WORLD JOURNAL OF GASTROENTEROLOGY
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2021
·
IF 5.742
Xiao-Hua Li,Yong-Yi Huang,Lin-Ming Lu,Li-Juan Zhao,Xian-Ke Luo,Ru-Jia Li,Yuan-Yuan Dai,Chun Qin,Yan-Qiang Huang,Hao Chen
BACKGROUND The drug resistance rate of clinical Helicobacter pylori ( H. pylori ) isolates has increased. However, the mechanism of drug resistance remains unclear. In this study, drug-resistant H. pylori strains were isolated from different areas and different populations of Chinese for genomic analysis. AIM To investigate drug-resistant genes in H. pylori and find the genes for the early diagnosis of clarithromycin resistance. METHODS Three drug-resistant H. pylori strains were isolated from patients with gastritis in Bama County, China. Minimal inhibitory concentrations of clarithromycin, metronidazole, and levofloxacin were determined and complete genome sequencing was performed with annotation. Hp1181 and hp1184 genes were found in these strains and then detected by reverse transcription polymerase chain reaction. The relationships between hp1181 or hp1184 and clarithromycin resistance were ascertained with gene mutant and drug-resistant strains. The homology of the strains with hp26695 was assessed through complete genome detection and identification. Differences in genome sequences, gene quantity, and gene characteristics were detected amongst the three strains. Prediction and analysis of the function of drug-resistant genes indicated that the RNA expression of hp1181 and hp1184 increased in the three strains, which was the same in the artificially induced clarithromycin-resistant bacteria. After gene knockout, the drug sensitivity of the strains was assessed. RESULTS The strains showing a high degree of homology with hp26695 , hp1181 , and hp1184 genes were found in these strains; the expression of the genes hp1184 and hp1181 was associated with clarithromycin resistance. CONCLUSION Hp1181 and hp1184 mutations may be the earliest and most persistent response to clarithromycin resistance, and they may be the potential target genes for the diagnosis, prevention, and treatment of clarithromycin resistance.
研究领域:
医学
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微生物学
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基因组学
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DOI
PubMed
高影响因子
PEST MANAGEMENT SCIENCE
·
2025
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IF 3.8
Tian-Yuan Liu,Yu-Xi Zhou,Chen-Yang Yuan,Yi-Fan Gao,Jia-Yao Fan,Guo-Rui Yuan,Jin-Jun Wang,Wei Dou
BACKGROUND Diaphorina citri , a serious pest of citrus, causes significant economic losses due to its role in spreading Huanglongbing. Chemical treatment is the primary method for controlling D. citri . In recent years, D. citri has demonstrated varying resistance levels to a number of pesticides. Combination bioassay and amplicon sequencing approaches can provide a more accurate assessment of resistance in D. citri . RESULTS Field populations of D. citri exhibited significant variability in pesticide susceptibility depending on geographic location. The Guilin population showed the highest imidacloprid sensitivity, with a median lethal concentration (LC 50 ) of 1.14 mg L −1 , indicating a comparatively high susceptibility to this pesticide. In contrast, the Chongzuo population exhibited the lowest sensitivity, with an LC 50 exceeding 2000 mg L −1 , reflecting a significant resistance level. Molecular analysis showed that resistance levels were associated with specific genetic mutations in two key areas: VGSC_L925M, VGSC_M918T, and the acetylcholine receptor α (nAChRα_N88S). Furthermore, there was a correlation between the rate of these mutations and the resistance level. Interestingly, the VGSC_L925M mutation was prevalent in the Chongzuo population, exhibiting a significant frequency of 42.08%. The nAChRα_N88S mutation, found at a low frequency of 0.24%, was unique to the Chongzuo population. CONCLUSION The frequency of VGSC_L925M mutations linked to pyrethroid resistance and nAChRα_N88S mutations associated with neonicotinoid resistance has been revealed in D. citri from major citrus-producing regions in China. We recommend region-specific resistance management strategies, immediate reduction of pyrethroid use in Chongzuo area with high L925M mutation frequencies. This research also provides fundamental data to manage resistance in D. citri . © 2025 Society of Chemical Industry.
