COLLOIDS AND SURFACES B-BIOINTERFACES
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2016
·
IF 3.902
Lele Wang,Jing Zhang,Meijia Song,Baocheng Tian,Keke Li,Yan Liang,Jingtian Han,Zimei Wu
Based on targeted amphiphilic block copolymer N -acetyl glucosamine-poly (styrene-alt-maleic anhydride) 58 -b-polystyrene 130 (NAG-P(St-alt-MA) 58 -b-PSt 130 ), a pH/redox dual-triggered shell-crosslinked polymeric micelle system was constructed. The shell-crosslinked micelles (CLM) were prepared by post-crosslinking method to regulate drug release kinetics using cystamine as linkers between carboxy groups of the shell. Compared with non-crosslinked micelles (NCLM), CLM showed spherical shapes with little increased mean diameter of 102.40 ± 0.54 nm, low polydispersity index (PDI) of 0.19 ± 0.36, enlarged zeta potential value from −41.46 ± 0.99 to −9.31 ± 0.50 mV, indicating the successful modification of disulfide bonds in shell. In vitro drug release study clearly exhibited a pH and redox dual-sensitive drug release profile with significantly accelerated drug release under pH 5.0 and 10 mM GSH conditions (46.84% in 96 h) without burst release. Both CLM and NCLM showed quite different release profiles between physiological (pH 7.4) and tumoral microenvironment (pH 5.0), effectively avoiding the premature drug leakage and realizing on-demand drug release. The MTT assay implied that CLM presented a time- and concentration-dependent manner to inhibit proliferation of A549 and MCF-7 cells and much lower IC 50 values in comparison with that of NCLM after 72 h incubation. Both FCM and CLSM results showed that CLM displayed much higher cellular uptake efficiency and anti-tumor activities than NCLM and free DOX. CLM and NCLM could be internalized by energy-dependent endocytosis mechanism due to similar surface properties. Overall, this dual-stimuli triggered micelle system provided a promising tumor-responsive platform for cancer therapy.
研究领域:
药剂学
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肿瘤学
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生物材料学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2014
·
IF 5.9
Pingsheng Huang,Jinjian Liu,Weiwei Wang,Chen Li,Junhui Zhou,Xue Wang,Liandong Deng,Deling Kong,Jianfeng Liu,Anjie Dong
Enabling nanocarriers to complete the sophisticated journey from the initial injection site to the targeted tumor cells and achieve “spatiotemporally pinpointed” drug release intracellularly is a challenging task in anticancer drug delivery. Herein, versatile shell-cross-linked nanoparticles (SCNPs) were prepared by one-step assembly of triblock zwitterionic copolymers, polycarboxybetaine methacrylate-block-poly(N-(2-(2-pyridyl disulde) ethyl methacrylamide-block-poly(2-(diisopropylamino) ethyl methacrylate) (PCB-b-PDS-b-PDPA, termed as PCSSD), which was well-defined via reversible additive fragment transfer (RAFT) polymerization, followed by functionalization with Arg-Gly-Asp (RGD). Thus, the RGD-PCSSD SCNPs cooperatively combine the ultra pH-sensitive PDPA core for efficient drug loading and pH-responsive drug release, the disulfide-cross-linked PDS shell that prevents premature drug release, the zwitterionic PCB corona to stabilize the SCNPs and prolong its systemic circulation, the RGD ligand for active tumor targeting and receptor-mediated endocytosis. Doxorubicin (DOX) was loaded as a model medicine (termed as RGD-PCSSD/DOX SCNPs). The dual-sensitivity studies showed that the pH-sensitivity of PDPA core could be adjusted by the shell-cross-linking density, accompanied by better control over premature drug release. Furthermore, results obtained by flow cytometry and fluorescence microscopy analysis demonstrated that once the RGD-PCSS10D/DOX SCNPs were internalized into tumor cells via receptor-mediated endocytosis, boost drug release was observed with considerable cytotoxicity in vitro. The results of ex vivo imaging studies further confirmed the successful drug delivery from the injection site to the tumor tissue. In summary, the well-constructed RGD-PCSS10D/DOX SCNPs with cooperative multifunctionality showed great potential as novel nanocarriers for tumor targeted anticancer drug delivery.
研究领域:
药剂学
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肿瘤学
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生物材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Soft Matter
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2011
·Vol. 7
·期 11
·5348-5352
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IF 4.457
Yin Wang,Guohua Jiang,Ming Zhang,Lina Wang,Rijing Wang,Xinke Sun
A series of novel shell cross-linked nanocapsules were facilely fabricated in an inverse miniemulsion reversible addition–fragmentation chain transfer (RAFT) system, in which poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA) acted as a stabilizer and macroinitiator. The as-prepared nanocapsules with PDMAEMA cores and poly(methylacrylic acid) (PMAA) shells, had a size of 220 nm and can be used as a carrier for the encapsulation of hydrophilic components such as inorganic salts.
研究领域:
材料科学
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化学工程
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
MATERIALS RESEARCH INNOVATIONS
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2016
·
IF 0
Ling Liu,Xiuli Wang,Lisi Xie,Sile He
Shell cross-linked core–shell nanoparticles (SCCSN) with organic montmorillonite as the core and cross-linked polymethacrylate (PMMA) as the shell were prepared through seeded emulsion polymerisation. Scanning electron microscopy and transmission electron microscopy images of SCCSN show that each of the SCCSN is spherical with core–shell structure. The thermal properties and rheological behaviour of polypropylene (PP)/SCCSN composites with different proportions of SCCSN were systematically studied using a thermogravimetric analyser and an RT-2000 high-pressure capillary rheometer, respectively. The existence of SCCSN improves the thermal properties of PP, and the thermostability of the composite increases with increasing SCCSN content. The formed composite is a non-Newtonian pseudoplastic fluid with a similar processing behaviour to PP. The rheological parameters (e.g. shear stress, apparent viscosity and flow activation energy) of the composite decrease as SCCSN fills in. These data indicate that using SCCSN does not affect the processing fluidity of PP but improves its processability.
研究领域:
材料科学
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热分析
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高分子化学
✓ 已验证
产品关联
DOI
Polymer Chemistry
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2010
·Vol. 1
·期 10
·1638-1643
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IF 0
Yin Wang,Guohua Jiang,Xinke Sun,Miaojun Ding,Hongyuan Hu,Wenxing Chen
Shell cross-linked polymeric nanoparticles were prepared through an interfacially confined copolymerization of styrene and disulfide cross-linker in a reversible addition-fragmentation chain transfer (RAFT) miniemulsion system using poly(2-(dimethylamino) ethyl methacrylate) (PDMAEMA) as stabilizer and macroinitiator. 1H NMR, FT-IR, and FE-SEM have been applied to investigate the structures and morphologies of the resultant nanoparticles. Fluorescent spectra results show that the as-prepared samples have fluorescent properties, which may be used as a fluorescent carrier for control release of guest molecules.
研究领域:
高分子化学
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纳米医学
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药物递送
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生物材料
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聚合物科学
✓ 已验证
DOI
危险品化学品经营许可证（带存储）