-D), where the regeneration rate of the explant was to 57.04%. The best proliferation medium was the SH medium with NAA (1.0 mg·L−1), as the biomass of in vitro tissue increased 164.17 ± 0.41 times. High-performance liquid chromatography analysis showed that the in vitro culture of three genotypes could produce HupA and the content of HupA was 53.90–87.17 µg·g−1. The antioxidant experiment showed that the methanol extract of in vitroH. serratahad higher antioxidant activity than that of wildH. serrata. This study provides a reliable in vitroH. serrataculture protocol and laid an important foundation for the antioxidant capacity of the thallus and the content of HupA.Keywords:genotypes;Huperzia serrata;Huperzine A;micropropagation;sterilization;antioxidant
研究领域:
植物学
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药用植物学
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生物技术
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DOI
PubMed
高影响因子
PHYTOPATHOLOGY
·
2014
·Vol. 104
·期 10
·
IF 2.746
Amara R. Dunn,Stephen R. Bruening,Niklaus J. Grünwald,Christine D. Smart
Populations of the vegetable pathogen Phytophthora capsici are often highly diverse, with limited gene flow between fields. To investigate the structure of a newly established, experimental population, an uninfested research field was inoculated with two single-zoospore isolates of P. capsici in September 2008. From 2009 through 2012, ≈50 isolates of P. capsici were collected from the field each year and genotyped using five microsatellite loci. The same two isolates were also crossed in the lab. High levels of diversity were detected in the research field, with 26 to 37 unique multilocus genotypes detected each year. Through 2012, genotypic diversity did not decline and no evidence of genetic drift was observed. However, during the 2011 and 2012 growing seasons, four new alleles not present in either parental isolate were observed in the field. Selfing (but not apomixis) was observed at low frequency among in vitro progeny. In addition, evidence for loss of heterozygosity was observed in half of the in vitro progeny. These results suggest that recombination, mutation, and loss of heterozygosity can affect the genetic structure observed in P. capsici populations.
研究领域:
植物病理学
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遗传学
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DOI
高影响因子
SCIENTIA HORTICULTURAE
·
2025
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IF 4.2
Jianbin Shi,Dongfang Fang,Yong Sui,Tian Xiong,Xueling Chen,Chuanhui Fan,Deshun Zhou,Fang Cai,Xin Mei
Sweet potato ( Ipomoea batatas L.) leaves are rich in polyphenols and consumed as leaf vegetable in world. The content and composition of polyphenols are diversity at different harvesting period. This study investigated the changes of total polyphenols content (TPC), antioxidant capacity, and polyphenol fractions content of sixteen varieties of SPLs during growth stage. The results showed significant diversity in the concentrations of active compounds in different genotypes and growth stages. TPC ranged from 27.45 to 243.82 mg CAE/g DW among the varieties over various growth stages, with G3 and E1 varieties exhibiting relatively high TPC and antioxidant capacity. Twelve polyphenol fractions were identified in SPLs. Correlation analysis demonstrated a significant positive relationship between antioxidant capacity and the content of monosubstituted caffeoylquinic acids (3-caffeoylquinic acid, 5-caffeoylquinic acid, 4-caffeoylquinic acid) and disubstituted caffeoylquinic acids (3,4-di-O-caffeoylquinic acid, 3,5-di-O-caffeoylquinic acid, 4,5-di-O-caffeoylquinic acid). Principal component analysis highlighted the superior performance of the E1 variety in terms of polyphenol content and antioxidant capacity.
