JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
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2018
·
IF 1.911
Longfei Li,Weizheng Zhang,Mengjia Huang,Jie Li,Jia Chen,Mi Zhou,Jianguo He
The wound healing for tympanic membrane (TM) perforations is one of the most common clinical treatment in otomicrosurgery. Recently, tissue-engineered composites and grafts, also new designs of biomaterials are applied to the management of TM perforation. In this work, a series of gelatin/genipin nanofibrous films were prepared as a patch for repairing TM perforation. Gelatin, a type of biomaterial with excellent electrospinning performance, has been used for preparing the nanofibers. The genipin, as a crosslinking agent, has been blended into the gelatin nanofibers. The reaction between gelatin and genipin engender suitable tensile strength and water-resistance for TM patch. The survival rate of human umbilical vein endothelial cells and fibroblasts demonstrated that the gelatin/genipin nanofiber scaffolds had good biocompatibility, which indicated the genipin was a kind of effective and nontoxic crosslinking agent for improving the mechanical property and water-resistance of gelatin films. In short, our work provides a novel macromolecular material with good mechanical properties, water-tolerance and excellent biocompatibility which could be used as a potential patch for TM repair.
研究领域:
生物材料学
·
组织工程学
·
耳科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Catalysts
·
2021
·Vol. 11
·期 10
·1158
·
IF 4.146
Zhanyong Wang,Tingting Su,Jingjing Zhao
Genipin was used as a crosslinking agent to prepare magnetic genipin-crosslinked chitosan beads, which were then used as a carrier for immobilizing recombinant cutinase fromFusarium solani(FSC) to obtain immobilized FSC. The optimal temperature for the immobilized FSC was 55 °C, which was 5 °C higher than that of the free enzyme, whereas its optimal pH was increased from 8.0 to 9.0; this indicates that the immobilized FSC had improved pH and thermal stability. After repeated use for 10 cycles, the activity of the immobilized FSC remained at more than 50%; after being stored at 4 °C for 30 days, its activity was still approximately 88%. We also found that the Km of the immobilized FSC was higher than that of the free enzyme. These results indicate that the performance of FSC was improved after immobilization, which is an important basis for the subsequent application of FSC in industrial production.Keywords:immobilization;cutinase;chitosan;genipin
研究领域:
生物技术
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酶学
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材料科学
✓ 已验证
产品关联
DOI
高影响因子
Biomedical Materials
·
2024
·Vol. 19
·期 4
·045042
·
IF 3.9
Ling Wang,Xiaoyue Ding,Jingyu Li,Man Li,Peng Ding,Wei Guo,Qiaoyun Wu,Yanfang Sun,Guohua Jiang,Oseweuba Valentine Okoro,Mahta Mirzaei,Amin Shavandi,Lihong Fan,Lei Nie
Bacterial infection can lead to various complications, such as inflammations on surrounding tissues, which can prolong wound healing and thus represent a significant clinical and public healthcare problem. Herein, a report on the fabrication of a novel genipin/quaternized chitosan (CS) hydrogel for wound dressing is presented. The hydrogel was prepared by mixing quaternized CS and genipin under 35 °C bath. The hydrogels showed porous structure (250–500 μm) and mechanical properties (3000–6000 Pa). In addition, the hydrogels displayed self-healing ability and adhesion performance on different substrates. Genipin crosslinked quaternized CS hydrogels showed antibacterial activities against E. coli and S. aureus. The CCK-8 and fluorescent images confirmed the cytocompatibility of hydrogels by seeding with NIH-3T3 cells. The present study showed that the prepared hydrogel has the potential to be used as wound dressing.
研究领域:
生物材料学
·
医学工程
·
药剂学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2025
·Vol. 36
·期 7
·1-16
·
IF 2.8
Yu Ziqing,Gu Yunqing,Ren Yun,Xie Zhengpu,He Chendong,Mou Chenqi,Wu Zhenxing,Wu Denghao,Mou Jiegang
The driving performance of current biomimetic artificial muscles has not yet met the requirements for engineering applications, limiting their practical prospects. This paper presents a test scheme for the driving characteristics of biomimetic artificial muscles, with chitosan and graphene as the main materials. Key evaluation indicators are proposed, including output force, output displacement, and response speed. The study focuses on analyzing the effects of multi-walled carbon nanotubes (MCNT) and Genipin on the driving characteristics of the artificial muscles. MCNT crosslinked with graphene enhances the conductivity of the electrode layer, while Genipin crosslinked with chitosan improves the structural stability and flexibility of the driving layer. The combined addition of MCNT and Genipin significantly enhances the driving performance of the artificial muscles, especially in terms of output displacement, output force, and response speed. The experiment summarizes the optimal additive ratio, providing important guidance and experimental support for further optimization of the design and performance of biomimetic artificial muscles.
