ANALYTICAL CHEMISTRY
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2016
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IF 5.886
Cheng-Yu Li,Di Cao,Ya-Feng Kang,Yi Lin,Ran Cui,Dai-Wen Pang,Hong-Wu Tang
We present an analytical platform by combining near-infrared optical tweezers with two-photon excitation for fluorescence detection of H5N1 virus gene sequences. A heterogeneous enrichment strategy, which involved polystyrene (PS) microsphere and quantum dots (QDs), was adopted. The final hybrid-conjugate microspheres were prepared by a facile one-step hybridization procedure by using PS microspheres capturing target DNA and QDs tagging, respectively. Quantitative detection was achieved by the optical tweezers setup with a low-cost 1064 nm nanosecond pulse laser for both optical trapping and two-photon excitation for the same hybrid-conjugate microsphere. The detection limits for both neuraminidase (NA) gene sequences and hemagglutinin (HA) gene sequences are 16–19 pM with good selectivity for one-base mismatch, which is approximately 1 order of magnitude lower than the most existing fluorescence-based analysis method. Besides, because of the fact that only signal from the trapped particle is detected upon two-photon excitation, this approach showed extremely low background in fluorescence detection and was successfully applied to directly detect target DNA in human whole serum without any separation steps and the corresponding results are very close to that in buffer solution, indicating the strong anti-interference ability of this method. Therefore, it can be expected to be an emerging alternative for straightforward detecting target species in complex samples with a simple procedure and high-throughput.
研究领域:
生物技术
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分子生物学
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光学分析
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DOI
高影响因子
ACS Sensors
·
2024
·
IF 8.2
Yahui He,Jian Zhou,Jinbo Zhang,Yihao Guo,Zhangbin Ji,Hui Chen,Yongqing Fu
Love-mode surface acoustic wave (SAW) sensors show great promise for biodetection applications owing to their low cost, digital output, and wireless passive capability, but their performance is often restricted by the availability of suitable sensitive membrane layers. Herein, a composite layer of electrospun fibers made from cellulose acetate and polyethylenimine, coated with gold nanoparticles, is proposed as a porous and sensitive membrane coated onto a love-mode SAW biosensor for monitoring gene sequences of Staphylococcus aureus. The results showed that the developed sensor exhibited an impressive sensitivity of 122.56 Hz/(nmol/L) for detecting gene sequences of S. aureus, surpassing the sensitivity of conventional SAW sensors employing a bare Au film as the sensitive layer by 5-fold. The analysis revealed a remarkably linear detection (R2 of 0.97827) of S. aureus gene sequences within the range of 0 to 100 nmol/L. The limit of detection was impressively low at 0.9116 nmol/L. The good stability and specificity of the biosensor in liquid environments were demonstrated for clinical diagnostics.
研究领域:
生物医学工程
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
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2011
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IF 5.361
Xiaochun Jiang,Kun Chen,Heyou Han
A novel electrochemical biosensor was developed for detecting short DNA oligonucleotide of Bacillus thuringiensis (Bt) transgenic sequence based on Ag nanoparticle aggregates. To fabricate this DNA biosensor, the thiol-modified capture DNA (cDNA) was first anchored on gold (Au) electrode, and then the target DNA (tDNA) was hybridized with the immobilized cDNA. Subsequently, the probe DNA (pDNA) functionalized by biotinylated Ag nanoparticle was associated with the fixed tDNA, and the single Ag nanoparticle label was obtained (cited as SAg label). Finally, dissociative biotinylated Ag nanoparticle was bound to the resultant biotinylated SAg label assembled on Au electrode by virtue of bridge molecule streptavidin (SA) through biotin–SA specific interaction, which could lead to in situ aggregate of Ag nanoparticles on Au electrode and induce a novel tag including multiple Ag nanoparticles (cited as MAg tag). The novel tag exhibited excellent electroactive property in the solid-state Ag/AgCl process and was successfully applied to Bt transgenic sequence assay. A detection limit of 10 fM was achieved, which was improved by three orders of magnitude as compared to the SAg label. Furthermore, this novel DNA biosensor demonstrated a good selectivity towards tDNA.
研究领域:
生物技术
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分子生物学
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纳米科学
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DOI
高影响因子
CURRENT MICROBIOLOGY
·
2025
·Vol. 83
·期 2
·112
·
IF 2.6
Gao Peng,Lu Xie-Feng,Yao Ling,Xu Yuan-Jia,Zhang Shu-Yi,Liu Guo-Hong,Zhou Shun-Gui
Two novel aerobic, rod-shaped, motile bacterial strains, designated as strains sgz302251 T and sgz5001026 T , were both isolated from paddy soils. 16S rRNA gene sequences analysis revealed that strain sgz302251 T exhibited 98.3% similarity with its closest reference strain Paenibacillus vietnamensis N4 T , while strain sgz5001026 T had the highest similarity of 97.5% to Ectobacillus polymachus KACC 18242 T . The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strains sgz302251 T , sgz5001026 T and their closest reference strains were 86.0% and 21.2%, 84.9% and 22.9%, respectively. The genomic DNA G + C contents of sgz302251 T and sgz5001026 T were 63.4% and 68.8%, respectively. Strain sgz302251 T grew at 20–37 °C (optimum 30 °C), pH 5.0–9.0 (optimum 6.5–7.5) and tolerated 0–1.0% NaCl (optimum 0.5%, w/v), while strain sgz5001026 T grew at 4–45 °C (optimum 37 °C), pH 5.0–9.0 (optimum 6.0) and 0–0.5% NaCl (optimum 0%). The quinone in both strains was MK-7 and major polar lipids were diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and phosphatidylglycerol (PG). The main fatty acids (> 5%) of strain sgz302251 T were anteiso-C 15:0 , anteiso-C 17:0 , and C 16:0 , whereas strain sgz5001026 T included anteiso-C 15:0 , iso-C 15:0 , iso-C 16:0 and iso-C 14:0 . Based on the above evidence, the proposed names of the two novel species are Paenibacillus praedii sp. nov. (type strain sgz302251 T = MCCC 1K09633 T = KCTC 43808 T ), Ectobacillus soli sp. nov. (type strain sgz5001026 T = MCCC 1K09373 T = KCTC 25903 T ), respectively.