研究领域:
植物学
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食品科学
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DOI
高影响因子
Insects
·
2020
·Vol. 11
·期 11
·777
·
IF 2.22
Chengfeng Lei,Jian Yang,Jia Wang,Jia Hu,Xiulian Sun
Simple SummaryThe fall armyworm is a notorious lepidopteran pest that consumes many economically important crops. Its recent invasion into China threatens crops in over 19 provinces. This species is susceptible to its homologous nucleopolyhedrovirus (Spodoptera frugiperdamultiple nucleopolyhedrovirus, or SfMNPV). Selection of indigenous isolates that are adaptable in each geographical region is important for developing a virus-based pesticide. In this study, an SfMNPV field isolate was obtained from a natural population of the fall armyworm in Hubei, China. Two genotypes were cloned from the field isolate, and one genotype, SfHub-A, which had similar activity to the field isolate and produced significantly more progeny viruses, was considered to be a suitable strain for the commercial production of SfMNPV. This information will be valuable for developing a virus-based pesticide against fall armyworm in China.AbstractThe fall armyworm,Spodoptera frugiperda, is a new invading pest in China. The baculovirusSpodoptera frugiperdamultiple nucleopolyhedrovirus (SfMNPV) is a pathogenic agent of the fall armyworm and a potential agent for its control in integrated pest management strategies. In this work, we analyze the molecular and biological characteristics of an SfMNPV isolate collected from maize in China (SfMNPV-Hub). Two genotypes were further isolated from SfMNPV-Hub by an in vivo cloning method. The PstI profile of one genotype (SfHub-A) was similar to genotype A of the SfMNPV Colombian isolate, and the other (SfHub-E) was similar to genotype E of the Colombian isolate. The bioactivity of SfHub-A against second-instarS. frugiperdalarvae was not significantly different from that of SfMNPV-Hub, whereas SfHub-E was 2.7–5.5 fold less potent than SfMNPV-Hub. The speed of kill of SfHub-E was quicker than SfMNPV-Hub, while SfHub-A acted slower than SfMNPV-Hub. Occlusion body (OB) production of SfHub-A in anS. frugiperdacadaver was significantly higher than that of SfMNPV-Hub, while SfHub-E yielded far fewer occlusion bodies (OBs) in the host larvae. These results provide basic information for developing a virus-based pesticide against the invading pestS. frugiperda.Keywords:SfMNPV;field isolate;genotype;molecular characterization;biological activity;OB yield
研究领域:
昆虫学
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病毒学
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农学
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DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Yuting Xu,Lili Li,Yumeng Yang,Chengzhi Huang,Hongyan Zou
Developing a simple, reliable, and sensitive hepatitis C virus (HCV) genetic sensing platform is of great significance for diagnosing diseases and selecting appropriate antiviral treatments. Herein, a tandem nucleic acid amplification strategy for sensitive detection of HCV genotype 1b (HCV-1b) was developed by stringing the catalytic hairpin assembly (CHA) and the triggered DNAzyme amplifier. The hairpin reactants were initiated by the target to produce lots of triggering double-stranded DNA sequences which can efficiently activate the subsequent blocked DNAzyme. Thereby, the continuous cleavage of substrate was realized, resulting in the fluorescence signal amplification. The DNA-based isothermal CHA-DNAzyme (CDz) sensing platform was successfully applied for sensitive detection of HCV-1b with the limit of detection (84 pM) and showed good selectivity. Moreover, the practical detection of target DNA in the complex biologic matrix indicated that the developing strategy had good potential for early HCV infection diagnosis.