研究领域:
生物材料学
·
仿生工程学
✓ 已验证
产品关联
DOI
高影响因子
npj Science of Food
·
2025
·Vol. 9
·期 1
·1-12
·
IF 7.8
Chen Lijia,Zhang Wenjie,Bian Qiqi,Wang Xichang,Zhong Jian
The application of fish oil is limited by its unpleasant fishy flavour, sensitivity to environments (e.g., oxygen, light, and heat), and poor water solubility. The complex coacervation-based encapsulation of fish oil in powders is a promising method to improve the behavior of fish oil in food science and engineering. Controlled preparation of fish oil powders has become an important topic in improving their properties. Herein, the fish oil-encapsulated powders were controlled prepared by combining a fish gelatin (FG)-alginate complex coacervation method with an intra-particulate genipin-crosslinking method. The genipin crosslinking did not affect the particle morphologies, moisture contents, and fish oil encapsulation ability. However, it did affect other physicochemical properties, fish oil oxidation ability, and in vitro digestion behaviors. Especially, the genipin-to-FG mass ratio of 1.0% induced the lowest water activity (0.45 ± 0.01), highest tapped density (0.107 ± 0.007 g/cm 3 ), highest Carr indexes (58% ± 2%), highest Hausner ratios (2.43 ± 0.13), lowest peak value (636.0 ± 10.0 μm) of the powder sizes, and lowest peroxide peak value (442 ± 3 mmol/kg oil). This work provided useful information to understand the relationship between genipin modification and the properties of the fish oil-encapsulated powders.
研究领域:
食品科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Shi-Hao Yang,Xue Zhang,Yu-Mei Tan,Rui Xie,Wei Wang,Zhuang Liu,Da-Wei Pan,Xiao-Jie Ju,Liang-Yin Chu
The controllable fabrication and performances of monodisperse genipin-cross-linked gelatin–chitosan composite (GGC) embolic microspheres with both uniform sizes and adjustable characteristics of elasticity and degradation are reported for the first time. Monodisperse emulsion droplets are fabricated as templates by using gelatin and chitosan aqueous solution as the disperse phase in a microfluidic device, and the chemical cross-linking of gelatin and chitosan inside droplet templates is achieved with genipin that transfers from the collection bath into the droplets. The sizes of droplet templates and the resultant GGC microspheres can be flexibly controlled and predicted by adjusting the fluid flow rates and the device dimensions. The elastic property of GGC microspheres can be flexibly regulated by adjusting the molar ratio of gelatin/chitosan in the disperse phase. The in vitro degradation-caused dynamic volume shrinkage of GGC microspheres can be flexibly regulated by adjusting the molar ratio of gelatin/chitosan, the chitosan deacetylation degree, and the enzyme concentration in degradation solution. Based on the Box–Behnken design-response surface method, a second-order equation is developed for the first time to predict the in vitro degradation-caused dynamic volume shrinkage ratios of microspheres prepared with different molar ratios of gelatin/chitosan and different chitosan deacetylation degrees in degradation solutions with different enzyme concentrations. The fabricated GGC microspheres show good repeatable embolization performances in an in vitro embolization chip system. The results of this study provide valuable guidance for controllable fabrication of degradable microspheres with both uniform sizes and on-demand elastic and degradation characteristics for efficient application in embolization therapy.
