APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2021
·Vol. 105
·期 16
·6333-6343
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IF 4.813
Wang Wei,An Chunyan,Yao Yongpeng,Meng Xiangxi,Gao Shu-Shan
The industrially important meta -cresol ( m -cresol, 3-methylphenol) is mainly produced from fossil resources by chemical methods. The microbial production of m -cresol was rarely investigated. Herein, we constructed a platform for the overproduction of m -cresol in a modified fungus Aspergillus nidulans FGSC no. A1145 ∆ST∆EM , which gave a gram-level titer using starch as carbon resource. For the biosynthesis of m -cresol, the 6-methyl salicylic acid synthase (MSAS)-encoding gene patK and 6-methyl salicylic acid decarboxylase-encoding gene patG from A. clavatus were co-expressed in the host A. nidulans . Multiple strategies, including promotor engineering, gene multiplication, and fed-batch fermentation, were applied to raise the production of m -cresol, which resulted in the titers of 1.29 g/L in shaking flasks and 2.03 g/L in fed-batch culture. The chassis cell A. nidulans A1145 ∆ST∆EM was proved to possess better tolerance to m -cresol than yeast, as it could grow in the liquid medium containing up to 2.5 g/L of m -cresol. These results showed that A. nidulans has great potential to be further engineered for industrial production of m -cresol.
Key points
• m-Cresol was de novo biosynthesized by a fungal chassis cell Aspergillus nidulans.
• Promoter engineering and gene multiplication implemented the fine-tuned genes expression.
• The titer of m-cresol reached 2.03 g/L via fed-batch culture.
Graphical abstract
研究领域:
生物技术
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微生物工程
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代谢工程
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DOI
PubMed
高影响因子
Gut Pathogens
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2023
·Vol. 15
·期 1
·1-14
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IF 4.2
Wang Shanshan,Wen Qiuyu,Qin Yan,Xia Quan,Shen Chenlin,Song Shuai
Background The pseudo germ-free (PGF) model has been widely used to research the role of intestinal microbiota in drug metabolism and efficacy, while the modelling methods and the utilization of the PGF model are still not standardized and unified. A comprehensive and systematic research of the PGF model on the composition and function of the intestinal microbiota, changes in host cytochrome P450 (CYP450) enzymes expression and intestinal mucosal permeability in four different modelling cycles of the PGF groups are provided in this paper.Results 16S rRNA gene amplicon sequencing was employed to compare and analyze the alpha and beta diversity, taxonomic composition, taxonomic indicators and predicted function of gut microbiota in the control and PGF groups. Bacterial richness and diversity decreased significantly in the PGF group beginning after the first week of establishment of the PGF model with antibiotic exposure. The PGF group exposed to antibiotics for 4-week-modelling possessed the fewest indicator genera. Moreover, increased intestinal mucosal permeability occurred in the second week of PGF model establishment, indicating that one week of antibiotic exposure is an appropriate time to establish the PGF model. The results of immunoblots revealed that CYP1A2, CYP2C19 and CYP2E1 expression was significantly upregulated in the PGF group compared with the control group, implying that the metabolic clearance of related drugs would change accordingly. The abundance of functional pathways predicted in the gut microbiota changed dramatically between the control and PGF groups.Conclusion sThis study provides information concerning the microbial and CYP450 enzyme expression profiles as a reference for evaluating drug metabolism differences co-affected by gut microbiota and host CYP450 enzymes in the PGF model.
研究领域:
药理学
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微生物学
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分子生物学
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胃肠病学
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DOI
PubMed
高影响因子
Food Bioscience
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2020
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IF 3.067
Zhaoxia Yang,Sasa Liu,Jing Lv,Zeping Sun,Wenhuan Xu,Chaofan Ji,Huipeng Liang,Shengjie Li,Chenxu Yu,Xinping Lin
Chouguiyu (stinky Mandarin fish), a traditional spontaneously fermented fish in China, is famous for its unique aroma and pleasant taste. However, the relationships between the succession of microorganisms during the fermentation and the formation of Chouguiyu 's unique aroma and taste remain to be characterized. In this study, 16S rRNA gene amplicon sequencing was done to investigate the evolving microbiota during Chouguiyu fermentation, and the volatile compounds and amino acids profiles were measured alongside with chemical characterization of the samples (e.g., determination of pH, TVB-N, TBARS , and TCA-soluble peptides). Based on the changes of the microbiota, the fermentation was categorized into two stages: early-fermentation, 1st-3rd days; and late-fermentation, 4th-7th days. Linalool, piperitone, anethole , 1-octen-3-ol, 2-methyl-3-octanone and indole were identified as characteristic volatile compounds in Chouguiyu . According to the sensory evaluation results, the flavor of the Chouguiyu was reinforced in the late-fermentation stage. Redundancy analysis showed the relationships between evolving microbiota and the formation of Chouguiyu 's aroma and taste, and Psychrilyobacter was identified to be the most important bacterial genus contributing to flavor and aroma development, followed by Fusobacterium , Arcobacter and O c eanisphaera . These genera were positively correlated with Glu, Met, Tyr, Pro, linalool, piperitone, indole, TBARS, TVB-N, and TCA , and influenced the flavor and aroma of the Chouguiyu . The results of this study provided a scientific basis to further improve Chouguiyu production.