研究领域:
昆虫学
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分子生物学
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农业科学
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产品关联
DOI
PubMed
高影响因子
Laser & Photonics Reviews
·
2024
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IF 9.8
Min Fan,Jingbo Chen,Xiaomeng Zheng,Luyun Xu,Jianqin Ye,Xueliang Lin,Kien Voon Kong,Duo Lin,Yudong Lu,Shangyuan Feng
The emergence of “precision medicine” marks a notable shift in cancer treatment, moving from a tumor type–oriented approach to a more targeted, gene-oriented approach. Detecting low-abundance mutant genes in blood is challenging but crucial for personalized treatment plans. Herein, a novel platform combining catalytic hairpin self-assembly (CHA)-mediated self-calibrating surface-enhanced Raman spectroscopy (SERS) with a high-throughput Raman system (CCSPS) was designed. This platform enables ultrasensitive and rapid genotype analysis of gene mutations. The development of CCSPS specifically targets EGFR mutations, which serve as crucial therapeutic targets for precision therapy in lung cancer. This system shows excellent sensitivity and selectivity, capable of detecting multiple EGFR mutations ( Del-19 , L858R , and T790M ) with a detection limit as low as attomolar levels. Additionally, precise genotyping analysis was successfully conducted on 42 clinical samples using the CCSPS, yielding results consistent with those obtained through next-generation sequencing. These results underscore the efficacy of the CCSPS in noninvasively identifying circulating tumor DNA (ctDNA) mutations, facilitating immediate therapeutic decision making at the bedside. In summary, the CCSPS is a fast, accurate, versatile, and compact testing system capable of precisely screening individuals who stand to benefit from targeted therapy, thus promoting personalized and precise healthcare.
研究领域:
医学
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分子生物学
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产品关联
DOI
高影响因子
Materials Today Communications
·
2023
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IF 3.8
Ran Feng,Jiazhi Meng,Hualei Yuan,Xu Zhang,Chunlang Gao,Chaogang Ban,Yizhong Guo,Kaiwen Wang
Cancers, Down’s syndrome, Alzheimer’s and other diseases of gene mutation always occur with anomalous changes of DNA nucleic acid base. Therefore, the development of highly sensitive and reproducible nucleic acid sensors that based upon surface enhanced Raman scattering (SERS) is of immense interest for clinical medical analysis and diagnosis. Herein, a rationally designed core-shell assembly of Au nanoparticle wrapped with Au doped Bi 5 O 7 Br nanotube with eliminated fluorescent background and notable chemical enhancement effect was exploited and served as a SERS sensor for detection of adenine. Especially, we found the fluorescent background was remarkably suppressed in Raman spectra by introducing Au element into Bi 5 O 7 Br. The sensor exhibited a maximum SERS enhancement factor of up to 5.45 × 10 7 and a detection limit as low as 10 −11 M after constructing Au nanoparticle/Au atom doped Bi 5 O 7 Br heterojunction . More importantly, good reproducibility and long term stability under work condition were achieved. To gain in-depth understanding into chemical mechanism (CM) of the Raman signal enhancement, density functional calculations (DFTs) were combined with atom-specific model derived via aberration corrected transmission electron microscope (AC-TEM) to demonstrate the enhanced charge transfer (CT) and substrate-adsorbate adsorption. The doping and coupling strategy holds the potential to fabricate high signal-to-noise ratio SERS sensors for other molecule detection.
研究领域:
生物医学工程
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分子生物学
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分析化学
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产品关联
DOI
高影响因子
ACS Omega
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2018
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IF 0
Wanxing Xu,Shu Xing,Xiaojun Xu,Mengjia Xu,Pan Fu,Tingting Gao,Chao Zhao
With the help of peptide nucleic acid (PNA) and single-stranded (ss) DNA-specific nucleases, a simple, rapid, and label-free nanosensor is demonstrated for the sensitive and accurate detection of single-nucleotide polymorphisms (SNPs) in a homogeneous solution based on the light scattering (LS) of carbon nanotubes. PNAs are non-natural analogues of DNA with extraordinary stability toward enzymatic degradation, and DNAs in the fully matched DNA/PNA duplexes can be effectively protected from ssDNA-specific nucleases, whereas the DNAs in DNA/PNA duplexes involving a mismatch are cleaved into small fragments. This difference could be easily monitored by measuring the LS signal of single-walled carbon nanotubes (SWNTs) for the different binding affinities of SWNTs to ssPNA and PNA/DNA duplexes that can be used for the detection of SNPs. As a proof-of-principle analyte, the SNPs in the human tumor suppressor gene TP53 have been successfully genotyped by this method. The assay does not involve any chemical modification of probes, which is simple and universal. Moreover, the LS technique and the use of a sequence-specific PNA probe impart high sensitivity and selectivity. In principle, this assay can be used to detect any kind of gene mutations by simply changing the PNA sequences of the specific probe.
