Science Advances
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2025
·Vol. 11
·期 19
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IF 12.5
Hengjia Zhu,Qiyu Deng,Junzhi Li,Ling Yang,Hegeng Li,Zhipeng Zhao,Zuochen Wang,Chunlin Pang,Yiyuan Zhang,Vincent Chi-Hang Lui,Wei Li,Xiaobo Yin,Liqiu Wang
Precision processing of various liquids while maintaining their purity holds immense potential for many applications. However, liquids tend to leave residues that contaminate handling tools and compromise volumetric precision, necessitating contactless strategies to prevent liquid loss. Biological and chemical samples carried by fluids can be sensitive to physical stimuli, demanding mild but effective means to preserve integrity. Here, we report a sound-controlled fluidic processor for complete and well-controlled microfluidic functions, including moving, merging, mixing, and cleaving, in contactless and harmless manners. The processor generates an acoustophoretic force field that serves as a versatile toolbox for manipulating droplets with surface tension from 17.9 to 72 millinewtons per meter and volume from 1 nanoliter to 3 milliliters, offering a wealth of operations crucial to fundamental biomedical and chemical practices.
研究领域:
生物医学工程
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化学工程
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声学
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产品关联
DOI
PubMed
高影响因子
MACROMOLECULAR BIOSCIENCE
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2023
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IF 4.6
Chengdian He,Xiong He,Yi Zhang,Xiaofeng Han,Yujun Yang,Yong Shen,Teng Wang,Qing Wu,Yukun Yang,Wei Xu,Jin Bai,Zhenyu Wang
Transdermal drug delivery system (TDDS) has attracted much attention in the pharmaceutical technology area. However, the current methods are difficult to ensure penetration efficiency, controllability, and safety in the dermis, so its widespread clinical use has been limited. This work proposes an ultrasound-controlled monodisperse lipid vesicles (U-CMLVs) hydrogel dressing, which combines with ultrasound to form TDDS. Using microfluidic technology, prepare size controllable U-CMLVs with high drug encapsulation efficiency and quantitative encapsulation of ultrasonic response materials, and even uniform mix them with hydrogel to prepare the required thickness of dressings. The high encapsulation efficiency can ensure sufficient dosage of the drugs and further realize the control of ultrasonic response through quantitative encapsulation of ultrasound-responsive materials. Using high frequency (5 MHz, 0.4 W cm −2 ) and low frequency (60 kHz, 1 W cm −2 ) ultrasound to control the movement and rupture of U-CMLVs, the contents not only penetrate the stratum corneum into the epidermis but also break through the bottleneck of penetration efficiency, and deep into the dermis. These findings provide the groundwork for deep, controllable, efficient, and safe drug delivery through TDDS and lay a foundation for further expanding its application.
研究领域:
药学
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生物医学工程
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纳米医学
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产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
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2023
·
IF 14
Xiaoyu Han,Jieliang Shen,Shuyu Chen,Zhengwei Cai,Ying Zhu,Weiwei Yi,Kai Li,Weiye Cai,Bailong Tao,Wenguo Cui,Dingqun Bai
Spatiotemporal Immune disorder is a key factor leading to the failure of bone tissue healing. It is of vital importance to accurately suppress excessive peak immune response within 24-48 h of the injury and so regulate the spatiotemporal osteoimmune disturbance of bones. In this study, Ultrasound Controlled “Explosive” (UCE) hydrogels were prepared from gelatin-hyaluronic acid methacrylate hydrogels loaded with resveratrol nanobubbles produced by double emulsification through a condensation reaction . Such materials innovatively enable ultrasound-controlled RES release for precise regulation of spatiotemporal osteoimmune disorders. Under an ultrasonic power level of 1.5 W/cm 2 , the rate of effectively released RES through the blast of UCE hydrogels reached 38.14 %. And compared with the control group, the in vivo inhibition of inflammation and osteogenesis effects of UCE hydrogels were more effective, respectively. As suggested by the results, the excessive local inflammatory response was inhibited by the release of resveratrol, the temporospatial disorder of bone immune was precisely regulated, and as a result, the process of bone repair was accelerated. Altogether, this study confirms that the newly created UCE Hydrogels effectively promote bone repair by intervening peak inflammation during the early phase of fracture healing .