研究领域:
食品科学
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微生物学
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化学分析
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2020
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IF 5.977
Jin Zhou,Lijuan Yin,Yanan Dong,Lei Peng,Guozhen Liu,Shuli Man,Long Ma
The severity of foodborne diseases caused by foods contaminated by pathogens or their toxins creates an urgent need for the development of specific and sensitive method for detection of bacteria. In this study, taking advantages of CRISPR-Cas13a system, namely, the crRNA programmability and Cas13a “collateral effect” of promiscuous RNase activity upon target RNA recognition, we developed a bacterial sensing strategy with the name of CCB-Detection ( C RISPR- C as13a based B acterial Detection ). Staphylococcus aureus ( S. aureus ) was chosen as a model bacteria for validating the performance of CCB-Detection. Specifically, four steps were carried out: 1) simple extraction of genome DNA ; 2) specific gene amplification by PCR; 3) in vitro transcription; and 4) the “collateral effect” cleavage of reporter RNA to report the analyte signal. It was observed that CCB-Detection was capable to successfully detect the target genomic DNA (gDNA) as low as 10 0 aM. The limit of detection (LOD) was 1 CFU/mL with a dynamic detection range of S. aureus from 10 0 to 10 7 CFU/mL. The entire sample-to-answer time for this biosensor was less than 4 h. CCB-Detection demonstrated satisfactory selectivity for S. aureus without interference from other bacteria. Furthermore, CCB-Detection was successfully applied for sensing S. aureus in real food samples with both known and unknown amounts bacteria (spiked ones and non-spiked ones) and its performance is comparable to the conventional culture-based counting method but with short assay time and high sensitivity. With desirable reliability, sensitivity, specificity and simplicity, herein proposed CCB-Detection could be extended and generalised for other bacterial detection, and has great potential to be used in a wide range of applications such as food safety inspection, disease diagnosis, environment monitoring, etc .
研究领域:
食品科学
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微生物学
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分子生物学
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生物技术
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DOI
PubMed
高影响因子
CHEMOSPHERE
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2023
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IF 8.8
Xuan Zhang,Keke Geng,Ningning Wu,Gang Hu,Ben Fan,Jian He,Wenjing Qiao
4-Chloro-2-methylphenoxyacetic acid (MCPA) is a widely used herbicide across the world. MCPA is persistent and easily transports into anoxic environment, such as groundwater, sediments and deep soils. However, little research on anaerobic microbial degradation of MCPA was carried out. The functional microorganisms as well as the catabolic pathway are still unknown. In this research, an anaerobic MCPA-degrading bacterial consortium was enriched from the river sediment near a pesticide-manufacturing plant. After about 6 months’ acclimation , the MCPA transformation rate of the consortium reached 4.32 μmol g −1 day −1 , 25 times faster than that of the original sludge . 96% of added MCPA (2.5 mM) was degraded within 9 d of incubation. Three metabolites including 4-chloro-2-methylphenol (MCP), 2-methylphenol (2-MP) and phenol were identified during the anaerobic degradation of MCPA. An anaerobic catabolic pathway was firstly proposed: firstly, MCPA was transformed to MCP via the cleavage of the aryl ether, then MCP was reductively dechlorinated to 2-MP which was further demethylated to phenol. The 16S rRNA gene amplicon sequencing revealed a substantial shift in the bacterial community composition after the acclimation. SBR1031, Acidaminococcaceae , Aminicenantales , Syntrophorhabdus , Acidaminobacter , Bacteroidetes_vadinHA17 , Methanosaeta , Bathyarchaeia , KD4-96, Anaeromyxobacter , and Dehalobacter were significantly increased in the enriched consortium after acclimation, and positively correlated with the anaerobic degradation of MCPA as suggested by heat map correlation analysis. This study provides a basis for further elucidation of the anaerobic catabolism of MCPA, and contributes to developing efficient and low-cost anaerobic treatment technologies for MCPA pollution.