研究领域:
生物化学
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分子生物学
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纳米技术
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DOI
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2023
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IF 8.2
Shuang Xiao,Peng Zhang,Guowen Zhang,Wenwen Li,Haowen Lin,Xing Hu
The activation of toll-like receptor 4 (TLR4) signaling is crucial for initiating and coordinating the immune response against infections, and is proved as a vital target for inflammatory diseases. Herein, TLR4 with sufficient amount and functional activity was generated by heterologous expression and used to investigate the mechanism of apigenin (Api)/chrysin (Chr) inhibition of TLR4 activation. The results demonstrated that Api/Chr exhibited a strong fluorescence quenching effect through a static quenching and a high binding affinity ( K a > 10 5 L·mol −1 ) with TLR4, indicating the potential of Api/Chr as a TLR4 inhibitor. Additionally, the binding of Api/Chr induced a loose and unstable conformation of TLR4 with evidence like the decreased hydrophobicity of the tryptophan microenvironment, decreased α-helix content and increased free sulfhydryl content, resulting in reduced stability of the TLR4. The computer simulations revealed that Api/Chr occupied the myeloid differentiation factor 2 (MD-2) binding region, preventing MD-2 from binding to TLR4. Furthermore, the accuracy of the binding site between Api/Chr and TLR4 was confirmed through genetic mutations . Overall, the mechanism by which Api/Chr inhibited TLR4 activation was elucidated at the macroscopic and molecular levels, providing the worthful information concerning the future therapeutic application of Api/Chr as a natural TLR4 inhibitor.
研究领域:
免疫学
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药理学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
BMC MICROBIOLOGY
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2019
·Vol. 19
·期 1
·1-8
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IF 3.287
Zhu Dekang,Zheng Mingyu,Xu Jinge,Wang Mingshu,Jia Renyong,Chen Shun,Liu Mafeng,Zhao Xinxin,Yang Qiao,Wu Ying,Zhang Shaqiu,Huang Juan,Liu Yunya,Zhang Ling,Yu Yanling,Pan Leichang,Chen Xiaoyue,Cheng Anchun
Background Riemerella anatipestifer is one of the most serious infectious disease-causing pathogens in the duck industry. Drug administration is an important method for prevention and treatment of infection in duck production, leading to widespread drug resistance in R. anatipestifer . Methods For a total of 162 isolates of R. anatipestifer, the MICs were determined for a quinolone antimicrobial agent, namely, nalidixic acid, and three fluoroquinolones, namely, ciprofloxacin, enrofloxacin and ofloxacin. The gyr A, par C, and par E gene fragments were amplified by PCR to identify the mutation sites in these strains. Site-directed mutants with mutations that were detected at a high frequency in vivo were constructed (hereafter referred to as site-directed in vivo mutants), and the MICs of these four drugs for these strains were determined. Results In total, 100, 97.8, 99.3 and 97.8% of the 137 R. anatipestifer strains isolated between 2013 and 2018 showed resistance to nalidixic acid, ciprofloxacin, enrofloxacin, and ofloxacin, respectively. The high-frequency mutation sites were detected in a total of 162 R. anatipestifer strains , such as Ser83Ile and Ser83Arg, which are two types of substitution mutations of amino acid 83 in GyrA; Val799Ala and Ile811Val in ParC; and Val357Ile, His358Tyr, and Arg541Lys in ParE. MIC analysis results for the site-directed in vivo mutants showed that the strains with only the Ser83Ile mutation in GyrA exhibited an 8–16-fold increase in MIC values, and all mutants showed resistance to ampicillin and ceftiofur. Conclusions The resistance of R. anatipestifer to quinolone agents is a serious problem. Amino acid 83 in GyrA is the major target mutation site for the fluoroquinolone resistance mechanism of R. anatipestifer .
