MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Chu Chu,Linmin Lian,Wei Gao,Yaping Zang,Yanfang Zou,Yangbin Lv,Chenyue Li,Shengqiang Tong,Jizhong Yan,Suhong Chen
An efficient off-line and on-line synergistic preconcentration strategy which involves combining micro matrix solid-phase dispersion (MSPD) with electrokinetic supercharging (EKS) in capillary zone electrophoresis (CZE) has been developed for the determination of two alkaloids, lycorine and galanthamine, in complicated matrix samples. This method was comprehensively optimized in terms of the buffer condition, the leading electrolyte (LE), terminating electrolyte (TE), the sample injection in EKS preconcentration, as well as the dispersing sorbent, the sample/dispersing sorbent mass ratio, grinding time, eluting solvent in MSPD procedure. Under the optimum conditions, lycorine and galanthamine, achieved about 1500- and 1200-fold sensitivity improvement respectively when compared to the ordinary injection. A satisfactory correlation coefficient (R 2 > 0.9982) was obtained in the range of 10–500 ng/mL. The limits of detection (LODs) for lycorine and galanthamine were 6.0 ng/mL and 8.0 ng/mL, respectively. Moreover, the proposed method was successfully applied in two real samples, rat fecal samples and Bulbus Lycoridis Radiatae, to demonstrate its feasibility. Recoveries in spiked fecal and plant samples were 89.51% to 107.4%. The result showed that the new developed method possessed the great potential in analyzing trace alkaloids in complicated samples matrix.
研究领域:
分析化学
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药理学
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DOI
高影响因子
STAR Protocols
·
2025
·
IF 1.3
Weihao Zhao,Ziqian Liu,Zhenghao Li,Hao Qian,Jing Hu
Summary Neuronal cell cultures are highly sensitive to their microenvironment, particularly the choice of coating substrate. Here, we present a protocol to isolate extracellular matrix (ECM) from mouse brain tissue, providing a native coating material that closely mimics in vivo conditions. We detail decellularization steps, guidelines for measuring ECM quality, and instructions for co-culturing neuronal cells on the resulting substrate. By promoting improved neuronal survival, growth, and differentiation, this protocol has broad implications for in vitro neurobiological research and downstream applications.
研究领域:
神经科学
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细胞生物学
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组织工程
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产品关联
DOI
PubMed
高影响因子
Bioactive Materials
·
2024
·
IF 18
Yinghao Li,Dongling Xiao,Weixi Yan,Meilin Jiang,Ju Tan,Zhongliang Qin,Jingting Zhou,Yue Sun,Mingcan Yang,Guanyuan Yang,Yawei Gu,Yong Liu,Chuhong Zhu
Timely and stable sealing of uncontrolled high-pressure hemorrhage in emergency situations outside surgical units remains a major clinical challenge, contributing to the high mortality rate associated with trauma. The currently widely used hemostatic bioadhesives are ineffective for hemorrhage from major arteries and the heart due to the absence of biologically compatible flexible structures capable of simultaneously ensuring conformal tough adhesion and biomechanical support. Here, inspired by the principle of chromatin assembly, we present a tissue-conformable tough matrix for robust sealing of severe bleeding. This hierarchical matrix is fabricated through a phase separation process, which involves the in-situ formation of nanoporous aggregates within a microporous double-network (DN) matrix. The dispersed aggregates disrupt the rigid physical crosslinking of the original DN matrix and function as a dissipative component, enabling the aggregate-based DN (aggDN) matrix to efficiently dissipate energy during stress and achieve improved conformal attachment to soft tissues. Subsequently, pre-activated bridging polymers facilitate rapid interfacial bonding between the matrix and tissue surfaces. They synergistically withstand considerable hydraulic pressure of approximately 700 mmHg and demonstrate exceptional tissue adhesion and sealing in rat cardiac and canine aortic hemorrhages, outperforming the commercially available bioadhesives. Our findings present a promising biomimetic strategy for engineering biomechanically compatible and tough adhesive hydrogels, facilitating prompt and effective treatment of hemorrhagic wounds.
