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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES · 2021 · IF 6.953

Preparation of chitosan-SiO2 nanoparticles by ultrasonic treatment and its effect on the properties of starch film

Jumin Hou,Xiaoxia Yan

The ultrasonic treatment could decrease the particles size of chitosan-SiO 2 particles. The EDS of samples with ultrasonic treatment for 0 min and 10 min indicated that the SiO 2 was present in prepared chitosan-SiO 2 particles. When the ultrasonic time reached 10 min, the chitosan-SiO 2 nanoparticles formed and the mean size was 506.7 ± 3.42 nm and the Zeta potential of the chitosan-SiO 2 nanoparticles was greater than 30 mV, showing the suspension was a stable dispersed liquid. The chitosan-SiO 2 nanoparticles were used as reinforcement in thermoplastic starch films to further verify their performance in enhanced films. The water contact angle of thermoplastic starch film reinforced with chitosan-SiO 2 nanoparticles was 44.13 ± 5.02° and had a good mechanical property with tensile strength of 8.91 ± 0.49 MPa. This study indicates that chitosan-SiO 2 nanoparticles could be used as a reinforcement to prepare thermoplastic starch films and promote the application of chitosan nanoparticles in nanocomposite films.

CAS / MDL: 7631-86-9
研究领域: 材料科学 · 食品科学
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JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A · 2018 ·Vol. 106 ·期 5 ·1223-1235 · IF 3.231

In vivo immuno-reactivity analysis of the porous three-dimensional chitosan/SiO2 and chitosan/SiO2/hydroxyapatite hybrids

Mengxia Guo,Yifan Dong,Jiangwei Xiao,Ruicai Gu,Maochao Ding,Tao Huang,Junhua Li,Naru Zhao,Hua Liao

Inorganic/organic hybrid silica-chitosan (CS) scaffolds have promising potential for bone defect repair, due to the controllable mechanical properties, degradation behavior, and scaffold morphology. However, the precise in vivo immuno-reactivity of silica-CS hybrids with various compositions is still poorly defined. In this study, we fabricated the three-dimensional (3D) interconnected porous chitosan-silica (CS/SiO 2 ) and chitosan-silica-hydroxyapatite (CS/SiO 2 /HA) hybrids, through sol–gel process and 3D plotting skill, followed by the naturally or freeze drying separately. Scanning electron microscopy demonstrated the hybrids possessed the uniform geometric structure, while, transmission electron microscopy displayed nanoscale silica, or HA nanoparticles dispersed homogeneously in the CS matrix, or CS/silica hybrids. After intramuscular implantation, CS/SiO 2 and CS/SiO 2 /HA hybrids triggered a local and limited monocyte/macrophage infiltration and myofiber degeneration. Naturally dried CS/SiO 2 hybrid provoked a more severe inflammation than the freeze-dried ones. Dendritic cells were attracted to invade into the implants embedded-muscle, but not be activated to prime the adaptive immunity, because the absence of cytotoxic T cells and B cells in muscle received the implants. Fluorescence-activated cell sorting (FACS) analysis indicated the implanted hybrids were incapable to initiate splenocytes activation. Plasma complement C3 enzyme linked immunosorbent assay (ELISA) assay showed the hybrids induced C3 levels increase in early implanting phase, and the subsequent striking decrease. Thus, the present results suggest that, in vivo , 3D plotted porous CS/SiO 2 and CS/SiO 2 /HA hybrids are relatively biocompatible in vivo, which initiate a localized inflammatory procedure, instead of a systematic immune response. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1223–1235, 2018.

CAS / MDL: 75-15-0 78-10-4 77-86-1
研究领域: 生物材料 · 组织工程 · 免疫学 · 生物医学材料
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