研究领域:
分子生物学
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病毒学
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诊断学
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DOI
PubMed
高影响因子
Pharmacogenomics & Personalized Medicine
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2021
·
IF 3.912
Xikun Wu,Weichong Dong,Haoran Li,Xiuling Yang,Yiran Jin,Zhiqing Zhang,Ye Jiang
Purpose To perform therapeutic drug monitoring (TDM) of total and free plasma valproic acid (VPA) concentrations in pediatric patients with epilepsy and to analyze related factors.Patients and Methods Pediatric epileptic patients treated in 2015–2019 in our hospital were assessed. Total and free plasma VPA concentrations were obtained by UPLC and LC-MS/MS, respectively. Regression analysis was performed to examine the associations of free plasma VPA with total plasma VPA and plasma protein binding rate. The impacts of individual situation, CYP2C9 genotype, and drug combination on VPA concentration were examined.Results Of the 251 patients, 81 had lower total concentrations than effective therapeutic levels; 86 and 31 patients had infections and central nervous system dysplasia, respectively. VPA’s daily doses and free drug concentrations were significantly lower in the CYP2C9 *3/*3 genotype group versus the CYP2C9 *1/*3 and CYP2C9 *1/*1 groups (P<0.05). Free and total VPA concentrations were linked by Y = 0.0004 X2 + 0.042 X + 0.3035 (r=0.6981); VPA plasma protein binding rate and free VPA concentration were related by Y = 0.0003 X2 - 0.0127 X + 0.9777 (r=0.8136). Both total and free VPA concentrations were significantly decreased in patients simultaneously administered phenobarbital, meropenem and biapenem (P<0.05), with therapeutic failure after meropenem/biapenem co-administration.Conclusion Free VPA amounts have nonlinear relationships with total VPA amounts and plasma protein binding rate in epileptic children. Additionally, CYP2C9 *3/*3 expression affects VPA metabolism. Since phenobarbital affects VPA metabolism, TDM is recommended. Meanwhile, carbapenem-co-administration with VPA should be prohibited.
研究领域:
医学
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药理学
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遗传学
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DOI
高影响因子
ACS Applied Materials & Interfaces
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2019
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IF 8.456
Lihua Chen,Mingchao Liu,Yan Tang,Chuangfu Chen,Xingxing Wang,Zhiqiang Hu
Gold magnetic particles as a new carrier of disease diagnosis probes have attracted wide attention but encountered a bottleneck. That is, the interfacial properties of gold magnetic particles are susceptible to the influence of nonspecific biological molecules in actual diagnostic samples. Here, a novel nanoparticle made by the covalent attachment of polyethyleneimine and hyperbranched polyether polyol onto the gold shell surface of a magnetic bead demonstrated not only low fouling properties but also excellent stability in a variety of external environments, especially in complex biological systems. Most importantly, in its application as the probe for sensitive and selective fluorescence detection of high-risk human papillomavirus (HPV) genotypes 18, 16 in buffer, even in 100% serum, a good linear correlation with the concentration of HPV18/16 target DNA ranging from 5 nM to 1 μM was shown with the low detection limits. To our knowledge, this is one of the few successful examples of direct application of magnetic beads to the detection of disease markers in whole serum, suggesting that this material has good commercial potential and value.
研究领域:
生物医学工程
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分子诊断学
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材料科学
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DOI
高影响因子
PHYSIOLOGIA PLANTARUM
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2018
·Vol. 165
·期 2
·155-168
·
IF 2.58
Wasim Ibrahim,Cheng-Wei Qiu,Can Zhang,Fangbin Cao,Zhu Shuijin,Feibo Wu
Soil salinity and drought are the two most common and frequently co-occurring abiotic stresses limiting cotton growth and productivity. However, physiological mechanisms of tolerance to such condition remain elusive. Greenhouse pot experiments were performed to study genotypic differences in response to single drought (4% soil moisture; D) and salinity (200 mM NaCl; S) stress and combined stresses (D + S) using two cotton genotypes Zhongmian 23 (salt-tolerant) and Zhongmian 41 (salt-sensitive). Our results showed that drought and salinity stresses, alone or in combination, caused significant reduction in plant growth, chlorophyll content and photosynthesis in the two cotton genotypes, with the largest impact visible under combined stress. Interestingly, Zhongmian 23 was more tolerant than Zhongmian 41 under the three stresses and displayed higher plant dry weight, photosynthesis and antioxidant enzymes activities such as superoxide dismutase (SOD), peroxidase (POD) catalase (CAT) and ascorbate peroxidase (APX) activities compared to control, while those parameters were significantly decreased in salt-stresses Zhongmian 41 compared to control. Moreover, Na + /K + -ATPase activity was more enhanced in Zhongmian 23 than in Zhongmian 41 under salinity stress. However, under single drought stress and D + S stress no significant differences were observed between the two genotypes. No significant differences were detected in Ca 2+ /Mg 2+ -ATPase activity in Zhongmian 41, while in Zhongmian 23 it was increased under salinity stress. Furthermore, Zhongmian 23 accumulated more soluble sugar, glycine-betaine and K + , but less Na + under the three stresses compared with Zhongmian 41. Obvious changes in leaf and root tips cell ultrastructure was observed in the two cotton genotypes. However, Zhongmian 23 was less affected than Zhongmian 41 especially under salinity stress. These results give a novel insight into the mechanisms of single and combined effects of drought and salinity stresses on cotton genotypes.