研究领域:
生物材料
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生物医学工程
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组织工程
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止血
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产品关联
DOI
PubMed
高影响因子
GONDWANA RESEARCH
·
2025
·
IF 8.6
Taishuo Zhang,Wenfu Wang,Xiang Zhi,Xiaoqian Ye,Rui Wang,Jiarui Zhao,Jiamin Dai,Hong Qiang,Peng Liu,Hanzhong Jia
This study investigated the microbial colonization, bacterial community and potential risks in natural (montmorillonite (MMT)) and artificial substrates (activated carbon (AC), polypropylene (PP) and polystyrene (PS)) by microcosm incubations in surface water. Biofilm biomass and polysaccharide content indicate that the colonization potential of microbes on different substrates followed the order of AC > MMT > PP > PS. High-throughput sequencing results reveal that microbes selectively colonized various substrates and formed unique bacterial community. Compared to the artificial substrate, natural substrate enriched with high abundance but low diversity of microbial community. In addition, bacterial community on natural substrates exhibited stronger nitrogen cycling function than artificial substrates, whereas bacterial community on artificial substrates (especially microplastics) exhibited stronger carbon cycling functions, such as aromatic_compound_degradation and hydrocarbon_degradation. Also, Brevundimonas diminuta , Sphingomonas paucimobilis, Paracoccus sanguinis and other twenty-five species of pathogenic bacteria were identified in various substrates and their abundance was higher on MMT than on other substrates. However, many more species of pathogenic bacteria on microplastic are associated with human diseases, implying a higher environmental risk. These results indicated that microbial community composition and function on natural and artificial substrates exhibited substrate-driven differences, highlighting the lower concerns of artificial substrates than natural ones with associated biofilms in surface water.
研究领域:
环境科学
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微生物学
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生态学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
·
IF 8.5
Hailin Zhang,Baolei Huang,Wenjing Song,Wenfang Liu,Ye Tian,Zhuhao Tan,Xingqiang Zhu,Yu Niu,Wei Zhai,Anmin Wang,Lianghong Peng,Li Ren
Suture-free corneal repair strategy has become a focus in the field of corneal injury treatment due to its minimal invasiveness and high efficiency. This study introduces a dual-layer convex patch for suture-free repair of ocular trauma, comprising a thermosensitive gelatin/alginate adhesive layer and a convex collagen substrate layer. This design addresses critical limitations of conventional adhesives, particularly their difficulty in forming symmetric curvature during application and insufficient bonding strength under intraocular pressure fluctuations. In vitro studies demonstrated that the patch possessed superior transmittance (above 80 %) and high burst pressure (above 200 mmHg). This dual-layer convex patch can reduce surgical time by two-thirds compared to the suture group. In a lamellar keratoplasty model, the patch promoted epithelial healing, enhanced stroma regeneration, and inhibited the formation of stromal scars. For irregular large-sized corneal penetrating injuries, the patch group achieved rapid wound sealing, promoted epithelial healing, and showed more symmetrical corneal curvature and 90 % reduction in neovascularization compared to fibrin. The superior performance of the dual-layer convex patch makes it a promising alternative for the suture-free treatment of corneal trauma, achieving both corneal stroma defects repair and irregular penetrating injuries healing.
研究领域:
生物医学工程
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眼科
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组织工程
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生物材料
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再生医学
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产品关联
DOI
PubMed
高影响因子
Advanced Healthcare Materials
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2025
·
IF 9.6
Junlin Chen,Qingtao Li,Chuhan Lv,Hongbo Yu,Cheng Dai,Ye Hu,Hua Dong
Articular cartilage repair remains challenging due to the inherent poor self-healing capacity and acute inflammation resulting from the over expression of intracellular reactive oxygen species (ROS). Although microfracture is frequently used in practical surgery, the newborn cartilage always exhibits fibrosis, mainly attributed to the weak ROS scavenging and mismatched microenvironment that fails to induce chondrogenic differentiation of bone marrow mesenchymal stem cells (BMSCs). Herein, a novel simvastatin (SIM)-incorporated kelp and cartilage acellular matrix hybrid (labeled as SIM@KACM/CACM) microgel assembly with enhanced ROS scavenging, endogenous BMSC recruitment, and chondrogenic differentiation capabilities is constructed to improve microfracture-based articular cartilage repair. The kelp acellular matrix exhibits low immunogenicity, high biocompatibility, and ROS scavenging properties, whilst the sustained SIM release promotes BMSC recruitment from the bone marrow cavity. Meanwhile, the microenvironment and bioactive factors conferred by CACM facilitate the BMSC proliferation and differentiation into hyaline cartilage. In addition, the construction of porous microgel assembly via dynamic Schiff's bonds endows excellent injectability and tissue adhesion, which not only enhances its retention in the defect site after injection but also provides abundant sites for BMSC anchoring and infiltration. Due to the synergetic effect of the above-mentioned multiple factors, the articular cartilage repair is improved dramatically.