研究领域:
环境微生物学
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生物降解与生物转化
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水污染控制
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DOI
PubMed
高影响因子
Frontiers in Microbiology
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2025
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IF 4.5
Weimin Ning,Xuefeng Bao,Lei Jiang,Mei Yang,Tianhao Lei,Maoyan Liu,Yong Liu
Introduction: Tomato bacterial wilt (TBW) is a destructive soil-borne bacterial infection caused by Ralstonia solanacearum. Various nanoparticles have been employed as antibacterial agents to manage TBW via soil application. However, research on the effects of nanoparticles on plant endophytes remains limited.Methods: Here, an analysis of the endophytic bacterial community was performed on healthy and infected tomatoes that were treated with Cu-Ag nanoparticles and thiodiazole-copper via high-throughput 16S rRNA gene amplicon sequencing.Results: The relative abundance levels of beneficial bacteria, including Acidobacteriota, Firmicutes, Actinobacteriota, and Myxococcota, were higher in infected tomato roots treated with Cu-Ag nanoparticles compared with thiodiazole-copper. Functional predictions show that Cu-Ag nanoparticles may affect pyruvate metabolism, oxidative phosphorylation, purine metabolism, carbon metabolism, secondary metabolite production, and the metabolic pathways associated with microbial communities.Discussion: These results could reveal the mechanism by which nanoparticles influence the endophytic microbiomes of plant roots and direct the rational application of nanoparticlesin sustainable agriculture.
研究领域:
植物病理学
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纳米技术
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微生物组研究
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农业科学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Microbiome
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2022
·Vol. 10
·期 1
·1-22
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IF 16.837
Liu Yameng,Zhong Xianchun,Lin Suqin,Xu Hualing,Liang Xinyu,Wang Yibin,Xu Jingyi,Wang Kanglong,Guo Xiaozhen,Wang Jiawen,Yu Minjun,Li Cuina,Xie Cen
ObjectiveHigh intake of caffeoylquinic acid (CQA)-rich dietary supplements, such as green coffee bean extracts, offers health-promoting effects on maintaining metabolic homeostasis. Similar to many active herbal ingredients with high pharmacological activities but low bioavailability, CQA has been reported as a promising thermogenic agent with anti-obesity properties, which contrasts with its poor oral absorption. Intestinal tract is the first site of CQA exposure and gut microbes might react quickly to CQA. Thus, it is of interest to explore the role of gut microbiome and microbial metabolites in the beneficial effects of CQA on obesity-related disorders.Results Oral CQA supplementation effectively enhanced energy expenditure by activating browning of adipose and thus ameliorated obesity-related metabolic dysfunctions in high fat diet-induced obese (DIO) mice. Here, 16S rRNA gene amplicon sequencing revealed that CQA treatment remodeled the gut microbiota to promote its anti-obesity actions, as confirmed by antibiotic treatment and fecal microbiota transplantation. CQA enriched the gut commensal species Limosilactobacillus reuteri (L. reuteri) and stimulated the production of short-chain fatty acids, especially propionate. Mono-colonization of L. reuteri or low-dose CQA treatment did not reduce adiposity in DIO mice, while their combination elicited an enhanced thermogenic response, indicating the synergistic effects of CQA and L. reuteri on obesity. Exogenous propionate supplementation mimicked the anti-obesity effects of CQA alone or when combined with L. reuteri, which was ablated by the monocarboxylate transporter (MCT) inhibitor 7ACC1 or MCT1 disruption in inguinal white adipose tissues to block propionate transport.Conclusion sOur data demonstrate a functional axis among L. reuteri, propionate, and beige fat tissue in the anti-obesity action of CQA through the regulation of thermogenesis. These findings provide mechanistic insights into the therapeutic use of herbal ingredients with poor bioavailability via their interaction with the gut microbiota.
Video Abstract
研究领域:
微生物学
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营养学
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代谢学
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药理学
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肥胖研究
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DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
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2024
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IF 13.3
Yu-Qi Li,Bai-Hang Zhao,Yu-Qing Zhang,Hai-Shan Yang,Bing-Lin Zhang
Multiple nanoparticles presented in wastewater treatment plants can produce comprehensive ecotoxicity. In this study, copper oxide nanoparticles (nCuO) and zinc oxide nanoparticles (nZnO) were investigated for their co-toxicity to aerobic granular sludge (AGS) systems. The combined stress of nCuO and nZnO performed significant antagonistic effects on nitrogen removal both under acute and chronic stress. Under chronic stress, nCuO and nZnO co-existence performed significant antagonistic effects on cell membrane, adenosine triphosphate (ATP) activity and microbial community structure. PICRUSt analysis indicated that nCuO (15 mg/L) co-existence alleviated nZnO (10 mg/L) inhibition on energy production and membrane transport metabolic pathways. In addition, nCuO addition to single nZnO weakened the toxicity of nZnO to nitrogen metabolism, glucose metabolism, and electron transfer genes, contributing to an alleviation in nitrogen removal inhibition. As compared with individual stress, combined stress decreased Cu(II) release from nCuO, but increased Zn(II) release from nZnO. The combination of nCuO and nZnO increased zinc resistance gene amplification and czcC gene proportion, and promoted Zn(II) efferent from cells, also with AGS resistance.
研究领域:
环境工程
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微生物学
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废水处理
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纳米技术
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生态毒理学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）