研究领域:
植物生理学
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农学
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胁迫生物学
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DOI
PubMed
高影响因子
TheScientificWorldJOURNAL
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2014
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IF 1.219
Fujia Chen,Xiaohua Long,Zhaopu Liu,Hongbo Shao,Ling Liu
Plant phenolics can have applications in pharmaceutical and other industries. To identify and quantify the phenolic compounds in Helianthus tuberosus leaves, qualitative analysis was performed by a reversed phase high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) and quantitative analysis by HPLC. Ten chlorogenic acids (CGAs) were identified (3-o-caffeoylquinic acid, two isomers of caffeoylquinic acid, caffeic acid, p-coumaroyl-quinic acid, feruloylquinic acid, 3,4-dicaffeoyquinic acid, 3,5-dicaffeoylquinic acid, 1,5-dicaffeoylquinic acid, and 4,5-dicaffeoylquinic acid) by comparing their retention times, UV-Vis absorption spectra, and MS/MS spectra with standards. In addition, four other phenolic compounds, including caffeoyl glucopyranose, isorhamnetin glucoside, kaempferol glucuronide, and kaempferol-3-o-glucoside, were tentatively identified in Helianthus tuberosus leaves for the first time. The 3-o-caffeoylquinic acid (7.752 mg/g DW), 4,5-dicaffeoylquinic acid (5.633 mg/g DW), and 3,5-dicaffeoylquinic acid (4.900 mg/g DW) were the major phenolic compounds in leaves of Helianthus tuberosus cultivar NanYu in maturity. The variations in phenolic concentrations and proportions in Helianthus tuberosus leaves were influenced by genotype and plant growth stage. Cultivar NanYu had the highest concentration of phenolic compounds, in particular 3-o-caffeoylquinic acid and 4,5-dicaffeoylquinic acid compared with the other genotypes (wild accession and QingYu). Considering various growth stages, the concentration of total phenolics in cultivar NanYu was higher at flowering stage (5.270 mg/g DW) than at budding and tuber swelling stages. Cultivar NanYu of Helianthus tuberosus is a potential source of natural phenolics that may play an important role in the development of pharmaceuticals.
研究领域:
药理学
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农业化学
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植物科学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2025
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IF 6.7
Zhen Li,Xingtong Liu,Linna Li,Yandi Hui,Lili Liu,Fujun Yao,Xiaofeng Kang,Yanli Guo
Sickle cell disease (SCD) is an autosomal recessive genetic disease that is caused by a single nucleotide mutation from adenine to thymine in the β-bead protein gene (HBB gene). Currently, there is no completely effective cure for SCD, and management is primarily to prevent. Therefore, timely detection of the genotype is crucial for subsequent diagnosis. In this study, we developed a rapid and straightforward method utilizing the high spatiotemporal resolution of the α-hemolysin (α-HL) nanopore to detect wild-type (HBBW) and mutant (HBBM) HBB gene. This approach uses the anthracycline drug doxorubicin (DOX) as a probe and leverages the distinct characteristic signals generated from its interactions with HBBW and HBBM. When DOX interacts with HBBW, it forms a double-stranded structure of the DNA-DOX complexes, whereas it induces the formation of hairpin structures when interacting with HBBM. These two structures exhibit significant differences in dwell time and amplitude, enabling clear and efficient identification. By mixing different ratios of HBBW and HBBM, we were able to accurately identify genotypes based on the peak area occupied by the characteristic HBBM signals. This method has been successfully applied for detection of HBBM in simulated artificial amniotic fluid (AAF), demonstrating its practicality and reliability. Our approach enables early diagnosis of SCD at the single-molecule level, providing a new avenue for rapid detection of single-nucleotide mutations in gene sequences using DNA–drug interactions.