研究领域:
医学
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组织工程
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再生医学
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产品关联
DOI
PubMed
高影响因子
Science China-Technological Sciences
·
2021
·Vol. 64
·期 6
·1285-1294
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IF 3.572
Rong Yan,Zhang Zhen,He ChaoLiang,Chen XueSi
The response of extracellular matrix (ECM) to dynamic cell signals is of great significance for the regulation of cell behavior. In the present study, we prepared a type of matrix metalloproteinase (MMP)-sensitive degradable hydrogels (MSDHs) via the catalyst-free o -phthalaldehyde (OPA)/amine cross-linking reaction between o -phthalaldehyde-grafted four-arm poly(ethylene glycol) (4aPEG-OPA) and an MMP-sensitive degradable peptide. The gelation rates and storage moduli of MSDHs and the MMP-insensitive hydrogels (MIHs) based on an MMP-insensitive scramble peptide were comparable and dependent on the concentrations of precursor polymers. MSDHs were degradable while MIHs were stable in the presence of proteinase in vitro . The degradation of MSDHs was obviously faster than that of MIHs after subcutaneous injection into rats. In addition, both types of poly(ethylene glycol)/peptide hydrogels displayed excellent cytocompatibility in vitro , and showed good histocompatibility in vivo in the subcutaneous layer of rats. Furthermore, the proliferation of several MMP-expressing cell lines including MDA-MB-231 cells within MSDHs was obviously faster than that in MIHs, indicating the influence of metabolism-mediated scaffold degradation on the cell proliferation. This study provides a new biocompatible and biodegradable 3D cell culture interactive platform for regulation of cell behavior.
研究领域:
生物医学工程
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组织工程
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细胞生物学
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材料科学
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DOI
高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2025
·
IF 3.9
Kangxin Bai,Yang Liu,Zhuangzhi Li,Jiajie Shi,Zhenggong Wang,Jian Jin
The separation of C2H4/C2H6 is crucial for the efficient production of C2H4. The energy-efficient membrane technology has great potential in this separation. However, the similar physical properties of the two molecules make them extremely difficult in membrane separation. Herein, a series of nanosized MOF-808 particles were incorporated into a polyimide matrix to fabricate mixed matrix membranes (MMMs) for C2H4/C2H6 separation. The well-defined pore architecture of MOF-808 enables effective C2H4 purification through molecular sieving, while the restricted diffusion of C2H6 molecules within the MOF-808 channels facilitates remarkable kinetic separation. Importantly, the nanosized MOF-808 can be dispersed more evenly in the polymer matrix, significantly improving the compatibility between the polymer matrix and filler, thereby maximizing the sieving effect of MOF-808. The resulting nanosized MOF-808-based MMM demonstrates a high C2H4 permeability of 1187 barrer and a C2H4/C2H6 selectivity of 2.8, which has exceeded most of the MMMs reported so far and surpassed the 2013 upper bound. Our MMMs also show excellent long-term stability and antiplasticization performance. Our work highlights the effectiveness of enhancing the separation performance of MMMs by using reducing nanosized MOF-808, providing valuable insights for the design of MMMs for C2H4/C2H6 separation.
研究领域:
膜技术
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气体分离
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材料科学
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化学工程
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纳米技术
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产品关联
DOI
高影响因子
Collagen and Leather
·
2024
·Vol. 6
·期 1
·1-13
·
IF 0
Wang Xiang,Jiang Yujia,Sun Xiaoqin,Yue Chongxia,Li Zhengyong,Wu Yao
Acellular dermal matrix (ADM) is one of the most promising scaffold materials due to its ability to retain natural extracellular matrix structure. Micronized acellular dermal matrix (mADM) was prepared with no intact cell nuclei and preserved growth factors by High Hydrostatic Pressure (HHP) approach. And mADM-collagen wound dressings were developed with different proportion of type I collagen and recombinant humanized type III collagen. The porous structure of the mADM-collagen wound dressings made them a good candidate for preventing excessive fluid accumulation, while the collagens with gel-like texture combined with mADM powder to form pasty texture wound dressing, which preserving the moisture at the wound site. Moreover, the paste texture of the mADM-collagen wound dressing was easy to reshape to conform any wound shapes and body contours. Furthermore, the resulted mADM-collagen wound dressings showed good biocompatibility by supporting fibroblasts adhesion and proliferation in vitro. Subsequently, a murine model of full-thickness skin wounds was employed to assess its effects on wound healing. Notably, mADM-75% Col-I exhibited superior effects throughout the wound healing process, specifically it promoted neovascularization, skin appendage growth and new skin regeneration. This formulation closely mimicked the collagen ratio found in healthy skin, facilitating the favorable wound repair. These results indicated the superior performance of this mADM-collagen wound dressing providing an optimal environment for wound healing. Graphical Abstract
研究领域:
生物材料
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组织工程
·
创伤修复
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DOI
BIOMATERIALS
·
2019
·
IF 10.273
Chuanchuan Lin,Bailong Tao,Yiman Deng,Ye He,Xinkun Shen,Rong Wang,Lu Lu,Zhihong Peng,Zengzilu Xia,Kaiyong Cai
Bone marrow derived mesenchymal stromal cells (BMSCs) migration to injury site is a prevalent event in tissue repair process after damage occurrence. The migration process is always accompanied with matrix stiffness change. In this study, sodium alginate hydrogels with different stiffness and Transwell chambers with gradient chemical factors were employed to mimic tissue repair in vivo . In this work, in the stiffness range of 1–20 kPa, BMSCs in stiffer matrix showed higher migration speed compared to those in softer matrix. Moreover, stiffer matrix decreased the nuclear stiffness of BMSCs and reduced the expression of lamin A/C, which playing a main role in the regulation of nuclear stiffness. Furthermore, it was found that BMSCs fitted environment by selecting migration strategy. This study provides a novel platform for the investigation of BMSCs migration to mimic the natural tissue repair process.