研究领域:
生物材料学
·
介入医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CARBOHYDRATE POLYMERS
·
2010
·
IF 3.167
Yanni Zhang,Ying Yang,Tianying Guo
In this work chitosan microspheres have been prepared by ion precipitation using sodium sulfate as precipitant, and crosslinked with natural crosslinker genipin . Then the microspheres were hydrophobically modified by covalent and ion interaction grafting of stearic acid and sodium stearate respectively. Results indicated that the there were still some amount of –NH 2 groups on the genipin-crosslinked chitosan microspheres. And the contact angles for hydrophobical graft microspheres were increased greatly from 27° to 104°, which indicates stearic chains have been grafted on the surface of the microspheres even only by a simple graft process based on ion interaction. The interactions of protein with the hydrophobically modified chitosan microspheres were investigated by surface absorption method using bovine serum albumin as a model protein. And results indicated that adsorption efficiency for bovine serum albumin of the fatty chain grafted chitosan microspheres was higher than that of the prestine one distinctly, due to the additional hydrophobic interaction. And the adsorbed protein could well-off be desorbed under a general condition.
研究领域:
生物材料学
·
蛋白质科学
✓ 已验证
DOI
高影响因子
BIOMACROMOLECULES
·
2015
·
IF 5.75
Xiaojie Song,Hao Wu,Shen Li,Yanfang Wang,Xiaojun Ma,Mingqian Tan
Nanocarriers play an important role in improving the photo- and thermal-stability of photosensitizers to gain better pharmacokinetics behavior in tumor photothermal therapy. Herein, PEGylated chitosan (CG-PEG; PEG: polyethylene glycol) nanoparticles with ultrasmall size (∼5 nm) were prepared through a water-in-oil reverse microemulsion method using genipin as a cross-linker. Particle size and zeta-potential can be tuned by varying the molar ratio between chitosan amino groups and genipin. CG-PEG-ICG (ICG: indocyanine green) nanoparticles were fabricated by adding ICG to CG-PEG aqueous solution through a self-assembly method via electrostatic interaction. The resultant CG-PEG-ICG nanoparticles exhibited improved photo- and thermal-stability, good biocompatibility, and low toxicity. When irradiated with a laser, the cells incubated with CG-PEG-ICG nanoparticles showed very low cell viability (15%), indicating the CG-PEG-ICG nanoparticles possess high in vitro photothermal toxicity. Moreover, the CG-PEG nanocarriers can significantly alter the biodistribution and prolong the retention time of ICG in the mice body after intravenous injection. In vivo photothermal study of tumors injected with CG-PEG-ICG nanoparticles containing ICG at a concentration greater than 100 μg·mL–1 (100 μL) induced irreversible tissue damage. The growth of U87 tumors was dramatically inhibited by CG-PEG-ICG nanoparticles, demonstrating that the CG-PEG nanoparticles may act as potential ICG nanocarriers for effective in vivo tumor photothermal therapy.
研究领域:
生物医学工程
·
纳米医学
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROGRESS IN ORGANIC COATINGS
·
2022
·
IF 6.206
Zhe Xiao,Lin-Hao Liu,Teng Liu,Dan Yang,Xiang Jia,Yu-Kun Du,Shuo-Qi Li,Wen-Jiu Yang,Yong-Ming Xi,Rong-Chang Zeng
Polyelectrolyte-coated magnesium alloy was prepared by layer-by-layer assembly with poly (allylamine hydrochloride) (PAH) and sodium alginate (ALG). The potential influence of genipin as a crosslinking agent on the microscopic features and corrosion resistance of the coatings was examined using Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), potentiodynamic polarization , and electrochemical impedance techniques. An improved anticorrosive property was observed for the crosslinked coating compared to the untreated one, which could be due to the formation of the network barrier against water and corrosion ions such as chloride and oxygen. Furthermore, the cytocompatibility evaluation of different samples by CCK-8 assay suggested that the treatment of genipin was beneficial to the MC3T3-E1 cell proliferation . The study may provide sufficient evidence to show that more efficient LbL assembled multilayer coatings for improving anticorrosion and cytocompatibility would be the ones with crosslinking treatment.