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DOI
PubMed
高影响因子
Frontiers in Bioengineering and Biotechnology
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2024
·
IF 4.3
Huang Hao,Li Shuang,Han Xianjing,Zhang Yule,Gao Lingfeng,Wang Xiangjiang,Wang Guiqing,Chen Zhi
Osteoarthritis (OA) has become a serious problem to the human society for years due to its high economic burden, disability, pain, and severe impact on the patient’s lifestyle. The importance of current clinical imaging modalities in the assessment of the onset and progression of OA is well recognized by clinicians, but these modalities can only detect OA in the II stage with significant structural deterioration and clinical symptoms. Blood vessel formation induced by vascular endothelial growth factor (VEGF) occurs in the early stage and throughout the entire course of OA, enables VEGF relating gene sequence to act as a biomarker in the field of early diagnosis and monitoring of the disease. Here in, a facile rapid detection of VEGF relating ssDNA sequence was developed, in which manganese-based zeolitic imidazolate framework nanoparticles (Mn-ZIF-NPs) were synthesized by a simple coprecipitation strategy, followed by the introduction and surficial absorption of probe ssDNAs and the CRISPR/Cas12a system components. Furthermore, fluorescence experiments demonstrated that the biosensor displayed a low detection limit of 2.49 nM, a good linear response to the target ssDNA ranging from 10 nM to 500 nM, and the ability of distinguishing single nucleotide polymorphism. This finding opens a new window for the feasible and rapid detection of ssDNA molecules for the early diagnose of OA.
研究领域:
生物医学工程
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分子生物学
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纳米技术
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诊断学
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DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Xusen Cheng,Bo Liu,Yunfeng Qiu,Ke Liu,Zhuluni Fang,Jinteng Qi,Zhuo Ma,Tiedong Sun,Shaoqin Liu
Commonly used dense arrays of nanomaterials on carbon cloth (CC) are not suitable to accommodate microorganisms in microbial fuel cells (MFCs) due to their unmatched size. To simultaneously enrich exoelectrogens and accelerate the extracellular electron transfer (EET) process, SnS 2 nanosheets were selected as sacrificial templates to prepare binder-free N,S-codoped carbon microflowers (N, [email protected] ) by polymer coating and pyrolysis . N, [email protected] showed a cumulative total charge of 125.70C/m 2 , approximately 2.11 times higher than that of CC, indicating its better electricity storage capacity. Moreover, the interface transfer resistance and diffusion coefficient in bioanodes were 42.68 Ω and 9.27 × 10 -10 cm 2 /s, respectively, superior to CC (141.3 Ω and 1.06 × 10 -11 cm 2 /s). Remarkably, N,S-codoped carbon microflowers excreted more flavin than CC, as confirmed by continuous fluorescence monitoring. Biofilm and 16S rRNA gene sequence analysis revealed that exoelectrogens were enriched, and nanoconduits were generated on the N, [email protected] anode. In particular, flavin excretion was also promoted on our hierarchical electrode, effectively driving the EET process. MFCs equipped with the N, [email protected] anode could deliver a power density of 2.50 W/m 2 , coulombic efficiency of 22.77 %, and chemical oxygen demand (COD) removal amount of 90.72 mg/L/d, higher than that of bare CC. These findings not only demonstrate that our anode is capable of solving the cell enrichment issue, but it may also increase EET rates by bound flavin with outer membrane c-type cytochromes (OMCs) to simultaneously boost the power generation and wastewater treatment performance of MFCs.
研究领域:
环境科学
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材料科学
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微生物学
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电化学
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DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
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2021
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IF 3.101
Xinzhu Chen,Huaying Du,Yanping Hong,Yubo Wang,Mei Shu,Dan Wang,Peng Dai,Weijie Deng,Jianhua Xiong,Wuying Yang
To determine furaltadone metabolites 5-methylmorpholino-3-amino-2-oxazolidinone (AMOZ) residual levels in ctenopharyngodon idellus, direct competitive enzyme-linked immunosorbent assay (dcELISA) was developed based on a single-chain variable fragment (scFv)-alkaline phosphatase (AP) fusion protein. Firstly, VH and VL gene sequences were cloned from hybridoma cell lines secreting monoclonal antibody against AMOZ derivative, and then the productive VH and VL genes were assembled to a scFv gene and engineered to produce scFv-AP fusion protein. The fusion protein was further identified as a bifunctional reagent for immunoassay, then a sensitive one-step dcELISA against AMOZ derivative was developed with a half-maximal inhibitory concentration (IC50) and limit of detection (LOD) of 10.62 and 4.83 ng/mL, respectively. The recovery values of AMOZ from spiked fish samples determined by dcELISA were in 71.31%∼82.88%. The results suggested that the prepared scFv-AP fusion protein in this work can be used for rapid and convenient immunoassays to detect AMOZ residues in fish samples.
研究领域:
免疫学
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生物化学
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分析化学
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水产养殖
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食品安全
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）