研究领域:
细胞生物学
·
组织工程
·
生物力学
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DOI
PubMed
高影响因子
BIO-MEDICAL MATERIALS AND ENGINEERING
·
2015
·
IF 1.091
Wang Xiaotong,Ma Bing,Chang Jiang
BACKGROUND: Vascular extracellular matrices (vECMs) have shown potential for small-diameter blood vessel tissue engineering applications. However, problems such as chemical instability and easy calcification are still remained. Chemical crosslinking using crosslinkers such as glutaraldehyde (GA) can improve mechanical properties and proteolysis resistance of vECMs, but leads to calcification and cytotoxicity. Procyanidins (PC) can crosslink ECMs with anti-calcification property and cytocompatibility, but the mechanical properties and chemical stability are unsatisfactory. OBJECTIVE: A novel co-crosslinking technique using PC and GA was developed, which combines the advantages of both PC and GA for enhancing mechanical properties and stability of vECMs with reduced calcification and cytotoxicity. METHODS: Fresh carotid were decellularized and then crosslinked by PC and subsequent GA for 6 h respectively. The mechanical properties, dynamic release of PC, enzymatic degradation, calcification and cytotoxicity of crosslinked samples were evaluated. RESULTS: The co-crosslinked vECMs showed enhanced tensile strength, chemical and biological stability, comparable anti-calcification property as compared to pure PC-crosslinked samples. Cytotoxicity assay showed that the co-crosslinked vECMs were cytocompatible for supporting the adhesion and proliferation of HUVECs. CONCLUSIONS: Co-crosslinking with PC and GA might be a useful method for preparation of vECM scaffolds with potential applications in small-diameter blood vessel tissue engineering.
研究领域:
组织工程
·
生物材料科学
·
生物医学工程
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DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2022
·Vol. 51
·期 12
·4685-4694
·
IF 4.569
Guixian Li,Yu Xue,Qinan Mao,Lang Pei,Hong He,Meijiao Liu,Liang Chu,Jiasong Zhong
Luminescent thermometers provide a non-contact method of probing temperature with high sensitivity and response speed at the nanoscale. Synergistic photoluminescence from different activators can realize high sensitivity for luminescent thermometers by finely selecting ions with specific crystallographic sites. Herein, the more temperature-sensitive Mn4+ and the less-sensitive Eu3+ (or Sm3+) activators are co-doped into a Ca2GdSbO6 matrix to form an effective thermometer, where Mn4+ and Eu3+ (or Sm3+) ions occupy the Sb5+ and Gd3+ sites, respectively. The co-doping of Eu3+ ions or Sm3+ ions leads to lattice expansion of Ca2GdSbO6 matrix and a tuned narrow emission from deep-red to orangish-red. According to the ratio of luminescence intensity, the maximal Sa and Sr values are 0.19 K−0 (347 K) and 1.38% K−( (420 K) for Ca2GdSbO6:Mn4+/Eu3+ probe and 0.26 K−p (363 K) and 1.55% K−( (430 K) for Ca2GdSbO6:Mn4+/Sm3+ probe thermometers, respectively. In addition, thermometers based on Mn4+ emission lifetimes can provide the highest relative sensitivity of 1.47% K−s at 425 K. Thus, the highly-temperature-sensitive Ca2GdSbO6:Mn4+/(Eu3+ or Sm3+) phosphor is a promising candidate for practical luminescence thermometers.
研究领域:
材料科学
·
无机化学
·
光学材料
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纳米技术
·
生物传感
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）