研究领域:
兽医学
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微生物学
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分子生物学
✓ 已验证
DOI
高影响因子
Materials Today Chemistry
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2025
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IF 6.7
Hao Feng,Huicong Zhang,Weixue Zhang,Guoliang Tan,Chunxia Sun,Wei Xing,Guolei Li
s Purpose The high incidence and mortality rates of lung cancer pose a great challenge for early detection and prognostic assessment. Liquid biopsy biomarkers based on circulating tumor cells (CTC) and circulating tumor DNA (ctDNA), among others, hold promise for non-invasive detection. The aim of this study was to construct a CTC sorting system that can be used to monitor lung cancer progression and compared with ctDNA for downstream analysis. Results The sorting efficiency of the CTC sorting system for lung cancer cells was 95.70 %, and the system demonstrated the ability to monitor CTC changes and tumor progression in animals. The cutoff value of CTC detection in lung cancer was 7.5/5 mL (Area under the curve [AUC]: 0.9976, 95 % CI: 0.9946–1.000), with a sensitivity of 96.70 % and specificity of 98.41 %, effectively indicating progression. Compared with ctDNA, CTC-based analysis detected a broader genomic mutation spectrum with a concordance rate of 19.90 %, but the detection rate of the genomic mutation spectrum could be expanded by the combined test. Conclusion In this study, we successfully developed a novel clinically feasible CTC sorting system capable of monitoring lung cancer progression, which can be applied to early diagnosis, efficacy assessment, and monitoring tumor progression in lung cancer. In addition, CTC showed low concordance with mutations found in ctDNA, and CTC may have specific mutations independent of ctDNA.
研究领域:
肿瘤学
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分子生物学
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医学检验
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产品关联
DOI
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2020
·Vol. 167
·期 2
·027535
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IF 3.721
Qian Bi,Xiaoyan Gan,Ruo Yuan,Yun Xiang
Herein, a new signal amplification strategy on the basis of copper-free click chemistry-mediated cyclic ligation of DNA for detecting cancer-related mutant p53 (mp53) gene with high sensitivity is described. The complementary sandwich hybridization of the target mp53 gene with the azide-modified capture probes and the dibenzocyclooctyne-conjugated and G-quadruplex containing signal probes pulls the azide and dibenzocyclooctyne moieties into close proximity, leading to copper-free click ligation of the signal probes on the sensor surface. Subsequent thermal recycling of the target gene results in the attachment of lots of signal probes on the electrode. The many G-quadruplex segments in the signal probes then bind and confine hemin within the vicinity of the electrode surface, thereby yielding substantially amplified reduction current response for detecting the mutant target mp53 sequences with a low detection limit of 7.7 fM. This sensor can also discriminate mutant and wild gene with single base mutation and can realize target gene detection in diluted human serums. Distinct from conventional copper-mediated click chemistry and ligase-based DNA ligation, our approach is copper- and enzyme-free, making our method a simple and robust signal amplification sensing means for detecting low levels of different DNA biomarkers.