研究领域:
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
·
2024
·Vol. 35
·期 19
·1-13
·
IF 2.8
Ding Hongxin,Gu Yunqing,He Chendong,Ren Yun,Qiu Qianfeng,Hu Chaoxiang,Wu Denghao,Mou Jiegang,Wu ZhenXing
The poor actuation characteristics of current bionic artificial muscles limit their application in engineering practice. Chitosan and graphene are used as the main components to prepare bionic artificial muscles. The driving characteristic indexes of the bionic artificial muscles are proposed, including the output force, output displacement and the respective response speed, and the test platform is built. The mechanism of the influence of Multi-walled carbon nanotubes (MCNT) and Genipin on the actuation characteristics of the bionic artificial muscles is investigated. MCNT crosslinked with graphene enhances the electrical conductivity of the electrode layer, while Genipin crosslinked with chitosan improves the flexibility of the artificial muscles themselves. The bionic artificial muscle with co-addition of Genipin and MCNT showed improvement in output displacement, output force, and response speed. The failure mechanism of the bionic artificial muscle and the improvement mechanism of the additives were analyzed by both electron microscopy and water loss rate, and the changes in the microscopic surface of the electrode layer and the driving layer as well as the dissipation of the internal water were the main reasons for the failure.MCNT and Genipin improved the driving characteristics by enhancing the electrode capacity and accelerating the ion migration rate, respectively.
研究领域:
材料科学
·
生物工程
✓ 已验证
产品关联
DOI
高影响因子
Asian Journal of Pharmaceutical Sciences
·
2018
·
IF 4.56
Yibin Yu,Ruoxi Feng,Jinyu Li,Yuanyuan Wang,Yiming Song,Guoxin Tan,Dandan Liu,Wei Liu,Xinggang Yang,Hao Pan,Sanming Li
The objective of this study was to develop a novel hybrid genipin-crosslinked dual-sensitive hydrogel/nanostructured lipid carrier (NLC) drug delivery platform. An ophthalmic anti-inflammatory drug, baicalin (BN) was chosen as the model drug. BN NLC was prepared using melt-emulsification combined with ultra-sonication technique. Additionally, a dual pH- and thermo-sensitive hydrogel composed of carboxymethyl chitosan (CMCS) and poloxamer 407 (F127) was fabricated by a cross-linking reaction with a nontoxic crosslinker genipin (GP). GP-CMCS/F127 hydrogel was characterized by FTIR, NMR, XRD and SEM. The swelling studies showed GP-CMCS/F127 hydrogel was both pH- and thermo-sensitive. The results of in vitro release suggested BN NLC gel can prolong the release of baicalin comparing with BN eye drops and BN NLC. Ex vivo cornea permeation study was evaluated using Franz diffusion cells. The apparent permeability coefficient ( P app ) of BN NLC gel was much higher (4.46-fold) than that of BN eye drops. Through the determination of corneal hydration levels, BN NLC gel was confirmed that had no significant irritation to cornea. Ex vivo precorneal retention experiments were carried out by a flow-through approach. The results indicated that the NLC-based hydrogel can prolong precorneal residence time. In conclusion, the hybrid NLC-based hydrogel has a promising potential for application in ocular drug delivery.
研究领域:
药剂学
·
生物材料学
·
眼科药物研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Research
·
2024
·
IF 9.5
Chu Huaqing,Zhang Yanyan,Yang Yuan,Xue Jiangtao,Li Cong,Zhang Wei,Li Zhou,Zheng Hui
Acute postoperative pain is commonly treated with flurbiprofen (FBP), but conventional delivery methods are suboptimal. This study prepared a new non-burst release microneedles (MNs) using genipin cross-linked gelatin (cGel). By adding varying amounts of genipin to modulate the crosslinking degree of cGel, the drug release behavior of the drug-loaded MNs in the skin can be altered. The crosslinking parameters that meet therapeutic requirements are selected, thus providing rapid and long-lasting analgesic effects. cGel solutions were successfully cross-linked, altering matrix material microstructure, confirmed by scanning electron microscope imaging and fourier transform infrared spectroscopy. MNs demonstrated increasing mechanical strength with higher crosslinking. Drug release rates were rapid initially, then slowed, exhibiting a characteristic of decreased release rates with increasing degrees of crosslinking. In vivo , FBP/cGel MNs significantly reduced allodynia and hyperalgesia post-surgery, with the greatest effect observed at 2–3 h post-surgery, and can maintain analgesia for up to 6 h. Biosafety tests confirmed good biocompatibility. FBP/cGel MNs effectively penetrate the stratum corneum, safely delivering drugs with significant analgesic effects, excellent mechanical properties, and good biocompatibility, representing a promising strategy for managing acute postoperative pain.
研究领域:
药剂学
·
疼痛管理
·
药物递送系统
·
生物医学工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）