研究领域:
生物物理学
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遗传学
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分子诊断学
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纳米技术
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药物基因组学
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产品关联
DOI
PubMed
高影响因子
RSC Advances
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2025
·Vol. 15
·期 32
·26362-26370
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IF 4.6
Han Wu,Jiayao Fang,Jiao Chen,Yaoqi Wang,Bo Zheng
Protein display technology enables high-throughput screening and plays an important role in protein discovery and engineering. Conventional in vivo display methods face challenges such as inefficient gene transformation and complex cell proliferation dynamics, while in vitro display methods are often limited to affinity-based selection and suffer from expression bias due to homogeneous reaction conditions. Here, we present a hydrogel particle-based protein display method enabled by particle-templated emulsification. This approach uses functionalized polyacrylamide hydrogel particles as isolated microreactors, incorporating DNA primers for genotype immobilization and Ni-NTA groups for capturing histidine-tagged protein phenotypes. Displayed proteins are synthesized via cell-free protein expression within isolated droplets, overcoming the limitations of in vivo cell culture and enabling compartmentalized screening, which is challenging in conventional homogeneous in vitro systems. Using particle-templated emulsification, single hydrogel particles can be rapidly encapsulated with individual DNA templates into isolated water-in-oil droplets within 30 seconds, without the need for specialized instrumentation. Up to 109 particles can be emulsified in a standard 50 ml conical tube. Compared to conventional droplet microfluidics, particle-templated emulsification achieves higher single-particle encapsulation and improved one-to-one particle–DNA pairing efficiency, reducing reagent consumption and minimizing DNA library loss caused by improper pairing. Digital PCR and cell-free protein expression are sequentially performed within droplets, with both the amplified DNA and the expressed protein immobilized on the same particle, thereby establishing a stable genotype–phenotype linkage. This method eliminates the need for cell handling, enables compartmentalized functional screening, and provides a fast, scalable, and user-friendly workflow for protein display, offering strong potential in directed evolution and protein engineering.
研究领域:
生物化学
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生物技术
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蛋白质工程
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合成生物学
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分子生物学
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微流控技术
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定向进化
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产品关联
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2021
·
IF 7.963
Min Lu,Song Yu,Jiapan Lian,Qiong Wang,Zhenli He,Ying Feng,Xiaoe Yang
To reduce cadmium (Cd) pollution of food chains, screening and breeding of low-Cd-accumulating genotypes have received increasing attention. However, the mechanisms involving Cd tolerance and accumulation are not fully understood. Here, we investigated the physiological responses and metabolomics profiling on two wheat ( Triticum aestivum L.) genotypes, a low-Cd-accumulating genotype in grains (Aikang58, AK58) and a high-Cd-accumulating genotype in grains (Zhenmai10, ZM10), in hydroponic culture treated without/with Cd for 7 days. The results showed that AK58 was a Cd tolerant genotype with higher capacity of antioxidant systems in root. In addition, the concentrations of Cd bound to root cell walls were higher in AK58 than ZM10, of which pectin and hemicellulose played important roles in Cd binding. Moreover, subcellular distribution manifested that Cd sequestrated in the vacuoles was another tolerance mechanism in AK58. Simultaneously, metabolomics profiling showed that, in AK58, phenylalanine metabolism, alanine, aspartate and glutamate metabolism, isoquinoline alkaloid biosynthesis, arginine and proline metabolism, arginine biosynthesis and glyoxylate and dicarboxylate metabolism are highly related to antioxidant defense system, cell wall biosynthesis and metabolisms of phytochelatins together with other organic ligands, playing crucial roles in Cd tolerance and Cd fixation mechanisms in roots. These novel findings should be useful for molecular assisted screening and breeding of low Cd-accumulating genotypes for wheat crop.
研究领域:
植物生理学
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环境科学
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农学
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分子生物学
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DOI
PubMed
高影响因子
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