研究领域:
生物化学
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分子生物学
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医学诊断
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 6
·1-10
·
IF 5.3
Wang Lanyue,Bu Sisi,Xu Shijie,Huang Tuo,Yang Fang,Tan Qianglong,Deng Minxin,Xie Wenlin,Cai Bobo,Chen Jian
Single nucleotide polymorphism (SNP) biosensors are emerging rapidly for their promising applications in human disease prevention diagnosis, treatment, and prognosis. However, it remains a bottleneck in equipping simple and stable biosensors with the traits of high sensitivity, non-enzyme, and low cost. Double base mismatches mediated chain displacement reactions have attracted fascinating advantages of tailorable thermodynamics stability, non-enzyme, and excellent assembly compliance to involvement in SNP identification. As the base mismatch position and amount in DNA sequence can be artificially adjusted, it provides plenty of selectivity and specificity for exploring perfect biosensors. Herein, a biosensor with double base mismatches mediated catalytic hairpin assembly (CHA) is designed via one base mismatch in the toehold domain and the other base mismatch in the stem sequence of hairpin 1 (H1) by triggering CHA reaction to achieve selective amplification of the mutation target (MT) and fluorescence resonance energy transfer (FRET) effect that is composed of Cy3 and Cy5 terminally attached H1 and hairpin 2 (H2). Depending on the rationally designed base mismatch position and toehold length, the fabricated biosensors show superior SNP detection performance, exhibiting a good linearity with high sensitivity of 6.6 fM detection limit and a broad detection abundance of 1%. The proposed biosensor can be used to detect the KRAS mutation gene in real samples and obtain good recoveries between 106 and 116.99%. Remarkably, these extendible designs of base mismatches can be used for more types of SNP detection, providing flexible adjustment based on base mismatch position and toehold length variations, especially for their thermodynamic model for DNA-strand displacement reactions. Graphical Abstract
研究领域:
分子生物学
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生物传感技术
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医学诊断
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Bo Dong,Kaixuan Nie,Huanhuan Shi,Lemeng Chao,Mingyang Ma,Fengxiao Gao,Bo Liang,Wei Chen,Mengqiu Long,Zhengchun Liu
Peptide nucleic acids array technology is a method of greatly increasing the throughput of laboratory processes to efficiently perform large-scale genetic tests. Diethylene glycol-containing chiral γPNA (miniPEG-γPNA) is considered to be the best PNA derivative and one of the best candidates for gene detection, because it can hybridize DNA with greater affinity and sequence selectivity than DNA and ordinary aminoethylglycyl PNA ( aeg PNA). Herein, miniPEG-γPNA probes are synthesized by 9-fluorenylmethyloxycarbonyl (Fmoc) solid phase peptide synthesis (SPPS) in a mild condition, and a new biochip fabrication method “Film-Spotting” is invented, by which γPNA arrays with regular pattern, uniform luminance, and very low fluorescence background are obtained easily and cheaply. The miniPEG-γPNA array can effectively distinguish the full matched and mismatched targets in SSarc buffer, serum and urine, and the detection limit of complementary DNA is less than 5.97 nM. A miniPEG-γPNA array for BRCA1 gene mutation (3099delT) detection is also fabricated with a very good detection performance. This work provides an effective avenue for the diagnosis of breast cancer biomarker and expands the application of miniPEG-γPNA in the field of biochip.
研究领域:
分子生物学
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遗传学
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生物技术
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癌症诊断
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产品关联
DOI
PubMed
高影响因子
Clinical Parkinsonism & Related Disorders
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2025
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IF 1.8
Yubo Hu,Haochen Sun,Qin Jiang,Juan Wang,Shugang Zhang,Xingjian Lin
Neuronal ceroid lipofuscinosis is a rare lysosomal storage disorder that is difficult to distinguish from other diseases with similar clinical symptoms in its early stages. This article analyzes and summarizes the diagnostic process of a family affected by adult neuronal ceroid lipofuscinosis caused by CLN6 gene mutation. We collected clinical data from a 48-year-old female patient with neuronal ceroid lipofuscinosis who visited Nanjing Brain Hospital in March 2024. She has presented with corresponding symptoms since 2020. The patient underwent whole exome sequencing (WES) and other examinations. WES showed that both the patient and her elder brother, who exhibited similar symptoms of walking instability, were homozygous for a mutation in the CLN6 gene. This variant (c.856C > T: p.Leu286Phe) has not been previously reported and is classified as a Variant of Uncertain Significance (VUS) based on current American College of Medical Genetics and Genomics/Association for Molecular Pathology(ACMG/AMP) guidelines. Both of the patient’s parents displayed normal phenotypes but were found to have heterozygous mutations in the CLN6 gene. Additionally, no nuclear inclusion bodies were found in the patient’s skin tissue.
研究领域:
神经病学
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遗传学
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医学遗传学
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分子生物学
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DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）