研究领域:
生物材料
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生物医学工程
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组织工程
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免疫学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2023
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IF 9.5
Cancan Du,Ruiqi Wu,Wenqiang Yan,Jingchao Fang,Wenli Dai,Yiqun Wang,Jin Cheng,Xiaoqing Hu,Yingfang Ao,Xiaolong Liang,Zhenlong Liu
Rotator cuff tear (RCT) is a prevalent shoulder injury that poses challenges for achieving continuous and functional regeneration of the tendon-to-bone interface (TBI). In this study, we controlled the delivery of growth factors (GFs) from liposomal nanohybrid cerasomes by ultrasound and implanted three-dimensional printed polycaprolactone (PCL) scaffolds modified with polydopamine loaded with bone marrow mesenchymal stem cells (BMSCs) to repair tears of the infraspinatus tendon in a lapine model. Direct suturing (control, CTL) was used as a control. The PCL/BMSC/cerasome (PBC) devices are sutured with the enthesis of the infraspinatus tendon. The cerasomes and PCL scaffolds are highly stable with excellent biocompatibility. The roles of GFs BMP2, TGFβ1, and FGF2 in tissue-specific differentiation are validated. Compared with the CTL group, the PBC group had significantly greater proteoglycan deposition (P = 0.0218), collagen volume fraction (P = 0.0078), and proportions of collagen I (P = 0.0085) and collagen III (P = 0.0048). Biotin-labeled in situ hybridization revealed a high rate of survival for transplanted BMSCs. Collagen type co-staining at the TBI is consistent with multiple collagen regeneration. Our studies demonstrate the validity of biomimetic scaffolds of TBI with BMSC-seeded PCL scaffolds and GF-loaded cerasomes to enhance the treatment outcomes for RCTs.
研究领域:
生物医学工程
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组织工程
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干细胞研究
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骨科手术
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DOI
PubMed
高影响因子
Acta Biomaterialia
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2022
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IF 10.633
Zixin Zhu,Xue Gou,Laiyi Liu,Tian Xia,Jiayi Wang,Yimeng Zhang,Chenjun Huang,Wei Zhi,Ran Wang,Xiaohong Li,Shengnian Luo
Wound healing from bacterial infections is one of the major challenges in the biomedical field. The traditional single administration methods are usually accompanied with side effects or unsatisfactory efficacy. Herein, we design dynamically evolving antibacterial and repair-promoting nanocomposites (NCs) by in situ self-assembling of zeolitic imidazolate framework-8 (ZIF-8) on the surface of barium titanate (BTO), and further loading with a small amount of ciprofloxacin (CIP). The new strategy of combining pH-stimulated drug delivery and ultrasound-controlled sonodyamics has the potential to dynamically evolve in infected wound sites, offering a multifunctional therapy. In vitro study demonstrates that the enhancement generation of reactive oxygen species through the sonodynamic process due to the heterostructures and a small amount of CIP released in an acidic environment are synergistically antibacterial, and the inhibition rate was >99.9%. In addition, reduced sonodynamic effect and Zn 2+ generated along with the gradual degradation of ZIF-8 simultanously promote cell migration and tissue regeneration . The in vivo study of full-thickness skin wounds in mouse models demonstrate a healing rate of 99.3% could be achieved under the treatment of [email protected] /CIP NCs. This work provides a useful improvement in rational design of multi-stimulus-responsive nanomaterials for wound healing. Statement of significance A novel piezoelectric nanocomposite was proposed to realize sonodynamic therapy and pH-stimulated drug releasing simultaneously in wound healing treatment. The dynamically evolving structure of the piezoelectric nanocomposite in acidic microenvironment has been theoretically and experimentally verified to contribute to a continuous variation of sonodymanic strength , which accompanied with the gradual releasing of drug and biocompatible Zn 2+ effectively balanced antibacterial and repair-promoting effects. Both of the in vitro and in vivo study demonstrated that the strategy could significantly accelerate wound healing, inspiring researchers to optimize the design of multi-stimulus-responsive nanomaterials for various applications in biomedical and biomaterial fields.
研究领域:
生物医学工程
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纳米技术
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伤口愈合
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抗菌材料
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组织工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）